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Gene information
General information
TC013683 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 5029174..5032243
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013683
    iBeetle screen iB_02183
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013683 RA
      MYRLPSTMR SVALSKANR LSQIQRWYA KDVRFGPEV 
      RALMLQGVD VLADAVAVT MGPKGRNVI IEQSWGSPK 
      ITKDGVTVA KGVELKDKF QNIGARLVQ DVANNTNEE 
      AGDGTTTAT VLARSIAKE GFENLGKGA NPVEIRKGI 
      MLAVEKITE TLKTLSKPV TTPEEICQV ATISANGDQ 
      SVGNLIADA MKKVGKEGV ITVKDGKTL HDELEVIEG 
      FKFDRGYIS PYFVNTSKG AKVEYQDAL ILLSEKKIS 
      SVQSIVPAL ELANMQKKP LIIVAEDID GEALTTLVV 
      NRLRIGLQV AAVKAPGFG DNRKATLQD MAIATGGIV 
      FGDEANIVK LEDVQLSDL GQVGEIVIT KDDTLILKG 
      KGKKDDISK RAEQIKDQI ENTTSEYEK EKLQERLAR 
      LASGVALLK VGGSSEVEV NEKKDRVTD ALNATRAAV 
      EEGIVPGGG TALLRCSGS LDGLKPGNN DQAIGIEIV 
      KRALKVPCM TIAKNAGVD GATVVAKIE QQQGDYGYD 
      ALNNEYVNM FERGIIDPT KVVRTALID ASGVASLLT 
      TAEAVITEI PKEEPPIPS GGMGGMGGM GGMGGMM
    CDS sequence
    • >TC013683 RA
      atgtatcgt ttaccgtcg accatgcga tcagtcgcc 
      ctcagcaaa gccaacaga ttatcccaa atccagcgt 
      tggtacgcc aaagacgtc cgattcggc cccgaagtc 
      cgggccctg atgctccaa ggcgtcgac gttcttgca 
      gacgctgtg gcggtcacc atgggccct aagggccgt 
      aacgtgatc atcgaacag tcgtggggc tccccaaag 
      atcaccaaa gacggcgtc accgtcgcc aaaggcgtg 
      gaactcaaa gacaagttc cagaacatc ggcgcccgc 
      ttagtacag gacgttgcc aacaacacc aacgaggag 
      gcaggggac ggaaccact actgctaca gtattggcc 
      cgctctatt gctaaagag ggtttcgaa aatcttggc 
      aagggcgct aatccagtc gaaattcgc aaaggcata 
      atgctagcg gttgagaag atcacggaa acgttgaaa 
      actctctca aagcccgtc accacacct gaggagata 
      tgccaggtg gccacgatt tcggctaat ggggatcag 
      tcggttggt aatttaatt gccgatgcc atgaagaag 
      gtgggcaaa gaaggcgtg attacggtc aaagatggg 
      aaaactctg cacgatgag cttgaagtt attgaaggg 
      ttcaagttt gaccgcgga tacatttcc ccatacttt 
      gtcaatacc tcaaaaggt gcaaaagtg gaataccag 
      gatgctcta atcctcctc agcgaaaag aaaatctca 
      tcggttcaa agcattgtc cccgccttg gagctggca 
      aacatgcaa aagaaaccc ttaattatc gttgctgaa 
      gacatagac ggcgaagct ttgaccaca ctagtcgtc 
      aacagactg cgtattggt cttcaagtg gcggctgta 
      aaagcccca ggttttggc gacaaccgc aaagccacc 
      ctccaagat atggccatc gccactgga ggtatcgtt 
      tttggagac gaagccaat attgtcaaa cttgaagac 
      gtccaactg tccgatctc ggccaagtt ggcgaaatt 
      gtcatcact aaggatgat acacttatc ttgaaaggg 
      aaaggcaaa aaagacgat atctctaaa cgcgctgag 
      caaattaaa gatcagatt gaaaacacc acttctgaa 
      tacgagaag gagaaactt caggaacgt ttggcgaga 
      ttggcttca ggagtggct ttgttgaaa gtgggaggc 
      agtagcgag gttgaagtt aacgagaaa aaagacaga 
      gtgactgat gctttgaat gcgactaga gcagctgtt 
      gaggaagga attgttcct ggaggtggt actgccttg 
      cttaggtgc tccggcagt ttggatggg ctcaagccg 
      ggaaacaat gaccaagct attggaatc gaaattgtg 
      aagagagct ttgaaagta ccttgcatg accatcgcc 
      aagaacgct ggcgtagat ggcgcgact gttgttgcc 
      aaaatcgag caacaacaa ggtgactac ggttacgat 
      gctcttaac aacgaatat gtcaatatg tttgaaaga 
      ggaatcatt gatcctacg aaggttgtg cgcacagcc 
      cttattgac gcgtctggt gtagcgtca cttttgacc 
      accgccgag gcagtaatc accgaaata ccaaaggaa 
      gaaccgcca attccaagc ggtggaatg ggtggtatg 
      ggaggtatg ggtggaatg ggcggaatg atgtaa
    mRNA sequence
    • >TC013683 RA
      ctgacatct cttttctag aatttctag aaacgtgat 
      cgcaggcgg acaagccgg caaataatt tttattttc 
      taagtttgt gctgttgtt agaccattc aagatgtat 
      cgtttaccg tcgaccatg cgatcagtc gccctcagc 
      aaagccaac agattatcc caaatccag cgttggtac 
      gccaaagac gtccgattc ggccccgaa gtccgggcc 
      ctgatgctc caaggcgtc gacgttctt gcagacgct 
      gtggcggtc accatgggc cctaagggc cgtaacgtg 
      atcatcgaa cagtcgtgg ggctcccca aagatcacc 
      aaagacggc gtcaccgtc gccaaaggc gtggaactc 
      aaagacaag ttccagaac atcggcgcc cgcttagta 
      caggacgtt gccaacaac accaacgag gaggcaggg 
      gacggaacc actactgct acagtattg gcccgctct 
      attgctaaa gagggtttc gaaaatctt ggcaagggc 
      gctaatcca gtcgaaatt cgcaaaggc ataatgcta 
      gcggttgag aagatcacg gaaacgttg aaaactctc 
      tcaaagccc gtcaccaca cctgaggag atatgccag 
      gtggccacg atttcggct aatggggat cagtcggtt 
      ggtaattta attgccgat gccatgaag aaggtgggc 
      aaagaaggc gtgattacg gtcaaagat gggaaaact 
      ctgcacgat gagcttgaa gttattgaa gggttcaag 
      tttgaccgc ggatacatt tccccatac tttgtcaat 
      acctcaaaa ggtgcaaaa gtggaatac caggatgct 
      ctaatcctc ctcagcgaa aagaaaatc tcatcggtt 
      caaagcatt gtccccgcc ttggagctg gcaaacatg 
      caaaagaaa cccttaatt atcgttgct gaagacata 
      gacggcgaa gctttgacc acactagtc gtcaacaga 
      ctgcgtatt ggtcttcaa gtggcggct gtaaaagcc 
      ccaggtttt ggcgacaac cgcaaagcc accctccaa 
      gatatggcc atcgccact ggaggtatc gtttttgga 
      gacgaagcc aatattgtc aaacttgaa gacgtccaa 
      ctgtccgat ctcggccaa gttggcgaa attgtcatc 
      actaaggat gatacactt atcttgaaa gggaaaggc 
      aaaaaagac gatatctct aaacgcgct gagcaaatt 
      aaagatcag attgaaaac accacttct gaatacgag 
      aaggagaaa cttcaggaa cgtttggcg agattggct 
      tcaggagtg gctttgttg aaagtggga ggcagtagc 
      gaggttgaa gttaacgag aaaaaagac agagtgact 
      gatgctttg aatgcgact agagcagct gttgaggaa 
      ggaattgtt cctggaggt ggtactgcc ttgcttagg 
      tgctccggc agtttggat gggctcaag ccgggaaac 
      aatgaccaa gctattgga atcgaaatt gtgaagaga 
      gctttgaaa gtaccttgc atgaccatc gccaagaac 
      gctggcgta gatggcgcg actgttgtt gccaaaatc 
      gagcaacaa caaggtgac tacggttac gatgctctt 
      aacaacgaa tatgtcaat atgtttgaa agaggaatc 
      attgatcct acgaaggtt gtgcgcaca gcccttatt 
      gacgcgtct ggtgtagcg tcacttttg accaccgcc 
      gaggcagta atcaccgaa ataccaaag gaagaaccg 
      ccaattcca agcggtgga atgggtggt atgggaggt 
      atgggtgga atgggcgga atgatgtaa acgcgcgca 
      ccaaacact taatcatga atccatcac tactaggaa 
      aagacaatt gctagttaa gttttagtt cagtgcatc 
      tttccaagt atgtgaatt caatagcgg ccttcgcgt 
      caaagactt ccactatat tttgaccga atgtcgaaa 
      acttatatt aagtttgtg tgattgtta gatgtgttt 
      atttttttc aactcccgg ttttgatgg ttagtgaag 
      agttgaaaa ttcaacgtc tgcgatgtt cgtgttagg 
      tttttgtta cttttataa cgcgacttg aatgaatga 
      aataaattg tgatgtctt gt