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Gene information
General information
TC013550 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8153615..8155577
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013550
    iBeetle screen iB_02156

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48 adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013550 RA
      MDVKTAPTE EELNKLLKK TFTSNLRPN YITVQGFVL 
      PERYREFEK ILKEYEVFD TDVWICGFP KTGTTWISE 
      IAWLIANDL DYEGAKADD YRRTRMLEF SMLFSETFL 
      GGEPYELDS VGFSKDQKH PRSIKSHLP FPLLPEQIL 
      NGTKKPRII CTARNPMDT CVSYYHQCA NYEGFTGTF 
      EEFCKLFLF DKINYGPYW KHVLSFWEH RSKSNILFL 
      TYEEMKKDL PGVLQKVAK LLGKTLSKE DSVRLQQHV 
      SFDSMKKNP AVNKESINN FLVSSGTEV KAPFIRAGK 
      VGGYKNSMS PELIAQFRY WMQKRFEGT GLNLLEE
    CDS sequence
    • >TC013550 RA
      atggacgtt aaaactgct cccaccgaa gaagaactt 
      aataagttg ttgaaaaaa acatttaca agtaatctc 
      agaccgaac tatataacg gtacagggt ttcgtgtta 
      ccagaacga taccgcgaa tttgaaaaa atcctcaaa 
      gagtatgaa gtttttgat acagatgtg tggatttgt 
      gggtttcct aaaactgga acaacctgg atctctgaa 
      attgcttgg ttgattgca aacgatttg gattatgaa 
      ggagccaaa gcggatgat tacaggaga actaggatg 
      ctagaattt agcatgttg ttttctgag acatttctt 
      gggggtgaa ccctacgag ctcgattct gtaggtttc 
      agtaaagat caaaaacac ccaagatct attaaatcg 
      catttaccg tttcccctt ttgccagaa cagattttg 
      aacggaaca aagaaacca agaattatt tgtactgcc 
      agaaaccca atggacacg tgtgtgtct tattaccac 
      caatgtgcc aattatgaa ggcttcact ggcacattt 
      gaagagttt tgtaaacta tttctcttt gataaaatt 
      aattatggt ccttactgg aaacatgtt ttgtcattc 
      tgggagcac agaagcaaa tcaaacatt ttattccta 
      acatacgaa gaaatgaag aaagacttg cctggagtt 
      ttgcaaaaa gtggccaaa ctgcttggc aaaaccttg 
      tcaaaggaa gatagcgtt cgtttacaa cagcatgta 
      tcatttgac tccatgaaa aaaaaccca gctgtgaac 
      aaggaaagt ataaataat ttccttgta tcctctgga 
      accgaagtg aaagcacca tttattaga gctgggaaa 
      gtcgggggt tacaaaaac tcaatgtca ccagagctt 
      attgcacaa tttcgctat tggatgcaa aaaaggttc 
      gaaggcact gggcttaat ctcttggaa gaataa
    mRNA sequence
    • >TC013550 RA
      gtattcaca tacattaaa gttatatta ctgaatcta 
      tacatacat ttgcttaaa atttcatcg gcagtaaac 
      actgtttat acaaatatt gaccgtaga acctgagtt 
      gtctagaga ttcttccaa caatggacg ttaaaactg 
      ctcccaccg aagaagaac ttaataagt tgttgaaaa 
      aaacattta caagtaatc tcagaccga actatataa 
      cggtacagg gtttcgtgt taccagaac gataccgcg 
      aatttgaaa aaatcctca aagagtatg aagtttttg 
      atacagatg tgtggattt gtgggtttc ctaaaactg 
      gaacaacct ggatctctg aaattgctt ggttgattg 
      caaacgatt tggattatg aaggagcca aagcggatg 
      attacagga gaactagga tgctagaat ttagcatgt 
      tgttttctg agacatttc ttgggggtg aaccctacg 
      agctcgatt ctgtaggtt tcagtaaag atcaaaaac 
      acccaagat ctattaaat cgcatttac cgtttcccc 
      ttttgccag aacagattt tgaacggaa caaagaaac 
      caagaatta tttgtactg ccagaaacc caatggaca 
      cgtgtgtgt cttattacc accaatgtg ccaattatg 
      aaggcttca ctggcacat ttgaagagt tttgtaaac 
      tatttctct ttgataaaa ttaattatg gtccttact 
      ggaaacatg ttttgtcat tctgggagc acagaagca 
      aatcaaaca ttttattcc taacatacg aagaaatga 
      agaaagact tgcctggag ttttgcaaa aagtggcca 
      aactgcttg gcaaaacct tgtcaaagg aagatagcg 
      ttcgtttac aacagcatg tatcatttg actccatga 
      aaaaaaacc cagctgtga acaaggaaa gtataaata 
      atttccttg tatcctctg gaaccgaag tgaaagcac 
      catttatta gagctggga aagtcgggg gttacaaaa 
      actcaatgt caccagagc ttattgcac aatttcgct 
      attggatgc aaaaaaggt tcgaaggca ctgggctta 
      atctcttgg aagaataac ataaaaacc aaaaatttt 
      gtttttgta tttgtagat acaagtagg ggtaaactg 
      gggaatcgt tttatttta attgttgca tgatttaat 
      aaaatattt tgggtatat ttttgtgtt tctttaaca 
      cccatagaa cctggcaca gctttgctc acttgtgag 
      ctctttcgt tgtcacgtt aacagtata acaagtgaa 
      atttttaat aaacaaaac taatgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY