Status
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Gene information
General information
TC013369 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10417531..10420447
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013369
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02120

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013369 RA
      MATSLKRNR SRRRIAAVT FLSNISLDG SYRDTRLSL 
      LPRNGAIIK TTGLLAATD EPLVEESDG PDDCFSDTD 
      HSKEKIPNP KKKFQKSKH YNVDGHSLS SDSESVTPI 
      KSHLEEAAK SIKDGISER KIIPTFRKR IPHQGSLGS 
      DTEKHHYGS SSESLGPSI SRCKTSPAP PSIPEATKE 
      IKIIKAAKT HRFKDERIV MVTSRYIPF MVCSIIPYK 
      KSHRNEAKR ESGRKRTTS GTRPLSAAA DGLDPFDSL 
      GIEKAPDGQ EFSYGYLLV PTKPVKEFR EKRLNTIDD 
      PTEPGHVSF NVTKRPHHV FARTKVLTW QPDYKWSFS 
      YDQASIRAN THVVAASPP PDAKDETSL NSLVYHPNL 
      LDDPELIAG KHRTLLTFT SYMTSVIDY VRPSDLKKE 
      INDKFREKF PHIQLTLSK LRSIKREMR KIAKTECNI 
      DLVTVAQAY VYFEKLILR GLINKQNRK LCAGASLIL 
      SAKLNDVKG DGLKTLLEK TENTFRLNR KELVAAEFA 
      VLVALEFGL HIPTWEIFP HYQRLLYES
    CDS sequence
    • >TC013369 RA
      atggcaact tcattaaaa agaaaccgt tcgcgacgt 
      cgcattgct gctgttact ttcttatca aatatttcc 
      ctggacggc agttacaga gacaccaga ttatcgtta 
      cttccccgt aacggggct atcatcaaa accaccggt 
      ctcctagcg gcgaccgat gaacccctc gtcgaagaa 
      tccgacggt ccggacgac tgcttcagc gacacggac 
      cactcgaaa gagaaaatc cccaatccg aaaaagaaa 
      ttccaaaaa tcgaaacat tacaacgtc gacggacac 
      tcgctgagt tccgactcg gagagtgtc acgccgatc 
      aaatcccac ctcgaggaa gccgccaaa agcatcaag 
      gacggcatt tccgagcgc aaaatcatc cccacgttt 
      cgcaaacgc atcccgcat cagggctcg ttggggagc 
      gacacagag aagcaccac tatggcagc agttccgag 
      agtctgggc ccctcaatt tcgcggtgc aagacaagt 
      ccagcccca ccctcgatc ccggaggcg acgaaagaa 
      attaagatc atcaaagcg gcgaaaacg caccgtttt 
      aaagacgaa agaatcgtg atggtgact tcgcggtac 
      ataccattc atggtgtgc tcgataata ccgtacaag 
      aagagtcac aggaacgag gcgaaacgg gaaagtggg 
      cggaaaagg acgacttcg gggacgcgg cccttgtcg 
      gcggccgcc gacggcttg gaccccttc gattctttg 
      gggatcgag aaagccccc gatggacag gaattttcg 
      tacgggtac ttgttggta ccaacgaaa ccggtgaaa 
      gagtttaga gagaaaagg ttgaacact attgacgac 
      ccgacggag cccgggcat gtctcattt aacgttacg 
      aaacgtccg caccatgtt ttcgcccgg acaaaagtt 
      ttaacgtgg caacctgac tacaaatgg tcgttttcg 
      tacgatcag gcttcaatt cgagccaat acgcacgtg 
      gtagccgca agtccgccc ccggacgct aaggacgaa 
      acgtcgtta aactcccta gtctatcac ccgaattta 
      ctagacgac ccggaattg atcgccggg aaacaccgg 
      actttacta actttcacg tcttatatg acgtcagtt 
      atcgattat gtgcgacct tctgacttg aagaaggaa 
      atcaacgac aagtttcgg gagaaattc ccgcacatt 
      cagctcact ttgagcaaa ttacgaagt atcaagcgc 
      gaaatgcgt aaaatcgca aaaaccgaa tgtaacatt 
      gatctggtg accgtagct caagcgtac gtctatttt 
      gaaaaattg atattacgt ggactgatt aataaacaa 
      aacagaaag ttgtgtgct ggcgcaagt ttgatattg 
      tcggcgaaa ctcaacgac gttaaagga gacggcttg 
      aagacgctt cttgaaaag acagaaaac acattccga 
      ctgaacagg aaggaactt gtggcagct gaattcgct 
      gttttggta gcactggaa ttcgggctt catatacca 
      acgtgggag atcttccct cactatcag agattgtta 
      tatgaatct tga
    mRNA sequence
    • >TC013369 RA
      aataaatac tcgagataa acgagttga gagcgtatt 
      taaagaaca atttgcggc ccaatttta aaattctag 
      tgtttttgc tttgtgaat tttggcagt gatgtctca 
      tgtgtcagt tgtcactgc gacgtaaat attttcctt 
      gtaggtctg tgtgttagt ggacttttt ttaagcaaa 
      aagtgtgtg aaatagtgt acgaaaatg tgcaagtcg 
      ctgatatta ttcctgttg aacagtatc gagtttcat 
      atatgcgct tttcatcgc aaatcatta acaatgtgc 
      aataaaagg actatgtca ataatggat cttcagtga 
      cataacact ccacttccc cccatacac aactactaa 
      aaaagcaat aaaaaatct aatggcaac ttcattaaa 
      aagaaaccg ttcgcgacg tcgcattgc tgctgttac 
      tttcttatc aaatatttc cctggacgg cagttacag 
      agacaccag attatcgtt acttccccg taacggggc 
      tatcatcaa aaccaccgg tctcctagc ggcgaccga 
      tgaacccct cgtcgaaga atccgacgg tccggacga 
      ctgcttcag cgacacgga ccactcgaa agagaaaat 
      ccccaatcc gaaaaagaa attccaaaa atcgaaaca 
      ttacaacgt cgacggaca ctcgctgag ttccgactc 
      ggagagtgt cacgccgat caaatccca cctcgagga 
      agccgccaa aagcatcaa ggacggcat ttccgagcg 
      caaaatcat ccccacgtt tcgcaaacg catcccgca 
      tcagggctc gttggggag cgacacaga gaagcacca 
      ctatggcag cagttccga gagtctggg cccctcaat 
      ttcgcggtg caagacaag tccagcccc accctcgat 
      cccggaggc gacgaaaga aattaagat catcaaagc 
      ggcgaaaac gcaccgttt taaagacga aagaatcgt 
      gatggtgac ttcgcggta cataccatt catggtgtg 
      ctcgataat accgtacaa gaagagtca caggaacga 
      ggcgaaacg ggaaagtgg gcggaaaag gacgacttc 
      ggggacgcg gcccttgtc ggcggccgc cgacggctt 
      ggacccctt cgattcttt ggggatcga gaaagcccc 
      cgatggaca ggaattttc gtacgggta cttgttggt 
      accaacgaa accggtgaa agagtttag agagaaaag 
      gttgaacac tattgacga cccgacgga gcccgggca 
      tgtctcatt taacgttac gaaacgtcc gcaccatgt 
      tttcgcccg gacaaaagt tttaacgtg gcaacctga 
      ctacaaatg gtcgttttc gtacgatca ggcttcaat 
      tcgagccaa tacgcacgt ggtagccgc aagtccgcc 
      cccggacgc taaggacga aacgtcgtt aaactccct 
      agtctatca cccgaattt actagacga cccggaatt 
      gatcgccgg gaaacaccg gactttact aactttcac 
      gtcttatat gacgtcagt tatcgatta tgtgcgacc 
      ttctgactt gaagaagga aatcaacga caagtttcg 
      ggagaaatt cccgcacat tcagctcac tttgagcaa 
      attacgaag tatcaagcg cgaaatgcg taaaatcgc 
      aaaaaccga atgtaacat tgatctggt gaccgtagc 
      tcaagcgta cgtctattt tgaaaaatt gatattacg 
      tggactgat taataaaca aaacagaaa gttgtgtgc 
      tggcgcaag tttgatatt gtcggcgaa actcaacga 
      cgttaaagg agacggctt gaagacgct tcttgaaaa 
      gacagaaaa cacattccg actgaacag gaaggaact 
      tgtggcagc tgaattcgc tgttttggt agcactgga 
      attcgggct tcatatacc aacgtggga gatcttccc 
      tcactatca gagattgtt atatgaatc ttgaccgat 
      gctttttaa tgtcttgca tgctcaaca tattttcgt 
      ttttaataa tcttgccat ttaaagtac aaattgtca 
      atctcctgt acttaattt ctctgacga cgaactact 
      gatattatc tagggtaga ttgtagtac ttttgtatg 
      agactttga cgagggttt gaaaggtgg ctgtgatgc 
      atattgtgg aatttttgg ttagttagg taccacctt 
      tcttactga aggtcctta aaggccagg tgatataaa 
      aaggtactt gaatttagc atacttaat tttttctat 
      agtcggtgt gttccttgt tagattaag aaaactttg 
      ttttttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY