General information | |||
TC031159
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 14733088..14737115 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031159 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02051 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax dorsal partiallynot fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
urogomphi mostly present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031159 RA
MYLSWFIVI MFLKGATMT LTTRRVVES VELGEGPHW DPETQSLYF VDIYGKAIH KYVPATKKH TKAVIGINH VSLIVPVQG QKNKFLISI GRDLNIVTW DGESETVSG MEKIYEIDN TPDTLQNRF NDGKCDALG RLWAGSMGA QPVPGHVEL NKGSFISLE SGRKAKTHL TKVGISNGL AWSLDNKYL YYIDSQKNT LDRYDFNLE KGTISNGES IFSLKKANL SGILDGMTI DTDGNLWIA NFGGGRVLK VDPRKPQTL LQIIQMPAH QVTSVAFGG PNLDELYVT TARLTTDGV AYPPPDNGA TYVITGTEA KGLPGIGFK LEPSRYFRV RLSKRLNCF NSISLVNMA PTIERIVDS VELGEGPHW DAATQSLYF VDIFGKTIH KYVPVTKKH TKAVIGTNH VSLIIPVEG EKNKFLITI GRQLVTVKW DGSSEKVSE ITKIGEVDD DPETLDNRF NDGKCDPTG RLWAGTMGG EPINGHVKP NKGGLFSLG PNQQIRKHL SNVSISNGL AWSKDLKKM YYIDSPKRT IDEYEVDMK YGTLSNGRP IFTLEKHNI QGFPDGMAI DTDGNLWVA VFNGYRVIK IDPRKPETL LQTIQLPAK QVTSVAFGG PDLDELYVT SAAFTVDGV ELPPPDHGA TFRIKGIGA KGYPGVNVK LQLNNTMVR ITQIGDNLE VGTRIHWDE QTQNLYYVD VPTSTIYRY RPSTDEITQ AQVGNEPLA FAFPVDGKT DFFIAGLGR KIVLLKWDG ESESVCSCT TIAEVDREP HLAKNRLNG AKVDPYGRL WAGTMGAQD ANGQTIPKQ GSLYSLTNG RLKREFKEV GISNGIAFD LDANKMYYV DTLIPAISA FDYDGESGE ISNKTTVFK LECSCINGL PDGLTIDTD GNLWLAVIF GSLVLKIDP RTGQLLKKI QMPTPQITA MTWGNKNCD VLYVTSAKM AVNGKVPEK PAGCTFKLE NLGHSKGLP GDRYKM
- >TC031159 RA
atgtattta tcctggttt atcgtaata atgtttctg aaaggggca acaatgacc ctaacaaca cgacgggtt gttgagagt gtggaactg ggggaaggc ccgcattgg gaccctgaa acccaaagt ttgtacttt gtggacatt tatggcaaa gctattcac aaatacgtc cccgctacc aaaaaacac acaaaagct gtgatcgga attaatcac gtctcgttg attgttccg gtgcaaggc caaaaaaac aaatttctt attagcatt ggccgtgat ttaaacatc gtgacgtgg gacggggaa agtgaaacg gtctcgggc atggaaaaa atttacgaa attgataac acacccgac acgcttcaa aacagattt aatgacggg aaatgtgac gctttgggg cgtctatgg gccgggtca atgggggca caaccagtc ccgggacac gtggagcta aacaagggc tcatttatc agtttagag tcaggacgc aaagccaaa acacacctc actaaagtt gggatttcg aatggtttg gcttggagt ttggacaac aagtacttg tattacatc gactcacaa aaaaacaca cttgatcga tacgatttt aatctcgag aaggggaca atttccaat ggcgagagc attttttcg ttaaaaaaa gccaacctg agtgggata ttagacgga atgaccatt gatactgac ggcaatttg tggattgct aattttgga ggtgggcgc gtactcaaa gtggacccc agaaagccc caaacctta ctgcagata attcaaatg ccagcgcac caagttact tctgttgcc ttcgggggg cctaatcta gacgaactt tacgtcacc acagcccga cttacgaca gatggagtg gcgtatccg ccccccgat aatggggcc acgtatgtc attacaggg acagaggcc aaaggactc ccagggatc ggatttaaa ttagaaccc agtagatat ttcagggtc aggctatca aagcgcctc aattgcttc aattccata tcattagtg aacatggca ccaaccatc gaacgtatc gtcgacagt gtcgaactg ggcgaaggc cctcactgg gacgccgcc acacaaagc ctctatttc gtcgacatt ttcgggaaa accatccat aaatacgtc cctgtcacg aaaaaacac accaaagcg gtgattgga acaaaccac gtctcgtta ataatcccg gtcgaaggc gaaaaaaac aagtttttg ataacaata ggtcgacaa ttggtgaca gtgaaatgg gacggaagt agcgagaaa gttagcgaa attacaaaa ataggcgaa gttgatgac gacccggaa actctcgat aacaggttc aacgacggg aaatgcgac cccacgggg agattatgg gcggggact atgggaggc gaaccaatc aacggacac gtcaagcca aataaaggc gggcttttc agtttagga cccaatcaa caaataagg aaacatttg agcaatgtc tccatctcc aatggcctg gcgtggagc aaagatttg aaaaaaatg tattacatt gattcgcca aaacggaca atcgatgag tatgaagtg gatatgaag tatggcact ctttcaaac ggaagaccc atttttacg ttagagaaa cacaatatt caaggattc cctgatggg atggctatt gacaccgat ggtaattta tgggtggct gtttttaac ggatatagg gtcattaaa atcgacccc cgaaaacct gaaactctc ctacagacg attcaattg cccgctaaa caagtaact tctgtcgct ttcggaggc cctgattta gacgaactg tacgttacc tccgccgct ttcacggta gatggcgtg gaactaccg cctcccgat catggagca actttcagg attaaagga ataggggca aaaggatac ccgggagtt aatgtaaaa ttacagtta aataacaca atggtccga ataacccaa attggggat aatctagaa gtgggcact cgcatccat tgggacgaa caaacgcaa aatctttat tacgtggac gtcccaaca tcaaccatt tacagatac agaccgtca acggatgaa ataacccaa gcgcaagtt ggaaacgaa cctctagct tttgcattt ccggtcgat ggtaaaacc gactttttt attgctgga cttgggcga aaaatcgtt ttgttaaaa tgggacggt gagagtgaa agtgtttgt tcgtgtact accattgca gaagtcgat agagaacca catttggcc aaaaatcgg ctaaatggc gccaaagtt gacccatat gggcgtttg tgggccggt accatgggc gcacaggac gccaacggg caaacaatc cccaaacag gggtcgctg tatagctta accaacggt agacttaaa cgagagttc aaggaagtt ggtatttcg aatgggatt gcattcgat ttggacgcg aacaaaatg tattatgtt gacacgtta atacctgcc atttcggca ttcgattac gatggcgaa agcggcgaa atcagtaac aaaacaaca gtctttaaa ttagaatgc agttgcata aatggcctt cccgacgga ttgacaatc gacactgac ggtaattta tggttggct gtgattttc ggttcactt gttttaaaa attgaccct cgaaccggc caacttttg aaaaaaatt caaatgcca acgcctcaa atcaccgca atgacttgg ggcaacaaa aactgcgac gtcttgtat gtcactagc gccaaaatg gcagtaaac ggaaaagtg cccgaaaaa ccggccggt tgcactttt aaattggaa aatttgggg cacagtaag ggcttacct ggtgaccgt tataaaatg taa
- >TC031159 RA
ataaaatca acaataatt aaacgataa agtaacaat aatttcggt gctttccgg tgaatatta caacagaaa ccttgcacc taaaaagac aaatttcgt gaatcattt tttttagtt ttaccgaaa tgtatttat cctggttta tcgtaataa tgtttctga aaggggcaa caatgaccc taacaacac gacgggttg ttgagagtg tggaactgg gggaaggcc cgcattggg accctgaaa cccaaagtt tgtactttg tggacattt atggcaaag ctattcaca aatacgtcc ccgctacca aaaaacaca caaaagctg tgatcggaa ttaatcacg tctcgttga ttgttccgg tgcaaggcc aaaaaaaca aatttctta ttagcattg gccgtgatt taaacatcg tgacgtggg acggggaaa gtgaaacgg tctcgggca tggaaaaaa tttacgaaa ttgataaca cacccgaca cgcttcaaa acagattta atgacggga aatgtgacg ctttggggc gtctatggg ccgggtcaa tgggggcac aaccagtcc cgggacacg tggagctaa acaagggct catttatca gtttagagt caggacgca aagccaaaa cacacctca ctaaagttg ggatttcga atggtttgg cttggagtt tggacaaca agtacttgt attacatcg actcacaaa aaaacacac ttgatcgat acgatttta atctcgaga aggggacaa tttccaatg gcgagagca ttttttcgt taaaaaaag ccaacctga gtgggatat tagacggaa tgaccattg atactgacg gcaatttgt ggattgcta attttggag gtgggcgcg tactcaaag tggacccca gaaagcccc aaaccttac tgcagataa ttcaaatgc cagcgcacc aagttactt ctgttgcct tcggggggc ctaatctag acgaacttt acgtcacca cagcccgac ttacgacag atggagtgg cgtatccgc cccccgata atggggcca cgtatgtca ttacaggga cagaggcca aaggactcc cagggatcg gatttaaat tagaaccca gtagatatt tcagggtca ggctatcaa agcgcctca attgcttca attccatat cattagtga acatggcac caaccatcg aacgtatcg tcgacagtg tcgaactgg gcgaaggcc ctcactggg acgccgcca cacaaagcc tctatttcg tcgacattt tcgggaaaa ccatccata aatacgtcc ctgtcacga aaaaacaca ccaaagcgg tgattggaa caaaccacg tctcgttaa taatcccgg tcgaaggcg aaaaaaaca agtttttga taacaatag gtcgacaat tggtgacag tgaaatggg acggaagta gcgagaaag ttagcgaaa ttacaaaaa taggcgaag ttgatgacg acccggaaa ctctcgata acaggttca acgacggga aatgcgacc ccacgggga gattatggg cggggacta tgggaggcg aaccaatca acggacacg tcaagccaa ataaaggcg ggcttttca gtttaggac ccaatcaac aaataagga aacatttga gcaatgtct ccatctcca atggcctgg cgtggagca aagatttga aaaaaatgt attacattg attcgccaa aacggacaa tcgatgagt atgaagtgg atatgaagt atggcactc tttcaaacg gaagaccca tttttacgt tagagaaac acaatattc aaggattcc ctgatggga tggctattg acaccgatg gtaatttat gggtggctg tttttaacg gatataggg tcattaaaa tcgaccccc gaaaacctg aaactctcc tacagacga ttcaattgc ccgctaaac aagtaactt ctgtcgctt tcggaggcc ctgatttag acgaactgt acgttacct ccgccgctt tcacggtag atggcgtgg aactaccgc ctcccgatc atggagcaa ctttcagga ttaaaggaa taggggcaa aaggatacc cgggagtta atgtaaaat tacagttaa ataacacaa tggtccgaa taacccaaa ttggggata atctagaag tgggcactc gcatccatt gggacgaac aaacgcaaa atctttatt acgtggacg tcccaacat caaccattt acagataca gaccgtcaa cggatgaaa taacccaag cgcaagttg gaaacgaac ctctagctt ttgcatttc cggtcgatg gtaaaaccg actttttta ttgctggac ttgggcgaa aaatcgttt tgttaaaat gggacggtg agagtgaaa gtgtttgtt cgtgtacta ccattgcag aagtcgata gagaaccac atttggcca aaaatcggc taaatggcg ccaaagttg acccatatg ggcgtttgt gggccggta ccatgggcg cacaggacg ccaacgggc aaacaatcc ccaaacagg ggtcgctgt atagcttaa ccaacggta gacttaaac gagagttca aggaagttg gtatttcga atgggattg cattcgatt tggacgcga acaaaatgt attatgttg acacgttaa tacctgcca tttcggcat tcgattacg atggcgaaa gcggcgaaa tcagtaaca aaacaacag tctttaaat tagaatgca gttgcataa atggccttc ccgacggat tgacaatcg acactgacg gtaatttat ggttggctg tgattttcg gttcacttg ttttaaaaa ttgaccctc gaaccggcc aacttttga aaaaaattc aaatgccaa cgcctcaaa tcaccgcaa tgacttggg gcaacaaaa actgcgacg tcttgtatg tcactagcg ccaaaatgg cagtaaacg gaaaagtgc ccgaaaaac cggccggtt gcactttta aattggaaa atttggggc acagtaagg gcttacctg gtgaccgtt ataaaatgt aaccttaaa tagcgcttt attgtacat attta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
