Status
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Gene information
General information
TC031159
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013051,TC013049,TC013050
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14733088..14737115
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031159
    iBeetle screen iB_02051

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax dorsal partiallynot fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi mostly present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031159 RA
      MYLSWFIVI MFLKGATMT LTTRRVVES VELGEGPHW 
      DPETQSLYF VDIYGKAIH KYVPATKKH TKAVIGINH 
      VSLIVPVQG QKNKFLISI GRDLNIVTW DGESETVSG 
      MEKIYEIDN TPDTLQNRF NDGKCDALG RLWAGSMGA 
      QPVPGHVEL NKGSFISLE SGRKAKTHL TKVGISNGL 
      AWSLDNKYL YYIDSQKNT LDRYDFNLE KGTISNGES 
      IFSLKKANL SGILDGMTI DTDGNLWIA NFGGGRVLK 
      VDPRKPQTL LQIIQMPAH QVTSVAFGG PNLDELYVT 
      TARLTTDGV AYPPPDNGA TYVITGTEA KGLPGIGFK 
      LEPSRYFRV RLSKRLNCF NSISLVNMA PTIERIVDS 
      VELGEGPHW DAATQSLYF VDIFGKTIH KYVPVTKKH 
      TKAVIGTNH VSLIIPVEG EKNKFLITI GRQLVTVKW 
      DGSSEKVSE ITKIGEVDD DPETLDNRF NDGKCDPTG 
      RLWAGTMGG EPINGHVKP NKGGLFSLG PNQQIRKHL 
      SNVSISNGL AWSKDLKKM YYIDSPKRT IDEYEVDMK 
      YGTLSNGRP IFTLEKHNI QGFPDGMAI DTDGNLWVA 
      VFNGYRVIK IDPRKPETL LQTIQLPAK QVTSVAFGG 
      PDLDELYVT SAAFTVDGV ELPPPDHGA TFRIKGIGA 
      KGYPGVNVK LQLNNTMVR ITQIGDNLE VGTRIHWDE 
      QTQNLYYVD VPTSTIYRY RPSTDEITQ AQVGNEPLA 
      FAFPVDGKT DFFIAGLGR KIVLLKWDG ESESVCSCT 
      TIAEVDREP HLAKNRLNG AKVDPYGRL WAGTMGAQD 
      ANGQTIPKQ GSLYSLTNG RLKREFKEV GISNGIAFD 
      LDANKMYYV DTLIPAISA FDYDGESGE ISNKTTVFK 
      LECSCINGL PDGLTIDTD GNLWLAVIF GSLVLKIDP 
      RTGQLLKKI QMPTPQITA MTWGNKNCD VLYVTSAKM 
      AVNGKVPEK PAGCTFKLE NLGHSKGLP GDRYKM
    CDS sequence
    • >TC031159 RA
      atgtattta tcctggttt atcgtaata atgtttctg 
      aaaggggca acaatgacc ctaacaaca cgacgggtt 
      gttgagagt gtggaactg ggggaaggc ccgcattgg 
      gaccctgaa acccaaagt ttgtacttt gtggacatt 
      tatggcaaa gctattcac aaatacgtc cccgctacc 
      aaaaaacac acaaaagct gtgatcgga attaatcac 
      gtctcgttg attgttccg gtgcaaggc caaaaaaac 
      aaatttctt attagcatt ggccgtgat ttaaacatc 
      gtgacgtgg gacggggaa agtgaaacg gtctcgggc 
      atggaaaaa atttacgaa attgataac acacccgac 
      acgcttcaa aacagattt aatgacggg aaatgtgac 
      gctttgggg cgtctatgg gccgggtca atgggggca 
      caaccagtc ccgggacac gtggagcta aacaagggc 
      tcatttatc agtttagag tcaggacgc aaagccaaa 
      acacacctc actaaagtt gggatttcg aatggtttg 
      gcttggagt ttggacaac aagtacttg tattacatc 
      gactcacaa aaaaacaca cttgatcga tacgatttt 
      aatctcgag aaggggaca atttccaat ggcgagagc 
      attttttcg ttaaaaaaa gccaacctg agtgggata 
      ttagacgga atgaccatt gatactgac ggcaatttg 
      tggattgct aattttgga ggtgggcgc gtactcaaa 
      gtggacccc agaaagccc caaacctta ctgcagata 
      attcaaatg ccagcgcac caagttact tctgttgcc 
      ttcgggggg cctaatcta gacgaactt tacgtcacc 
      acagcccga cttacgaca gatggagtg gcgtatccg 
      ccccccgat aatggggcc acgtatgtc attacaggg 
      acagaggcc aaaggactc ccagggatc ggatttaaa 
      ttagaaccc agtagatat ttcagggtc aggctatca 
      aagcgcctc aattgcttc aattccata tcattagtg 
      aacatggca ccaaccatc gaacgtatc gtcgacagt 
      gtcgaactg ggcgaaggc cctcactgg gacgccgcc 
      acacaaagc ctctatttc gtcgacatt ttcgggaaa 
      accatccat aaatacgtc cctgtcacg aaaaaacac 
      accaaagcg gtgattgga acaaaccac gtctcgtta 
      ataatcccg gtcgaaggc gaaaaaaac aagtttttg 
      ataacaata ggtcgacaa ttggtgaca gtgaaatgg 
      gacggaagt agcgagaaa gttagcgaa attacaaaa 
      ataggcgaa gttgatgac gacccggaa actctcgat 
      aacaggttc aacgacggg aaatgcgac cccacgggg 
      agattatgg gcggggact atgggaggc gaaccaatc 
      aacggacac gtcaagcca aataaaggc gggcttttc 
      agtttagga cccaatcaa caaataagg aaacatttg 
      agcaatgtc tccatctcc aatggcctg gcgtggagc 
      aaagatttg aaaaaaatg tattacatt gattcgcca 
      aaacggaca atcgatgag tatgaagtg gatatgaag 
      tatggcact ctttcaaac ggaagaccc atttttacg 
      ttagagaaa cacaatatt caaggattc cctgatggg 
      atggctatt gacaccgat ggtaattta tgggtggct 
      gtttttaac ggatatagg gtcattaaa atcgacccc 
      cgaaaacct gaaactctc ctacagacg attcaattg 
      cccgctaaa caagtaact tctgtcgct ttcggaggc 
      cctgattta gacgaactg tacgttacc tccgccgct 
      ttcacggta gatggcgtg gaactaccg cctcccgat 
      catggagca actttcagg attaaagga ataggggca 
      aaaggatac ccgggagtt aatgtaaaa ttacagtta 
      aataacaca atggtccga ataacccaa attggggat 
      aatctagaa gtgggcact cgcatccat tgggacgaa 
      caaacgcaa aatctttat tacgtggac gtcccaaca 
      tcaaccatt tacagatac agaccgtca acggatgaa 
      ataacccaa gcgcaagtt ggaaacgaa cctctagct 
      tttgcattt ccggtcgat ggtaaaacc gactttttt 
      attgctgga cttgggcga aaaatcgtt ttgttaaaa 
      tgggacggt gagagtgaa agtgtttgt tcgtgtact 
      accattgca gaagtcgat agagaacca catttggcc 
      aaaaatcgg ctaaatggc gccaaagtt gacccatat 
      gggcgtttg tgggccggt accatgggc gcacaggac 
      gccaacggg caaacaatc cccaaacag gggtcgctg 
      tatagctta accaacggt agacttaaa cgagagttc 
      aaggaagtt ggtatttcg aatgggatt gcattcgat 
      ttggacgcg aacaaaatg tattatgtt gacacgtta 
      atacctgcc atttcggca ttcgattac gatggcgaa 
      agcggcgaa atcagtaac aaaacaaca gtctttaaa 
      ttagaatgc agttgcata aatggcctt cccgacgga 
      ttgacaatc gacactgac ggtaattta tggttggct 
      gtgattttc ggttcactt gttttaaaa attgaccct 
      cgaaccggc caacttttg aaaaaaatt caaatgcca 
      acgcctcaa atcaccgca atgacttgg ggcaacaaa 
      aactgcgac gtcttgtat gtcactagc gccaaaatg 
      gcagtaaac ggaaaagtg cccgaaaaa ccggccggt 
      tgcactttt aaattggaa aatttgggg cacagtaag 
      ggcttacct ggtgaccgt tataaaatg taa
    mRNA sequence
    • >TC031159 RA
      ataaaatca acaataatt aaacgataa agtaacaat 
      aatttcggt gctttccgg tgaatatta caacagaaa 
      ccttgcacc taaaaagac aaatttcgt gaatcattt 
      tttttagtt ttaccgaaa tgtatttat cctggttta 
      tcgtaataa tgtttctga aaggggcaa caatgaccc 
      taacaacac gacgggttg ttgagagtg tggaactgg 
      gggaaggcc cgcattggg accctgaaa cccaaagtt 
      tgtactttg tggacattt atggcaaag ctattcaca 
      aatacgtcc ccgctacca aaaaacaca caaaagctg 
      tgatcggaa ttaatcacg tctcgttga ttgttccgg 
      tgcaaggcc aaaaaaaca aatttctta ttagcattg 
      gccgtgatt taaacatcg tgacgtggg acggggaaa 
      gtgaaacgg tctcgggca tggaaaaaa tttacgaaa 
      ttgataaca cacccgaca cgcttcaaa acagattta 
      atgacggga aatgtgacg ctttggggc gtctatggg 
      ccgggtcaa tgggggcac aaccagtcc cgggacacg 
      tggagctaa acaagggct catttatca gtttagagt 
      caggacgca aagccaaaa cacacctca ctaaagttg 
      ggatttcga atggtttgg cttggagtt tggacaaca 
      agtacttgt attacatcg actcacaaa aaaacacac 
      ttgatcgat acgatttta atctcgaga aggggacaa 
      tttccaatg gcgagagca ttttttcgt taaaaaaag 
      ccaacctga gtgggatat tagacggaa tgaccattg 
      atactgacg gcaatttgt ggattgcta attttggag 
      gtgggcgcg tactcaaag tggacccca gaaagcccc 
      aaaccttac tgcagataa ttcaaatgc cagcgcacc 
      aagttactt ctgttgcct tcggggggc ctaatctag 
      acgaacttt acgtcacca cagcccgac ttacgacag 
      atggagtgg cgtatccgc cccccgata atggggcca 
      cgtatgtca ttacaggga cagaggcca aaggactcc 
      cagggatcg gatttaaat tagaaccca gtagatatt 
      tcagggtca ggctatcaa agcgcctca attgcttca 
      attccatat cattagtga acatggcac caaccatcg 
      aacgtatcg tcgacagtg tcgaactgg gcgaaggcc 
      ctcactggg acgccgcca cacaaagcc tctatttcg 
      tcgacattt tcgggaaaa ccatccata aatacgtcc 
      ctgtcacga aaaaacaca ccaaagcgg tgattggaa 
      caaaccacg tctcgttaa taatcccgg tcgaaggcg 
      aaaaaaaca agtttttga taacaatag gtcgacaat 
      tggtgacag tgaaatggg acggaagta gcgagaaag 
      ttagcgaaa ttacaaaaa taggcgaag ttgatgacg 
      acccggaaa ctctcgata acaggttca acgacggga 
      aatgcgacc ccacgggga gattatggg cggggacta 
      tgggaggcg aaccaatca acggacacg tcaagccaa 
      ataaaggcg ggcttttca gtttaggac ccaatcaac 
      aaataagga aacatttga gcaatgtct ccatctcca 
      atggcctgg cgtggagca aagatttga aaaaaatgt 
      attacattg attcgccaa aacggacaa tcgatgagt 
      atgaagtgg atatgaagt atggcactc tttcaaacg 
      gaagaccca tttttacgt tagagaaac acaatattc 
      aaggattcc ctgatggga tggctattg acaccgatg 
      gtaatttat gggtggctg tttttaacg gatataggg 
      tcattaaaa tcgaccccc gaaaacctg aaactctcc 
      tacagacga ttcaattgc ccgctaaac aagtaactt 
      ctgtcgctt tcggaggcc ctgatttag acgaactgt 
      acgttacct ccgccgctt tcacggtag atggcgtgg 
      aactaccgc ctcccgatc atggagcaa ctttcagga 
      ttaaaggaa taggggcaa aaggatacc cgggagtta 
      atgtaaaat tacagttaa ataacacaa tggtccgaa 
      taacccaaa ttggggata atctagaag tgggcactc 
      gcatccatt gggacgaac aaacgcaaa atctttatt 
      acgtggacg tcccaacat caaccattt acagataca 
      gaccgtcaa cggatgaaa taacccaag cgcaagttg 
      gaaacgaac ctctagctt ttgcatttc cggtcgatg 
      gtaaaaccg actttttta ttgctggac ttgggcgaa 
      aaatcgttt tgttaaaat gggacggtg agagtgaaa 
      gtgtttgtt cgtgtacta ccattgcag aagtcgata 
      gagaaccac atttggcca aaaatcggc taaatggcg 
      ccaaagttg acccatatg ggcgtttgt gggccggta 
      ccatgggcg cacaggacg ccaacgggc aaacaatcc 
      ccaaacagg ggtcgctgt atagcttaa ccaacggta 
      gacttaaac gagagttca aggaagttg gtatttcga 
      atgggattg cattcgatt tggacgcga acaaaatgt 
      attatgttg acacgttaa tacctgcca tttcggcat 
      tcgattacg atggcgaaa gcggcgaaa tcagtaaca 
      aaacaacag tctttaaat tagaatgca gttgcataa 
      atggccttc ccgacggat tgacaatcg acactgacg 
      gtaatttat ggttggctg tgattttcg gttcacttg 
      ttttaaaaa ttgaccctc gaaccggcc aacttttga 
      aaaaaattc aaatgccaa cgcctcaaa tcaccgcaa 
      tgacttggg gcaacaaaa actgcgacg tcttgtatg 
      tcactagcg ccaaaatgg cagtaaacg gaaaagtgc 
      ccgaaaaac cggccggtt gcactttta aattggaaa 
      atttggggc acagtaagg gcttacctg gtgaccgtt 
      ataaaatgt aaccttaaa tagcgcttt attgtacat 
      attta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY