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Gene information
General information
TC012869 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15369014..15371287
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012869
    iBeetle screen iB_02024

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012869 RA
      MALADDQQF CLRWNNFQA NITSQFEAL RDDEDFTDV 
      TIACEGQRM QAHKVVLSA CSPFFKELF KTNPCSHPI 
      IFMRDVEAR HIVALMEFM YAGEVNVAQ AHLSAFLKT 
      AESLKIRGL TDTSAESEQ KDEDTLYLN PQPSKPGKH 
      KVSSSSTVT SSQDVSNTS VSSPPSKRQ CKSEPEVPR 
      VKIKEDAIN PFALQEPRN QDLVQPKME LPDYLSDDD 
      GRESHFYAG DVTELPGGM EIIPQVAGF VKEQPQESA 
      PDTRKLHSL DPRPCEECG RVYSNLSNL RQHMKLIHY 
      PTYVQCPVC KKPFKTDLY LRRHVMSAH EGPKTGPRN 
      TPLRLRRRR SSDHWPPEE RVRDKGRNR LIKLGEGIE 
      IYEDQLRSV KWNDYRKLT RGLATILFS PAELATCSV 
      TGQRWSRAG SGERPVKPA LDRSKVQAI ISYVATRFP 
      MVEISRIKQ VLAYKCKEN STAFKMKAV RYYEDQHQK
    CDS sequence
    • >TC012869 RA
      atggctcta gccgatgac cagcaattt tgtcttaga 
      tggaacaac tttcaagcg aatattaca tcacaattc 
      gaggctttg agggacgat gaagacttc acagacgtc 
      acgatcgcc tgcgagggg cagcgcatg caagcacac 
      aaagtcgtg ctttcggcg tgcagcccc tttttcaaa 
      gaactcttc aaaacgaat ccctgttca catccgatt 
      attttcatg cgtgatgtg gaggccaga cacatagtg 
      gccctgatg gagtttatg tatgccggc gaggtgaac 
      gtggcccag gcccacctc tcggcgttc cttaagaca 
      gcagagtcg ctaaaaatc cggggatta accgacact 
      tcggccgaa agcgaacaa aaagacgaa gacactctt 
      tatctcaac cctcagcct tccaaaccc ggcaaacac 
      aaagtttcc tcctcaagc actgtaact tctagtcag 
      gacgtttca aacacgagt gtttcaagc cccccgtca 
      aagagacaa tgcaagtct gagccggaa gttccacgg 
      gtgaagatc aaagaagac gctattaac ccgttcgcc 
      ctgcaggaa ccccgcaac caggacctg gtgcagccc 
      aagatggag cttccggac tacctgagc gatgacgac 
      ggtcgcgag tcgcatttc tacgctggc gacgttacc 
      gagcttcca ggtggcatg gagatcatc ccccaagtg 
      gcgggcttc gtcaaggag cagccccag gagtccgct 
      ccagacacc cgtaagctg cactcgctg gaccccagg 
      ccgtgtgag gagtgcggc cgcgtttac agcaattta 
      tcgaacttg cgccagcac atgaaactc attcattac 
      ccgacctat gtgcaatgt cccgtttgt aaaaaaccg 
      ttcaagacc gatttgtac ctccggagg cacgtgatg 
      agcgcgcac gagggcccc aagacgggg ccccggaac 
      acccccttg agactgcgg cggcgccgc tcgtccgac 
      cactggcct cccgaggag cgagtgcgc gacaaaggg 
      agaaataga ctgatcaaa ctaggtgaa gggattgaa 
      atctacgag gatcaatta cgtagcgtc aaatggaac 
      gactacagg aagttgact agaggtctt gcgacgatt 
      ttgtttagt ccggccgag ttggccacg tgttcggtc 
      actggtcag aggtggagt agggccggg agtggcgag 
      aggccagtg aagccggcc ttagaccgc tccaaagtc 
      caggcgatt atttcttac gtggccacg aggttcccc 
      atggttgag attagccga atcaagcaa gttttggcc 
      tacaagtgc aaggaaaac tcgacggct tttaaaatg 
      aaagcggtg cgctattac gaggatcag catcagaag 
      tga
    mRNA sequence
    • >TC012869 RA
      ttctttttc ttggttggc caaaagaag tagacatat 
      attgattaa aattaaaag gtagtttat ttttatgtc 
      tgagatggt cttcctacc tagcgataa aatttcgca 
      aaattgagt gtgaaacgt gccgaaacg atggctcta 
      gccgatgac cagcaattt tgtcttaga tggaacaac 
      tttcaagcg aatattaca tcacaattc gaggctttg 
      agggacgat gaagacttc acagacgtc acgatcgcc 
      tgcgagggg cagcgcatg caagcacac aaagtcgtg 
      ctttcggcg tgcagcccc tttttcaaa gaactcttc 
      aaaacgaat ccctgttca catccgatt attttcatg 
      cgtgatgtg gaggccaga cacatagtg gccctgatg 
      gagtttatg tatgccggc gaggtgaac gtggcccag 
      gcccacctc tcggcgttc cttaagaca gcagagtcg 
      ctaaaaatc cggggatta accgacact tcggccgaa 
      agcgaacaa aaagacgaa gacactctt tatctcaac 
      cctcagcct tccaaaccc ggcaaacac aaagtttcc 
      tcctcaagc actgtaact tctagtcag gacgtttca 
      aacacgagt gtttcaagc cccccgtca aagagacaa 
      tgcaagtct gagccggaa gttccacgg gtgaagatc 
      aaagaagac gctattaac ccgttcgcc ctgcaggaa 
      ccccgcaac caggacctg gtgcagccc aagatggag 
      cttccggac tacctgagc gatgacgac ggtcgcgag 
      tcgcatttc tacgctggc gacgttacc gagcttcca 
      ggtggcatg gagatcatc ccccaagtg gcgggcttc 
      gtcaaggag cagccccag gagtccgct ccagacacc 
      cgtaagctg cactcgctg gaccccagg ccgtgtgag 
      gagtgcggc cgcgtttac agcaattta tcgaacttg 
      cgccagcac atgaaactc attcattac ccgacctat 
      gtgcaatgt cccgtttgt aaaaaaccg ttcaagacc 
      gatttgtac ctccggagg cacgtgatg agcgcgcac 
      gagggcccc aagacgggg ccccggaac acccccttg 
      agactgcgg cggcgccgc tcgtccgac cactggcct 
      cccgaggag cgagtgcgc gacaaaggg agaaataga 
      ctgatcaaa ctaggtgaa gggattgaa atctacgag 
      gatcaatta cgtagcgtc aaatggaac gactacagg 
      aagttgact agaggtctt gcgacgatt ttgtttagt 
      ccggccgag ttggccacg tgttcggtc actggtcag 
      aggtggagt agggccggg agtggcgag aggccagtg 
      aagccggcc ttagaccgc tccaaagtc caggcgatt 
      atttcttac gtggccacg aggttcccc atggttgag 
      attagccga atcaagcaa gttttggcc tacaagtgc 
      aaggaaaac tcgacggct tttaaaatg aaagcggtg 
      cgctattac gaggatcag catcagaag tgaatatct 
      ctagattag ggaaacaat atcatgtgt caaaaaata 
      tttccagtg tttatctct ccaagtgtg tatatttag 
      ttatttgtt atcaggttt tgtttttta tttatttat 
      tggataata aagtattga atttaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY