Status
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Gene information
General information
TC012519 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10946605..10950644
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012519
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01987

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    remarks: later than schedule, hours 48 adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Pupal morphology
    remarks: later than schedule, hours 48
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012519 RA
      MKPPLILIS LLIHFSRGA SDFDEKIEL LKDIEIPDI 
      DDFDIVHEK CDKEGGNGT YDKLKTSLD TIESCISDF 
      VDVKVLKTE IENSKKTGA LDEVFGKYC AKRFKLATC 
      LGDFVNNLR SCLTSDEKT ALNLTTNIL TQLGEFVCY 
      KDGDRLAMF VAEGGTECF KARAKGIQS CVNATLKII 
      PSSFSTNPL PNLLIDRKK CDDLGKLQD CMVEELEKC 
      SDTTPANII DALFRFIKR SACPKKNKR SPSFHRILR 
      RNIPPATYS ADRIAALLQ NKCSQNAPH DMKRIRESF 
      THARICIQI VNDHEENLL TIFSLKELK ENFKECSDD 
      FVETAELCF GDDEKYFPK FVQQIYFNL LEVAYKHKD 
      LILNKTAME KYNDCVKIL REPGNAAAL YHCIFQGPV 
      SKIWTKEVV CENLMNGTA CAIDEVKKK CSSDIFDDF 
      VDDFVHFWK SSCKPNQN
    CDS sequence
    • >TC012519 RA
      atgaagcca ccactaatt ttaatatcg ctcctgatc 
      catttttcc cgcggagcc tcggatttc gacgaaaag 
      atcgagctt ttgaaagac atcgaaatc ccagacatt 
      gacgatttt gacatagtc cacgaaaaa tgtgataaa 
      gaaggcgga aacggaacc tacgacaaa ctaaagaca 
      tccctcgac acaattgag agctgtatc agcgatttc 
      gtggacgta aaagtcctc aaaaccgaa atcgaaaat 
      tcgaaaaag accggcgct ttggacgaa gtgttcggc 
      aaatattgc gccaagcgc ttcaaactt gcaacgtgt 
      cttggagac ttcgtcaat aatcttcgc tcctgtttg 
      actagtgat gaaaaaacc gcccttaac ttgaccacg 
      aatattttg acccaattg ggcgagttt gtgtgttat 
      aaggatggc gaccggctt gctatgttt gtggccgag 
      ggtgggacc gagtgtttc aaagctcgg gctaaggga 
      atccagagc tgtgtcaac gccacgctt aaaatcatt 
      ccttccagc tttagtaca aacccgtta cccaaccta 
      ctgattgac aggaaaaaa tgcgacgat ttaggcaag 
      ctccaggac tgcatggtc gaagagttg gaaaagtgt 
      tcagacacc actcccgcg aacattatt gacgcgctt 
      tttaggttt atcaagcgc tcagcgtgt cccaagaaa 
      aacaagcgg agtccaagc tttcaccgc attttgagg 
      agaaatata ccgcctgcc acatacagc gctgatcgc 
      attgcagct ttattgcag aacaagtgc agtcagaat 
      gctccccat gacatgaaa cgcatcaga gaatctttc 
      acacatgcg agaatttgt atccaaatt gtaaacgac 
      cacgaagaa aatcttctg acaatcttc tccttgaag 
      gagcttaag gagaatttt aaagaatgc agcgacgat 
      ttcgtcgaa acggctgaa ctgtgtttt ggcgacgac 
      gaaaaatat tttcccaaa ttcgtccag cagatttat 
      tttaatcta ttagaggtt gcctacaaa cacaaggac 
      ctgatactg aacaaaaca gcgatggaa aagtataac 
      gactgtgtt aaaattttg agagagcca ggcaatgcg 
      gccgcatta tatcactgt atttttcag gggcctgtg 
      tccaaaatc tggacaaaa gaagtcgtg tgcgagaat 
      ttaatgaat ggaactgca tgtgctatt gatgaggtc 
      aagaaaaag tgctccagc gatattttc gatgatttt 
      gttgacgat ttcgtccat ttttggaaa tcctcttgc 
      aaaccaaat cagaactaa
    mRNA sequence
    • >TC012519 RA
      cagacacag ttggaaagg taacagggt attccacct 
      aatttcccc aaattaaac attagataa actactgat 
      tttttaaaa tagcatgat cgcaatttg ctaaaaaaa 
      tattaattg taacgtttg tagcaagat ttgttaagt 
      ttttgtgta atatatttt taaaagttt caattaatt 
      taattttta attgtcgac cagtaaagg cttaactca 
      aattatact caaaaatgt aggctgttt tatttaaaa 
      aaaaattct attatctca tttttttaa ataacttat 
      acacctgaa gacaacttc tgatgtagt tactactta 
      ttagttatt agtatgtct tctgtataa atatttttt 
      taatattcg tacaaaaac agacttaaa tgcacatta 
      aaagtgatg taaatccga aatactcga aaaaatcgg 
      agaaaatca aattttttg ctatttttg gcccttgat 
      accaaacag atgggtaaa tccatcact gagactggc 
      cgtgaattt tcgtccaaa ccagttttg tatttttaa 
      gttcgattt ttgtattta ttgcccaaa aacctaaca 
      caaccccaa actttttcg agggttgat agtcagccc 
      ttcctctct tgtgccaaa tattcgcgt ggggtgttt 
      tcctatcca cgagaatcg cctttccag caaccccgt 
      caagccaag acctgttct ctcactgca tcagcacaa 
      cgcacttta aatccacct tgagtagaa tagggggaa 
      acgtgtaag gtggacacg cccttcgtc aagacttac 
      gctactctg ggctacctt tagctaccg caactgtca 
      agagatcaa gtgacgttt gctcacaac ttctatttg 
      attttctta attttgttt caagtttac ctacccaaa 
      gttaggtat tgtcaaaaa caagtttga ttatgtcag 
      gtttagcgg ggaaaaatg attccaaag gacggtttt 
      aagagtgga ggttcacca gagaagggt caaattttc 
      aaacggttt agggcataa cggaacggt aacaatgtc 
      ggcattaga aatagactt tcttgagcc cttggtcat 
      tatctgatg ttgacagct tcacgttcc gccgtcccc 
      tactaaagt caggaactg cacctgatt cttctgtac 
      cagtacact ctcaatata aatacgtgg cattgtcgt 
      attataatc aatgtaact gtaggtgtt gaaaaaaaa 
      taaaaacgg atagggttg attaacggt gcagttatc 
      gactttcca atatccaca taaaaattg atgtttgaa 
      cactttcgt aagtaatcg agcacaata ttctcttaa 
      tgtcgtatc gttgtatta gatatagag catttaatt 
      atacgtatt gtagcctag tggtgtatc aatgttcag 
      tgcagtttc taactttcc caaaacttt cggccaagt 
      ttccaaaat tcaaaattt ttcgttcgg gttcgcgat 
      ttacaaaag ggaaaatct cctttggaa tcatctcct 
      tccgacgat gtttcagtt ccagtcccc ggaataaat 
      tgcgattgt gagcaacac atttccaag ttaacttaa 
      atgtatcca ggccatttt gatgcaaat tctggccta 
      tatacgtat ttgttgttt aataaacgt aggttgact 
      gttattgct tgacacaaa aatgttaaa aaaaatctg 
      atggcctca ttaatattt gatagaagt accagtggt 
      gtatataaa accctccgc ctccagctg gggtttcaa 
      ttaaatact caattaaag ttaacatta tattcaaac 
      atgcaatgt tacttattg aagctattt ttatgataa 
      atgttttcg tctcttaga atattgtgg taagcgtca 
      aaatatata tgctgcctg acagtgaga gagttagtg 
      atgtatata gttttataa taatagcta ttataacgt 
      attattaaa aatgttacc acttaatgt cctaaaaca 
      ttttatttc atagaattg ttgataggc aatgttatt 
      ataaaatta ttgtagatt aatgaagta aaagaaatg 
      tgatttaat ctcgcgagt ctcattccc aaccctcca 
      cccactcca gtcaacgaa tttgacctc taattggca 
      aagtcaagg agtcaaact caatcaatt cagtgtgaa 
      atccgcaac gaaaatgaa gccaccact aattttaat 
      atcgctcct gatccattt ttcccgcgg agcctcgga 
      tttcgacga aaagatcga gcttttgaa agacatcga 
      aatcccaga cattgacga ttttgacat agtccacga 
      aaaatgtga taaagaagg cggaaacgg aacctacga 
      caaactaaa gacatccct cgacacaat tgagagctg 
      tatcagcga tttcgtgga cgtaaaagt cctcaaaac 
      cgaaatcga aaattcgaa aaagaccgg cgctttgga 
      cgaagtgtt cggcaaata ttgcgccaa gcgcttcaa 
      acttgcaac gtgtcttgg agacttcgt caataatct 
      tcgctcctg tttgactag tgatgaaaa aaccgccct 
      taacttgac cacgaatat tttgaccca attgggcga 
      gtttgtgtg ttataagga tggcgaccg gcttgctat 
      gtttgtggc cgagggtgg gaccgagtg tttcaaagc 
      tcgggctaa gggaatcca gagctgtgt caacgccac 
      gcttaaaat cattccttc cagctttag tacaaaccc 
      gttacccaa cctactgat tgacaggaa aaaatgcga 
      cgatttagg caagctcca ggactgcat ggtcgaaga 
      gttggaaaa gtgttcaga caccactcc cgcgaacat 
      tattgacgc gctttttag gtttatcaa gcgctcagc 
      gtgtcccaa gaaaaacaa gcggagtcc aagctttca 
      ccgcatttt gaggagaaa tataccgcc tgccacata 
      cagcgctga tcgcattgc agctttatt gcagaacaa 
      gtgcagtca gaatgctcc ccatgacat gaaacgcat 
      cagagaatc tttcacaca tgcgagaat ttgtatcca 
      aattgtaaa cgaccacga agaaaatct tctgacaat 
      cttctcctt gaaggagct taaggagaa ttttaaaga 
      atgcagcga cgatttcgt cgaaacggc tgaactgtg 
      ttttggcga cgacgaaaa atattttcc caaattcgt 
      ccagcagat ttattttaa tctattaga ggttgccta 
      caaacacaa ggacctgat actgaacaa aacagcgat 
      ggaaaagta taacgactg tgttaaaat tttgagaga 
      gccaggcaa tgcggccgc attatatca ctgtatttt 
      tcaggggcc tgtgtccaa aatctggac aaaagaagt 
      cgtgtgcga gaatttaat gaatggaac tgcatgtgc 
      tattgatga ggtcaagaa aaagtgctc cagcgatat 
      tttcgatga ttttgttga cgatttcgt ccatttttg 
      gaaatcctc ttgcaaacc aaatcagaa ctaaaatgt 
      ttattatcc tacgttcaa tttattgga agtatttta 
      aaatttaac actatgtac cgaatgctg aataaatta 
      acctttaac cg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY