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Gene information
General information
TC031245
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012517
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10881882..10889216
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031245
    iBeetle screen iB_01986

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09477

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031245 RA
      MKHSTPNAC FLQAISSPE ESDESLTSL QQTQRKTAA 
      SKTGARRAS DAGHKKSTR SASSDSNTS TESTASSNA 
      IRTTGAPFT TEEVKQKYL SRGSVDATP RTPTKEPTK 
      PFQSRFLHN KTTTPPSAT KDDDETETS SEEETESEE 
      ESDEDTKKP DTKEMAKTD IGPLLARSA HARDGSAEN 
      ARKSSRDET STYTRPRYG VPKEESPPR SRYARRTSG 
      AQDEEPPRY GYTSRFLNK SKSSAAIPP EEDDHKYSS 
      TLGDDSDSK YGSGRSRYM ALKERRQRL ARSRSSQQF 
      GDEEDVDEP ISPTSCNPS AYLASRGYG SAASSGHDL 
      ARSRSSHAL KSRDNSPDR SNATEKDGA ALSSWARYL 
      KNKYGNRNS GKDKESSSS VTPSSSSTT ARRLSLGLP 
      LRSSTELGS SDDDQKNMQ GSPTTPPTA ATAAAGIAV 
      AVAGTSPRS QYLQKCQQL FEIGSRGSE AGCFTWPRG 
      VAVGPDNSI VVADSSNHR VQVFDSNGR FLKEFGQYG 
      NGEGEFDCL AGVAVNRIG QFIIADRYN HRIQVFDPS 
      GRFLRAFGS QGTADGRFN YPWGITTDA LGFIYVCDK 
      ENHRVQVFQ SDGTFVGKF GSMGSKEGQ LEHPHYIAV 
      SNTNRVVVS DSNNHRIQI FDVNGKVLS SFGSEGSDD 
      GQFKFPRGV AVDDQGYIC VADSGNNRI QIFHPDGTF 
      LRSFGCWGI GKGEFKGLE GVAMTPNGH ILVCDRENH 
      RIQVF
    • >TC031245 RB
      MEQFEQLLT CAICLDRYR NPKLLPCQH SFCMEPCLE 
      GLVDYVRRQ VKCPECRAE HRIPYQGVQ GFPTNVTLQ 
      RFLELHIEI TGELPDPTS GQVMERCNV CSEKAYCSF 
      CSHCDKKIC EECKGAHME ILRREISRI NNQIRRGIH 
      RLQDTLSLV EKNTQSIQS NCSSVSEEV EEIYRRLSK 
      ALKDRTEYL RGEIDRYLG TELRSLTNL KENLEQEIS 
      NILSNCDLA DKHMNENVD IWDDCELMD AKEIFLKTV 
      EFIRNFEYE NTDYSRKIR LVLAHDPNQ LVLHVAGYG 
      DLNIANQHF SQGSGMLQP PGPGLMRSK SDHRLASQF 
      RQQEERGYE RGYGGDQDE ALLGGRKFG ERPKTERYG 
      DRYRGTDYG EDYDNDSRP ARSRYSRFR RHGQDNDSD 
      GEQPGRGVR FEPQKVERE KVLDTEDVA RGPLSGITR 
      LADSPRVMK KLQENESGK KKEKEAPPP PAPQPKVVP 
      KRPPPPTNR QVSEEDEIA KIKKQNKGA TTSTETETR 
      PERTEERHS AQRKTPAPE VSLDEP
    • >TC031245 RC
      MEQFEQLLT CAICLDRYR NPKLLPCQH SFCMEPCLE 
      GLVDYVRRQ VKCPECRAE HRIPYQGVQ GFPTNVTLQ 
      RFLELHIEI TGELPDPTS GQVMERCNV CSEKAYCSF 
      CSHCDKKIC EECKGAHME ILRREISRI NNQIRRGIH 
      RLQDTLSLV EKNTQSIQS NCSSVSEEV EEIYRRLSK 
      ALKDRTEYL RGEIDRYLG TELRSLTNL KENLEQEIS 
      NILSNCDLA DKHMNENVD IWDDCELMD AKEIFLKTV 
      EFIRNFEYE NTDYSRKIR LVLAHDPNQ LVLHVAGYG 
      DLNIANQHF SQGSGMLQP PGPGLMRSK SDHRLASQF 
      RQQEERGYE RGYGGDQDE ALLGGRKFG ERPKTERYG 
      DRYRGTDYG EDYDNDSRP ARSRYSRFR RHGQDNDSD 
      GEQPGRGVR FEPQKVERE KVLDTEDVA RGPLSGITR 
      LADSPRVMK KLQENESGK KKEKEAPPP PAPQPKVVP 
      KRPPPPTNR QVSEEDEIA KIKKQNKGA TTSTETETR 
      PERTEERHS AQRKTPAPE AISSPEESD ESLTSLQQT 
      QRKTAASKT GARRASDAG HKKSTRSAS SDSNTSTES 
      TASSNAIRT TGAPFTTEE VKQKYLSRG SVDATPRTP 
      TKEPTKPFQ SRFLHNKTT TPPSATKDD DETETSSEE 
      ETESEEESD EDTKKPDTK EMAKTDIGP LLARSAHAR 
      DGSAENARK SSRDETSTY TRPRYGVPK EESPPRSRY 
      ARRTSGAQD EEPPRYGYT SRFLNKSKS SAAIPPEED 
      DHKYSSTLG DDSDSKYGS GRSRYMALK ERRQRLARS 
      RSSQQFGDE EDVDEPISP TSCNPSAYL ASRGYGSAA 
      SSGHDLARS RSSHALKSR DNSPDRSNA TEKDGAALS 
      SWARYLKNK YGNRNSGKD KESSSSVTP SSSSTTARR 
      LSLGLPLRS STELGSSDD DQKNMQGSP TTPPTAATA 
      AAGIAVAVA GTSPRSQYL QKCQQLFEI GSRGSEAGC 
      FTWPRGVAV GPDNSIVVA DSSNHRVQV FDSNGRFLK 
      EFGQYGNGE GEFDCLAGV AVNRIGQFI IADRYNHRI 
      QVFDPSGRF LRAFGSQGT ADGRFNYPW GITTDALGF 
      IYVCDKENH RVQVFQSDG TFVGKFGSM GSKEGQLEH 
      PHYIAVSNT NRVVVSDSN NHRIQIFDV NGKVLSSFG 
      SEGSDDGQF KFPRGVAVD DQGYICVAD SGNNRIQIF 
      HPDGTFLRS FGCWGIGKG EFKGLEGVA MTPNGHILV 
      CDRENHRIQ VF
    CDS sequence
    • >TC031245 RA
      atgaagcat tcaacacct aacgcatgt tttctccag 
      gccatctcc agccctgaa gaatccgac gaatctctc 
      acttccttg caacaaaca cagcgcaaa accgccgcc 
      agtaaaaca ggcgcgaga cgcgcctcc gacgctgga 
      cataaaaaa tccacgcga tcggcgagc tccgactcg 
      aacacttcc acagaatcc acagcttcc tcaaacgcc 
      attcgcaca accggggcc ccgttcacc accgaagaa 
      gtcaaacag aagtatctc tcacgcggg agtgtcgac 
      gccactccc cgaacccca accaaagaa ccaaccaaa 
      ccgttccaa agccgcttc ctccacaac aaaaccacg 
      acaccgcca tccgccacc aaagacgac gacgagacc 
      gaaaccagt tcggaggag gagacggaa agtgaggag 
      gaatccgac gaggacacc aaaaaacca gacaccaaa 
      gaaatggcg aagactgac attggcccc cttttggcc 
      cgcagcgcg cacgcgagg gacgggagc gccgaaaac 
      gcccggaaa agcagccgg gacgaaacc tccacgtac 
      accagaccc aggtacggg gtcccgaag gaggaatcg 
      cccccgcgg tcgcgctac gcccggcga acctcaggg 
      gcgcaagac gaggagccc cccaggtac ggctacacg 
      agccgcttc ctcaacaag agcaaaagc tcggctgcc 
      atcccgcct gaagaagac gaccacaag tacagttcg 
      acgcttggc gacgactcc gacagtaaa tatgggagc 
      gggcggtcg cgctacatg gccctgaag gagcggaga 
      cagaggctg gcgcgcagt cggagcagt cagcagttt 
      ggcgacgaa gaggacgtc gatgagccc atctcgccg 
      acgtcctgc aacccgtcc gcgtacttg gcgtcgcgg 
      gggtacggc tccgcagca tcgtcaggg cacgatctt 
      gcgagaagt cgctcctcg catgcgttg aagtcgcgc 
      gataattcg cctgatagg tcgaacgcg acggagaaa 
      gatggggcc gcgttgagt tcttgggcg aggtattta 
      aagaacaag tacgggaat aggaacagt ggcaaagat 
      aaggaatcg agtagttcg gtgacaccg tcgagtagt 
      tcgacgacg gcgaggaga ctgtctctt ggtctcccg 
      ctgaggtcg tccacggag ttagggagc tcggacgac 
      gaccaaaaa aacatgcaa ggctccccc actacccct 
      cctacagca gctacggcg gcagccggt atcgcagtg 
      gcagtggca ggtacctcc cctaggagt cagtacttg 
      cagaaatgc caacagctg ttcgaaatt ggtagtcgg 
      gggagcgaa gccggatgt ttcacctgg cctcgcggc 
      gttgccgtg ggccccgat aactccatc gtagtcgcg 
      gattcctcc aaccaccgc gtccaagtc ttcgactca 
      aacggacga ttcctgaaa gaatttgga caatacggc 
      aacggcgaa ggcgaattc gactgtctt gcaggcgtt 
      gccgtcaac agaattggc caatttatc atcgccgac 
      cggtacaac caccgaatc caagtcttc gacccttcg 
      ggacgcttc ttgcgagcc ttcggcagc cagggcacc 
      gccgacggc cgcttcaac tacccgtgg ggcatcacc 
      accgacgcc ctaggcttc atctacgtg tgtgacaag 
      gaaaaccac agagtgcag gttttccag tccgatggt 
      accttcgtg ggcaagttc ggctccatg ggcagcaag 
      gagggccaa ctggagcat ccccactac atcgccgtt 
      tctaacacg aacagggtc gttgtttcg gactcgaac 
      aaccaccga atccagata tttgacgtg aatggcaaa 
      gtcctgtcc tccttcggg tccgagggc tcggacgat 
      ggtcagttc aagttcccc agaggcgtt gctgtggat 
      gaccaaggc tacatttgc gttgctgat tccggaaat 
      aacagaatc cagattttc caccccgat ggaacattt 
      ttgcgttca ttcggttgt tggggcatc ggcaaaggc 
      gagttcaag ggccttgaa ggtgtcgca atgaccccc 
      aatggacac atactggtg tgtgatcga gaaaaccac 
      aggatacaa gtgttttaa
    • >TC031245 RB
      atggaacaa ttcgagcaa cttttaaca tgtgccatt 
      tgtctggac cgctaccgc aaccccaag ctgttgcca 
      tgccagcac agcttctgc atggaacca tgtctggag 
      ggcttggtg gattacgtc cggagacag gtgaagtgt 
      ccggaatgc cgagcggaa caccgcatc ccctatcag 
      ggggtgcag ggtttcccg accaacgtc acccttcaa 
      agattcctg gagcttcat attgaaata actggcgaa 
      ctcccggat ccaacaagt ggccaagtt atggaacga 
      tgtaacgtt tgctcagaa aaggcgtat tgtagtttt 
      tgttcccac tgtgataag aaaatctgt gaagaatgc 
      aagggagcg cacatggaa atcctccgg cgggagatc 
      tcccggatt aacaaccag atccggcga ggcattcat 
      aggttacaa gacactttg tctctagtc gagaaaaac 
      acccaaagt attcaatcg aactgctcg tcggtgagc 
      gaagaagtc gaggagatt taccggagg ctctccaaa 
      gccctcaaa gaccggacg gaatattta cgaggcgag 
      atcgatcgc tatttagga acggaatta aggagtttg 
      accaacttg aaggagaac ttggagcaa gagatatcc 
      aacattttg agcaactgc gacctggcc gacaagcac 
      atgaacgag aacgtggac atttgggac gactgcgag 
      ctcatggac gccaaagaa atcttcctc aagacagtc 
      gaattcatc cggaacttc gaatacgaa aacacggat 
      tacagcagg aaaattcgg ctggtttta gcgcacgac 
      ccaaatcaa ctcgtcctg cacgtcgcc ggctacggc 
      gatctaaac atagccaat cagcacttt agtcaaggc 
      tcgggcatg ttgcaacct ccaggaccg ggcttaatg 
      cggtccaaa agcgaccac cggctagcc tcacagttc 
      aggcagcag gaggaaaga gggtatgag agaggttac 
      ggcggcgac caagacgag gcgctcctc ggaggccgc 
      aaattcggc gaaaggccc aaaacggag agatacggt 
      gacagatac cgtggcact gactacggc gaagactac 
      gataacgac tccagaccg gctaggtct aggtacagc 
      cgcttcagg aggcacggc caagataac gactccgac 
      ggggagcaa ccgggtcgt ggagtcaga ttcgaaccg 
      cagaaggtt gagcgggag aaggtcctg gacacggaa 
      gacgtggcc agaggcccc ttgagcggc atcacccgc 
      ctggctgac tcgccccgc gtcatgaaa aagctccaa 
      gagaacgaa agcggcaaa aagaaggaa aaggaggct 
      cctccacca cccgccccc cagccaaaa gtcgtacca 
      aagcggccc ccgcccccc acaaacaga caagtaagc 
      gaagaagac gagatcgct aaaataaag aaacagaat 
      aaaggtgcc accacctcc accgaaaca gagacccgg 
      ccagagcgc accgaagaa agacactca gctcagaga 
      aaaaccccc gcccccgag gtgagtcta gacgagccg 
      taa
    • >TC031245 RC
      atggaacaa ttcgagcaa cttttaaca tgtgccatt 
      tgtctggac cgctaccgc aaccccaag ctgttgcca 
      tgccagcac agcttctgc atggaacca tgtctggag 
      ggcttggtg gattacgtc cggagacag gtgaagtgt 
      ccggaatgc cgagcggaa caccgcatc ccctatcag 
      ggggtgcag ggtttcccg accaacgtc acccttcaa 
      agattcctg gagcttcat attgaaata actggcgaa 
      ctcccggat ccaacaagt ggccaagtt atggaacga 
      tgtaacgtt tgctcagaa aaggcgtat tgtagtttt 
      tgttcccac tgtgataag aaaatctgt gaagaatgc 
      aagggagcg cacatggaa atcctccgg cgggagatc 
      tcccggatt aacaaccag atccggcga ggcattcat 
      aggttacaa gacactttg tctctagtc gagaaaaac 
      acccaaagt attcaatcg aactgctcg tcggtgagc 
      gaagaagtc gaggagatt taccggagg ctctccaaa 
      gccctcaaa gaccggacg gaatattta cgaggcgag 
      atcgatcgc tatttagga acggaatta aggagtttg 
      accaacttg aaggagaac ttggagcaa gagatatcc 
      aacattttg agcaactgc gacctggcc gacaagcac 
      atgaacgag aacgtggac atttgggac gactgcgag 
      ctcatggac gccaaagaa atcttcctc aagacagtc 
      gaattcatc cggaacttc gaatacgaa aacacggat 
      tacagcagg aaaattcgg ctggtttta gcgcacgac 
      ccaaatcaa ctcgtcctg cacgtcgcc ggctacggc 
      gatctaaac atagccaat cagcacttt agtcaaggc 
      tcgggcatg ttgcaacct ccaggaccg ggcttaatg 
      cggtccaaa agcgaccac cggctagcc tcacagttc 
      aggcagcag gaggaaaga gggtatgag agaggttac 
      ggcggcgac caagacgag gcgctcctc ggaggccgc 
      aaattcggc gaaaggccc aaaacggag agatacggt 
      gacagatac cgtggcact gactacggc gaagactac 
      gataacgac tccagaccg gctaggtct aggtacagc 
      cgcttcagg aggcacggc caagataac gactccgac 
      ggggagcaa ccgggtcgt ggagtcaga ttcgaaccg 
      cagaaggtt gagcgggag aaggtcctg gacacggaa 
      gacgtggcc agaggcccc ttgagcggc atcacccgc 
      ctggctgac tcgccccgc gtcatgaaa aagctccaa 
      gagaacgaa agcggcaaa aagaaggaa aaggaggct 
      cctccacca cccgccccc cagccaaaa gtcgtacca 
      aagcggccc ccgcccccc acaaacaga caagtaagc 
      gaagaagac gagatcgct aaaataaag aaacagaat 
      aaaggtgcc accacctcc accgaaaca gagacccgg 
      ccagagcgc accgaagaa agacactca gctcagaga 
      aaaaccccc gcccccgag gccatctcc agccctgaa 
      gaatccgac gaatctctc acttccttg caacaaaca 
      cagcgcaaa accgccgcc agtaaaaca ggcgcgaga 
      cgcgcctcc gacgctgga cataaaaaa tccacgcga 
      tcggcgagc tccgactcg aacacttcc acagaatcc 
      acagcttcc tcaaacgcc attcgcaca accggggcc 
      ccgttcacc accgaagaa gtcaaacag aagtatctc 
      tcacgcggg agtgtcgac gccactccc cgaacccca 
      accaaagaa ccaaccaaa ccgttccaa agccgcttc 
      ctccacaac aaaaccacg acaccgcca tccgccacc 
      aaagacgac gacgagacc gaaaccagt tcggaggag 
      gagacggaa agtgaggag gaatccgac gaggacacc 
      aaaaaacca gacaccaaa gaaatggcg aagactgac 
      attggcccc cttttggcc cgcagcgcg cacgcgagg 
      gacgggagc gccgaaaac gcccggaaa agcagccgg 
      gacgaaacc tccacgtac accagaccc aggtacggg 
      gtcccgaag gaggaatcg cccccgcgg tcgcgctac 
      gcccggcga acctcaggg gcgcaagac gaggagccc 
      cccaggtac ggctacacg agccgcttc ctcaacaag 
      agcaaaagc tcggctgcc atcccgcct gaagaagac 
      gaccacaag tacagttcg acgcttggc gacgactcc 
      gacagtaaa tatgggagc gggcggtcg cgctacatg 
      gccctgaag gagcggaga cagaggctg gcgcgcagt 
      cggagcagt cagcagttt ggcgacgaa gaggacgtc 
      gatgagccc atctcgccg acgtcctgc aacccgtcc 
      gcgtacttg gcgtcgcgg gggtacggc tccgcagca 
      tcgtcaggg cacgatctt gcgagaagt cgctcctcg 
      catgcgttg aagtcgcgc gataattcg cctgatagg 
      tcgaacgcg acggagaaa gatggggcc gcgttgagt 
      tcttgggcg aggtattta aagaacaag tacgggaat 
      aggaacagt ggcaaagat aaggaatcg agtagttcg 
      gtgacaccg tcgagtagt tcgacgacg gcgaggaga 
      ctgtctctt ggtctcccg ctgaggtcg tccacggag 
      ttagggagc tcggacgac gaccaaaaa aacatgcaa 
      ggctccccc actacccct cctacagca gctacggcg 
      gcagccggt atcgcagtg gcagtggca ggtacctcc 
      cctaggagt cagtacttg cagaaatgc caacagctg 
      ttcgaaatt ggtagtcgg gggagcgaa gccggatgt 
      ttcacctgg cctcgcggc gttgccgtg ggccccgat 
      aactccatc gtagtcgcg gattcctcc aaccaccgc 
      gtccaagtc ttcgactca aacggacga ttcctgaaa 
      gaatttgga caatacggc aacggcgaa ggcgaattc 
      gactgtctt gcaggcgtt gccgtcaac agaattggc 
      caatttatc atcgccgac cggtacaac caccgaatc 
      caagtcttc gacccttcg ggacgcttc ttgcgagcc 
      ttcggcagc cagggcacc gccgacggc cgcttcaac 
      tacccgtgg ggcatcacc accgacgcc ctaggcttc 
      atctacgtg tgtgacaag gaaaaccac agagtgcag 
      gttttccag tccgatggt accttcgtg ggcaagttc 
      ggctccatg ggcagcaag gagggccaa ctggagcat 
      ccccactac atcgccgtt tctaacacg aacagggtc 
      gttgtttcg gactcgaac aaccaccga atccagata 
      tttgacgtg aatggcaaa gtcctgtcc tccttcggg 
      tccgagggc tcggacgat ggtcagttc aagttcccc 
      agaggcgtt gctgtggat gaccaaggc tacatttgc 
      gttgctgat tccggaaat aacagaatc cagattttc 
      caccccgat ggaacattt ttgcgttca ttcggttgt 
      tggggcatc ggcaaaggc gagttcaag ggccttgaa 
      ggtgtcgca atgaccccc aatggacac atactggtg 
      tgtgatcga gaaaaccac aggatacaa gtgttttaa
    mRNA sequence
    • >TC031245 RA
      gattacaca ttggcatgc cacgactat ccactcttc 
      cctgtgttg tgtgtgtga agcatgaag cattcaaca 
      cctaacgca tgttttctc caggccatc tccagccct 
      gaagaatcc gacgaatct ctcacttcc ttgcaacaa 
      acacagcgc aaaaccgcc gccagtaaa acaggcgcg 
      agacgcgcc tccgacgct ggacataaa aaatccacg 
      cgatcggcg agctccgac tcgaacact tccacagaa 
      tccacagct tcctcaaac gccattcgc acaaccggg 
      gccccgttc accaccgaa gaagtcaaa cagaagtat 
      ctctcacgc gggagtgtc gacgccact ccccgaacc 
      ccaaccaaa gaaccaacc aaaccgttc caaagccgc 
      ttcctccac aacaaaacc acgacaccg ccatccgcc 
      accaaagac gacgacgag accgaaacc agttcggag 
      gaggagacg gaaagtgag gaggaatcc gacgaggac 
      accaaaaaa ccagacacc aaagaaatg gcgaagact 
      gacattggc ccccttttg gcccgcagc gcgcacgcg 
      agggacggg agcgccgaa aacgcccgg aaaagcagc 
      cgggacgaa acctccacg tacaccaga cccaggtac 
      ggggtcccg aaggaggaa tcgcccccg cggtcgcgc 
      tacgcccgg cgaacctca ggggcgcaa gacgaggag 
      ccccccagg tacggctac acgagccgc ttcctcaac 
      aagagcaaa agctcggct gccatcccg cctgaagaa 
      gacgaccac aagtacagt tcgacgctt ggcgacgac 
      tccgacagt aaatatggg agcgggcgg tcgcgctac 
      atggccctg aaggagcgg agacagagg ctggcgcgc 
      agtcggagc agtcagcag tttggcgac gaagaggac 
      gtcgatgag cccatctcg ccgacgtcc tgcaacccg 
      tccgcgtac ttggcgtcg cgggggtac ggctccgca 
      gcatcgtca gggcacgat cttgcgaga agtcgctcc 
      tcgcatgcg ttgaagtcg cgcgataat tcgcctgat 
      aggtcgaac gcgacggag aaagatggg gccgcgttg 
      agttcttgg gcgaggtat ttaaagaac aagtacggg 
      aataggaac agtggcaaa gataaggaa tcgagtagt 
      tcggtgaca ccgtcgagt agttcgacg acggcgagg 
      agactgtct cttggtctc ccgctgagg tcgtccacg 
      gagttaggg agctcggac gacgaccaa aaaaacatg 
      caaggctcc cccactacc cctcctaca gcagctacg 
      gcggcagcc ggtatcgca gtggcagtg gcaggtacc 
      tcccctagg agtcagtac ttgcagaaa tgccaacag 
      ctgttcgaa attggtagt cgggggagc gaagccgga 
      tgtttcacc tggcctcgc ggcgttgcc gtgggcccc 
      gataactcc atcgtagtc gcggattcc tccaaccac 
      cgcgtccaa gtcttcgac tcaaacgga cgattcctg 
      aaagaattt ggacaatac ggcaacggc gaaggcgaa 
      ttcgactgt cttgcaggc gttgccgtc aacagaatt 
      ggccaattt atcatcgcc gaccggtac aaccaccga 
      atccaagtc ttcgaccct tcgggacgc ttcttgcga 
      gccttcggc agccagggc accgccgac ggccgcttc 
      aactacccg tggggcatc accaccgac gccctaggc 
      ttcatctac gtgtgtgac aaggaaaac cacagagtg 
      caggttttc cagtccgat ggtaccttc gtgggcaag 
      ttcggctcc atgggcagc aaggagggc caactggag 
      catccccac tacatcgcc gtttctaac acgaacagg 
      gtcgttgtt tcggactcg aacaaccac cgaatccag 
      atatttgac gtgaatggc aaagtcctg tcctccttc 
      gggtccgag ggctcggac gatggtcag ttcaagttc 
      cccagaggc gttgctgtg gatgaccaa ggctacatt 
      tgcgttgct gattccgga aataacaga atccagatt 
      ttccacccc gatggaaca tttttgcgt tcattcggt 
      tgttggggc atcggcaaa ggcgagttc aagggcctt 
      gaaggtgtc gcaatgacc cccaatgga cacatactg 
      gtgtgtgat cgagaaaac cacaggata caagtgttt 
      taaatcaaa ccaatttaa taatctaat acttttttc 
      attttaaca gtgtgttaa cgacttaca agcaataaa 
      tacttgcat ttctc
    • >TC031245 RB
      gacggcttt taccaaagt ttatgtgtg atgaattgt 
      gtatctgtg atgttttag cgaattaat gacaagact 
      gaaaaatgg aacaattcg agcaacttt taacatgtg 
      ccatttgtc tggaccgct accgcaacc ccaagctgt 
      tgccatgcc agcacagct tctgcatgg aaccatgtc 
      tggagggct tggtggatt acgtccgga gacaggtga 
      agtgtccgg aatgccgag cggaacacc gcatcccct 
      atcaggggg tgcagggtt tcccgacca acgtcaccc 
      ttcaaagat tcctggagc ttcatattg aaataactg 
      gcgaactcc cggatccaa caagtggcc aagttatgg 
      aacgatgta acgtttgct cagaaaagg cgtattgta 
      gtttttgtt cccactgtg ataagaaaa tctgtgaag 
      aatgcaagg gagcgcaca tggaaatcc tccggcggg 
      agatctccc ggattaaca accagatcc ggcgaggca 
      ttcataggt tacaagaca ctttgtctc tagtcgaga 
      aaaacaccc aaagtattc aatcgaact gctcgtcgg 
      tgagcgaag aagtcgagg agatttacc ggaggctct 
      ccaaagccc tcaaagacc ggacggaat atttacgag 
      gcgagatcg atcgctatt taggaacgg aattaagga 
      gtttgacca acttgaagg agaacttgg agcaagaga 
      tatccaaca ttttgagca actgcgacc tggccgaca 
      agcacatga acgagaacg tggacattt gggacgact 
      gcgagctca tggacgcca aagaaatct tcctcaaga 
      cagtcgaat tcatccgga acttcgaat acgaaaaca 
      cggattaca gcaggaaaa ttcggctgg ttttagcgc 
      acgacccaa atcaactcg tcctgcacg tcgccggct 
      acggcgatc taaacatag ccaatcagc actttagtc 
      aaggctcgg gcatgttgc aacctccag gaccgggct 
      taatgcggt ccaaaagcg accaccggc tagcctcac 
      agttcaggc agcaggagg aaagagggt atgagagag 
      gttacggcg gcgaccaag acgaggcgc tcctcggag 
      gccgcaaat tcggcgaaa ggcccaaaa cggagagat 
      acggtgaca gataccgtg gcactgact acggcgaag 
      actacgata acgactcca gaccggcta ggtctaggt 
      acagccgct tcaggaggc acggccaag ataacgact 
      ccgacgggg agcaaccgg gtcgtggag tcagattcg 
      aaccgcaga aggttgagc gggagaagg tcctggaca 
      cggaagacg tggccagag gccccttga gcggcatca 
      cccgcctgg ctgactcgc cccgcgtca tgaaaaagc 
      tccaagaga acgaaagcg gcaaaaaga aggaaaagg 
      aggctcctc caccacccg ccccccagc caaaagtcg 
      taccaaagc ggcccccgc cccccacaa acagacaag 
      taagcgaag aagacgaga tcgctaaaa taaagaaac 
      agaataaag gtgccacca cctccaccg aaacagaga 
      cccggccag agcgcaccg aagaaagac actcagctc 
      agagaaaaa cccccgccc ccgaggtga gtctagacg 
      agccgtaac ctcgcaaag tacctgatt aaaacaatt 
      tagtctaa
    • >TC031245 RC
      gacggcttt taccaaagt ttatgtgtg atgaattgt 
      gtatctgtg atgttttag cgaattaat gacaagact 
      gaaaaatgg aacaattcg agcaacttt taacatgtg 
      ccatttgtc tggaccgct accgcaacc ccaagctgt 
      tgccatgcc agcacagct tctgcatgg aaccatgtc 
      tggagggct tggtggatt acgtccgga gacaggtga 
      agtgtccgg aatgccgag cggaacacc gcatcccct 
      atcaggggg tgcagggtt tcccgacca acgtcaccc 
      ttcaaagat tcctggagc ttcatattg aaataactg 
      gcgaactcc cggatccaa caagtggcc aagttatgg 
      aacgatgta acgtttgct cagaaaagg cgtattgta 
      gtttttgtt cccactgtg ataagaaaa tctgtgaag 
      aatgcaagg gagcgcaca tggaaatcc tccggcggg 
      agatctccc ggattaaca accagatcc ggcgaggca 
      ttcataggt tacaagaca ctttgtctc tagtcgaga 
      aaaacaccc aaagtattc aatcgaact gctcgtcgg 
      tgagcgaag aagtcgagg agatttacc ggaggctct 
      ccaaagccc tcaaagacc ggacggaat atttacgag 
      gcgagatcg atcgctatt taggaacgg aattaagga 
      gtttgacca acttgaagg agaacttgg agcaagaga 
      tatccaaca ttttgagca actgcgacc tggccgaca 
      agcacatga acgagaacg tggacattt gggacgact 
      gcgagctca tggacgcca aagaaatct tcctcaaga 
      cagtcgaat tcatccgga acttcgaat acgaaaaca 
      cggattaca gcaggaaaa ttcggctgg ttttagcgc 
      acgacccaa atcaactcg tcctgcacg tcgccggct 
      acggcgatc taaacatag ccaatcagc actttagtc 
      aaggctcgg gcatgttgc aacctccag gaccgggct 
      taatgcggt ccaaaagcg accaccggc tagcctcac 
      agttcaggc agcaggagg aaagagggt atgagagag 
      gttacggcg gcgaccaag acgaggcgc tcctcggag 
      gccgcaaat tcggcgaaa ggcccaaaa cggagagat 
      acggtgaca gataccgtg gcactgact acggcgaag 
      actacgata acgactcca gaccggcta ggtctaggt 
      acagccgct tcaggaggc acggccaag ataacgact 
      ccgacgggg agcaaccgg gtcgtggag tcagattcg 
      aaccgcaga aggttgagc gggagaagg tcctggaca 
      cggaagacg tggccagag gccccttga gcggcatca 
      cccgcctgg ctgactcgc cccgcgtca tgaaaaagc 
      tccaagaga acgaaagcg gcaaaaaga aggaaaagg 
      aggctcctc caccacccg ccccccagc caaaagtcg 
      taccaaagc ggcccccgc cccccacaa acagacaag 
      taagcgaag aagacgaga tcgctaaaa taaagaaac 
      agaataaag gtgccacca cctccaccg aaacagaga 
      cccggccag agcgcaccg aagaaagac actcagctc 
      agagaaaaa cccccgccc ccgaggcca tctccagcc 
      ctgaagaat ccgacgaat ctctcactt ccttgcaac 
      aaacacagc gcaaaaccg ccgccagta aaacaggcg 
      cgagacgcg cctccgacg ctggacata aaaaatcca 
      cgcgatcgg cgagctccg actcgaaca cttccacag 
      aatccacag cttcctcaa acgccattc gcacaaccg 
      gggccccgt tcaccaccg aagaagtca aacagaagt 
      atctctcac gcgggagtg tcgacgcca ctccccgaa 
      ccccaacca aagaaccaa ccaaaccgt tccaaagcc 
      gcttcctcc acaacaaaa ccacgacac cgccatccg 
      ccaccaaag acgacgacg agaccgaaa ccagttcgg 
      aggaggaga cggaaagtg aggaggaat ccgacgagg 
      acaccaaaa aaccagaca ccaaagaaa tggcgaaga 
      ctgacattg gcccccttt tggcccgca gcgcgcacg 
      cgagggacg ggagcgccg aaaacgccc ggaaaagca 
      gccgggacg aaacctcca cgtacacca gacccaggt 
      acggggtcc cgaaggagg aatcgcccc cgcggtcgc 
      gctacgccc ggcgaacct caggggcgc aagacgagg 
      agcccccca ggtacggct acacgagcc gcttcctca 
      acaagagca aaagctcgg ctgccatcc cgcctgaag 
      aagacgacc acaagtaca gttcgacgc ttggcgacg 
      actccgaca gtaaatatg ggagcgggc ggtcgcgct 
      acatggccc tgaaggagc ggagacaga ggctggcgc 
      gcagtcgga gcagtcagc agtttggcg acgaagagg 
      acgtcgatg agcccatct cgccgacgt cctgcaacc 
      cgtccgcgt acttggcgt cgcgggggt acggctccg 
      cagcatcgt cagggcacg atcttgcga gaagtcgct 
      cctcgcatg cgttgaagt cgcgcgata attcgcctg 
      ataggtcga acgcgacgg agaaagatg gggccgcgt 
      tgagttctt gggcgaggt atttaaaga acaagtacg 
      ggaatagga acagtggca aagataagg aatcgagta 
      gttcggtga caccgtcga gtagttcga cgacggcga 
      ggagactgt ctcttggtc tcccgctga ggtcgtcca 
      cggagttag ggagctcgg acgacgacc aaaaaaaca 
      tgcaaggct cccccacta cccctccta cagcagcta 
      cggcggcag ccggtatcg cagtggcag tggcaggta 
      cctccccta ggagtcagt acttgcaga aatgccaac 
      agctgttcg aaattggta gtcggggga gcgaagccg 
      gatgtttca cctggcctc gcggcgttg ccgtgggcc 
      ccgataact ccatcgtag tcgcggatt cctccaacc 
      accgcgtcc aagtcttcg actcaaacg gacgattcc 
      tgaaagaat ttggacaat acggcaacg gcgaaggcg 
      aattcgact gtcttgcag gcgttgccg tcaacagaa 
      ttggccaat ttatcatcg ccgaccggt acaaccacc 
      gaatccaag tcttcgacc cttcgggac gcttcttgc 
      gagccttcg gcagccagg gcaccgccg acggccgct 
      tcaactacc cgtggggca tcaccaccg acgccctag 
      gcttcatct acgtgtgtg acaaggaaa accacagag 
      tgcaggttt tccagtccg atggtacct tcgtgggca 
      agttcggct ccatgggca gcaaggagg gccaactgg 
      agcatcccc actacatcg ccgtttcta acacgaaca 
      gggtcgttg tttcggact cgaacaacc accgaatcc 
      agatatttg acgtgaatg gcaaagtcc tgtcctcct 
      tcgggtccg agggctcgg acgatggtc agttcaagt 
      tccccagag gcgttgctg tggatgacc aaggctaca 
      tttgcgttg ctgattccg gaaataaca gaatccaga 
      ttttccacc ccgatggaa catttttgc gttcattcg 
      gttgttggg gcatcggca aaggcgagt tcaagggcc 
      ttgaaggtg tcgcaatga cccccaatg gacacatac 
      tggtgtgtg atcgagaaa accacagga tacaagtgt 
      tttaaatca aaccaattt aataatcta atacttttt 
      tcattttaa cagtgtgtt aacgactta caagcaata 
      aatacttgc atttctc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY