Status
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Gene information
General information
TC012512 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10741124..10759345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012512
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01985

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012512 RA
      MPEGPSDLE LRRRQFKAQ MSTVQTRRQ QAVCVRRAY 
      KRYGTKSNP YVILDGLNM TVPKGAIYG LLGASGCGK 
      TTLLNCIVG RKRLNSGEI WVLGGTPGS RGSGVPGPR 
      VGYMPQEIA LYGEFTIRE TLKYFGWIS GMTTQQVED 
      KLGFLVSFL MLPDADRLV KNLSGGQQR RVSLAATLI 
      HEPELLILD EPTVGVDPL LRQNIWNHL VEITKHGRT 
      TVIITTHYI DETRQAHLI GLMRGGYFL AEESPERLI 
      SQFGAESLE DVFLKLSVI QNMGKRRRS SILQGVTET 
      IQVPSGTIN EAAVIDDEV GEISGEFGD NVSMSSRGG 
      RVSIAPEPT ENAPPELPP EEEPPMTFA DYFKIVKLN 
      HMRALIWKN FLWMWRNAP VMAFIIGLP VVQTILFCL 
      SIGHDPTGL PLTVVNYEL DQSEVCKVT PGCNSTRLS 
      CNYLHFLKN RTLELSFYE NEEEGKEKV RHGKAWATL 
      VFPSNFSDS LRSRINGGR SVPDWDLVT SSVAVYQDK 
      SNENIGSYI QRDLYFAFE DFFKDYLSS CNIDTKVGT 
      IPIRWETPV YGYGNPDFT DFAAPGVIL TIIFFLAVA 
      LTSGAMLLE RNEGILERS LVNGISGIE LLSSHVITQ 
      FAVMFGQSA MVLIFSFYI FGITQNGNL LLVIILIVI 
      TGLCGMCFG FVVACSCDN ERSATYMAM GSFLPIVML 
      CGIIWPIEA MHSILQWIS YVLPLTKST ESLRCILAR 
      GWGIGTTEV YAGFVSTLI WIVVFLTIS ILLLKFKKG
    CDS sequence
    • >TC012512 RA
      atgccggaa ggaccaagc gatctggaa ctgcgcagg 
      aggcagttt aaagcccaa atgtccaca gtacaaact 
      cgtcgacag caggccgtt tgtgttcga cgagcttac 
      aaaagatac ggcaccaaa agcaacccc tacgttatt 
      ttagatggg ttgaacatg accgtgccg aaaggtgcc 
      atatatgga ttattaggg gccagtggt tgtggtaaa 
      acaactctt ctcaattgt atagttggt agaaaacga 
      ttaaattcg ggagaaata tgggtgttg ggtggcaca 
      ccaggatcc agaggaagc ggtgttcct ggaccacgt 
      gtaggatat atgccacag gaaatcgcc ctttatggc 
      gaattcaca atccgagag acgctcaaa tacttcggc 
      tggatctcc ggaatgacg acccagcaa gtcgaagac 
      aaactcgga tttctagtc agttttttg atgttgcca 
      gacgccgac cgcttggta aaaaacctg agcggtggt 
      cagcagaga cgcgtgtcc ttagctgcg acccttatt 
      catgaaccc gaactgctg atccttgac gagcccacg 
      gtgggcgta gatccgctg ttgcggcaa aacatttgg 
      aaccatttg gtcgaaatt accaaacac ggaaggaca 
      acggtcatt attacgacc cattacatc gacgaaacg 
      aggcaagcc catctgatc ggcctaatg cgcggtgga 
      tatttcctg gccgaggaa tcccccgaa cgcctcatc 
      tcccaattt ggggccgaa agcctcgaa gatgttttc 
      ctcaaactg agcgtcatc caaaacatg ggcaaaagg 
      cgtcgctct agcatcctc caaggagtc acagaaacg 
      atccaagtc cccagcgga acaatcaac gaagccgct 
      gtgatcgac gacgaagtc ggcgaaatt tccggagag 
      ttcggagac aacgtctcc atgtccagt cgagggggg 
      cgcgtctcc atagccccc gagcccacc gaaaacgcc 
      cctccggag ctcccccca gaggaagag cccccgatg 
      acgttcgcg gattatttc aaaatcgtc aaactgaac 
      cacatgagg gccctcatt tggaagaac ttcctctgg 
      atgtggaga aatgcgcct gttatggcc ttcataatc 
      ggactgcct gttgtccaa acaattctg ttttgtctg 
      tccattggg cacgacccc actggacta cccctaact 
      gtagttaac tacgaattg gaccagtct gaagtgtgt 
      aaagttaca ccgggttgt aacagtaca cggctgagt 
      tgtaactat ttgcacttt ttgaaaaac cggactttg 
      gaacttagt ttttacgaa aatgaagag gaaggtaaa 
      gagaaggtg cggcacggg aaagcttgg gcaacttta 
      gtgtttccg agtaatttt tcggattcg ttgaggtca 
      aggattaat ggaggtcga agtgtgccc gattgggat 
      ttggtcacg tcgtcggtt gcggtgtat caggacaag 
      tcaaatgaa aatatcggc agttatatt caaagagac 
      ttgtacttc gcttttgag gacttcttc aaagattac 
      ctaagttcc tgtaacatt gacacgaag gtcggaaca 
      ataccaatc agatgggaa acgccagtc tacggttac 
      ggcaatcca gacttcaca gattttgcg gcacctggt 
      gtcatactt acgattata ttcttcttg gcagtggct 
      ttgacctcc ggcgctatg ttactggaa cgaaacgaa 
      ggaatcctt gagcgatcc cttgttaat ggaatcagt 
      ggaatcgaa ctcctctct tcccacgtc atcactcaa 
      tttgcggtc atgtttggc caatctgcc atggtttta 
      attttctca ttttacatt tttggcatc acccaaaac 
      ggaaatctg cttctcgtc attatactt attgtaatc 
      actggcctt tgtggaatg tgttttgga tttgtcgtc 
      gcgtgcagt tgcgataac gaaagatcc gccacctat 
      atggccatg ggcagcttt ttacccatt gtgatgctg 
      tgtggcatc atatggcct atagaagct atgcatagc 
      atcctccaa tggatttcc tacgtgttg cctttgacg 
      aaatcgacg gagagcttg cggtgtatt cttgccagg 
      ggttggggt attggaacc accgaagtt tacgctgga 
      tttgtctca acactgatc tggattgtt gtcttctta 
      accattagt atactcttg ctcaaattt aagaagggt 
      tga
    mRNA sequence
    • >TC012512 RA
      ggggtcctc caggtgcag gcattcgtc gattagttg 
      cccatgtga ccatggact tccccactg ctgaacttg 
      gcgctcgtc attaaatct tcaaatgat tcaaaaaca 
      aaacattcg aattgttgt agagagtgg tcgctttgt 
      tagtgggtg ctagaaatt ttggaattt tcggactcg 
      tccagtaaa gaatcacaa tgccggaag gaccaagcg 
      atctggaac tgcgcagga ggcagttta aagcccaaa 
      tgtccacag tacaaactc gtcgacagc aggccgttt 
      gtgttcgac gagcttaca aaagatacg gcaccaaaa 
      gcaacccct acgttattt tagatgggt tgaacatga 
      ccgtgccga aaggtgcca tatatggat tattagggg 
      ccagtggtt gtggtaaaa caactcttc tcaattgta 
      tagttggta gaaaacgat taaattcgg gagaaatat 
      gggtgttgg gtggcacac caggatcca gaggaagcg 
      gtgttcctg gaccacgtg taggatata tgccacagg 
      aaatcgccc tttatggcg aattcacaa tccgagaga 
      cgctcaaat acttcggct ggatctccg gaatgacga 
      cccagcaag tcgaagaca aactcggat ttctagtca 
      gttttttga tgttgccag acgccgacc gcttggtaa 
      aaaacctga gcggtggtc agcagagac gcgtgtcct 
      tagctgcga cccttattc atgaacccg aactgctga 
      tccttgacg agcccacgg tgggcgtag atccgctgt 
      tgcggcaaa acatttgga accatttgg tcgaaatta 
      ccaaacacg gaaggacaa cggtcatta ttacgaccc 
      attacatcg acgaaacga ggcaagccc atctgatcg 
      gcctaatgc gcggtggat atttcctgg ccgaggaat 
      cccccgaac gcctcatct cccaatttg gggccgaaa 
      gcctcgaag atgttttcc tcaaactga gcgtcatcc 
      aaaacatgg gcaaaaggc gtcgctcta gcatcctcc 
      aaggagtca cagaaacga tccaagtcc ccagcggaa 
      caatcaacg aagccgctg tgatcgacg acgaagtcg 
      gcgaaattt ccggagagt tcggagaca acgtctcca 
      tgtccagtc gaggggggc gcgtctcca tagcccccg 
      agcccaccg aaaacgccc ctccggagc tccccccag 
      aggaagagc ccccgatga cgttcgcgg attatttca 
      aaatcgtca aactgaacc acatgaggg ccctcattt 
      ggaagaact tcctctgga tgtggagaa atgcgcctg 
      ttatggcct tcataatcg gactgcctg ttgtccaaa 
      caattctgt tttgtctgt ccattgggc acgacccca 
      ctggactac ccctaactg tagttaact acgaattgg 
      accagtctg aagtgtgta aagttacac cgggttgta 
      acagtacac ggctgagtt gtaactatt tgcactttt 
      tgaaaaacc ggactttgg aacttagtt tttacgaaa 
      atgaagagg aaggtaaag agaaggtgc ggcacggga 
      aagcttggg caactttag tgtttccga gtaattttt 
      cggattcgt tgaggtcaa ggattaatg gaggtcgaa 
      gtgtgcccg attgggatt tggtcacgt cgtcggttg 
      cggtgtatc aggacaagt caaatgaaa atatcggca 
      gttatattc aaagagact tgtacttcg cttttgagg 
      acttcttca aagattacc taagttcct gtaacattg 
      acacgaagg tcggaacaa taccaatca gatgggaaa 
      cgccagtct acggttacg gcaatccag acttcacag 
      attttgcgg cacctggtg tcatactta cgattatat 
      tcttcttgg cagtggctt tgacctccg gcgctatgt 
      tactggaac gaaacgaag gaatccttg agcgatccc 
      ttgttaatg gaatcagtg gaatcgaac tcctctctt 
      cccacgtca tcactcaat ttgcggtca tgtttggcc 
      aatctgcca tggttttaa ttttctcat tttacattt 
      ttggcatca cccaaaacg gaaatctgc ttctcgtca 
      ttatactta ttgtaatca ctggccttt gtggaatgt 
      gttttggat ttgtcgtcg cgtgcagtt gcgataacg 
      aaagatccg ccacctata tggccatgg gcagctttt 
      tacccattg tgatgctgt gtggcatca tatggccta 
      tagaagcta tgcatagca tcctccaat ggatttcct 
      acgtgttgc ctttgacga aatcgacgg agagcttgc 
      ggtgtattc ttgccaggg gttggggta ttggaacca 
      ccgaagttt acgctggat ttgtctcaa cactgatct 
      ggattgttg tcttcttaa ccattagta tactcttgc 
      tcaaattta agaagggtt gattgaaat atttttgtg 
      atgagtcta gtgtatcga gtagtttta tatttgtat 
      taatttaat ttgttttta tttaaataa aacgacttt 
      cgtatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY