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Gene information
General information
TC012481 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 1275488..1281662
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012481
    iBeetle screen iB_01983
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages random partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012481 RA
      MFDHSIRVP RLYKTASKI AKEVSSGKG SIKQLVYEC 
      KHPNIKALY ALIVHTFQK SAHIEKLIK KSKLLIKEP 
      RFDPWLAKV LITELLWGK QRLSGQSKP VQTILAYEQ 
      VLKAHLSDV SDDVEEVEQ VEKPRYVRV NTLKITVNE 
      AIEGFREEG WVLKRYTDS ENYLGFLEA VSNLGSNEF 
      MVDLHIPYL LIFPPKTEF YQHAAYKNG SIILQDKAS 
      CLPVHILDP QPGTSILDM CAAPGMKTT QCAALIDNI 
      GKIYAVEIG TKRFHTLEK IVESSGASC VEPINSDVL 
      QLTAQQYPD IDYILVDPS CSGSGLTYR VETGQQAQD 
      QHTERIERL AGFQIKILR HALSKFPKV KRVVYSTCS 
      VYAEENEDV VRQVLETCN RFKLVPADQ FLGKKWYNF 
      GSSEFGDLG KFCLYAKPD IDLTSGFFV AVFERLEDG 
      ETNQFLNAQ LFEEKMRIV SLLNKGKTE NLQEEKQMV 
      EETDQKRHI KEETEEVKD MNVKSETSD EGKKSKKKK 
      KHKSAEVTD SLDIKKETG DGFVKKKKL DNKEEILKI 
      KKEESEINK NVDIKTETK SKKKKNRDV DASENQNEA 
      ESKLDIKGE LDEGRNGKK KKKKHKHSG ESLETKKEE 
      SEPREDLNV ATETDDGEG SRKLKRKKK HRGVKTENC 
      EEMKEVIIK RETNDDKAI TSKKKKKLK KPDDNVTIE 
      NTCEEDIKI KTERGEKSR KKHKDVNKL DADGEFFDQ 
      KKKKGDESM VKPEENSDE SVPKKKKKK KDRVDENRG 
      GSDNNFFDA FD
    CDS sequence
    • >TC012481 RA
      atgtttgac cattcaatt agagtgccc cgattgtac 
      aaaactgcc tcaaaaatc gcaaaggaa gtgagttcg 
      ggtaaaggc agcatcaaa cagctcgtc tacgaatgt 
      aaacatccc aatattaaa gctttgtac gcgttaatt 
      gttcacacg tttcaaaag tctgcccat attgagaaa 
      ttaatcaaa aaatcaaaa cttttaatc aaagagccg 
      agatttgac ccctggttg gcgaaagtc ctaatcact 
      gagttgttg tggggtaaa caacggctc tcagggcag 
      tctaagccg gtccaaacg attttggcg tatgaacaa 
      gttttgaaa gcgcacctg agcgatgtt tctgatgat 
      gtggaggaa gtagagcaa gttgaaaaa ccgcgatac 
      gtccgcgtg aatactctc aaaatcacc gtaaacgaa 
      gctattgag ggctttcga gaagaaggt tgggtttta 
      aaaagatac acagacagt gagaattac ttgggtttt 
      ctggaagct gtgtcaaac ctaggctct aacgaattc 
      atggttgat ttacacatt ccgtattta ctaattttc 
      ccaccgaaa accgagttt taccaacat gctgcgtac 
      aaaaacggc agtataatt ttacaagac aaggccagt 
      tgtcttcct gtgcatatt ctcgatcct caacctggg 
      acatcaatt ctggacatg tgcgcagca cctgggatg 
      aaaaccact cagtgtgct gctcttatc gataatata 
      gggaaaatc tatgccgtt gaaatcggc acaaagagg 
      ttccacact ttggaaaaa attgtcgaa tcttcgggg 
      gcttcgtgt gtggagccg attaactcc gatgtgtta 
      caactaacc gcacaacag taccccgat attgactat 
      attttagta gaccccagt tgttcaggc tcaggttta 
      acctatcga gtcgaaacc ggacaacag gcccaagac 
      caacacaca gagcgcata gagcggttg gctggtttc 
      caaattaaa attctgaga catgcgttg tctaaattt 
      cccaaagtt aaacgagtc gtgtattcg acttgttcc 
      gtatatgca gaagaaaac gaagacgtc gtcaggcaa 
      gtcttagag acttgcaat aggtttaaa ctggttcct 
      gctgaccag tttttgggt aaaaaatgg tacaatttt 
      ggttcgtcc gaatttggg gatttgggg aaattttgt 
      ttatatgcc aaacccgac atagacctg actagtgga 
      tttttcgtt gctgtgttt gaaagacta gaagatggc 
      gaaactaat cagtttttg aatgcgcaa ttatttgaa 
      gaaaaaatg cgcattgta agtttgctg aataaaggg 
      aaaactgaa aatctgcaa gaggagaaa caaatggtt 
      gaagaaact gaccaaaaa agacacata aaagaagag 
      acggaagaa gtaaaagat atgaacgta aaaagtgaa 
      actagtgat gaaggtaaa aaaagtaag aagaaaaag 
      aaacataaa agtgctgaa gttaccgac agtctggac 
      attaaaaaa gaaacgggt gatggtttt gttaaaaag 
      aagaaactt gacaacaaa gaagaaatc ttaaaaatc 
      aagaaagaa gaaagtgaa attaacaag aatgttgac 
      attaaaacg gagacaaaa tctaaaaag aagaaaaat 
      cgtgatgtt gatgcttcg gaaaatcag aatgaagct 
      gaaagtaaa ctggatatt aaaggagaa ttagatgag 
      ggtagaaat ggcaaaaag aagaagaag aaacataaa 
      cacagtggt gaaagtttg gaaaccaag aaagaagaa 
      tccgaacca agagaagat ctgaacgtg gccaccgaa 
      acagacgat ggtgaaggg tctagaaag ttaaaaagg 
      aagaaaaaa catagggga gttaagaca gaaaactgt 
      gaagaaatg aaagaagtg atcataaaa agagaaaca 
      aatgatgat aaagctata acgtcaaaa aagaagaag 
      aaactgaaa aagccggac gataatgta actatagaa 
      aatacctgt gaagaagat ataaaaatt aaaacagaa 
      agaggtgaa aaaagtaga aaaaagcat aaagatgtt 
      aacaagttg gacgctgat ggtgaattt tttgaccaa 
      aagaagaag aagggagat gaatctatg gttaaaccc 
      gaggaaaac agcgatgaa agtgtacca aaaaagaag 
      aaaaagaag aaagacaga gttgatgaa aaccgggga 
      ggaagtgac aataatttt tttgacgct tttgattaa
    mRNA sequence
    • >TC012481 RA
      tgcatttat tttactttt ctgttgtta actcggttt 
      agttgcact ttttgcaat gtattttgt aacgaaaac 
      gtttcaatt ttccaaatt gaccgtttg gtaactttc 
      ggcccggtt ggcagcgcg taaaaacgt gacgtgggg 
      ttgtattcc aagaaaagc tccaggaaa acgtttttc 
      acaggtaaa aataatgtt tgaccattc aattagagt 
      gccccgatt gtacaaaac tgcctcaaa aatcgcaaa 
      ggaagtgag ttcgggtaa aggcagcat caaacagct 
      cgtctacga atgtaaaca tcccaatat taaagcttt 
      gtacgcgtt aattgttca cacgtttca aaagtctgc 
      ccatattga gaaattaat caaaaaatc aaaactttt 
      aatcaaaga gccgagatt tgacccctg gttggcgaa 
      agtcctaat cactgagtt gttgtgggg taaacaacg 
      gctctcagg gcagtctaa gccggtcca aacgatttt 
      ggcgtatga acaagtttt gaaagcgca cctgagcga 
      tgtttctga tgatgtgga ggaagtaga gcaagttga 
      aaaaccgcg atacgtccg cgtgaatac tctcaaaat 
      caccgtaaa cgaagctat tgagggctt tcgagaaga 
      aggttgggt tttaaaaag atacacaga cagtgagaa 
      ttacttggg ttttctgga agctgtgtc aaacctagg 
      ctctaacga attcatggt tgatttaca cattccgta 
      tttactaat tttcccacc gaaaaccga gttttacca 
      acatgctgc gtacaaaaa cggcagtat aattttaca 
      agacaaggc cagttgtct tcctgtgca tattctcga 
      tcctcaacc tgggacatc aattctgga catgtgcgc 
      agcacctgg gatgaaaac cactcagtg tgctgctct 
      tatcgataa tatagggaa aatctatgc cgttgaaat 
      cggcacaaa gaggttcca cactttgga aaaaattgt 
      cgaatcttc gggggcttc gtgtgtgga gccgattaa 
      ctccgatgt gttacaact aaccgcaca acagtaccc 
      cgatattga ctatatttt agtagaccc cagttgttc 
      aggctcagg tttaaccta tcgagtcga aaccggaca 
      acaggccca agaccaaca cacagagcg catagagcg 
      gttggctgg tttccaaat taaaattct gagacatgc 
      gttgtctaa atttcccaa agttaaacg agtcgtgta 
      ttcgacttg ttccgtata tgcagaaga aaacgaaga 
      cgtcgtcag gcaagtctt agagacttg caataggtt 
      taaactggt tcctgctga ccagttttt gggtaaaaa 
      atggtacaa ttttggttc gtccgaatt tggggattt 
      ggggaaatt ttgtttata tgccaaacc cgacataga 
      cctgactag tggattttt cgttgctgt gtttgaaag 
      actagaaga tggcgaaac taatcagtt tttgaatgc 
      gcaattatt tgaagaaaa aatgcgcat tgtaagttt 
      gctgaataa agggaaaac tgaaaatct gcaagagga 
      gaaacaaat ggttgaaga aactgacca aaaaagaca 
      cataaaaga agagacgga agaagtaaa agatatgaa 
      cgtaaaaag tgaaactag tgatgaagg taaaaaaag 
      taagaagaa aaagaaaca taaaagtgc tgaagttac 
      cgacagtct ggacattaa aaaagaaac gggtgatgg 
      ttttgttaa aaagaagaa acttgacaa caaagaaga 
      aatcttaaa aatcaagaa agaagaaag tgaaattaa 
      caagaatgt tgacattaa aacggagac aaaatctaa 
      aaagaagaa aaatcgtga tgttgatgc ttcggaaaa 
      tcagaatga agctgaaag taaactgga tattaaagg 
      agaattaga tgagggtag aaatggcaa aaagaagaa 
      gaagaaaca taaacacag tggtgaaag tttggaaac 
      caagaaaga agaatccga accaagaga agatctgaa 
      cgtggccac cgaaacaga cgatggtga agggtctag 
      aaagttaaa aaggaagaa aaaacatag gggagttaa 
      gacagaaaa ctgtgaaga aatgaaaga agtgatcat 
      aaaaagaga aacaaatga tgataaagc tataacgtc 
      aaaaaagaa gaagaaact gaaaaagcc ggacgataa 
      tgtaactat agaaaatac ctgtgaaga agatataaa 
      aattaaaac agaaagagg tgaaaaaag tagaaaaaa 
      gcataaaga tgttaacaa gttggacgc tgatggtga 
      attttttga ccaaaagaa gaagaaggg agatgaatc 
      tatggttaa acccgagga aaacagcga tgaaagtgt 
      accaaaaaa gaagaaaaa gaagaaaga cagagttga 
      tgaaaaccg gggaggaag tgacaataa tttttttga 
      cgcttttga ttaaacaat gttccactt tggaagaag 
      tttgtgaat tagtttttt attattttg tttttgcta 
      ataaaatgt tccattttg a