General information | |||
TC012481 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 9079931..9086105 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012481 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01983 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
head appendages random partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC012481 RA
MFDHSIRVP RLYKTASKI AKEVSSGKG SIKQLVYEC KHPNIKALY ALIVHTFQK SAHIEKLIK KSKLLIKEP RFDPWLAKV LITELLWGK QRLSGQSKP VQTILAYEQ VLKAHLSDV SDDVEEVEQ VEKPRYVRV NTLKITVNE AIEGFREEG WVLKRYTDS ENYLGFLEA VSNLGSNEF MVDLHIPYL LIFPPKTEF YQHAAYKNG SIILQDKAS CLPVHILDP QPGTSILDM CAAPGMKTT QCAALIDNI GKIYAVEIG TKRFHTLEK IVESSGASC VEPINSDVL QLTAQQYPD IDYILVDPS CSGSGLTYR VETGQQAQD QHTERIERL AGFQIKILR HALSKFPKV KRVVYSTCS VYAEENEDV VRQVLETCN RFKLVPADQ FLGKKWYNF GSSEFGDLG KFCLYAKPD IDLTSGFFV AVFERLEDG ETNQFLNAQ LFEEKMRIV SLLNKGKTE NLQEEKQMV EETDQKRHI KEETEEVKD MNVKSETSD EGKKSKKKK KHKSAEVTD SLDIKKETG DGFVKKKKL DNKEEILKI KKEESEINK NVDIKTETK SKKKKNRDV DASENQNEA ESKLDIKGE LDEGRNGKK KKKKHKHSG ESLETKKEE SEPREDLNV ATETDDGEG SRKLKRKKK HRGVKTENC EEMKEVIIK RETNDDKAI TSKKKKKLK KPDDNVTIE NTCEEDIKI KTERGEKSR KKHKDVNKL DADGEFFDQ KKKKGDESM VKPEENSDE SVPKKKKKK KDRVDENRG GSDNNFFDA FD
- >TC012481 RA
atgtttgac cattcaatt agagtgccc cgattgtac aaaactgcc tcaaaaatc gcaaaggaa gtgagttcg ggtaaaggc agcatcaaa cagctcgtc tacgaatgt aaacatccc aatattaaa gctttgtac gcgttaatt gttcacacg tttcaaaag tctgcccat attgagaaa ttaatcaaa aaatcaaaa cttttaatc aaagagccg agatttgac ccctggttg gcgaaagtc ctaatcact gagttgttg tggggtaaa caacggctc tcagggcag tctaagccg gtccaaacg attttggcg tatgaacaa gttttgaaa gcgcacctg agcgatgtt tctgatgat gtggaggaa gtagagcaa gttgaaaaa ccgcgatac gtccgcgtg aatactctc aaaatcacc gtaaacgaa gctattgag ggctttcga gaagaaggt tgggtttta aaaagatac acagacagt gagaattac ttgggtttt ctggaagct gtgtcaaac ctaggctct aacgaattc atggttgat ttacacatt ccgtattta ctaattttc ccaccgaaa accgagttt taccaacat gctgcgtac aaaaacggc agtataatt ttacaagac aaggccagt tgtcttcct gtgcatatt ctcgatcct caacctggg acatcaatt ctggacatg tgcgcagca cctgggatg aaaaccact cagtgtgct gctcttatc gataatata gggaaaatc tatgccgtt gaaatcggc acaaagagg ttccacact ttggaaaaa attgtcgaa tcttcgggg gcttcgtgt gtggagccg attaactcc gatgtgtta caactaacc gcacaacag taccccgat attgactat attttagta gaccccagt tgttcaggc tcaggttta acctatcga gtcgaaacc ggacaacag gcccaagac caacacaca gagcgcata gagcggttg gctggtttc caaattaaa attctgaga catgcgttg tctaaattt cccaaagtt aaacgagtc gtgtattcg acttgttcc gtatatgca gaagaaaac gaagacgtc gtcaggcaa gtcttagag acttgcaat aggtttaaa ctggttcct gctgaccag tttttgggt aaaaaatgg tacaatttt ggttcgtcc gaatttggg gatttgggg aaattttgt ttatatgcc aaacccgac atagacctg actagtgga tttttcgtt gctgtgttt gaaagacta gaagatggc gaaactaat cagtttttg aatgcgcaa ttatttgaa gaaaaaatg cgcattgta agtttgctg aataaaggg aaaactgaa aatctgcaa gaggagaaa caaatggtt gaagaaact gaccaaaaa agacacata aaagaagag acggaagaa gtaaaagat atgaacgta aaaagtgaa actagtgat gaaggtaaa aaaagtaag aagaaaaag aaacataaa agtgctgaa gttaccgac agtctggac attaaaaaa gaaacgggt gatggtttt gttaaaaag aagaaactt gacaacaaa gaagaaatc ttaaaaatc aagaaagaa gaaagtgaa attaacaag aatgttgac attaaaacg gagacaaaa tctaaaaag aagaaaaat cgtgatgtt gatgcttcg gaaaatcag aatgaagct gaaagtaaa ctggatatt aaaggagaa ttagatgag ggtagaaat ggcaaaaag aagaagaag aaacataaa cacagtggt gaaagtttg gaaaccaag aaagaagaa tccgaacca agagaagat ctgaacgtg gccaccgaa acagacgat ggtgaaggg tctagaaag ttaaaaagg aagaaaaaa catagggga gttaagaca gaaaactgt gaagaaatg aaagaagtg atcataaaa agagaaaca aatgatgat aaagctata acgtcaaaa aagaagaag aaactgaaa aagccggac gataatgta actatagaa aatacctgt gaagaagat ataaaaatt aaaacagaa agaggtgaa aaaagtaga aaaaagcat aaagatgtt aacaagttg gacgctgat ggtgaattt tttgaccaa aagaagaag aagggagat gaatctatg gttaaaccc gaggaaaac agcgatgaa agtgtacca aaaaagaag aaaaagaag aaagacaga gttgatgaa aaccgggga ggaagtgac aataatttt tttgacgct tttgattaa
- >TC012481 RA
tgcatttat tttactttt ctgttgtta actcggttt agttgcact ttttgcaat gtattttgt aacgaaaac gtttcaatt ttccaaatt gaccgtttg gtaactttc ggcccggtt ggcagcgcg taaaaacgt gacgtgggg ttgtattcc aagaaaagc tccaggaaa acgtttttc acaggtaaa aataatgtt tgaccattc aattagagt gccccgatt gtacaaaac tgcctcaaa aatcgcaaa ggaagtgag ttcgggtaa aggcagcat caaacagct cgtctacga atgtaaaca tcccaatat taaagcttt gtacgcgtt aattgttca cacgtttca aaagtctgc ccatattga gaaattaat caaaaaatc aaaactttt aatcaaaga gccgagatt tgacccctg gttggcgaa agtcctaat cactgagtt gttgtgggg taaacaacg gctctcagg gcagtctaa gccggtcca aacgatttt ggcgtatga acaagtttt gaaagcgca cctgagcga tgtttctga tgatgtgga ggaagtaga gcaagttga aaaaccgcg atacgtccg cgtgaatac tctcaaaat caccgtaaa cgaagctat tgagggctt tcgagaaga aggttgggt tttaaaaag atacacaga cagtgagaa ttacttggg ttttctgga agctgtgtc aaacctagg ctctaacga attcatggt tgatttaca cattccgta tttactaat tttcccacc gaaaaccga gttttacca acatgctgc gtacaaaaa cggcagtat aattttaca agacaaggc cagttgtct tcctgtgca tattctcga tcctcaacc tgggacatc aattctgga catgtgcgc agcacctgg gatgaaaac cactcagtg tgctgctct tatcgataa tatagggaa aatctatgc cgttgaaat cggcacaaa gaggttcca cactttgga aaaaattgt cgaatcttc gggggcttc gtgtgtgga gccgattaa ctccgatgt gttacaact aaccgcaca acagtaccc cgatattga ctatatttt agtagaccc cagttgttc aggctcagg tttaaccta tcgagtcga aaccggaca acaggccca agaccaaca cacagagcg catagagcg gttggctgg tttccaaat taaaattct gagacatgc gttgtctaa atttcccaa agttaaacg agtcgtgta ttcgacttg ttccgtata tgcagaaga aaacgaaga cgtcgtcag gcaagtctt agagacttg caataggtt taaactggt tcctgctga ccagttttt gggtaaaaa atggtacaa ttttggttc gtccgaatt tggggattt ggggaaatt ttgtttata tgccaaacc cgacataga cctgactag tggattttt cgttgctgt gtttgaaag actagaaga tggcgaaac taatcagtt tttgaatgc gcaattatt tgaagaaaa aatgcgcat tgtaagttt gctgaataa agggaaaac tgaaaatct gcaagagga gaaacaaat ggttgaaga aactgacca aaaaagaca cataaaaga agagacgga agaagtaaa agatatgaa cgtaaaaag tgaaactag tgatgaagg taaaaaaag taagaagaa aaagaaaca taaaagtgc tgaagttac cgacagtct ggacattaa aaaagaaac gggtgatgg ttttgttaa aaagaagaa acttgacaa caaagaaga aatcttaaa aatcaagaa agaagaaag tgaaattaa caagaatgt tgacattaa aacggagac aaaatctaa aaagaagaa aaatcgtga tgttgatgc ttcggaaaa tcagaatga agctgaaag taaactgga tattaaagg agaattaga tgagggtag aaatggcaa aaagaagaa gaagaaaca taaacacag tggtgaaag tttggaaac caagaaaga agaatccga accaagaga agatctgaa cgtggccac cgaaacaga cgatggtga agggtctag aaagttaaa aaggaagaa aaaacatag gggagttaa gacagaaaa ctgtgaaga aatgaaaga agtgatcat aaaaagaga aacaaatga tgataaagc tataacgtc aaaaaagaa gaagaaact gaaaaagcc ggacgataa tgtaactat agaaaatac ctgtgaaga agatataaa aattaaaac agaaagagg tgaaaaaag tagaaaaaa gcataaaga tgttaacaa gttggacgc tgatggtga attttttga ccaaaagaa gaagaaggg agatgaatc tatggttaa acccgagga aaacagcga tgaaagtgt accaaaaaa gaagaaaaa gaagaaaga cagagttga tgaaaaccg gggaggaag tgacaataa tttttttga cgcttttga ttaaacaat gttccactt tggaagaag tttgtgaat tagtttttt attattttg tttttgcta ataaaatgt tccattttg a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
