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Gene information
General information
TC012466 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 673808..678884
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012466
    iBeetle screen iB_01982
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    head slightly orientation irregular - Number of animals showing the phenotype: 10

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012466 RA
      MATINFLLV ILVITSPSL QDLANNQID DDVNPFAEA 
      KAEILKEQN LQNIGGMIN NLMQSDGAK QLGDILTNA 
      ASGENAGEI LQGLGSVLG QSKGLDPSM LSMLFNMFQ 
      QGGTRSDDS FDMNALASL LGSFMGQSD GEKANIWTY 
      IPMILQTAN AFLGPEAEA RARNHQDHA NLVPPLLEK 
      LHLLFDHFI NSEMGRKMM NTVGAEKFV KVFADENGR 
      MSYRRFVDM LENHSFRKH WIRMLTNRI ASIISHFSD 
      PKTQKKYLT TFQHFINSL IKSQGYPKA ALFDPSRPT 
      ETITALANH FAKETLHMK ISSKQYVKP FVEYVQELF 
      RLAQKKGML SVDSHQLSD KLADTINLE VIEPIARVN 
      RAYRFAKKV PQCDRYVMC LVNQESQDE KPSLPGLRP 
      ILSKSASLI LSWFLSSTT KTPYLSLYM AVMDNKDCK 
      TWYQDACND FHHEEIKVK RELVHSEL
    CDS sequence
    • >TC012466 RA
      atggctacc atcaatttc cttctagtc atcctagtt 
      atcacaagt ccctctctc caggacctc gccaacaat 
      caaatcgac gatgatgtc aatcccttc gccgaagct 
      aaagccgag atcctcaaa gagcaaaac ctgcaaaac 
      atcggcgga atgatcaac aacttgatg caatcggac 
      ggtgccaaa caactcggc gatatcctg actaacgcc 
      gcttccggc gaaaacgcc ggtgaaatc ctccaaggt 
      ctgggctca gtcttgggt cagagtaaa ggcctcgac 
      ccatcaatg ctctcaatg ctcttcaac atgttccaa 
      caaggtggt accagaagt gacgattcc ttcgacatg 
      aacgcctta gcctccctt ctgggtagc ttcatgggg 
      caatcggac ggcgaaaag gccaacatc tggacctac 
      atccccatg atcctccaa accgccaac gcgttcctg 
      gggcccgag gccgaagcc cgcgccaga aaccaccaa 
      gaccacgca aaccttgtg cccccgctc ctggagaag 
      ctgcacctc ctttttgac cacttcatc aactcggaa 
      atgggccgg aagatgatg aacaccgtc ggggccgag 
      aagttcgtc aaagtgttc gccgacgaa aacgggcga 
      atgtcgtac cgacgcttc gtcgatatg ttggagaat 
      cattcgttt agaaaacac tggattcgg atgctcacc 
      aaccgaatc gcctcaatt atctcgcac ttttccgac 
      cccaaaacc cagaagaag tatctaacg acgttccag 
      cactttatc aacagtctt atcaagagt caagggtac 
      cccaaggcc gcgcttttt gatccaagc cgaccaact 
      gaaaccatc acagccctg gctaaccat tttgccaaa 
      gagacgctt cacatgaag atcagctcg aagcagtac 
      gtgaaaccg ttcgtggaa tacgtgcag gaactgttc 
      cgtcttgcc cagaagaag gggatgttg agcgtggat 
      tcgcaccaa ctctccgac aagttggcc gacaccatc 
      aacctcgag gtgatagaa cccatcgca agggtcaat 
      cgagcgtac agatttgcg aagaaagta ccacaatgt 
      gacaggtac gtgatgtgt ctggtcaat caggaatcg 
      caagatgag aaaccaagt cttcccggt ctaagacca 
      atcttgagc aaatcagcg agtcttatt ctgagctgg 
      ttcctgagc agcaccact aaaaccccc tacttgagc 
      ctctacatg gccgtgatg gacaacaaa gattgcaag 
      acttggtac caagacgcc tgtaatgac ttccaccac 
      gaagaaatc aaagtcaag cgggagttg gtgcatagt 
      gaactataa
    mRNA sequence
    • >TC012466 RA
      ggaatgttc caggtaagc atacgacct actcaacaa 
      tcttaaaag gtgtattac gagtggcag gtagtcagt 
      cagtgttga atcgaaacc agaaccgtt tcgaggtac 
      cgtaagtac agttacaaa atcatctac gtcaggccg 
      gaaagtacc atcgagttc tgtcacttg tggtttgat 
      tgcagccgc gatgtgtga catgtgaat ggcccaaca 
      tttaaaaaa gtaattact aacgcgagc acttttaca 
      agattagta ccaatggct accatcaat ttccttcta 
      gtcatccta gttatcaca agtccctct ctccaggac 
      ctcgccaac aatcaaatc gacgatgat gtcaatccc 
      ttcgccgaa gctaaagcc gagatcctc aaagagcaa 
      aacctgcaa aacatcggc ggaatgatc aacaacttg 
      atgcaatcg gacggtgcc aaacaactc ggcgatatc 
      ctgactaac gccgcttcc ggcgaaaac gccggtgaa 
      atcctccaa ggtctgggc tcagtcttg ggtcagagt 
      aaaggcctc gacccatca atgctctca atgctcttc 
      aacatgttc caacaaggt ggtaccaga agtgacgat 
      tccttcgac atgaacgcc ttagcctcc cttctgggt 
      agcttcatg gggcaatcg gacggcgaa aaggccaac 
      atctggacc tacatcccc atgatcctc caaaccgcc 
      aacgcgttc ctggggccc gaggccgaa gcccgcgcc 
      agaaaccac caagaccac gcaaacctt gtgcccccg 
      ctcctggag aagctgcac ctccttttt gaccacttc 
      atcaactcg gaaatgggc cggaagatg atgaacacc 
      gtcggggcc gagaagttc gtcaaagtg ttcgccgac 
      gaaaacggg cgaatgtcg taccgacgc ttcgtcgat 
      atgttggag aatcattcg tttagaaaa cactggatt 
      cggatgctc accaaccga atcgcctca attatctcg 
      cacttttcc gaccccaaa acccagaag aagtatcta 
      acgacgttc cagcacttt atcaacagt cttatcaag 
      agtcaaggg taccccaag gccgcgctt tttgatcca 
      agccgacca actgaaacc atcacagcc ctggctaac 
      cattttgcc aaagagacg cttcacatg aagatcagc 
      tcgaagcag tacgtgaaa ccgttcgtg gaatacgtg 
      caggaactg ttccgtctt gcccagaag aaggggatg 
      ttgagcgtg gattcgcac caactctcc gacaagttg 
      gccgacacc atcaacctc gaggtgata gaacccatc 
      gcaagggtc aatcgagcg tacagattt gcgaagaaa 
      gtaccacaa tgtgacagg tacgtgatg tgtctggtc 
      aatcaggaa tcgcaagat gagaaacca agtcttccc 
      ggtctaaga ccaatcttg agcaaatca gcgagtctt 
      attctgagc tggttcctg agcagcacc actaaaacc 
      ccctacttg agcctctac atggccgtg atggacaac 
      aaagattgc aagacttgg taccaagac gcctgtaat 
      gacttccac cacgaagaa atcaaagtc aagcgggag 
      ttggtgcat agtgaacta taataagta gactgagtg 
      tttgacgct cttttgggc ataactttg ttaaacaaa 
      ttgtattta tttgtactt gtaaattat taaaattaa 
      tgaaaaagt