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Gene information
General information
TC012458 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8360358..8366403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012458
    iBeetle screen iB_01981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012458 RA
      MHCDGAIRN IEKEILREC AYKGIQVHE NFLNYYLKL 
LSLDPTWGI TGKDVLDRS DVQNFIKYV VHQLENQNT 
PSMITLKMQ FYFTCNFQM KQKMVKRNR TELLMRLRG 
LEQEIIDVK YLTEEDEIN LMYKKIVYY ITLASGLGN 
PTFDAVFKE VRVALGSIL DRNELNDFV AQPRFTKID 
NLAEFTKLV TGIRLFNKD CGKGGEGME DLPKLINEA 
LIATNAELQ NTLISIMDK VNLMTTALD FVCGTEMDE 
RHNYVIAYR LPKGVTMAD VNYVKDSLI AYRQHELYV 
RKMMDEIDR IESTSAQIF TILQRALID LHRVVKHRI 
AIPVNQVFP HFIKLSDIW CNLQDQTIL LAKHNQIMM 
NLQKYIKVL SFPDSIAEM MIPPGESPM TDTERLQKT 
ADQKIPQED NHSMVLDPL LYTDLNKLK YEYLGFCAW 
KIFMTEGAL LPGNPGLGI AKTMNMQFV FSSVEAGIA 
FGKCPEKCV METIKLARR KPELINLLA IKDRLILVQ 
NIRDLVHEP PLLPLTDEV EVQTEMHPV ESNIDRNYF 
WNVWDLRRK AIEYANLTH CQTVSCQTK KSYYRFNSK 
IQTCDPRDK GAQTKRDNY TNVPKPSTY IFGLRGRKD 
DKQFIVDLT RPVEE
    CDS sequence
    • >TC012458 RA
      atgcattgt gacggagct atcaggaac atagagaaa 
gaaatactc cgcgaatgt gcttacaaa ggcatccaa 
gtccatgaa aacttcctc aattactac ctcaaactc 
ctctcactc gaccccaca tggggcatc acaggaaaa 
gacgtgcta gacaggagc gacgtccaa aatttcatc 
aaatatgta gtccaccaa ctcgaaaat cagaacacc 
ccgagcatg atcacccta aaaatgcag ttctacttc 
acttgcaac ttccaaatg aagcagaaa atggtgaaa 
aggaacaga actgagctc ttgatgcgg ctgcgaggc 
ttggaacag gagataatc gatgttaaa tacttgact 
gaagaagac gagattaat ttgatgtat aaaaagatc 
gtctattac atcacactg gcttcgggg ttgggaaat 
ccaacattt gacgctgtt tttaaggaa gttagggtc 
gctcttgga agcatcctc gaccggaac gaactgaac 
gacttcgtg gcacaaccc cgttttacc aaaattgat 
aatttggcc gaattcact aaactagtg acgggaatt 
cggctcttc aacaaagac tgcggcaaa ggaggcgag 
ggaatggaa gacttgcca aaactgatc aatgaggca 
cttattgcc acaaatgcc gagcttcaa aacacccta 
atctcaatc atggataaa gtcaattta atgaccacg 
gctctagat tttgtgtgt gggacagaa atggacgaa 
agacacaac tatgttatc gcttatcgc ctcccgaaa 
ggtgtgaca atggccgat gttaactat gttaaagat 
tcgcttatt gcctaccgc caacatgag ctctacgtc 
cggaaaatg atggatgag attgacaga attgaaagt 
acttccgca caaattttt accattctc caacgggcg 
cttattgac ctccaccga gtggtcaag catcggatt 
gcaattcca gtcaatcaa gtctttccc catttcatc 
aagttatcg gacatttgg tgcaacctc caagaccaa 
acaatcctc cttgccaag cacaaccaa atcatgatg 
aatctccag aaatacatc aaagtgttg tcatttccc 
gattccatc gccgaaatg atgatacca ccgggggaa 
tccccgatg accgatacc gagcggttg caaaaaacc 
gccgaccag aaaatccca caggaggac aaccactcc 
atggtctta gacccgttg ctctacacc gacttgaat 
aaattaaaa tatgagtat ttgggtttt tgtgcgtgg 
aagattttc atgacggaa ggggccctc ctacccgga 
aatccaggt ctgggcatc gccaaaacc atgaatatg 
cagttcgtg ttttcgtcg gtggaggcg ggaattgcc 
tttgggaag tgtcccgaa aagtgtgtc atggagaca 
attaagttg gcgaggagg aaacccgaa ctgattaat 
ttgctggcg attaaggat cgattgatt ctcgtgcaa 
aatattaga gatctggtg catgaacca ccgcttttg 
cccttgacg gacgaagtt gaggttcaa acagaaatg 
catcctgtt gaaagtaat attgaccga aattacttc 
tggaatgtg tgggatttg aggaggaaa gcaatagaa 
tatgcgaat ttaactcat tgtcaaact gttagttgt 
caaactaag aagagttat tatagattt aactcgaag 
atacagact tgtgacccc agagataag ggggcgcaa 
acaaagagg gataactac accaatgtt ccaaagcct 
tcaacttat atctttggt ttgagaggt cgcaaagac 
gataaacag tttatcgtt gacttgacc agacctgtt 
gaggagtaa
    mRNA sequence
    • >TC012458 RA
      taaaagtgt tattctagg ttgccaacc attatgttc 
cgcttctat aaaagtgtt attctaggc tgacagtgt 
ttctacctt cagccatgc attgtgacg gagctatca 
ggaacatag agaaagaaa tactccgcg aatgtgctt 
acaaaggca tccaagtcc atgaaaact tcctcaatt 
actacctca aactcctct cactcgacc ccacatggg 
gcatcacag gaaaagacg tgctagaca ggagcgacg 
tccaaaatt tcatcaaat atgtagtcc accaactcg 
aaaatcaga acaccccga gcatgatca ccctaaaaa 
tgcagttct acttcactt gcaacttcc aaatgaagc 
agaaaatgg tgaaaagga acagaactg agctcttga 
tgcggctgc gaggcttgg aacaggaga taatcgatg 
ttaaatact tgactgaag aagacgaga ttaatttga 
tgtataaaa agatcgtct attacatca cactggctt 
cggggttgg gaaatccaa catttgacg ctgttttta 
aggaagtta gggtcgctc ttggaagca tcctcgacc 
ggaacgaac tgaacgact tcgtggcac aaccccgtt 
ttaccaaaa ttgataatt tggccgaat tcactaaac 
tagtgacgg gaattcggc tcttcaaca aagactgcg 
gcaaaggag gcgagggaa tggaagact tgccaaaac 
tgatcaatg aggcactta ttgccacaa atgccgagc 
ttcaaaaca ccctaatct caatcatgg ataaagtca 
atttaatga ccacggctc tagattttg tgtgtggga 
cagaaatgg acgaaagac acaactatg ttatcgctt 
atcgcctcc cgaaaggtg tgacaatgg ccgatgtta 
actatgtta aagattcgc ttattgcct accgccaac 
atgagctct acgtccgga aaatgatgg atgagattg 
acagaattg aaagtactt ccgcacaaa tttttacca 
ttctccaac gggcgctta ttgacctcc accgagtgg 
tcaagcatc ggattgcaa ttccagtca atcaagtct 
ttccccatt tcatcaagt tatcggaca tttggtgca 
acctccaag accaaacaa tcctccttg ccaagcaca 
accaaatca tgatgaatc tccagaaat acatcaaag 
tgttgtcat ttcccgatt ccatcgccg aaatgatga 
taccaccgg gggaatccc cgatgaccg ataccgagc 
ggttgcaaa aaaccgccg accagaaaa tcccacagg 
aggacaacc actccatgg tcttagacc cgttgctct 
acaccgact tgaataaat taaaatatg agtatttgg 
gtttttgtg cgtggaaga ttttcatga cggaagggg 
ccctcctac ccggaaatc caggtctgg gcatcgcca 
aaaccatga atatgcagt tcgtgtttt cgtcggtgg 
aggcgggaa ttgcctttg ggaagtgtc ccgaaaagt 
gtgtcatgg agacaatta agttggcga ggaggaaac 
ccgaactga ttaatttgc tggcgatta aggatcgat 
tgattctcg tgcaaaata ttagagatc tggtgcatg 
aaccaccgc ttttgccct tgacggacg aagttgagg 
ttcaaacag aaatgcatc ctgttgaaa gtaatattg 
accgaaatt acttctgga atgtgtggg atttgagga 
ggaaagcaa tagaatatg cgaatttaa ctcattgtc 
aaactgtta gttgtcaaa ctaagaaga gttattata 
gatttaact cgaagatac agacttgtg accccagag 
ataaggggg cgcaaacaa agagggata actacacca 
atgttccaa agccttcaa cttatatct ttggtttga 
gaggtcgca aagacgata aacagttta tcgttgact 
tgaccagac ctgttgagg agtaaataa aatacttta 
gcagtaaaa atgcgtttt ttttttttc aattaatac 
cgtatttca gaaaaatta tcatcatca agtatcatc 
gaagtatac caggtgagg caattttat cgcgcatct 
aagaaaatc gtgattttt ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY