Status
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Gene information
General information
TC012458 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 8360358..8366403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012458
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01981

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012458 RA
      MHCDGAIRN IEKEILREC AYKGIQVHE NFLNYYLKL 
      LSLDPTWGI TGKDVLDRS DVQNFIKYV VHQLENQNT 
      PSMITLKMQ FYFTCNFQM KQKMVKRNR TELLMRLRG 
      LEQEIIDVK YLTEEDEIN LMYKKIVYY ITLASGLGN 
      PTFDAVFKE VRVALGSIL DRNELNDFV AQPRFTKID 
      NLAEFTKLV TGIRLFNKD CGKGGEGME DLPKLINEA 
      LIATNAELQ NTLISIMDK VNLMTTALD FVCGTEMDE 
      RHNYVIAYR LPKGVTMAD VNYVKDSLI AYRQHELYV 
      RKMMDEIDR IESTSAQIF TILQRALID LHRVVKHRI 
      AIPVNQVFP HFIKLSDIW CNLQDQTIL LAKHNQIMM 
      NLQKYIKVL SFPDSIAEM MIPPGESPM TDTERLQKT 
      ADQKIPQED NHSMVLDPL LYTDLNKLK YEYLGFCAW 
      KIFMTEGAL LPGNPGLGI AKTMNMQFV FSSVEAGIA 
      FGKCPEKCV METIKLARR KPELINLLA IKDRLILVQ 
      NIRDLVHEP PLLPLTDEV EVQTEMHPV ESNIDRNYF 
      WNVWDLRRK AIEYANLTH CQTVSCQTK KSYYRFNSK 
      IQTCDPRDK GAQTKRDNY TNVPKPSTY IFGLRGRKD 
      DKQFIVDLT RPVEE
    CDS sequence
    • >TC012458 RA
      atgcattgt gacggagct atcaggaac atagagaaa 
      gaaatactc cgcgaatgt gcttacaaa ggcatccaa 
      gtccatgaa aacttcctc aattactac ctcaaactc 
      ctctcactc gaccccaca tggggcatc acaggaaaa 
      gacgtgcta gacaggagc gacgtccaa aatttcatc 
      aaatatgta gtccaccaa ctcgaaaat cagaacacc 
      ccgagcatg atcacccta aaaatgcag ttctacttc 
      acttgcaac ttccaaatg aagcagaaa atggtgaaa 
      aggaacaga actgagctc ttgatgcgg ctgcgaggc 
      ttggaacag gagataatc gatgttaaa tacttgact 
      gaagaagac gagattaat ttgatgtat aaaaagatc 
      gtctattac atcacactg gcttcgggg ttgggaaat 
      ccaacattt gacgctgtt tttaaggaa gttagggtc 
      gctcttgga agcatcctc gaccggaac gaactgaac 
      gacttcgtg gcacaaccc cgttttacc aaaattgat 
      aatttggcc gaattcact aaactagtg acgggaatt 
      cggctcttc aacaaagac tgcggcaaa ggaggcgag 
      ggaatggaa gacttgcca aaactgatc aatgaggca 
      cttattgcc acaaatgcc gagcttcaa aacacccta 
      atctcaatc atggataaa gtcaattta atgaccacg 
      gctctagat tttgtgtgt gggacagaa atggacgaa 
      agacacaac tatgttatc gcttatcgc ctcccgaaa 
      ggtgtgaca atggccgat gttaactat gttaaagat 
      tcgcttatt gcctaccgc caacatgag ctctacgtc 
      cggaaaatg atggatgag attgacaga attgaaagt 
      acttccgca caaattttt accattctc caacgggcg 
      cttattgac ctccaccga gtggtcaag catcggatt 
      gcaattcca gtcaatcaa gtctttccc catttcatc 
      aagttatcg gacatttgg tgcaacctc caagaccaa 
      acaatcctc cttgccaag cacaaccaa atcatgatg 
      aatctccag aaatacatc aaagtgttg tcatttccc 
      gattccatc gccgaaatg atgatacca ccgggggaa 
      tccccgatg accgatacc gagcggttg caaaaaacc 
      gccgaccag aaaatccca caggaggac aaccactcc 
      atggtctta gacccgttg ctctacacc gacttgaat 
      aaattaaaa tatgagtat ttgggtttt tgtgcgtgg 
      aagattttc atgacggaa ggggccctc ctacccgga 
      aatccaggt ctgggcatc gccaaaacc atgaatatg 
      cagttcgtg ttttcgtcg gtggaggcg ggaattgcc 
      tttgggaag tgtcccgaa aagtgtgtc atggagaca 
      attaagttg gcgaggagg aaacccgaa ctgattaat 
      ttgctggcg attaaggat cgattgatt ctcgtgcaa 
      aatattaga gatctggtg catgaacca ccgcttttg 
      cccttgacg gacgaagtt gaggttcaa acagaaatg 
      catcctgtt gaaagtaat attgaccga aattacttc 
      tggaatgtg tgggatttg aggaggaaa gcaatagaa 
      tatgcgaat ttaactcat tgtcaaact gttagttgt 
      caaactaag aagagttat tatagattt aactcgaag 
      atacagact tgtgacccc agagataag ggggcgcaa 
      acaaagagg gataactac accaatgtt ccaaagcct 
      tcaacttat atctttggt ttgagaggt cgcaaagac 
      gataaacag tttatcgtt gacttgacc agacctgtt 
      gaggagtaa
    mRNA sequence
    • >TC012458 RA
      taaaagtgt tattctagg ttgccaacc attatgttc 
      cgcttctat aaaagtgtt attctaggc tgacagtgt 
      ttctacctt cagccatgc attgtgacg gagctatca 
      ggaacatag agaaagaaa tactccgcg aatgtgctt 
      acaaaggca tccaagtcc atgaaaact tcctcaatt 
      actacctca aactcctct cactcgacc ccacatggg 
      gcatcacag gaaaagacg tgctagaca ggagcgacg 
      tccaaaatt tcatcaaat atgtagtcc accaactcg 
      aaaatcaga acaccccga gcatgatca ccctaaaaa 
      tgcagttct acttcactt gcaacttcc aaatgaagc 
      agaaaatgg tgaaaagga acagaactg agctcttga 
      tgcggctgc gaggcttgg aacaggaga taatcgatg 
      ttaaatact tgactgaag aagacgaga ttaatttga 
      tgtataaaa agatcgtct attacatca cactggctt 
      cggggttgg gaaatccaa catttgacg ctgttttta 
      aggaagtta gggtcgctc ttggaagca tcctcgacc 
      ggaacgaac tgaacgact tcgtggcac aaccccgtt 
      ttaccaaaa ttgataatt tggccgaat tcactaaac 
      tagtgacgg gaattcggc tcttcaaca aagactgcg 
      gcaaaggag gcgagggaa tggaagact tgccaaaac 
      tgatcaatg aggcactta ttgccacaa atgccgagc 
      ttcaaaaca ccctaatct caatcatgg ataaagtca 
      atttaatga ccacggctc tagattttg tgtgtggga 
      cagaaatgg acgaaagac acaactatg ttatcgctt 
      atcgcctcc cgaaaggtg tgacaatgg ccgatgtta 
      actatgtta aagattcgc ttattgcct accgccaac 
      atgagctct acgtccgga aaatgatgg atgagattg 
      acagaattg aaagtactt ccgcacaaa tttttacca 
      ttctccaac gggcgctta ttgacctcc accgagtgg 
      tcaagcatc ggattgcaa ttccagtca atcaagtct 
      ttccccatt tcatcaagt tatcggaca tttggtgca 
      acctccaag accaaacaa tcctccttg ccaagcaca 
      accaaatca tgatgaatc tccagaaat acatcaaag 
      tgttgtcat ttcccgatt ccatcgccg aaatgatga 
      taccaccgg gggaatccc cgatgaccg ataccgagc 
      ggttgcaaa aaaccgccg accagaaaa tcccacagg 
      aggacaacc actccatgg tcttagacc cgttgctct 
      acaccgact tgaataaat taaaatatg agtatttgg 
      gtttttgtg cgtggaaga ttttcatga cggaagggg 
      ccctcctac ccggaaatc caggtctgg gcatcgcca 
      aaaccatga atatgcagt tcgtgtttt cgtcggtgg 
      aggcgggaa ttgcctttg ggaagtgtc ccgaaaagt 
      gtgtcatgg agacaatta agttggcga ggaggaaac 
      ccgaactga ttaatttgc tggcgatta aggatcgat 
      tgattctcg tgcaaaata ttagagatc tggtgcatg 
      aaccaccgc ttttgccct tgacggacg aagttgagg 
      ttcaaacag aaatgcatc ctgttgaaa gtaatattg 
      accgaaatt acttctgga atgtgtggg atttgagga 
      ggaaagcaa tagaatatg cgaatttaa ctcattgtc 
      aaactgtta gttgtcaaa ctaagaaga gttattata 
      gatttaact cgaagatac agacttgtg accccagag 
      ataaggggg cgcaaacaa agagggata actacacca 
      atgttccaa agccttcaa cttatatct ttggtttga 
      gaggtcgca aagacgata aacagttta tcgttgact 
      tgaccagac ctgttgagg agtaaataa aatacttta 
      gcagtaaaa atgcgtttt ttttttttc aattaatac 
      cgtatttca gaaaaatta tcatcatca agtatcatc 
      gaagtatac caggtgagg caattttat cgcgcatct 
      aagaaaatc gtgattttt ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY