Status
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Gene information
General information
TC012387 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6022515..6034171
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012387
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01975

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Adult morphology
    odoriferous glands darker - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012387 RA
      MRRGMPNAD NKFRHDASK TDKHTLGNL DLLNLRFSS 
      PPDKFKNKL EKEAASGDN RLPEKTKLR HSFQRLNLG 
      ESKLCKDYA QTKTTRKPT TARVDPTID DFLLPPGKR 
      NSREFSSSR STFQLSSAG DAGNFPFKV TPSGNLKVN 
      SQQVVFESK KLSVLVANP ERTQIKIYS DESSDKIPK 
      KMAYGSGEF TRNSAMDAI LSQVTGGEI SGLGSIIGD 
      TPARSMNKV SSEYESKQT SEILDPELN LCRLGYTGS 
      EPIDWNKVT LPEKKDLYL ELCKRITNK RNADCKVHI 
      GREEFSCHL VVLQCYSEL FDGYIAIKK VEIPAEKCS 
      AASFAFIYD WMVSEDQSY KYLTRENVL DIFNSAKYL 
      KIKDLVEQC WAFVDNCEV FNEDTAFLL YVNAKQKRL 
      EEVHELMVP RIQDFFLIL VSSQDWLEL GVEDVKNLL 
      SSNYIRINC EMEVFMSAV RWLKYDWAN RDKYKYEVL 
      KCVRFGNIA AWQLVDIKR NPENPEFME LAKDPAICK 
      LIDDGLAFV IIKHWYDHD NDDFQHWNS VLGLQEPPA 
      RNWSGLDKT YFTYREFLI YLDQYRRNQ LIEKSKPRM 
      NHEKKLGAP SGTVMSPEE PVPSMEDFL SKKKALRMQ 
      RKPVIVQRV EVRQGVHFD NFEDDEVRN VQRMFTPSQ 
      NSLALTSLP QPKKLLVES TLFQAERET ILVFGGINP 
      YANYGEPRN TGNNIFRYL PDSNMWEHV GTMPEPRNH 
      HGVGYLKGR IYLAGGTDP RPDDLRGKS RVVDTVWSF 
      DPTTRAWFS ETSLGMKRR NFGLVVLQK NMYVIGGCN 
      DKFESLNSV EKFDPREGV WKFMAPMHY ARAGLACAK 
      YRNFIWAAG GTADLKRNL MLDVVESYD VRSNQWTKI 
      KKLISPRCF GCLFVMADN LYLIGGTGQ KQKEFKSTT 
      SVGLVDVWD TSNMSWRTI MGMTIPRHG HAVAYVGTQ 
      IFIMGGVTT IYMRCLSNI ECFCWKRGA WIRGIADLP 
      VTLSGHAAI TLPPAMLIS NE
    CDS sequence
    • >TC012387 RA
      atgcgtaga ggtatgccc aatgctgat aataaattt 
      agacacgac gcatcgaaa actgacaag cacacttta 
      ggcaactta gatttgtta aatttacgt tttagttca 
      cctcctgat aagtttaag aacaagcta gaaaaggaa 
      gcggcttcc ggagacaat cgactccca gagaaaacc 
      aaattaaga cactcgttc caaagactc aacttgggg 
      gaatccaag ttgtgcaaa gattatgct cagactaaa 
      acaactcgt aaaccgacg acagcgaga gtagaccca 
      acaatcgac gatttttta ctgccaccg ggaaaacga 
      aactcaaga gaattcagc tcgagcaga tcaacgttt 
      cagttaagt agcgcaggt gacgccgga aatttcccg 
      tttaaggtt acccccagt gggaattta aaagtcaat 
      tcgcagcag gtcgttttc gagtcgaaa aagctcagt 
      gttttagtg gcaaacccg gaacgcacc caaatcaag 
      atatactcg gacgagtcc agcgataaa ataccgaaa 
      aaaatggcc tacggttcc ggggaattc acccggaat 
      tcggccatg gacgccatc ttgagccaa gtgactggg 
      ggcgagatt tccggtctg ggctccatt atcggggac 
      acccccgcc cggagcatg aataaggta tcatccgag 
      tacgaaagc aaacaaact tcggaaatc ttggacccg 
      gagttgaac ctctgccgg ctggggtac accggaagc 
      gagccgatc gactggaac aaggtcacc cttcccgag 
      aagaaagac ctgtatttg gaattgtgt aaacgtatc 
      accaataaa agaaatgcg gactgtaag gtgcacatt 
      gggcgagaa gaatttagc tgtcacctc gttgtcttg 
      cagtgttac tctgagctt ttcgacggc tacattgca 
      ataaagaag gtggaaatt ccggctgaa aaatgcagt 
      gcggcttcg ttcgctttc atttacgac tggatggtg 
      agcgaggac cagtcttat aaatacctg actcgggag 
      aatgtgctc gatattttc aattcagcg aaatatttg 
      aaaattaaa gatttggtc gagcagtgc tgggcgttt 
      gtcgacaat tgcgaggtt ttcaacgaa gacacggct 
      tttcttctg tacgtgaac gcgaaacag aagcgcctt 
      gaagaagtc cacgagttg atggtgccg cgtatccag 
      gacttcttc ctgattttg gtcagttcg caggactgg 
      ctcgaactg ggagtggag gacgttaag aatttgctc 
      agctcgaac tatatcagg atcaactgc gagatggag 
      gtgttcatg tccgcagtc aggtggctc aagtacgac 
      tgggccaac agggataag tacaagtat gaagtgctc 
      aagtgcgtc agattcgga aacatagcc gcttggcaa 
      cttgttgat atcaagcgc aatcccgaa aatcccgaa 
      tttatggag ttagccaaa gacccagcc atttgcaag 
      ctcatcgat gacgggctg gcgtttgtg atcatcaag 
      cactggtac gaccacgac aacgacgac ttccagcac 
      tggaactcg gtcctgggg ctgcaggag ccgccggcc 
      aggaactgg agcgggctg gacaagacg tacttcacg 
      taccgcgag ttcttgatc tacttggac cagtaccgc 
      cggaatcag ttgattgag aagagcaag cctcgcatg 
      aaccacgag aagaaactg ggggcgcct tccggcacg 
      gtgatgtcg ccggaggag ccggtgccc agcatggaa 
      gattttttg tcgaagaag aaagcgtta cgaatgcaa 
      cgcaagccg gtgattgtc cagagggtg gaagtcagg 
      caaggagtg cattttgac aatttcgaa gacgatgaa 
      gttaggaat gtgcaaagg atgtttacg ccgtctcag 
      aactcgcta gctttgacg tcccttccg cagcctaaa 
      aaactgctc gtagagtcg acactgttc caagccgaa 
      agggaaacg attttagtt tttggcggg attaatccg 
      tacgcaaat tatggtgag ccgagaaat acgggaaat 
      aatattttt cggtactta ccggactcc aatatgtgg 
      gaacatgtc ggtactatg cccgaaccg aggaaccat 
      cacggggtc ggctacctc aaggggagg atttattta 
      gcaggcggt accgacccc agacccgac gacttgcgc 
      ggcaaatct cgagtagtt gacacagtt tggagcttc 
      gaccccaca acccgagcc tggttcagc gaaaccagt 
      cttggtatg aaacgtcga aattttgga ctggtggta 
      ctccaaaaa aacatgtat gtgattgga ggttgcaac 
      gataagttc gaatcgttg aattctgtc gaaaaattc 
      gaccccaga gaaggcgtt tggaagttt atggcaccc 
      atgcattac gccagggcc ggacttgcc tgtgccaag 
      taccggaat ttcatttgg gcggccggt ggtaccgcc 
      gacctaaaa cgaaacctt atgctagat gttgtggaa 
      agttacgac gtgaggagt aaccaatgg accaaaatt 
      aagaaactc atttcgcca cgatgtttc ggttgtttg 
      tttgtcatg gcagataat ctgtatttg atcggtggt 
      accggtcag aaacagaaa gagtttaaa tcaacgacc 
      agtgttggg cttgtggat gtttgggat acttcgaat 
      atgtcgtgg cggaccatc atgggcatg actatacca 
      cgacatggt catgctgtt gcgtatgtt ggtacccag 
      atttttatc atgggtggt gtcacaacc atttatatg 
      agatgtcta tccaacatt gagtgtttt tgttggaag 
      cgaggtgct tggataagg ggcattgcc gatttaccg 
      gtgacctta tcaggtcac gctgctatc actcttcct 
      cccgccatg ctaatttca aacgaataa
    mRNA sequence
    • >TC012387 RA
      gttgcaatg tgtcaaact tgtcatttt cagtacgaa 
      atgtctgtg ttagtgagt ggagccaaa attgtagta 
      atgattttg aacggtaaa aaccaaagt aaatttaaa 
      accacagtc ccaaccaaa atacgatag aatcctgta 
      ggaaatttg tagtatttc gcatataat gcgtagagg 
      tatgcccaa tgctgataa taaatttag acacgacgc 
      atcgaaaac tgacaagca cactttagg caacttaga 
      tttgttaaa tttacgttt tagttcacc tcctgataa 
      gtttaagaa caagctaga aaaggaagc ggcttccgg 
      agacaatcg actcccaga gaaaaccaa attaagaca 
      ctcgttcca aagactcaa cttggggga atccaagtt 
      gtgcaaaga ttatgctca gactaaaac aactcgtaa 
      accgacgac agcgagagt agacccaac aatcgacga 
      ttttttact gccaccggg aaaacgaaa ctcaagaga 
      attcagctc gagcagatc aacgtttca gttaagtag 
      cgcaggtga cgccggaaa tttcccgtt taaggttac 
      ccccagtgg gaatttaaa agtcaattc gcagcaggt 
      cgttttcga gtcgaaaaa gctcagtgt tttagtggc 
      aaacccgga acgcaccca aatcaagat atactcgga 
      cgagtccag cgataaaat accgaaaaa aatggccta 
      cggttccgg ggaattcac ccggaattc ggccatgga 
      cgccatctt gagccaagt gactggggg cgagatttc 
      cggtctggg ctccattat cggggacac ccccgcccg 
      gagcatgaa taaggtatc atccgagta cgaaagcaa 
      acaaacttc ggaaatctt ggacccgga gttgaacct 
      ctgccggct ggggtacac cggaagcga gccgatcga 
      ctggaacaa ggtcaccct tcccgagaa gaaagacct 
      gtatttgga attgtgtaa acgtatcac caataaaag 
      aaatgcgga ctgtaaggt gcacattgg gcgagaaga 
      atttagctg tcacctcgt tgtcttgca gtgttactc 
      tgagctttt cgacggcta cattgcaat aaagaaggt 
      ggaaattcc ggctgaaaa atgcagtgc ggcttcgtt 
      cgctttcat ttacgactg gatggtgag cgaggacca 
      gtcttataa atacctgac tcgggagaa tgtgctcga 
      tattttcaa ttcagcgaa atatttgaa aattaaaga 
      tttggtcga gcagtgctg ggcgtttgt cgacaattg 
      cgaggtttt caacgaaga cacggcttt tcttctgta 
      cgtgaacgc gaaacagaa gcgccttga agaagtcca 
      cgagttgat ggtgccgcg tatccagga cttcttcct 
      gattttggt cagttcgca ggactggct cgaactggg 
      agtggagga cgttaagaa tttgctcag ctcgaacta 
      tatcaggat caactgcga gatggaggt gttcatgtc 
      cgcagtcag gtggctcaa gtacgactg ggccaacag 
      ggataagta caagtatga agtgctcaa gtgcgtcag 
      attcggaaa catagccgc ttggcaact tgttgatat 
      caagcgcaa tcccgaaaa tcccgaatt tatggagtt 
      agccaaaga cccagccat ttgcaagct catcgatga 
      cgggctggc gtttgtgat catcaagca ctggtacga 
      ccacgacaa cgacgactt ccagcactg gaactcggt 
      cctggggct gcaggagcc gccggccag gaactggag 
      cgggctgga caagacgta cttcacgta ccgcgagtt 
      cttgatcta cttggacca gtaccgccg gaatcagtt 
      gattgagaa gagcaagcc tcgcatgaa ccacgagaa 
      gaaactggg ggcgccttc cggcacggt gatgtcgcc 
      ggaggagcc ggtgcccag catggaaga ttttttgtc 
      gaagaagaa agcgttacg aatgcaacg caagccggt 
      gattgtcca gagggtgga agtcaggca aggagtgca 
      ttttgacaa tttcgaaga cgatgaagt taggaatgt 
      gcaaaggat gtttacgcc gtctcagaa ctcgctagc 
      tttgacgtc ccttccgca gcctaaaaa actgctcgt 
      agagtcgac actgttcca agccgaaag ggaaacgat 
      tttagtttt tggcgggat taatccgta cgcaaatta 
      tggtgagcc gagaaatac gggaaataa tatttttcg 
      gtacttacc ggactccaa tatgtggga acatgtcgg 
      tactatgcc cgaaccgag gaaccatca cggggtcgg 
      ctacctcaa ggggaggat ttatttagc aggcggtac 
      cgaccccag acccgacga cttgcgcgg caaatctcg 
      agtagttga cacagtttg gagcttcga ccccacaac 
      ccgagcctg gttcagcga aaccagtct tggtatgaa 
      acgtcgaaa ttttggact ggtggtact ccaaaaaaa 
      catgtatgt gattggagg ttgcaacga taagttcga 
      atcgttgaa ttctgtcga aaaattcga ccccagaga 
      aggcgtttg gaagtttat ggcacccat gcattacgc 
      cagggccgg acttgcctg tgccaagta ccggaattt 
      catttgggc ggccggtgg taccgccga cctaaaacg 
      aaaccttat gctagatgt tgtggaaag ttacgacgt 
      gaggagtaa ccaatggac caaaattaa gaaactcat 
      ttcgccacg atgtttcgg ttgtttgtt tgtcatggc 
      agataatct gtatttgat cggtggtac cggtcagaa 
      acagaaaga gtttaaatc aacgaccag tgttgggct 
      tgtggatgt ttgggatac ttcgaatat gtcgtggcg 
      gaccatcat gggcatgac tataccacg acatggtca 
      tgctgttgc gtatgttgg tacccagat ttttatcat 
      gggtggtgt cacaaccat ttatatgag atgtctatc 
      caacattga gtgtttttg ttggaagcg aggtgcttg 
      gataagggg cattgccga tttaccggt gaccttatc 
      aggtcacgc tgctatcac tcttcctcc cgccatgct 
      aatttcaaa cgaataagt gataaaaac aggaaacaa 
      tatttgatg aattaaaga ttcctgtcg tgggcgttg 
      aatgagtgc ggcatctga ttgggagat ggcgccgcg 
      ctacttgaa gtactttta caccataac catagttta 
      gctttagaa attataaat ttagcgttt ttttatact 
      ctaatgttg tatttaaaa atgttatac acgaccgta 
      catttgctg cttttttga tacatgaaa taaagattt 
      ttaccttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY