Status
Please wait ...
Gene information
General information
TC034313
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012382
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5905996..5907584
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034313
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01974

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034313 RA
      MTEVVKVTD ENNNENKLL IDSFDSEPG YYCQVIRSM 
      LTPGHETEL VYWLKSFGL IPNTQICNN ANINEDCNK 
      IMEWTPVKT IDYYQWKCR NCSGKKSIR ENSVFQDVK 
      CNFKNSIRI LLGWCKGYD AELMANILG VKSRTVKVL 
      YNAVSATAR KHLKSQQNK KVGGPGIIV LVDTYPEGC 
      TNSSRPNQA NRPILCIAE IKDIPPNYW FEALNRYHR 
      NNVQQIMDV KEHILTVIQ DVVVPGSIL VSRQDSTLC 
      SFFDLQKLS HIYPTIVSV EGLARHNSA NQEILANLE 
      IIWKLALNV CEEAQFFNY EQVPNFFIS QMWHSRFGN 
      ESFETLLNR LADLQ
    CDS sequence
    • >TC034313 RA
      atgaccgag gtcgtaaaa gtaaccgac gaaaacaac 
      aacgagaac aagttactg atcgactcc ttcgatagc 
      gagcccggc tactactgc caagtgatc cgttcgatg 
      ctgactccc ggccacgag accgaactc gtctactgg 
      ctcaaaagc ttcggcttg atccccaac acccaaatt 
      tgcaacaac gcgaacata aatgaagat tgcaacaag 
      ataatggag tggacgccg gtcaaaacc atcgactac 
      taccagtgg aagtgccgg aattgcagc gggaagaaa 
      agcatcagg gagaactcg gtgtttcaa gatgttaag 
      tgtaatttc aagaactct atcaggatt ttgctgggg 
      tggtgcaaa gggtacgac gctgaattg atggccaac 
      atcttaggt gttaaatca cgtactgtc aaggttctc 
      tacaacgcg gtttcggca acagcacga aaacaccta 
      aagtcgcaa caaaataaa aaggttggg ggccctgga 
      atcattgtt ttagttgat acgtatccc gaaggttgc 
      acaaacagc tcaaggcca aaccaagcc aatagaccc 
      atactgtgc attgctgaa ataaaggac attcccccc 
      aattattgg ttcgaggcc ttaaaccgg taccatcgc 
      aacaacgtg cagcaaata atggacgtg aaagagcac 
      attttgacg gtgattcaa gacgtcgtc gtccctggt 
      tccattctg gtctcgcga caagactcc actctgtgt 
      tcgtttttt gatttgcaa aaattgtcc cacatttac 
      cccaccata gtgagcgtt gaaggcctc gcacgtcac 
      aattcagcc aatcaggaa atcctagcc aacttggaa 
      attatctgg aagttggcg ttgaatgtg tgcgaggaa 
      gcccagttt tttaattat gaacaagtg ccgaatttt 
      ttcatcagc caaatgtgg cattcgcga tttggtaac 
      gaatcgttt gaaacgctc ctgaatcgt ctcgccgac 
      ttgcaataa
    mRNA sequence
    • >TC034313 RA
      cagtcaaaa aatgtcaat cgatccgcc attcttggc 
      cttttccaa tttatttat aaacaaagg gaatattat 
      gtgaactag ttaattatt tctcctcca aaattggct 
      ggtttatga tgaccgagg tcgtaaaag taaccgacg 
      aaaacaaca acgagaaca agttactga tcgactcct 
      tcgatagcg agcccggct actactgcc aagtgatcc 
      gttcgatgc tgactcccg gccacgaga ccgaactcg 
      tctactggc tcaaaagct tcggcttga tccccaaca 
      cccaaattt gcaacaacg cgaacataa atgaagatt 
      gcaacaaga taatggagt ggacgccgg tcaaaacca 
      tcgactact accagtgga agtgccgga attgcagcg 
      ggaagaaaa gcatcaggg agaactcgg tgtttcaag 
      atgttaagt gtaatttca agaactcta tcaggattt 
      tgctggggt ggtgcaaag ggtacgacg ctgaattga 
      tggccaaca tcttaggtg ttaaatcac gtactgtca 
      aggttctct acaacgcgg tttcggcaa cagcacgaa 
      aacacctaa agtcgcaac aaaataaaa aggttgggg 
      gccctggaa tcattgttt tagttgata cgtatcccg 
      aaggttgca caaacagct caaggccaa accaagcca 
      atagaccca tactgtgca ttgctgaaa taaaggaca 
      ttcccccca attattggt tcgaggcct taaaccggt 
      accatcgca acaacgtgc agcaaataa tggacgtga 
      aagagcaca ttttgacgg tgattcaag acgtcgtcg 
      tccctggtt ccattctgg tctcgcgac aagactcca 
      ctctgtgtt cgttttttg atttgcaaa aattgtccc 
      acatttacc ccaccatag tgagcgttg aaggcctcg 
      cacgtcaca attcagcca atcaggaaa tcctagcca 
      acttggaaa ttatctgga agttggcgt tgaatgtgt 
      gcgaggaag cccagtttt ttaattatg aacaagtgc 
      cgaattttt tcatcagcc aaatgtggc attcgcgat 
      ttggtaacg aatcgtttg aaacgctcc tgaatcgtc 
      tcgccgact tgcaataac ggatatttt tcgaagcaa 
      taattgtgt ttatgttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY