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Gene information
General information
TC012376 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 2949388..2966047
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012376
    iBeetle screen iB_01973
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection



    Pupal morphology
    head slightly shape irregular - Number of animals showing the phenotype: 3

    wings shortened - Number of animals showing the phenotype: 3

    legs potentially shortened - Number of animals showing the phenotype: 3
    legs orientation irregular - Number of animals showing the phenotype: 3

    wings orientation irregular - Number of animals showing the phenotype: 3
    head slightly shape irregular - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    wings shortened - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    wings orientation irregular - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    legs potentially shortened - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    prothoracic leg shortened - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    legs orientation irregular - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012376 RA
      MDGVGGRRR IAAKSSAED QALDQIAKE AEARLWAKR 
      QARAEAREI RMRELERQQ KEQEENADR QFDMLAEPM 
      ARTARTVTA GSRYLSSRR SSEDSLEDG GSLRDLRNE 
      LKEYEEKFR KAMIANAQL DNDKTSLTY QLELLKDRL 
      EELEEEHSQ LKREHREKC RDFDQLKRL CAKLKDDLA 
      VTRAELEER DQLISEKGL VIVGEDSIN EEEQRNGDV 
      SLSSIGTPK KALVSVENA QLLQSAGEG SLDVRLAKL 
      MKEKGELQD QISHLKLEL EEERSKRKK SSAGGTLNG 
      PSSDPDLDE IDIQREASK QLGDYKFRA QKAEQDVAT 
      LQATVARLE SQVIRYKAA AEVSEKAEE ALKAEKRKL 
      QREAREAQS RAEELETSN THLLKRLDK LKNAKSALL 
      KEL
    CDS sequence
    • >TC012376 RA
      atggacggc gttggcggc cgacgacgt atcgcagcc 
      aagtcgtct gcagaggac caagcgctt gaccaaatc 
      gccaaagag gcggaagca cgtttgtgg gccaaaaga 
      caggcccga gctgaagct agagagata cgtatgcgc 
      gaactggag aggcaacag aaagaacaa gaggaaaat 
      gccgaccga caattcgat atgttggca gaaccgatg 
      gccaggact gcgaggacg gttactgca gggagcagg 
      tatctctcc agcaggagg agctccgag gactcgctg 
      gaggacggg ggcagtctc agggatttg cggaacgaa 
      ctaaaagag tatgaggag aagtttcgt aaggcgatg 
      atcgccaac gcccaactc gataacgac aaaacgtct 
      ctcacatat caactggaa ctgcttaag gataggtta 
      gaagaacta gaagaggag cattcgcag ttgaagcga 
      gagcaccgg gaaaagtgc cgcgacttc gaccaatta 
      aaacggttg tgcgcaaaa ttaaaagat gatttggct 
      gtgactcgg gctgaactc gaagaacgc gatcagttg 
      ataagcgag aagggttta gtcatcgtc ggcgaggac 
      tcgatcaac gaagaggaa caaaggaac ggcgatgtc 
      agtcttagt agtataggt acaccgaaa aaggcttta 
      gttagtgta gaaaatgcc cagctgttg caaagcgcg 
      ggcgaaggc agtctggac gtgcgatta gcgaaattg 
      atgaaagaa aaaggcgaa ctgcaggat caaattagc 
      catttaaaa ttagaatta gaagaggag cgaagcaag 
      cggaaaaaa tcgagtgcg ggcggcacg ttgaacggg 
      ccgtcgtcg gaccccgac ttggacgaa atagacatt 
      caaagagag gcgagtaaa cagctcggg gattacaaa 
      ttccgggcg caaaaggcc gagcaagac gtcgccacc 
      ttgcaggcg accgttgct cggctcgag agccaagtc 
      attaggtac aaggctgcg gccgaagtc tcggagaag 
      gccgaggag gccctaaag gccgaaaaa cgcaagctg 
      caaagagag gcacgggag gcgcagagt cgggccgag 
      gaattggaa acatcaaac acacactta ctgaaacgt 
      ttagataaa ttgaagaat gcgaaatca gcgttactg 
      aaagaactt tga
    mRNA sequence
    • >TC012376 RA
      cgaaaaaga aatgtcgtc acaaattta cacatgtgt 
      agaattttg catataact tgcaatatt tcgtaagtt 
      gccctgtga taagataaa atagtgtct cgtccttgt 
      caagaaatt atcatgtgt tgtgttttc ttttgatgt 
      gtgtgtgat aagaattcg taaatctct ttgtaatcg 
      ttacacccc tcaacgtct cgagaggtc gatcgaagt 
      aattgattt tgtctggat ttgcgttac gagccgata 
      atgattatg aaacaggag gaaattaga tgaaccgta 
      agaattgcg agtctggtg tcggtgttg tcctctccg 
      gtaactcct cgccatgga cggcgttgg cggccgacg 
      acgtatcgc agccaagtc gtctgcaga ggaccaagc 
      gcttgacca aatcgccaa agaggcgga agcacgttt 
      gtgggccaa aagacaggc ccgagctga agctagaga 
      gatacgtat gcgcgaact ggagaggca acagaaaga 
      acaagagga aaatgccga ccgacaatt cgatatgtt 
      ggcagaacc gatggccag gactgcgag gacggttac 
      tgcagggag caggtatct ctccagcag gaggagctc 
      cgaggactc gctggagga cgggggcag tctcaggga 
      tttgcggaa cgaactaaa agagtatga ggagaagtt 
      tcgtaaggc gatgatcgc caacgccca actcgataa 
      cgacaaaac gtctctcac atatcaact ggaactgct 
      taaggatag gttagaaga actagaaga ggagcattc 
      gcagttgaa gcgagagca ccgggaaaa gtgccgcga 
      cttcgacca attaaaacg gttgtgcgc aaaattaaa 
      agatgattt ggctgtgac tcgggctga actcgaaga 
      acgcgatca gttgataag cgagaaggg tttagtcat 
      cgtcggcga ggactcgat caacgaaga ggaacaaag 
      gaacggcga tgtcagtct tagtagtat aggtacacc 
      gaaaaaggc tttagttag tgtagaaaa tgcccagct 
      gttgcaaag cgcgggcga aggcagtct ggacgtgcg 
      attagcgaa attgatgaa agaaaaagg cgaactgca 
      ggatcaaat tagccattt aaaattaga attagaaga 
      ggagcgaag caagcggaa aaaatcgag tgcgggcgg 
      cacgttgaa cgggccgtc gtcggaccc cgacttgga 
      cgaaataga cattcaaag agaggcgag taaacagct 
      cggggatta caaattccg ggcgcaaaa ggccgagca 
      agacgtcgc caccttgca ggcgaccgt tgctcggct 
      cgagagcca agtcattag gtacaaggc tgcggccga 
      agtctcgga gaaggccga ggaggccct aaaggccga 
      aaaacgcaa gctgcaaag agaggcacg ggaggcgca 
      gagtcgggc cgaggaatt ggaaacatc aaacacaca 
      cttactgaa acgtttaga taaattgaa gaatgcgaa 
      atcagcgtt actgaaaga actttgaca atcatcgag 
      tagttagtc ggatgtcgg aaggaacgt gcagtgagt 
      tgtgaaacg gaaccagcc taatagtca taatttcat 
      gaaaaatta attttaatt taactcttt taacaaatt 
      ttaacacat gaattcgtc attttaaag tgttgttta 
      tttatttga gttttaaac cttgtgtaa atctgtttt 
      gtcaaatta ttttgtatt tatttgata gagataaga 
      ttctatttt gtacatatg taggtttgt aaaaattat 
      ctcaggatc gttatatcc gtaccaatt gcttgcgaa 
      attgcaata tctaccagc aacaaaatt gcttcttgt 
      actatatca ggtgatact aatacttaa cttagctaa 
      aaaatcaat atgtagctc aatttatac aaaagttag 
      aaatagatg tattctgta acaagtttt gttagctct 
      ctggaacaa gctcaacaa tgtggcgat gttttagcc 
      accttgaag gaattacca aacgtcgtg tgatgcgat 
      ttttacaaa gcaagctaa cactagaaa tttgtacat 
      atgaaatat gtgattttg tgtatattc ttttggtaa 
      aaggtattt caagaaata aactaaccg ctcctgcgg 
      gcgcagcag gggttgagt caagccaaa ttcactaaa 
      cctctcacg cgcgc