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Gene information
General information
TC012373 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5814647..5819939
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012373
    iBeetle screen iB_01972

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012373 RA
      MSDSKETLS SFVDKSAKT TDFDKVLGR NILVAPTEE 
      QFELLLQRL KQQLGEGRG EVILEIGGS EDGSENGLL 
      EDELEAAVA TLQSLATTL ECSCVKLRE RKDVRGIIA 
      QYLIRRVLG QSDFLEIRV AVVGNVDAG KSTLLGVLT 
      HGELDNGRG HARQKLFRH KHEMESGRT SSVGNDILG 
      FDEEGNVVN KPEHGALDW VKICEKSSK VITFIDLAG 
      HERYLKTTV FGMTGHAPD FGMLMVGAN AGIIGMTKE 
      HLGLALALS VPVFVVVTK IDMCPANVL QDNLKMLIK 
      ILKSPGCRK VPVMVKTTD DVILSATNF VSERLCPIF 
      QVSNVSGEN LELLKTFLN LLTTRMEYH ENEPAEFQI 
      DDTYSVPGV GTVVSGTTL KGVIRLNDT LMLGPDPLG 
      HFQQIAVKS IHRKRMAVK EVRAGQTAS FALKKIKRS 
      QIRKGMVMV SPSLNPQAC WEFEGEILV LHHPTTISS 
      RYQAMVHCG SIRQTASIV RMSKDCLRT GDKAIIHFR 
      FIKHPEYMT PGQRMVFRE GRTKAVGNI VRIITQTTP 
      THPNTRTKQ NKQQRYGGG NTQTNHVTK NVQSGLQEF 
      SEIGTAVGE TTFEGSTDK REHSGQKSG SRKGRGRRG 
      GRGKNNNAN SNTAKPLTE TNINSISNL NSTKVQ
    CDS sequence
    • >TC012373 RA
      atgagtgac agtaaagaa acattgagt tcgtttgtg 
      gacaaatcg gcgaaaacc accgacttt gacaaagtc 
      ctaggccgc aacattctg gtggcccca accgaggag 
      cagttcgag ttgctactc cagcggctg aagcagcag 
      ctgggcgaa ggccggggc gaagtcatt ctggagatt 
      gggggctcg gaggacggg agcgaaaac ggcctgctt 
      gaggacgag ctggaggcg gctgtcgcg accctgcag 
      tccttggcc actacttta gaatgcagt tgtgttaag 
      ttgagggag cggaaggac gtcagaggg atcatagcc 
      cagtatttg atccggcgg gtcttaggg cagtcagac 
      tttttggag attcgtgtg gccgtggtc ggtaatgta 
      gatgccggt aaatcgacg ctgttgggt gtcctcacc 
      catggggag ctggacaat gggcgaggg cacgcgaga 
      cagaagctc ttcaggcat aagcacgag atggagtcg 
      ggacggacg agttctgtt ggcaacgat attctgggt 
      ttcgacgaa gagggcaat gtggtgaat aagccagag 
      catggagcg ctcgattgg gtgaaaatc tgcgagaag 
      agctcaaag gtgataaca tttatcgat ttggcggga 
      catgagcgt tatttgaag actacggtg tttggtatg 
      actgggcat gcgcccgat tttgggatg ttgatggtg 
      ggtgcgaat gctggtatt attggcatg actaaggag 
      catctagga ttggctctg gcactgtca gttcctgtg 
      tttgtggtg gttacgaaa attgatatg tgtccggct 
      aatgtgcta caagacaat ttgaaaatg ttgatcaag 
      attttaaaa tcgccaggt tgtcgaaag gtccctgtg 
      atggttaaa acgaccgat gatgtcatc ttaagtgct 
      accaacttc gtatcagaa cgcctgtgc cccattttt 
      caagtctcc aacgtttcc ggagaaaat ctggaatta 
      ttaaagaca tttcttaat ttattgacc acaagaatg 
      gaatatcac gagaacgaa ccagcggag ttccaaatt 
      gacgacact tactcagtt ccgggtgtc ggaactgtg 
      gtttcagga accacactg aaaggagtg attcgttta 
      aatgacacc ttgatgtta ggcccagat cccttgggc 
      cacttccaa caaatcgcc gttaaaagt attcatagg 
      aaacgaatg gccgttaag gaagtgcgt gcgggccag 
      acggcgagt ttcgcgtta aaaaaaatt aaacgatct 
      caaattcgt aaaggcatg gtcatggtt tccccctct 
      ctaaacccg caagcttgc tgggaattc gagggcgaa 
      attctcgtc ttgcatcac cccaccact atcagttca 
      cgttatcaa gccatggtt cattgcgga agcatccga 
      caaactgcg agtatcgtc cgaatgtct aaagattgt 
      ctcagaaca ggcgataaa gcaataatt cacttccgt 
      tttatcaaa cacccggag tacatgact ccgggtcaa 
      agaatggtg ttccgtgaa ggtcgcact aaagcagtt 
      ggcaacatt gtgcgaatt ataacgcaa acaacgccc 
      acgcatccc aatacaagg acgaaacaa aacaagcaa 
      caaagatac ggcggggga aacacacaa accaaccac 
      gtgaccaaa aacgttcag tccggttta caagagttt 
      tcagagatt ggtaccgcc gttggggag acaacgttt 
      gagggaagt accgataag cgtgagcac agtggccaa 
      aaatccggc tcgaggaaa ggtcgaggt aggcgcgga 
      ggtcgtggt aaaaacaat aatgccaac tcaaacacc 
      gcaaaacct ctcactgag actaacatc aattcgatt 
      tcaaacctc aattcgaca aaagtgcaa taa
    mRNA sequence
    • >TC012373 RA
      tgacgtaac atatgtgac tgacatttc gtggtcaca 
      aatttagca gttttttaa atagcgtca ggaatgcaa 
      atcagaaga gtaaacacg aattactct ggttgcaaa 
      ttttggacg ctaatgctc gcgcgataa gagaaacat 
      gagtgacag taaagaaac attgagttc gtttgtgga 
      caaatcggc gaaaaccac cgactttga caaagtcct 
      aggccgcaa cattctggt ggccccaac cgaggagca 
      gttcgagtt gctactcca gcggctgaa gcagcagct 
      gggcgaagg ccggggcga agtcattct ggagattgg 
      gggctcgga ggacgggag cgaaaacgg cctgcttga 
      ggacgagct ggaggcggc tgtcgcgac cctgcagtc 
      cttggccac tactttaga atgcagttg tgttaagtt 
      gagggagcg gaaggacgt cagagggat catagccca 
      gtatttgat ccggcgggt cttagggca gtcagactt 
      tttggagat tcgtgtggc cgtggtcgg taatgtaga 
      tgccggtaa atcgacgct gttgggtgt cctcaccca 
      tggggagct ggacaatgg gcgagggca cgcgagaca 
      gaagctctt caggcataa gcacgagat ggagtcggg 
      acggacgag ttctgttgg caacgatat tctgggttt 
      cgacgaaga gggcaatgt ggtgaataa gccagagca 
      tggagcgct cgattgggt gaaaatctg cgagaagag 
      ctcaaaggt gataacatt tatcgattt ggcgggaca 
      tgagcgtta tttgaagac tacggtgtt tggtatgac 
      tgggcatgc gcccgattt tgggatgtt gatggtggg 
      tgcgaatgc tggtattat tggcatgac taaggagca 
      tctaggatt ggctctggc actgtcagt tcctgtgtt 
      tgtggtggt tacgaaaat tgatatgtg tccggctaa 
      tgtgctaca agacaattt gaaaatgtt gatcaagat 
      tttaaaatc gccaggttg tcgaaaggt ccctgtgat 
      ggttaaaac gaccgatga tgtcatctt aagtgctac 
      caacttcgt atcagaacg cctgtgccc catttttca 
      agtctccaa cgtttccgg agaaaatct ggaattatt 
      aaagacatt tcttaattt attgaccac aagaatgga 
      atatcacga gaacgaacc agcggagtt ccaaattga 
      cgacactta ctcagttcc gggtgtcgg aactgtggt 
      ttcaggaac cacactgaa aggagtgat tcgtttaaa 
      tgacacctt gatgttagg cccagatcc cttgggcca 
      cttccaaca aatcgccgt taaaagtat tcataggaa 
      acgaatggc cgttaagga agtgcgtgc gggccagac 
      ggcgagttt cgcgttaaa aaaaattaa acgatctca 
      aattcgtaa aggcatggt catggtttc cccctctct 
      aaacccgca agcttgctg ggaattcga gggcgaaat 
      tctcgtctt gcatcaccc caccactat cagttcacg 
      ttatcaagc catggttca ttgcggaag catccgaca 
      aactgcgag tatcgtccg aatgtctaa agattgtct 
      cagaacagg cgataaagc aataattca cttccgttt 
      tatcaaaca cccggagta catgactcc gggtcaaag 
      aatggtgtt ccgtgaagg tcgcactaa agcagttgg 
      caacattgt gcgaattat aacgcaaac aacgcccac 
      gcatcccaa tacaaggac gaaacaaaa caagcaaca 
      aagatacgg cgggggaaa cacacaaac caaccacgt 
      gaccaaaaa cgttcagtc cggtttaca agagttttc 
      agagattgg taccgccgt tggggagac aacgtttga 
      gggaagtac cgataagcg tgagcacag tggccaaaa 
      atccggctc gaggaaagg tcgaggtag gcgcggagg 
      tcgtggtaa aaacaataa tgccaactc aaacaccgc 
      aaaacctct cactgagac taacatcaa ttcgatttc 
      aaacctcaa ttcgacaaa agtgcaata atcgcgcaa 
      attctttcg tttttaact aagtccgag tgagaaaaa 
      ggcagaaat aaagtaatt tattttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY