Status
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Gene information
General information
TC034303
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012358
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5557436..5567865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034303
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01968

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    abdomen posterior remaining - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034303 RA
      MCLLKVIFV MVMVNQLEA IQNKATADN TSATEIINN 
      NTSAPALEC DALKATGCL DGTNLNLSL TYWHPTKKP 
      MKWLVNTVG SNSYCNQAR IINNFLIVD TSYINSSCI 
      YNISTIEPD VPHFLIKDS SFKEMDVAN CPKKTEFCV 
      SVGDTFSTS LGIFNTTTL PPPLVCELK ATGCGNGTN 
      ITLNLAWNH LLPEMPSNW VVRRIEPPN CPKVRVFNN 
      SLSIDTAYI NSSCIYDIT TVGKEFISH IEGSVFKEM 
      GMESCPKKK ETCITINSN DNFNISSGE KCPYLHNYS 
      INAMVNNRT NIPYIYLNV SLDLVDANS DKIEIHVKS 
      RHRNSCGFM YDNFHDADH EIIIPKALP VSDITPECE 
      LEILMSFPN ADSCTVSYK VPEIPGFCY EMLNPTSKI 
      LSVEPLYDN QFKILWDPG TKARNNQSY YLKKVNFFY 
      LATETPSMP SEVEFVGKP DLEAHTATV VMKLFENDN 
      YTLQGIFTN NYTCLHQAE FYFTAPPRR SLHLVFFTL 
      LTSVLVVMT FLHKRILRY AKKLLGLVI PRYRNITHR 
      ENFDLQPLQ SGNLVHMSI NTQYTPIEF LRGDFDEFE 
      FPRNKIIIK EILGTGAFG RVYSAKALG IGGSEGYQM 
      VAVKTLGEG EQITREAAD DFKSEIEIF KKIGKHPNI 
      VSLLGCCTV EAPCMMIME LVPCGDLKK YLVELREQW 
      FAEKNKQVF FPPENDNSD GAYIEPSSP TSVNSITTS 
      RLPSTSETV FTNLEDPMT PLLEHNQQA LQKVLDHKE 
      LQNFALQIA RGMAHLEKI SITHRDLAA RNILINEFK 
      TLKISDFGL SRTGVYINQ RTKKLPLRW MALEAITEQ 
      KYDSRSDVW SFGVVLWEI GTLGAFPYE KVPDSMILH 
      FLQMGRRLE RPEICTDEL YSLMRQCWA TDPSQRPTF 
      RELESALDV KAKKVYVNF DQLNPSYVF PPSDARVVK 
      GVPIVINPR IIQEEEEGT
    CDS sequence
    • >TC034303 RA
      atgtgtttg ttgaaagtg atatttgtt atggtcatg 
      gtaaaccaa ttggaagcc atccaaaac aaggcaacg 
      gctgataac acctcagcg acagaaata attaacaat 
      aacacctct gcccccgcg ctcgaatgt gatgctcta 
      aaggcgaca ggatgcctg gatggaacg aatcttaat 
      ctaagtcta acttattgg caccccact aaaaaaccc 
      atgaaatgg ctagttaac actgtagga tcaaacagc 
      tattgtaac caagctaga attatcaat aatttttta 
      attgttgac acgagctat attaattct tcctgtatt 
      tacaatatt tcaacgatt gagccagac gttccgcat 
      tttctgata aaagactcg agtttcaaa gaaatggac 
      gtggcaaat tgccccaaa aaaacggaa ttttgcgtc 
      agtgtcggc gatactttt agcacgagt ctaggaata 
      tttaacaca accaccctt ccgcctcca ctcgtatgt 
      gaattaaaa gcgacagga tgtggtaat ggaactaat 
      attactctt aatttagct tggaaccac ctgttacct 
      gaaatgcca agtaattgg gtagttcgt aggatagaa 
      ccaccaaac tgtcctaaa gttagagta ttcaataat 
      tcattaagt attgatacc gcctatatt aattcttct 
      tgtatttat gacattaca acagtcggt aaagaattc 
      atttcacat atagaagga tcggtattc aaagaaatg 
      ggcatggag tcatgtccc aagaaaaaa gaaacgtgt 
      atcactatc aatagcaat gataatttt aatataagc 
      tcaggagaa aagtgtccc tacttacac aactattcc 
      ataaatgcg atggtgaac aacagaacg aacatcccg 
      tacatttat ttgaacgta agtttggat cttgttgat 
      gcaaacagc gacaaaatc gagatccat gtgaagtca 
      agacatagg aacagttgc ggattcatg tatgacaac 
      ttccacgac gccgatcac gaaattatc ataccgaag 
      gcattgccg gttagcgac attacaccc gagtgcgag 
      cttgagatc ttgatgtcc ttccccaac gctgattcc 
      tgcaccgtc tcatacaaa gtaccagaa atcccaggt 
      ttctgctac gagatgctc aacccaact tcgaaaatt 
      ttaagcgtg gagccactt tacgacaac cagtttaaa 
      attctctgg gaccctggc accaaagcc aggaacaac 
      cagtcctat tacctgaaa aaagtcaac tttttttac 
      ttagctacg gaaacgcct tcgatgccc agtgaagtc 
      gagtttgtt ggaaaacca gacctggag gcacacaca 
      gctacggtg gttatgaag ctttttgag aatgacaat 
      tacacgctg caggggatt ttcaccaat aattacacc 
      tgtttacac caagccgaa ttttacttc acagctcct 
      cctcgccga agcttacat ctggtgttt tttaccctt 
      ctgaccagc gttctagtc gtgatgact ttcctgcat 
      aagcgtatt ttgcggtat gcgaaaaag ttgctaggg 
      ctggtaatc ccaagatac aggaacata actcaccga 
      gagaatttt gatttacaa ccgttgcaa agtgggaac 
      cttgttcac atgagcatc aacactcaa tacactcca 
      atcgaattt ttgagaggc gatttcgac gaattcgaa 
      ttcccccgg aacaagata atcattaag gaaatcctc 
      ggaactgga gcttttggc cgagtgtat tcagccaaa 
      gctctggga attggcgga agtgagggt taccaaatg 
      gtggcggtt aaaacgtta ggggaagga gagcaaata 
      acaagagag gcagccgat gatttcaag tcggaaatt 
      gagatattc aaaaaaatt ggtaagcac cccaatatt 
      gtgtcgctc ctgggttgt tgtactgtg gaagcaccc 
      tgtatgatg ataatggag ttggtgccg tgtggcgac 
      ctcaagaaa tatcttgtg gagttgcgg gaacaatgg 
      tttgcggaa aaaaataaa caagtgttt tttccccca 
      gagaacgac aatagtgat ggggcatat attgaacca 
      tcgagccct accagtgtt aattctata accacaagc 
      aggttacca tcgacttcc gaaactgtc tttacgaat 
      ctggaggac cccatgacg cccctcctc gagcataac 
      cagcaggcc cttcagaag gttttggac cacaaggag 
      ctccagaac ttcgccctg caaatcgcg cggggaatg 
      gcccatctg gagaagatc tccatcacc caccgcgac 
      ctcgccgct cgcaacatc ctaatcaac gagttcaaa 
      actttgaaa atctccgac ttcggactg tcccgaacc 
      ggggtgtac atcaaccaa aggacgaaa aaactcccc 
      ctgcgctgg atggctctg gaagccatc accgagcag 
      aagtacgac agtagaagt gacgtttgg tctttcggt 
      gtagtgttg tgggagatt ggcacactt ggtgcgttc 
      ccgtacgaa aaagtgccc gattcgatg attctgcac 
      ttcctgcag atggggcgg aggctggaa aggccggag 
      atctgcact gatgaactt tattcgctg atgaggcag 
      tgctgggcc acggacccg agtcaaagg cccacgttt 
      agagaactg gagagtgcg ctggatgtt aaggcgaag 
      aaggtttac gtgaacttt gaccaactt aatccgtct 
      tatgttttt ccgccgagt gatgcgcgt gtggtgaaa 
      ggggtgccc attgttatt aatccgagg ataattcag 
      gaggaagaa gagggaact tga
    mRNA sequence
    • >TC034303 RA
      gttaattga ccaagcgtg agctaatcg cggaaactt 
      gtgttattt tgatttgtg tgtgattaa ttcaacact 
      tagtttgag gattatata attttttcg agtgtgtgt 
      ccgattcta aatggtttt cgcatccgt gaatattgg 
      gcaagacac atgatttga ttagaaatt ataaatcga 
      taagatagc attgaaagt ttgtcaaat aagatgatc 
      gcatgttcg agaatagtt tttctcata agccatctg 
      tgcaatgtg aatcctagt caaagtttt caatttacc 
      tataaatct cctcaaatt cctaaacag tggttctag 
      acgatttta ttttatttt tatcatgga ctcttagac 
      gggagttgt tagggtcca gtcttttcc ggaagcggt 
      aaaaaatgt gtttgttga aagtgatat ttgttatgg 
      tcatggtaa accaattgg aagccatcc aaaacaagg 
      caacggctg ataacacct cagcgacag aaataatta 
      acaataaca cctctgccc ccgcgctcg aatgtgatg 
      ctctaaagg cgacaggat gcctggatg gaacgaatc 
      ttaatctaa gtctaactt attggcacc ccactaaaa 
      aacccatga aatggctag ttaacactg taggatcaa 
      acagctatt gtaaccaag ctagaatta tcaataatt 
      ttttaattg ttgacacga gctatatta attcttcct 
      gtatttaca atatttcaa cgattgagc cagacgttc 
      cgcattttc tgataaaag actcgagtt tcaaagaaa 
      tggacgtgg caaattgcc ccaaaaaaa cggaatttt 
      gcgtcagtg tcggcgata cttttagca cgagtctag 
      gaatattta acacaacca cccttccgc ctccactcg 
      tatgtgaat taaaagcga caggatgtg gtaatggaa 
      ctaatatta ctcttaatt tagcttgga accacctgt 
      tacctgaaa tgccaagta attgggtag ttcgtagga 
      tagaaccac caaactgtc ctaaagtta gagtattca 
      ataattcat taagtattg ataccgcct atattaatt 
      cttcttgta tttatgaca ttacaacag tcggtaaag 
      aattcattt cacatatag aaggatcgg tattcaaag 
      aaatgggca tggagtcat gtcccaaga aaaaagaaa 
      cgtgtatca ctatcaata gcaatgata attttaata 
      taagctcag gagaaaagt gtccctact tacacaact 
      attccataa atgcgatgg tgaacaaca gaacgaaca 
      tcccgtaca tttatttga acgtaagtt tggatcttg 
      ttgatgcaa acagcgaca aaatcgaga tccatgtga 
      agtcaagac ataggaaca gttgcggat tcatgtatg 
      acaacttcc acgacgccg atcacgaaa ttatcatac 
      cgaaggcat tgccggtta gcgacatta cacccgagt 
      gcgagcttg agatcttga tgtccttcc ccaacgctg 
      attcctgca ccgtctcat acaaagtac cagaaatcc 
      caggtttct gctacgaga tgctcaacc caacttcga 
      aaattttaa gcgtggagc cactttacg acaaccagt 
      ttaaaattc tctgggacc ctggcacca aagccagga 
      acaaccagt cctattacc tgaaaaaag tcaactttt 
      tttacttag ctacggaaa cgccttcga tgcccagtg 
      aagtcgagt ttgttggaa aaccagacc tggaggcac 
      acacagcta cggtggtta tgaagcttt ttgagaatg 
      acaattaca cgctgcagg ggattttca ccaataatt 
      acacctgtt tacaccaag ccgaatttt acttcacag 
      ctcctcctc gccgaagct tacatctgg tgtttttta 
      cccttctga ccagcgttc tagtcgtga tgactttcc 
      tgcataagc gtattttgc ggtatgcga aaaagttgc 
      tagggctgg taatcccaa gatacagga acataactc 
      accgagaga attttgatt tacaaccgt tgcaaagtg 
      ggaaccttg ttcacatga gcatcaaca ctcaataca 
      ctccaatcg aatttttga gaggcgatt tcgacgaat 
      tcgaattcc cccggaaca agataatca ttaaggaaa 
      tcctcggaa ctggagctt ttggccgag tgtattcag 
      ccaaagctc tgggaattg gcggaagtg agggttacc 
      aaatggtgg cggttaaaa cgttagggg aaggagagc 
      aaataacaa gagaggcag ccgatgatt tcaagtcgg 
      aaattgaga tattcaaaa aaattggta agcacccca 
      atattgtgt cgctcctgg gttgttgta ctgtggaag 
      caccctgta tgatgataa tggagttgg tgccgtgtg 
      gcgacctca agaaatatc ttgtggagt tgcgggaac 
      aatggtttg cggaaaaaa ataaacaag tgttttttc 
      ccccagaga acgacaata gtgatgggg catatattg 
      aaccatcga gccctacca gtgttaatt ctataacca 
      caagcaggt taccatcga cttccgaaa ctgtcttta 
      cgaatctgg aggacccca tgacgcccc tcctcgagc 
      ataaccagc aggcccttc agaaggttt tggaccaca 
      aggagctcc agaacttcg ccctgcaaa tcgcgcggg 
      gaatggccc atctggaga agatctcca tcacccacc 
      gcgacctcg ccgctcgca acatcctaa tcaacgagt 
      tcaaaactt tgaaaatct ccgacttcg gactgtccc 
      gaaccgggg tgtacatca accaaagga cgaaaaaac 
      tccccctgc gctggatgg ctctggaag ccatcaccg 
      agcagaagt acgacagta gaagtgacg tttggtctt 
      tcggtgtag tgttgtggg agattggca cacttggtg 
      cgttcccgt acgaaaaag tgcccgatt cgatgattc 
      tgcacttcc tgcagatgg ggcggaggc tggaaaggc 
      cggagatct gcactgatg aactttatt cgctgatga 
      ggcagtgct gggccacgg acccgagtc aaaggccca 
      cgtttagag aactggaga gtgcgctgg atgttaagg 
      cgaagaagg tttacgtga actttgacc aacttaatc 
      cgtcttatg tttttccgc cgagtgatg cgcgtgtgg 
      tgaaagggg tgcccattg ttattaatc cgaggataa 
      ttcaggagg aagaagagg gaacttgat gataataaa 
      attatcgtg atgtgtgat atttcaaaa ttaaacgca 
      ctttgttta tttatttcg taaagttaa ggcacccac 
      tcgtatttg ccatttttc acgcgaaaa aagacgaat 
      ttctgttga aaataaata atttattgt gctttgttt 
      tttctatga aaaaataca tgttttcca ttattaata 
      aattgattt tcatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY