Status
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Gene information
General information
TC034303
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012358 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5557436..5567865
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034303
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01968

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     abdomen posterior remaining - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034303 RA
      MCLLKVIFV MVMVNQLEA IQNKATADN TSATEIINN 
NTSAPALEC DALKATGCL DGTNLNLSL TYWHPTKKP 
MKWLVNTVG SNSYCNQAR IINNFLIVD TSYINSSCI 
YNISTIEPD VPHFLIKDS SFKEMDVAN CPKKTEFCV 
SVGDTFSTS LGIFNTTTL PPPLVCELK ATGCGNGTN 
ITLNLAWNH LLPEMPSNW VVRRIEPPN CPKVRVFNN 
SLSIDTAYI NSSCIYDIT TVGKEFISH IEGSVFKEM 
GMESCPKKK ETCITINSN DNFNISSGE KCPYLHNYS 
INAMVNNRT NIPYIYLNV SLDLVDANS DKIEIHVKS 
RHRNSCGFM YDNFHDADH EIIIPKALP VSDITPECE 
LEILMSFPN ADSCTVSYK VPEIPGFCY EMLNPTSKI 
LSVEPLYDN QFKILWDPG TKARNNQSY YLKKVNFFY 
LATETPSMP SEVEFVGKP DLEAHTATV VMKLFENDN 
YTLQGIFTN NYTCLHQAE FYFTAPPRR SLHLVFFTL 
LTSVLVVMT FLHKRILRY AKKLLGLVI PRYRNITHR 
ENFDLQPLQ SGNLVHMSI NTQYTPIEF LRGDFDEFE 
FPRNKIIIK EILGTGAFG RVYSAKALG IGGSEGYQM 
VAVKTLGEG EQITREAAD DFKSEIEIF KKIGKHPNI 
VSLLGCCTV EAPCMMIME LVPCGDLKK YLVELREQW 
FAEKNKQVF FPPENDNSD GAYIEPSSP TSVNSITTS 
RLPSTSETV FTNLEDPMT PLLEHNQQA LQKVLDHKE 
LQNFALQIA RGMAHLEKI SITHRDLAA RNILINEFK 
TLKISDFGL SRTGVYINQ RTKKLPLRW MALEAITEQ 
KYDSRSDVW SFGVVLWEI GTLGAFPYE KVPDSMILH 
FLQMGRRLE RPEICTDEL YSLMRQCWA TDPSQRPTF 
RELESALDV KAKKVYVNF DQLNPSYVF PPSDARVVK 
GVPIVINPR IIQEEEEGT
    CDS sequence
    • >TC034303 RA
      atgtgtttg ttgaaagtg atatttgtt atggtcatg 
gtaaaccaa ttggaagcc atccaaaac aaggcaacg 
gctgataac acctcagcg acagaaata attaacaat 
aacacctct gcccccgcg ctcgaatgt gatgctcta 
aaggcgaca ggatgcctg gatggaacg aatcttaat 
ctaagtcta acttattgg caccccact aaaaaaccc 
atgaaatgg ctagttaac actgtagga tcaaacagc 
tattgtaac caagctaga attatcaat aatttttta 
attgttgac acgagctat attaattct tcctgtatt 
tacaatatt tcaacgatt gagccagac gttccgcat 
tttctgata aaagactcg agtttcaaa gaaatggac 
gtggcaaat tgccccaaa aaaacggaa ttttgcgtc 
agtgtcggc gatactttt agcacgagt ctaggaata 
tttaacaca accaccctt ccgcctcca ctcgtatgt 
gaattaaaa gcgacagga tgtggtaat ggaactaat 
attactctt aatttagct tggaaccac ctgttacct 
gaaatgcca agtaattgg gtagttcgt aggatagaa 
ccaccaaac tgtcctaaa gttagagta ttcaataat 
tcattaagt attgatacc gcctatatt aattcttct 
tgtatttat gacattaca acagtcggt aaagaattc 
atttcacat atagaagga tcggtattc aaagaaatg 
ggcatggag tcatgtccc aagaaaaaa gaaacgtgt 
atcactatc aatagcaat gataatttt aatataagc 
tcaggagaa aagtgtccc tacttacac aactattcc 
ataaatgcg atggtgaac aacagaacg aacatcccg 
tacatttat ttgaacgta agtttggat cttgttgat 
gcaaacagc gacaaaatc gagatccat gtgaagtca 
agacatagg aacagttgc ggattcatg tatgacaac 
ttccacgac gccgatcac gaaattatc ataccgaag 
gcattgccg gttagcgac attacaccc gagtgcgag 
cttgagatc ttgatgtcc ttccccaac gctgattcc 
tgcaccgtc tcatacaaa gtaccagaa atcccaggt 
ttctgctac gagatgctc aacccaact tcgaaaatt 
ttaagcgtg gagccactt tacgacaac cagtttaaa 
attctctgg gaccctggc accaaagcc aggaacaac 
cagtcctat tacctgaaa aaagtcaac tttttttac 
ttagctacg gaaacgcct tcgatgccc agtgaagtc 
gagtttgtt ggaaaacca gacctggag gcacacaca 
gctacggtg gttatgaag ctttttgag aatgacaat 
tacacgctg caggggatt ttcaccaat aattacacc 
tgtttacac caagccgaa ttttacttc acagctcct 
cctcgccga agcttacat ctggtgttt tttaccctt 
ctgaccagc gttctagtc gtgatgact ttcctgcat 
aagcgtatt ttgcggtat gcgaaaaag ttgctaggg 
ctggtaatc ccaagatac aggaacata actcaccga 
gagaatttt gatttacaa ccgttgcaa agtgggaac 
cttgttcac atgagcatc aacactcaa tacactcca 
atcgaattt ttgagaggc gatttcgac gaattcgaa 
ttcccccgg aacaagata atcattaag gaaatcctc 
ggaactgga gcttttggc cgagtgtat tcagccaaa 
gctctggga attggcgga agtgagggt taccaaatg 
gtggcggtt aaaacgtta ggggaagga gagcaaata 
acaagagag gcagccgat gatttcaag tcggaaatt 
gagatattc aaaaaaatt ggtaagcac cccaatatt 
gtgtcgctc ctgggttgt tgtactgtg gaagcaccc 
tgtatgatg ataatggag ttggtgccg tgtggcgac 
ctcaagaaa tatcttgtg gagttgcgg gaacaatgg 
tttgcggaa aaaaataaa caagtgttt tttccccca 
gagaacgac aatagtgat ggggcatat attgaacca 
tcgagccct accagtgtt aattctata accacaagc 
aggttacca tcgacttcc gaaactgtc tttacgaat 
ctggaggac cccatgacg cccctcctc gagcataac 
cagcaggcc cttcagaag gttttggac cacaaggag 
ctccagaac ttcgccctg caaatcgcg cggggaatg 
gcccatctg gagaagatc tccatcacc caccgcgac 
ctcgccgct cgcaacatc ctaatcaac gagttcaaa 
actttgaaa atctccgac ttcggactg tcccgaacc 
ggggtgtac atcaaccaa aggacgaaa aaactcccc 
ctgcgctgg atggctctg gaagccatc accgagcag 
aagtacgac agtagaagt gacgtttgg tctttcggt 
gtagtgttg tgggagatt ggcacactt ggtgcgttc 
ccgtacgaa aaagtgccc gattcgatg attctgcac 
ttcctgcag atggggcgg aggctggaa aggccggag 
atctgcact gatgaactt tattcgctg atgaggcag 
tgctgggcc acggacccg agtcaaagg cccacgttt 
agagaactg gagagtgcg ctggatgtt aaggcgaag 
aaggtttac gtgaacttt gaccaactt aatccgtct 
tatgttttt ccgccgagt gatgcgcgt gtggtgaaa 
ggggtgccc attgttatt aatccgagg ataattcag 
gaggaagaa gagggaact tga
    mRNA sequence
    • >TC034303 RA
      gttaattga ccaagcgtg agctaatcg cggaaactt 
gtgttattt tgatttgtg tgtgattaa ttcaacact 
tagtttgag gattatata attttttcg agtgtgtgt 
ccgattcta aatggtttt cgcatccgt gaatattgg 
gcaagacac atgatttga ttagaaatt ataaatcga 
taagatagc attgaaagt ttgtcaaat aagatgatc 
gcatgttcg agaatagtt tttctcata agccatctg 
tgcaatgtg aatcctagt caaagtttt caatttacc 
tataaatct cctcaaatt cctaaacag tggttctag 
acgatttta ttttatttt tatcatgga ctcttagac 
gggagttgt tagggtcca gtcttttcc ggaagcggt 
aaaaaatgt gtttgttga aagtgatat ttgttatgg 
tcatggtaa accaattgg aagccatcc aaaacaagg 
caacggctg ataacacct cagcgacag aaataatta 
acaataaca cctctgccc ccgcgctcg aatgtgatg 
ctctaaagg cgacaggat gcctggatg gaacgaatc 
ttaatctaa gtctaactt attggcacc ccactaaaa 
aacccatga aatggctag ttaacactg taggatcaa 
acagctatt gtaaccaag ctagaatta tcaataatt 
ttttaattg ttgacacga gctatatta attcttcct 
gtatttaca atatttcaa cgattgagc cagacgttc 
cgcattttc tgataaaag actcgagtt tcaaagaaa 
tggacgtgg caaattgcc ccaaaaaaa cggaatttt 
gcgtcagtg tcggcgata cttttagca cgagtctag 
gaatattta acacaacca cccttccgc ctccactcg 
tatgtgaat taaaagcga caggatgtg gtaatggaa 
ctaatatta ctcttaatt tagcttgga accacctgt 
tacctgaaa tgccaagta attgggtag ttcgtagga 
tagaaccac caaactgtc ctaaagtta gagtattca 
ataattcat taagtattg ataccgcct atattaatt 
cttcttgta tttatgaca ttacaacag tcggtaaag 
aattcattt cacatatag aaggatcgg tattcaaag 
aaatgggca tggagtcat gtcccaaga aaaaagaaa 
cgtgtatca ctatcaata gcaatgata attttaata 
taagctcag gagaaaagt gtccctact tacacaact 
attccataa atgcgatgg tgaacaaca gaacgaaca 
tcccgtaca tttatttga acgtaagtt tggatcttg 
ttgatgcaa acagcgaca aaatcgaga tccatgtga 
agtcaagac ataggaaca gttgcggat tcatgtatg 
acaacttcc acgacgccg atcacgaaa ttatcatac 
cgaaggcat tgccggtta gcgacatta cacccgagt 
gcgagcttg agatcttga tgtccttcc ccaacgctg 
attcctgca ccgtctcat acaaagtac cagaaatcc 
caggtttct gctacgaga tgctcaacc caacttcga 
aaattttaa gcgtggagc cactttacg acaaccagt 
ttaaaattc tctgggacc ctggcacca aagccagga 
acaaccagt cctattacc tgaaaaaag tcaactttt 
tttacttag ctacggaaa cgccttcga tgcccagtg 
aagtcgagt ttgttggaa aaccagacc tggaggcac 
acacagcta cggtggtta tgaagcttt ttgagaatg 
acaattaca cgctgcagg ggattttca ccaataatt 
acacctgtt tacaccaag ccgaatttt acttcacag 
ctcctcctc gccgaagct tacatctgg tgtttttta 
cccttctga ccagcgttc tagtcgtga tgactttcc 
tgcataagc gtattttgc ggtatgcga aaaagttgc 
tagggctgg taatcccaa gatacagga acataactc 
accgagaga attttgatt tacaaccgt tgcaaagtg 
ggaaccttg ttcacatga gcatcaaca ctcaataca 
ctccaatcg aatttttga gaggcgatt tcgacgaat 
tcgaattcc cccggaaca agataatca ttaaggaaa 
tcctcggaa ctggagctt ttggccgag tgtattcag 
ccaaagctc tgggaattg gcggaagtg agggttacc 
aaatggtgg cggttaaaa cgttagggg aaggagagc 
aaataacaa gagaggcag ccgatgatt tcaagtcgg 
aaattgaga tattcaaaa aaattggta agcacccca 
atattgtgt cgctcctgg gttgttgta ctgtggaag 
caccctgta tgatgataa tggagttgg tgccgtgtg 
gcgacctca agaaatatc ttgtggagt tgcgggaac 
aatggtttg cggaaaaaa ataaacaag tgttttttc 
ccccagaga acgacaata gtgatgggg catatattg 
aaccatcga gccctacca gtgttaatt ctataacca 
caagcaggt taccatcga cttccgaaa ctgtcttta 
cgaatctgg aggacccca tgacgcccc tcctcgagc 
ataaccagc aggcccttc agaaggttt tggaccaca 
aggagctcc agaacttcg ccctgcaaa tcgcgcggg 
gaatggccc atctggaga agatctcca tcacccacc 
gcgacctcg ccgctcgca acatcctaa tcaacgagt 
tcaaaactt tgaaaatct ccgacttcg gactgtccc 
gaaccgggg tgtacatca accaaagga cgaaaaaac 
tccccctgc gctggatgg ctctggaag ccatcaccg 
agcagaagt acgacagta gaagtgacg tttggtctt 
tcggtgtag tgttgtggg agattggca cacttggtg 
cgttcccgt acgaaaaag tgcccgatt cgatgattc 
tgcacttcc tgcagatgg ggcggaggc tggaaaggc 
cggagatct gcactgatg aactttatt cgctgatga 
ggcagtgct gggccacgg acccgagtc aaaggccca 
cgtttagag aactggaga gtgcgctgg atgttaagg 
cgaagaagg tttacgtga actttgacc aacttaatc 
cgtcttatg tttttccgc cgagtgatg cgcgtgtgg 
tgaaagggg tgcccattg ttattaatc cgaggataa 
ttcaggagg aagaagagg gaacttgat gataataaa 
attatcgtg atgtgtgat atttcaaaa ttaaacgca 
ctttgttta tttatttcg taaagttaa ggcacccac 
tcgtatttg ccatttttc acgcgaaaa aagacgaat 
ttctgttga aaataaata atttattgt gctttgttt 
tttctatga aaaaataca tgttttcca ttattaata 
aattgattt tcatttt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY