General information | |||
TC034303
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 5557436..5567865 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034303 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01968 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)abdomen posterior remaining - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC034303 RA
MCLLKVIFV MVMVNQLEA IQNKATADN TSATEIINN NTSAPALEC DALKATGCL DGTNLNLSL TYWHPTKKP MKWLVNTVG SNSYCNQAR IINNFLIVD TSYINSSCI YNISTIEPD VPHFLIKDS SFKEMDVAN CPKKTEFCV SVGDTFSTS LGIFNTTTL PPPLVCELK ATGCGNGTN ITLNLAWNH LLPEMPSNW VVRRIEPPN CPKVRVFNN SLSIDTAYI NSSCIYDIT TVGKEFISH IEGSVFKEM GMESCPKKK ETCITINSN DNFNISSGE KCPYLHNYS INAMVNNRT NIPYIYLNV SLDLVDANS DKIEIHVKS RHRNSCGFM YDNFHDADH EIIIPKALP VSDITPECE LEILMSFPN ADSCTVSYK VPEIPGFCY EMLNPTSKI LSVEPLYDN QFKILWDPG TKARNNQSY YLKKVNFFY LATETPSMP SEVEFVGKP DLEAHTATV VMKLFENDN YTLQGIFTN NYTCLHQAE FYFTAPPRR SLHLVFFTL LTSVLVVMT FLHKRILRY AKKLLGLVI PRYRNITHR ENFDLQPLQ SGNLVHMSI NTQYTPIEF LRGDFDEFE FPRNKIIIK EILGTGAFG RVYSAKALG IGGSEGYQM VAVKTLGEG EQITREAAD DFKSEIEIF KKIGKHPNI VSLLGCCTV EAPCMMIME LVPCGDLKK YLVELREQW FAEKNKQVF FPPENDNSD GAYIEPSSP TSVNSITTS RLPSTSETV FTNLEDPMT PLLEHNQQA LQKVLDHKE LQNFALQIA RGMAHLEKI SITHRDLAA RNILINEFK TLKISDFGL SRTGVYINQ RTKKLPLRW MALEAITEQ KYDSRSDVW SFGVVLWEI GTLGAFPYE KVPDSMILH FLQMGRRLE RPEICTDEL YSLMRQCWA TDPSQRPTF RELESALDV KAKKVYVNF DQLNPSYVF PPSDARVVK GVPIVINPR IIQEEEEGT
- >TC034303 RA
atgtgtttg ttgaaagtg atatttgtt atggtcatg gtaaaccaa ttggaagcc atccaaaac aaggcaacg gctgataac acctcagcg acagaaata attaacaat aacacctct gcccccgcg ctcgaatgt gatgctcta aaggcgaca ggatgcctg gatggaacg aatcttaat ctaagtcta acttattgg caccccact aaaaaaccc atgaaatgg ctagttaac actgtagga tcaaacagc tattgtaac caagctaga attatcaat aatttttta attgttgac acgagctat attaattct tcctgtatt tacaatatt tcaacgatt gagccagac gttccgcat tttctgata aaagactcg agtttcaaa gaaatggac gtggcaaat tgccccaaa aaaacggaa ttttgcgtc agtgtcggc gatactttt agcacgagt ctaggaata tttaacaca accaccctt ccgcctcca ctcgtatgt gaattaaaa gcgacagga tgtggtaat ggaactaat attactctt aatttagct tggaaccac ctgttacct gaaatgcca agtaattgg gtagttcgt aggatagaa ccaccaaac tgtcctaaa gttagagta ttcaataat tcattaagt attgatacc gcctatatt aattcttct tgtatttat gacattaca acagtcggt aaagaattc atttcacat atagaagga tcggtattc aaagaaatg ggcatggag tcatgtccc aagaaaaaa gaaacgtgt atcactatc aatagcaat gataatttt aatataagc tcaggagaa aagtgtccc tacttacac aactattcc ataaatgcg atggtgaac aacagaacg aacatcccg tacatttat ttgaacgta agtttggat cttgttgat gcaaacagc gacaaaatc gagatccat gtgaagtca agacatagg aacagttgc ggattcatg tatgacaac ttccacgac gccgatcac gaaattatc ataccgaag gcattgccg gttagcgac attacaccc gagtgcgag cttgagatc ttgatgtcc ttccccaac gctgattcc tgcaccgtc tcatacaaa gtaccagaa atcccaggt ttctgctac gagatgctc aacccaact tcgaaaatt ttaagcgtg gagccactt tacgacaac cagtttaaa attctctgg gaccctggc accaaagcc aggaacaac cagtcctat tacctgaaa aaagtcaac tttttttac ttagctacg gaaacgcct tcgatgccc agtgaagtc gagtttgtt ggaaaacca gacctggag gcacacaca gctacggtg gttatgaag ctttttgag aatgacaat tacacgctg caggggatt ttcaccaat aattacacc tgtttacac caagccgaa ttttacttc acagctcct cctcgccga agcttacat ctggtgttt tttaccctt ctgaccagc gttctagtc gtgatgact ttcctgcat aagcgtatt ttgcggtat gcgaaaaag ttgctaggg ctggtaatc ccaagatac aggaacata actcaccga gagaatttt gatttacaa ccgttgcaa agtgggaac cttgttcac atgagcatc aacactcaa tacactcca atcgaattt ttgagaggc gatttcgac gaattcgaa ttcccccgg aacaagata atcattaag gaaatcctc ggaactgga gcttttggc cgagtgtat tcagccaaa gctctggga attggcgga agtgagggt taccaaatg gtggcggtt aaaacgtta ggggaagga gagcaaata acaagagag gcagccgat gatttcaag tcggaaatt gagatattc aaaaaaatt ggtaagcac cccaatatt gtgtcgctc ctgggttgt tgtactgtg gaagcaccc tgtatgatg ataatggag ttggtgccg tgtggcgac ctcaagaaa tatcttgtg gagttgcgg gaacaatgg tttgcggaa aaaaataaa caagtgttt tttccccca gagaacgac aatagtgat ggggcatat attgaacca tcgagccct accagtgtt aattctata accacaagc aggttacca tcgacttcc gaaactgtc tttacgaat ctggaggac cccatgacg cccctcctc gagcataac cagcaggcc cttcagaag gttttggac cacaaggag ctccagaac ttcgccctg caaatcgcg cggggaatg gcccatctg gagaagatc tccatcacc caccgcgac ctcgccgct cgcaacatc ctaatcaac gagttcaaa actttgaaa atctccgac ttcggactg tcccgaacc ggggtgtac atcaaccaa aggacgaaa aaactcccc ctgcgctgg atggctctg gaagccatc accgagcag aagtacgac agtagaagt gacgtttgg tctttcggt gtagtgttg tgggagatt ggcacactt ggtgcgttc ccgtacgaa aaagtgccc gattcgatg attctgcac ttcctgcag atggggcgg aggctggaa aggccggag atctgcact gatgaactt tattcgctg atgaggcag tgctgggcc acggacccg agtcaaagg cccacgttt agagaactg gagagtgcg ctggatgtt aaggcgaag aaggtttac gtgaacttt gaccaactt aatccgtct tatgttttt ccgccgagt gatgcgcgt gtggtgaaa ggggtgccc attgttatt aatccgagg ataattcag gaggaagaa gagggaact tga
- >TC034303 RA
gttaattga ccaagcgtg agctaatcg cggaaactt gtgttattt tgatttgtg tgtgattaa ttcaacact tagtttgag gattatata attttttcg agtgtgtgt ccgattcta aatggtttt cgcatccgt gaatattgg gcaagacac atgatttga ttagaaatt ataaatcga taagatagc attgaaagt ttgtcaaat aagatgatc gcatgttcg agaatagtt tttctcata agccatctg tgcaatgtg aatcctagt caaagtttt caatttacc tataaatct cctcaaatt cctaaacag tggttctag acgatttta ttttatttt tatcatgga ctcttagac gggagttgt tagggtcca gtcttttcc ggaagcggt aaaaaatgt gtttgttga aagtgatat ttgttatgg tcatggtaa accaattgg aagccatcc aaaacaagg caacggctg ataacacct cagcgacag aaataatta acaataaca cctctgccc ccgcgctcg aatgtgatg ctctaaagg cgacaggat gcctggatg gaacgaatc ttaatctaa gtctaactt attggcacc ccactaaaa aacccatga aatggctag ttaacactg taggatcaa acagctatt gtaaccaag ctagaatta tcaataatt ttttaattg ttgacacga gctatatta attcttcct gtatttaca atatttcaa cgattgagc cagacgttc cgcattttc tgataaaag actcgagtt tcaaagaaa tggacgtgg caaattgcc ccaaaaaaa cggaatttt gcgtcagtg tcggcgata cttttagca cgagtctag gaatattta acacaacca cccttccgc ctccactcg tatgtgaat taaaagcga caggatgtg gtaatggaa ctaatatta ctcttaatt tagcttgga accacctgt tacctgaaa tgccaagta attgggtag ttcgtagga tagaaccac caaactgtc ctaaagtta gagtattca ataattcat taagtattg ataccgcct atattaatt cttcttgta tttatgaca ttacaacag tcggtaaag aattcattt cacatatag aaggatcgg tattcaaag aaatgggca tggagtcat gtcccaaga aaaaagaaa cgtgtatca ctatcaata gcaatgata attttaata taagctcag gagaaaagt gtccctact tacacaact attccataa atgcgatgg tgaacaaca gaacgaaca tcccgtaca tttatttga acgtaagtt tggatcttg ttgatgcaa acagcgaca aaatcgaga tccatgtga agtcaagac ataggaaca gttgcggat tcatgtatg acaacttcc acgacgccg atcacgaaa ttatcatac cgaaggcat tgccggtta gcgacatta cacccgagt gcgagcttg agatcttga tgtccttcc ccaacgctg attcctgca ccgtctcat acaaagtac cagaaatcc caggtttct gctacgaga tgctcaacc caacttcga aaattttaa gcgtggagc cactttacg acaaccagt ttaaaattc tctgggacc ctggcacca aagccagga acaaccagt cctattacc tgaaaaaag tcaactttt tttacttag ctacggaaa cgccttcga tgcccagtg aagtcgagt ttgttggaa aaccagacc tggaggcac acacagcta cggtggtta tgaagcttt ttgagaatg acaattaca cgctgcagg ggattttca ccaataatt acacctgtt tacaccaag ccgaatttt acttcacag ctcctcctc gccgaagct tacatctgg tgtttttta cccttctga ccagcgttc tagtcgtga tgactttcc tgcataagc gtattttgc ggtatgcga aaaagttgc tagggctgg taatcccaa gatacagga acataactc accgagaga attttgatt tacaaccgt tgcaaagtg ggaaccttg ttcacatga gcatcaaca ctcaataca ctccaatcg aatttttga gaggcgatt tcgacgaat tcgaattcc cccggaaca agataatca ttaaggaaa tcctcggaa ctggagctt ttggccgag tgtattcag ccaaagctc tgggaattg gcggaagtg agggttacc aaatggtgg cggttaaaa cgttagggg aaggagagc aaataacaa gagaggcag ccgatgatt tcaagtcgg aaattgaga tattcaaaa aaattggta agcacccca atattgtgt cgctcctgg gttgttgta ctgtggaag caccctgta tgatgataa tggagttgg tgccgtgtg gcgacctca agaaatatc ttgtggagt tgcgggaac aatggtttg cggaaaaaa ataaacaag tgttttttc ccccagaga acgacaata gtgatgggg catatattg aaccatcga gccctacca gtgttaatt ctataacca caagcaggt taccatcga cttccgaaa ctgtcttta cgaatctgg aggacccca tgacgcccc tcctcgagc ataaccagc aggcccttc agaaggttt tggaccaca aggagctcc agaacttcg ccctgcaaa tcgcgcggg gaatggccc atctggaga agatctcca tcacccacc gcgacctcg ccgctcgca acatcctaa tcaacgagt tcaaaactt tgaaaatct ccgacttcg gactgtccc gaaccgggg tgtacatca accaaagga cgaaaaaac tccccctgc gctggatgg ctctggaag ccatcaccg agcagaagt acgacagta gaagtgacg tttggtctt tcggtgtag tgttgtggg agattggca cacttggtg cgttcccgt acgaaaaag tgcccgatt cgatgattc tgcacttcc tgcagatgg ggcggaggc tggaaaggc cggagatct gcactgatg aactttatt cgctgatga ggcagtgct gggccacgg acccgagtc aaaggccca cgtttagag aactggaga gtgcgctgg atgttaagg cgaagaagg tttacgtga actttgacc aacttaatc cgtcttatg tttttccgc cgagtgatg cgcgtgtgg tgaaagggg tgcccattg ttattaatc cgaggataa ttcaggagg aagaagagg gaacttgat gataataaa attatcgtg atgtgtgat atttcaaaa ttaaacgca ctttgttta tttatttcg taaagttaa ggcacccac tcgtatttg ccatttttc acgcgaaaa aagacgaat ttctgttga aaataaata atttattgt gctttgttt tttctatga aaaaataca tgttttcca ttattaata aattgattt tcatttt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
