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Gene information
General information
TC012303 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 1064301..1068528
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012303
    iBeetle screen iB_01965
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012303 RA
      MARYGQRPE NALKRANEF IEVGKPARA LDTLQEVFR 
      NKKWAYNWS ESVLEPIMF KYLDLCVEL KKSHIAKEG 
      LFQYRNMFQ SVNVGSLEN VIRGYLRMA EEKTENARE 
      QSAQAVIDI DDLDNLATP ESILLSAVS GEDAQDRSD 
      RTILTPWVK FLWESYCQC LELLRTNAH VENLYHDIA 
      RMAFQFCLK YNRKTEFRK LCDKLRKHL EDICKLPAQ 
      VANVSMSKP ETQQLNLET RLHQLDFAI QMELWQEAY 
      KAIEDIHNL MNMSKKMPV PKTMANYYQ KLAMVFWKA 
      GNYLFHAAA LFKLFQLSK EMKKNITQE ELQRMACRV 
      LIATLSIPL PSAHPEFDR FIETDKSPL EKAQRLAVL 
      LGLFQPPTR ASLLKDLVR VNVVSLAST QLQDLYNWL 
      EVDFHPLLL CSRVHTVIQ SLQAEENLL QQYIPALQD 
      VTLVRLVRQ IAQVYQTIE FARLLELAK FTDAFHLER 
      LLVDCVRHN DMQIRIDHG KYCVHFGMD LSESQREDK 
      PEGPTLQVM PSEQVRNQL VNMATVLNQ AINVIYPNK 
      KKAEREKMR SIMVKNYHE TKAQEHEKI LQRHKIIED 
      RKEYIERLN TVREEEEQR RLEELARQQ ALAEQKRLE 
      QEREEREKK RAQNEIQQI KDRHLKEKL QQISQTGHG 
      QKILKKLDE DDIKKLDAD QIAAKEAEE LQKERRELQ 
      AKLKSQEKK VDYFERAKR LEEIPLLQA SIKERQLQD 
      QAFWEQQEK ERIAAAIEE RKLAVATRD RLVRMKPDK 
      DAFLEKLKK ERNIVYEDK LKEFEKLLN EERQKRLLE 
      RKLKRKEER RTRYIKEKQ EEEERKLAE QKRKEEEER 
      QRLEEIARK EREEKERVE REERDRRAR EQQEMLDRT 
      GEKQRKRME EIEKRLAED QDKGREKPK DSWRAKGER 
      EPPKREGGD SSWRRGEKP EEPKKVEAW RPRNMRANP 
      DETKGGDAW RSREAEAEE QPERERREV GRGFERGDR 
      DRFTREDRR GGEREERDR RGDREERDR RGDREERDR 
      RGDREERDR RGDREERDR RGDRDERER RGGDRDMGS 
      WRSVRRTDD EAPRRPPPA RGGGREGGS DWRRREETP 
      RKVEERRAA PPPKEKPRE QQAEEDDGW AEVKRR
    CDS sequence
    • >TC012303 RA
      atggctcga tacggtcaa agacccgaa aatgcccta 
      aaacgggcg aacgaattc atcgaagtc gggaaacca 
      gcgcgtgct ttagacacc ctacaggag gttttccgc 
      aacaaaaaa tgggcctac aactggtcc gagtccgtg 
      ctggagccc atcatgttc aagtacttg gacctgtgc 
      gtggagctg aagaaatcg cacattgcg aaagagggg 
      ctgttccag taccggaac atgttccag tcggtgaac 
      gtgggctcg ctggagaac gtcatcagg gggtacctg 
      cggatggcc gaggagaag acggaaaat gcgcgcgag 
      cagtcggcc caagccgtc atagatatc gacgatttg 
      gacaatttg gccaccccg gagagcatt ttattaagt 
      gctgtctcg ggggaggat gcccaggac cgctccgat 
      cgcacgatt ctgactccg tgggttaaa ttcctgtgg 
      gagtcgtat tgccaatgt ttggagctg ttgaggact 
      aacgctcac gttgaaaat ctctatcat gatattgct 
      aggatggcg ttccagttt tgtctcaaa tataatcga 
      aagaccgaa tttaggaaa ttgtgtgac aagttgagg 
      aagcatctg gaagatatt tgcaaactg ccagctcaa 
      gtggccaat gtttcaatg tctaagcct gaaacgcag 
      caattaaat ctggagact aggctgcat cagctcgat 
      tttgccatc cagatggag ttgtggcag gaagcttac 
      aaagccatt gaggacatc cataaccta atgaacatg 
      tctaaaaaa atgccggtg cctaaaaca atggccaat 
      tactaccaa aaactggcc atggttttc tggaaggcc 
      ggaaattac ctcttccac gcggcagct ctattcaaa 
      ctgttccaa ttatcgaaa gaaatgaag aaaaacatc 
      actcaggag gagctacaa cgaatggct tgtcgcgtt 
      cttatcgcc actctttca atccccctc ccatcggct 
      catcccgaa tttgatcgt tttatcgaa actgacaag 
      tctccactg gaaaaagcg caacgattg gccgtcctt 
      ttgggcctt ttccagcct ccgactcgt gccagtctg 
      ctgaaagat ttagttcgt gttaacgtt gtcagtttg 
      gcttcgact caactccag gatttgtac aattggtta 
      gaagtcgat tttcatcca cttttgctg tgctcgagg 
      gttcatacg gtgattcag tcgttgcaa gctgaagaa 
      aatctgcta cagcagtac ataccagcg ctacaggac 
      gttactctg gtgcgtttg gtgcggcag atcgctcaa 
      gtttaccaa acgattgaa tttgcaagg ttgttggaa 
      ctggcaaag tttactgat gctttccat cttgagcga 
      cttctagtc gattgtgtt agacacaac gatatgcag 
      attaggatc gatcatggc aaatattgc gtccacttt 
      ggtatggat ttgagcgaa agtcagagg gaagataag 
      ccggaagga ccaactctg caagtaatg ccgagtgag 
      caagtgaga aaccaactt gttaatatg gcgactgtg 
      ttaaatcaa gccatcaat gtgatttat ccgaataag 
      aagaaggcc gaaagagag aagatgagg agtattatg 
      gtgaagaat taccacgag actaaggct caagaacat 
      gagaagatc ctccaacgc cataagatt attgaagac 
      aggaaggag tacatcgag agattgaac acagtcagg 
      gaggaagaa gagcaaagg cgtttggag gagttggcc 
      aggcagcag gcgctagcc gagcaaaaa cgcctagaa 
      caagaaagg gaagaaagg gagaagaag agggcccaa 
      aacgagatt caacaaatc aaggacaga cacttgaag 
      gagaaattg cagcaaatt agccaaaca ggtcatggg 
      caaaaaatc ctgaagaag ttggacgag gatgatatt 
      aagaaactc gacgctgac caaatcgcc gctaaagaa 
      gccgaagaa ttacaaaag gaacgtcgc gaacttcaa 
      gccaagctc aaatctcaa gaaaagaaa gtcgattat 
      ttcgaacgt gcgaaacgt ttggaagaa ataccgctg 
      cttcaagcg tccataaaa gaacgtcag ttgcaagac 
      caagccttc tgggagcaa caagaaaaa gaacgcatt 
      gccgcggcc atcgaggag agaaaattg gcagttgcc 
      acacgagac cgtttggtt agaatgaaa cccgacaaa 
      gacgctttc ttggaaaaa ttgaagaaa gaacgtaac 
      atcgtctac gaagataaa ttgaaagag tttgagaag 
      ttgttgaat gaagagcgc cagaaacgc ctgctcgaa 
      cgtaaattg aagcgtaaa gaagaacgc cgtactcgt 
      tacattaaa gaaaaacag gaggaggaa gaacgcaag 
      ttggctgaa caaaaacga aaggaagag gaagaaagg 
      caacgtttg gaggaaatc gcccgaaaa gaacgcgaa 
      gaaaaggaa agagttgaa cgcgaggaa cgagaccgg 
      cgcgctcgc gaacaacag gaaatgtta gacagaaca 
      ggcgaaaaa caacgtaaa cgaatggaa gaaatcgag 
      aaacgccta gcggaagac caagacaaa ggtcgcgaa 
      aaacccaaa gactcctgg cgtgcgaaa ggcgaacgc 
      gagcctccg aaacgcgaa ggcggcgac tcgtcctgg 
      cgccgcggg gaaaaaccc gaggagccg aagaaggtc 
      gaagcttgg cgaccgagg aacatgcgt gcgaatccc 
      gacgaaaca aagggcgga gacgcgtgg aggagccgc 
      gaggcggag gcggaggag cagcccgag cgcgaacgc 
      agggaagtt gggcgtggc ttcgaacgc ggcgatcgc 
      gataggttc acccgagag gaccgccgc ggcggggag 
      agggaagag cgggatagg cgtggcgat agggaggag 
      cgagaccgg cggggtgat cgagaggag cgcgataga 
      cgtggcgat agggaggag agagatagg cgtggcgat 
      cgggaggag cgagacagg cgtggcgat agagatgag 
      cgcgaacgt agaggcggg gatcgagac atggggtcg 
      tggaggagc gtacgtcgg acggacgat gaggcgccg 
      aggaggccc ccaccggcg agaggaggc ggacgggaa 
      ggcgggtca gattggaga cgcagggag gagacaccg 
      aggaaggtg gaggagcga agggcggca ccgcctccg 
      aaggagaaa cctcgtgag caacaagct gaagaggat 
      gatgggtgg gctgaagtc aagcgtcgt taa
    mRNA sequence
    • >TC012303 RA
      gcgccattt tgaaggcgc attgtggaa atttttgac 
      gtttccaca cggctttac cgcttttaa agctttata 
      aatgtgtat taagtgaac caaagtgat aaacaatgg 
      ctcgatacg gtcaaagac ccgaaaatg ccctaaaac 
      gggcgaacg aattcatcg aagtcggga aaccagcgc 
      gtgctttag acaccctac aggaggttt tccgcaaca 
      aaaaatggg cctacaact ggtccgagt ccgtgctgg 
      agcccatca tgttcaagt acttggacc tgtgcgtgg 
      agctgaaga aatcgcaca ttgcgaaag aggggctgt 
      tccagtacc ggaacatgt tccagtcgg tgaacgtgg 
      gctcgctgg agaacgtca tcagggggt acctgcgga 
      tggccgagg agaagacgg aaaatgcgc gcgagcagt 
      cggcccaag ccgtcatag atatcgacg atttggaca 
      atttggcca ccccggaga gcattttat taagtgctg 
      tctcggggg aggatgccc aggaccgct ccgatcgca 
      cgattctga ctccgtggg ttaaattcc tgtgggagt 
      cgtattgcc aatgtttgg agctgttga ggactaacg 
      ctcacgttg aaaatctct atcatgata ttgctagga 
      tggcgttcc agttttgtc tcaaatata atcgaaaga 
      ccgaattta ggaaattgt gtgacaagt tgaggaagc 
      atctggaag atatttgca aactgccag ctcaagtgg 
      ccaatgttt caatgtcta agcctgaaa cgcagcaat 
      taaatctgg agactaggc tgcatcagc tcgattttg 
      ccatccaga tggagttgt ggcaggaag cttacaaag 
      ccattgagg acatccata acctaatga acatgtcta 
      aaaaaatgc cggtgccta aaacaatgg ccaattact 
      accaaaaac tggccatgg ttttctgga aggccggaa 
      attacctct tccacgcgg cagctctat tcaaactgt 
      tccaattat cgaaagaaa tgaagaaaa acatcactc 
      aggaggagc tacaacgaa tggcttgtc gcgttctta 
      tcgccactc tttcaatcc ccctcccat cggctcatc 
      ccgaatttg atcgtttta tcgaaactg acaagtctc 
      cactggaaa aagcgcaac gattggccg tccttttgg 
      gccttttcc agcctccga ctcgtgcca gtctgctga 
      aagatttag ttcgtgtta acgttgtca gtttggctt 
      cgactcaac tccaggatt tgtacaatt ggttagaag 
      tcgattttc atccacttt tgctgtgct cgagggttc 
      atacggtga ttcagtcgt tgcaagctg aagaaaatc 
      tgctacagc agtacatac cagcgctac aggacgtta 
      ctctggtgc gtttggtgc ggcagatcg ctcaagttt 
      accaaacga ttgaatttg caaggttgt tggaactgg 
      caaagttta ctgatgctt tccatcttg agcgacttc 
      tagtcgatt gtgttagac acaacgata tgcagatta 
      ggatcgatc atggcaaat attgcgtcc actttggta 
      tggatttga gcgaaagtc agagggaag ataagccgg 
      aaggaccaa ctctgcaag taatgccga gtgagcaag 
      tgagaaacc aacttgtta atatggcga ctgtgttaa 
      atcaagcca tcaatgtga tttatccga ataagaaga 
      aggccgaaa gagagaaga tgaggagta ttatggtga 
      agaattacc acgagacta aggctcaag aacatgaga 
      agatcctcc aacgccata agattattg aagacagga 
      aggagtaca tcgagagat tgaacacag tcagggagg 
      aagaagagc aaaggcgtt tggaggagt tggccaggc 
      agcaggcgc tagccgagc aaaaacgcc tagaacaag 
      aaagggaag aaagggaga agaagaggg cccaaaacg 
      agattcaac aaatcaagg acagacact tgaaggaga 
      aattgcagc aaattagcc aaacaggtc atgggcaaa 
      aaatcctga agaagttgg acgaggatg atattaaga 
      aactcgacg ctgaccaaa tcgccgcta aagaagccg 
      aagaattac aaaaggaac gtcgcgaac ttcaagcca 
      agctcaaat ctcaagaaa agaaagtcg attatttcg 
      aacgtgcga aacgtttgg aagaaatac cgctgcttc 
      aagcgtcca taaaagaac gtcagttgc aagaccaag 
      ccttctggg agcaacaag aaaaagaac gcattgccg 
      cggccatcg aggagagaa aattggcag ttgccacac 
      gagaccgtt tggttagaa tgaaacccg acaaagacg 
      ctttcttgg aaaaattga agaaagaac gtaacatcg 
      tctacgaag ataaattga aagagtttg agaagttgt 
      tgaatgaag agcgccaga aacgcctgc tcgaacgta 
      aattgaagc gtaaagaag aacgccgta ctcgttaca 
      ttaaagaaa aacaggagg aggaagaac gcaagttgg 
      ctgaacaaa aacgaaagg aagaggaag aaaggcaac 
      gtttggagg aaatcgccc gaaaagaac gcgaagaaa 
      aggaaagag ttgaacgcg aggaacgag accggcgcg 
      ctcgcgaac aacaggaaa tgttagaca gaacaggcg 
      aaaaacaac gtaaacgaa tggaagaaa tcgagaaac 
      gcctagcgg aagaccaag acaaaggtc gcgaaaaac 
      ccaaagact cctggcgtg cgaaaggcg aacgcgagc 
      ctccgaaac gcgaaggcg gcgactcgt cctggcgcc 
      gcggggaaa aacccgagg agccgaaga aggtcgaag 
      cttggcgac cgaggaaca tgcgtgcga atcccgacg 
      aaacaaagg gcggagacg cgtggagga gccgcgagg 
      cggaggcgg aggagcagc ccgagcgcg aacgcaggg 
      aagttgggc gtggcttcg aacgcggcg atcgcgata 
      ggttcaccc gagaggacc gccgcggcg gggagaggg 
      aagagcggg ataggcgtg gcgataggg aggagcgag 
      accggcggg gtgatcgag aggagcgcg atagacgtg 
      gcgataggg aggagagag ataggcgtg gcgatcggg 
      aggagcgag acaggcgtg gcgatagag atgagcgcg 
      aacgtagag gcggggatc gagacatgg ggtcgtgga 
      ggagcgtac gtcggacgg acgatgagg cgccgagga 
      ggcccccac cggcgagag gaggcggac gggaaggcg 
      ggtcagatt ggagacgca gggaggaga caccgagga 
      aggtggagg agcgaaggg cggcaccgc ctccgaagg 
      agaaacctc gtgagcaac aagctgaag aggatgatg 
      ggtgggctg aagtcaagc gtcgttaaa gcgtctatt 
      atggtagaa catcgtcat tattttgtc tctttatat 
      ttaggacaa gattctttt ttaggaagt attttattt 
      ttaaagcta gttgtggga cgctttgta ttatttttt 
      aaatcaata aattttaaa tgaggca