General information | |||
TC012299 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 3887772..3890028 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012299 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01964 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012299 RA
MDLVTQTSL EALLLKATN SQNQNIDTA AVEAFCALV NKEKDGAHI GVKVIANRL PSGNEKELL QTLNILDTC MSKCGTAFQ SEVGKFRFL NEMIKLVSP KYLGSQTPL VVKQKVLQL MYIWTLDYP KETKIKEAY DMLRKQGVI KEIPNPNIV QNANIPRKK AANSVFQDE EKSKILQKL LQSKDPEDI QAANWLIKS MVKEDDKRA ELKSRRVSE LESVQNNVR LLNEMLDSY KPGISSADE LDLIKELHQ SCERLRPNL MKLTAETQQ NEEILGDVL QASDELSQV FGKYNRIIV QGVSVVDDG SNALLDFEG ADEAQCRNS DTKEIDVLC DIFNASNIP DSADILKPV SVLTCDKNE NGKEVNKFK ALEELDVLS EHFIKENLP TSRQGTFNN RQDKIPMNL LTKLSETKP VQSNGKNES ISESLSLDL SYLISKPKD EPKTNTMEN STDDCLVDI ADEKMLEAG ETPQRKPSL TDPAKNEIK LNDIFIKLE DIKPSPVPP LTILNERNG ITITLHFVK DQPRDDVHV FVVTTINKN EMPLTNYLF QAVVPKGCK VKLQPPSAT TLPAYNPFL PPSAITQIM LIANPDDVQ VFLKFFVSF VMDEETCTE IGEVETLPM
- >TC012299 RA
atggatttg gtgactcaa actagtctc gaagccctg cttttaaaa gccacaaac tcccaaaac caaaacatt gacaccgcc gctgttgag gccttctgc gcccttgta aacaaagag aaagatggg gcacacatc ggggttaaa gtgatagca aaccggctt ccttcaggt aacgagaaa gagctgctc caaacgctc aacatcctg gatacgtgc atgagcaaa tgtggcaca gctttccag tcagaagtc ggcaaattc cggtttctg aacgaaatg attaagcta gtttcgccc aaatatctg gggtcgcag acgccgctc gtggtgaag caaaaagtg ctgcaactg atgtacatt tggacactg gactacccc aaagaaacg aagataaag gaagcgtac gatatgctg cgaaagcag ggcgttatc aaggaaatc ccgaacccc aacatagta caaaacgcc aacattccg aggaaaaag gccgcgaat tccgtgttc caagacgag gagaaatca aaaattttg caaaagctg ctgcaaagt aaagacccc gaggacatc caagccgcc aattggctc attaaaagt atggtcaag gaggacgat aaaagggcg gaattgaag agccggcgc gtttccgag cttgagtca gtacaaaac aatgtgaga ttattgaat gaaatgctc gattcgtac aaaccgggc atttcttcc gccgatgag ctggacctt attaaagag ctgcatcaa agttgtgag cgtctgaga ccaaattta atgaaacta accgcagag acacaacaa aatgaagaa atcttaggt gatgttttg caagcaagc gatgagttg agccaagtt tttggcaag tataacagg attattgtg cagggagtt agtgtcgtg gacgatggc agcaatgcc ttgcttgat tttgagggc gcagacgag gctcagtgt cggaactct gataccaaa gaaattgac gttttatgt gatattttc aacgcttca aacattccg gactctgct gatattttg aaacctgtt tcagtatta acttgtgat aaaaacgaa aatgggaaa gaggtgaat aaattcaaa gccttggaa gagttggac gttttgagc gaacacttt attaaggaa aatctccca actagcaga caaggcact tttaataat aggcaagac aaaatccca atgaatctc cttacaaag ctatctgag acgaaacca gttcagagt aacggaaag aacgaatcg attagtgaa tctctaagt ctagatttg agttattta ataagcaaa ccaaaagac gaaccaaaa acaaacaca atggaaaat tccactgat gactgtttg gtggacata gctgacgag aaaatgctg gaagctggg gaaaccccc caacgaaag cccagctta actgacccg gcaaaaaat gaaataaaa ctgaacgac atttttatc aaattggaa gatatcaag ccaagtccc gtcccacct ctcacgatt ttaaacgag aggaacgga atcacaata accctgcat ttcgtgaaa gaccagccc cgagacgac gtgcatgtc tttgttgtg accacaatt aacaaaaac gagatgcct ttgactaac tacctgttc caggctgtg gtacctaag ggctgcaaa gtcaaactg caacctccc tcagcaacc accctcccg gcttacaac ccgttcctg cccccttct gccataacg caaatcatg ctgatcgca aatcctgat gatgtacaa gtttttctc aagtttttt gtaagtttt gtaatggac gaagagact tgtactgaa atcggcgag gttgaaact cttccaatg taa
- >TC012299 RA
tgacgttta agtgacatg aaaataaaa tgattgaaa caatttaaa aacccataa aatggattt ggtgactca aactagtct cgaagccct gcttttaaa agccacaaa ctcccaaaa ccaaaacat tgacaccgc cgctgttga ggccttctg cgcccttgt aaacaaaga gaaagatgg ggcacacat cggggttaa agtgatagc aaaccggct tccttcagg taacgagaa agagctgct ccaaacgct caacatcct ggatacgtg catgagcaa atgtggcac agctttcca gtcagaagt cggcaaatt ccggtttct gaacgaaat gattaagct agtttcgcc caaatatct ggggtcgca gacgccgct cgtggtgaa gcaaaaagt gctgcaact gatgtacat ttggacact ggactaccc caaagaaac gaagataaa ggaagcgta cgatatgct gcgaaagca gggcgttat caaggaaat cccgaaccc caacatagt acaaaacgc caacattcc gaggaaaaa ggccgcgaa ttccgtgtt ccaagacga ggagaaatc aaaaatttt gcaaaagct gctgcaaag taaagaccc cgaggacat ccaagccgc caattggct cattaaaag tatggtcaa ggaggacga taaaagggc ggaattgaa gagccggcg cgtttccga gcttgagtc agtacaaaa caatgtgag attattgaa tgaaatgct cgattcgta caaaccggg catttcttc cgccgatga gctggacct tattaaaga gctgcatca aagttgtga gcgtctgag accaaattt aatgaaact aaccgcaga gacacaaca aaatgaaga aatcttagg tgatgtttt gcaagcaag cgatgagtt gagccaagt ttttggcaa gtataacag gattattgt gcagggagt tagtgtcgt ggacgatgg cagcaatgc cttgcttga ttttgaggg cgcagacga ggctcagtg tcggaactc tgataccaa agaaattga cgttttatg tgatatttt caacgcttc aaacattcc ggactctgc tgatatttt gaaacctgt ttcagtatt aacttgtga taaaaacga aaatgggaa agaggtgaa taaattcaa agccttgga agagttgga cgttttgag cgaacactt tattaagga aaatctccc aactagcag acaaggcac ttttaataa taggcaaga caaaatccc aatgaatct ccttacaaa gctatctga gacgaaacc agttcagag taacggaaa gaacgaatc gattagtga atctctaag tctagattt gagttattt aataagcaa accaaaaga cgaaccaaa aacaaacac aatggaaaa ttccactga tgactgttt ggtggacat agctgacga gaaaatgct ggaagctgg ggaaacccc ccaacgaaa gcccagctt aactgaccc ggcaaaaaa tgaaataaa actgaacga catttttat caaattgga agatatcaa gccaagtcc cgtcccacc tctcacgat tttaaacga gaggaacgg aatcacaat aaccctgca tttcgtgaa agaccagcc ccgagacga cgtgcatgt ctttgttgt gaccacaat taacaaaaa cgagatgcc tttgactaa ctacctgtt ccaggctgt ggtacctaa gggctgcaa agtcaaact gcaacctcc ctcagcaac caccctccc ggcttacaa cccgttcct gcccccttc tgccataac gcaaatcat gctgatcgc aaatcctga tgatgtaca agtttttct caagttttt tgtaagttt tgtaatgga cgaagagac ttgtactga aatcggcga ggttgaaac tcttccaat gtaaagcat tttaatttt agtgtaatt aaatcgtgc ttaagtca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
