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Gene information
General information
TC012298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3886202..3887474
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012298
    iBeetle screen iB_01963

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012298 RA
      MVEEHEKKC EFVAIHECS ECDYRGNGC QLYHHFLKK 
      HPDNFLKYP EFSVDLLEK KNWNYLYRV GNHLFFIAY 
      QILPHHRVL KLSMNCIGR NKETTKCQI ILKSLTSNK 
      EYFRSSEEN FLEEKIDIL EHLEKNKEN VVACLLKVK 
      RGAELFTYR SNKPERQVK QIVKALNKN LFPKLLCPC 
      GSFVLPPIL KCPSNHVQC ESCATSYCP LCSDVVNWS 
      RAPDLEAFH DIIPLPCRW QCETLLLHP ELRSHEKTC 
      SKRLYKCIE KWCSWSGSL NELMRHWHS SEPVYDRAH 
      QVYGFSEGL VEFYLVYNN ELFYLRMDS LKNSGRNFH 
      VRKVSNVET RNEAYAASV VLRHSCDGV TMEKNAVLK 
      FGGITRLGK SYFSHFGEC KNYKIIFNL QLT
    CDS sequence
    • >TC012298 RA
      atggtggaa gaacacgaa aagaaatgt gaatttgtt 
      gccatccac gagtgtagc gagtgtgat tacagggga 
      aacggttgt caactctat caccacttc cttaaaaaa 
      catcctgac aactttcta aaatacccc gagtttagt 
      gtcgattta ctcgaaaag aaaaactgg aattactta 
      tatcgggtc ggcaaccac ttatttttt attgcctac 
      caaattctt ccacatcat agagttttg aaattgtcc 
      atgaattgc attggacga aacaaagaa accaccaaa 
      tgccaaatt attctaaaa tcactgacc tcgaataaa 
      gaatacttt cgaagcagt gaagaaaat tttcttgag 
      gaaaaaatc gatatttta gaacatttg gaaaagaac 
      aaagaaaac gtggtggcg tgtctgttg aaggtaaaa 
      cggggtgca gaattattc acttacaga agtaacaag 
      ccagaacgt caagttaaa caaatagtg aaagccctc 
      aacaaaaat ctgttcccc aaactgctt tgcccctgt 
      ggtagtttc gtgctgccc ccgattctg aaatgcccc 
      tccaaccac gtgcagtgc gagtcttgc gccacctcc 
      tattgcccc ctttgcagc gacgtggta aattggtcg 
      cgagccccc gatttggag gccttccat gacatcatc 
      cccctcccc tgtcgctgg cagtgcgaa accctgtta 
      ctccacccg gaattgcgt tcgcacgaa aaaacttgt 
      agcaaaaga ctttacaag tgtatcgaa aagtggtgt 
      tcatggagt ggaagttta aacgagctt atgagacat 
      tggcacagc tccgagccg gtatatgac cgtgctcac 
      caagtgtac ggcttcagc gaaggcctc gttgaattc 
      tatttggta tacaacaat gaactgttt tatttaaga 
      atggattcg ttgaagaat tcaggcaga aattttcat 
      gtaaggaaa gtttcgaat gtggagacg agaaatgag 
      gcatacgcg gcgagcgtg gtgctgagg catagctgc 
      gatggggtg acaatggag aaaaatgcc gtccttaag 
      tttggggga attacccgg ctgggaaag agctatttc 
      agccacttt ggggagtgc aaaaattat aaaattatc 
      tttaacctg cagctgaca taa
    mRNA sequence
    • >TC012298 RA
      atagttgtg atgaattaa tctgttaca caatggtgg 
      aagaacacg aaaagaaat gtgaatttg ttgccatcc 
      acgagtgta gcgagtgtg attacaggg gaaacggtt 
      gtcaactct atcaccact tccttaaaa aacatcctg 
      acaactttc taaaatacc ccgagttta gtgtcgatt 
      tactcgaaa agaaaaact ggaattact tatatcggg 
      tcggcaacc acttatttt ttattgcct accaaattc 
      ttccacatc atagagttt tgaaattgt ccatgaatt 
      gcattggac gaaacaaag aaaccacca aatgccaaa 
      ttattctaa aatcactga cctcgaata aagaatact 
      ttcgaagca gtgaagaaa attttcttg aggaaaaaa 
      tcgatattt tagaacatt tggaaaaga acaaagaaa 
      acgtggtgg cgtgtctgt tgaaggtaa aacggggtg 
      cagaattat tcacttaca gaagtaaca agccagaac 
      gtcaagtta aacaaatag tgaaagccc tcaacaaaa 
      atctgttcc ccaaactgc tttgcccct gtggtagtt 
      tcgtgctgc ccccgattc tgaaatgcc cctccaacc 
      acgtgcagt gcgagtctt gcgccacct cctattgcc 
      ccctttgca gcgacgtgg taaattggt cgcgagccc 
      ccgatttgg aggccttcc atgacatca tccccctcc 
      cctgtcgct ggcagtgcg aaaccctgt tactccacc 
      cggaattgc gttcgcacg aaaaaactt gtagcaaaa 
      gactttaca agtgtatcg aaaagtggt gttcatgga 
      gtggaagtt taaacgagc ttatgagac attggcaca 
      gctccgagc cggtatatg accgtgctc accaagtgt 
      acggcttca gcgaaggcc tcgttgaat tctatttgg 
      tatacaaca atgaactgt tttatttaa gaatggatt 
      cgttgaaga attcaggca gaaattttc atgtaagga 
      aagtttcga atgtggaga cgagaaatg aggcatacg 
      cggcgagcg tggtgctga ggcatagct gcgatgggg 
      tgacaatgg agaaaaatg ccgtcctta agtttgggg 
      gaattaccc ggctgggaa agagctatt tcagccact 
      ttggggagt gcaaaaatt ataaaatta tctttaacc 
      tgcagctga cataacggg gggatttac ccgatttgt 
      cgcttatca tttttaatc gctaagtag ataatacaa 
      cttgtgaat taat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY