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Gene information
General information
TC012290 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 937177..938778
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012290
    iBeetle screen iB_01962
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012290 RA
      MFTGITLQI ISALIATLS AFVDGMHFA WTAPIVPVL 
      RRPESPIKI TPNDVTLLE TIYLSGGVV GLPITIFLV 
      NKIGRKKSI LTASAINLI AWIIIGTAD QVQYLYLAR 
      FLGGLEANV NYVSTPMYL AEIAEEKFR GVLSGSLYV 
      MVTFGMLTI YSVAPFLPF YVPSVVAGV LLVTQLVTF 
      GILPESPYY LMKQGQEEK AKAALLKLR GNLDKLETI 
      SSAVRQQQR ERGRLRELF SVSNNRRAF TIVLYLTAA 
      QHFSGITPI LMNLHTILV DTDSTFFNY NMTAILFVA 
      VMLITSIVT SFFLDKLGR KFLLIISSI VCGSCLLIM 
      AVYFHLKTL GKVDSSAFG WIPLVAVMT YAAGFRSGI 
      GLVPIVLAS ELFSMNVKA LGMSLSDGM YVTFGFICI 
      EIYQSIVHY CGYYVPFYI FTIVAFVTA VFAFFVIPE 
      TKGKSLEEI QILLKKRKP THTNDKYEN CDLVIA
    CDS sequence
    • >TC012290 RA
      atgttcacc ggaatcaca ttacaaata atctcggca 
      cttatagcc acactgagt gcatttgtt gatggtatg 
      cattttgcg tggactgca ccaatagtt cccgtccta 
      cgtagaccc gaaagtccg ataaaaata accccaaat 
      gacgtgacg ctcctggaa accatctac ctctccgga 
      ggagttgtg ggtctgcct ataacaatt ttcctagtc 
      aataaaatc gggcgaaaa aagtcgatt ttaacagcc 
      tcggctatc aacctaatc gcttggata attatagga 
      accgccgac caggtgcag tatttatac ttagcacgg 
      tttttgggc ggattagaa gcgaacgtc aactacgtc 
      tcaactccg atgtactta gccgaaatc gccgaagaa 
      aaattcagg ggggttctg tcggggagt ttgtacgtg 
      atggtgact ttcggcatg ctcaccatc tactcagtg 
      gcccccttc ctgcccttc tatgtcccg tcggtggta 
      gccggagtg ctgctggtg acccagctg gtcacattt 
      gggattttg ccggaatcg ccctactac ctgatgaag 
      cagggacag gaggaaaag gccaaagcg gctctgctg 
      aagctgcga ggaaacctc gacaagctc gaaaccata 
      tcttcagcc gttcgacaa caacaaaga gagagggga 
      cgcctccgt gagcttttc tcggtgtca aacaaccgg 
      agagctttc acaattgtg ttgtatctg acggcagcg 
      caacatttc tccgggata accccgatt ttgatgaac 
      ttgcataca atcctggtt gatacggac tcaactttt 
      ttcaattat aacatgact gccattttg ttcgtcgct 
      gtgatgcta attacttcg attgtaact tcgtttttc 
      cttgacaaa ctcgggcgg aaatttctt ttaattatc 
      tcgagtata gtttgtggt tcgtgtttg ctaattatg 
      gccgtttat tttcattta aaaacgctg ggaaaggtc 
      gattcgagt gcatttggt tggattcct ctggttgca 
      gtaatgaca tatgctgca ggatttcgc agtgggatc 
      ggtttagtg ccgatagtt ctagcctcg gaactattt 
      tcaatgaat gttaaagct ctcggcatg tctttgagt 
      gacggaatg tacgttact ttcggattt atttgtata 
      gaaatttat cagagtatt gttcattat tgcgggtat 
      tatgttcct ttttatatt tttacaata gtcgcattt 
      gtgacagca gtttttgct ttctttgtc attccagaa 
      actaaaggt aaatcgtta gaagaaata cagatactg 
      ttgaaaaaa cgaaaaccc acacacacg aacgacaaa 
      tacgaaaac tgtgatctt gtgattgct tag
    mRNA sequence
    • >TC012290 RA
      gcaaaaatg agacaccac aacagtcaa attgtgcct 
      tttgtgaaa caacatcat gttcaccgg aatcacatt 
      acaaataat ctcggcact tatagccac actgagtgc 
      atttgttga tggtatgca ttttgcgtg gactgcacc 
      aatagttcc cgtcctacg tagacccga aagtccgat 
      aaaaataac cccaaatga cgtgacgct cctggaaac 
      catctacct ctccggagg agttgtggg tctgcctat 
      aacaatttt cctagtcaa taaaatcgg gcgaaaaaa 
      gtcgatttt aacagcctc ggctatcaa cctaatcgc 
      ttggataat tataggaac cgccgacca ggtgcagta 
      tttatactt agcacggtt tttgggcgg attagaagc 
      gaacgtcaa ctacgtctc aactccgat gtacttagc 
      cgaaatcgc cgaagaaaa attcagggg ggttctgtc 
      ggggagttt gtacgtgat ggtgacttt cggcatgct 
      caccatcta ctcagtggc ccccttcct gcccttcta 
      tgtcccgtc ggtggtagc cggagtgct gctggtgac 
      ccagctggt cacatttgg gattttgcc ggaatcgcc 
      ctactacct gatgaagca gggacagga ggaaaaggc 
      caaagcggc tctgctgaa gctgcgagg aaacctcga 
      caagctcga aaccatatc ttcagccgt tcgacaaca 
      acaaagaga gaggggacg cctccgtga gcttttctc 
      ggtgtcaaa caaccggag agctttcac aattgtgtt 
      gtatctgac ggcagcgca acatttctc cgggataac 
      cccgatttt gatgaactt gcatacaat cctggttga 
      tacggactc aactttttt caattataa catgactgc 
      cattttgtt cgtcgctgt gatgctaat tacttcgat 
      tgtaacttc gtttttcct tgacaaact cgggcggaa 
      atttctttt aattatctc gagtatagt ttgtggttc 
      gtgtttgct aattatggc cgtttattt tcatttaaa 
      aacgctggg aaaggtcga ttcgagtgc atttggttg 
      gattcctct ggttgcagt aatgacata tgctgcagg 
      atttcgcag tgggatcgg tttagtgcc gatagttct 
      agcctcgga actattttc aatgaatgt taaagctct 
      cggcatgtc tttgagtga cggaatgta cgttacttt 
      cggatttat ttgtataga aatttatca gagtattgt 
      tcattattg cgggtatta tgttccttt ttatatttt 
      tacaatagt cgcatttgt gacagcagt ttttgcttt 
      ctttgtcat tccagaaac taaaggtaa atcgttaga 
      agaaataca gatactgtt gaaaaaacg aaaacccac 
      acacacgaa cgacaaata cgaaaactg tgatcttgt 
      gattgctta gatgtgtat tttatttat ttttgttac 
      aagctatga caagaatat tgatttgta tgcgcttac 
      tgttattta attcaaaat aaataaatt ttagtacaa 
      act