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Gene information
General information
TC012280 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3745989..3751413
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012280
    iBeetle screen iB_01960

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    tropharium size decreased - Number of animals showing the phenotype: 4

    egg chamber number decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber potentially position irregular - Number of animals showing the phenotype: 4

    ovariole sometimes duplicated - Number of animals showing the phenotype: 4

    vitellogenic egg chamber sometimes differentiation irregular - Number of animals showing the phenotype: 4

    Comment: some of the egg chambers seem polynucleated
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012280 RA
      MNAINKNRD RILEAIDQL RRRKARPDI QRICNYLFR 
      RFSISSVEA RSDLQWCVE NDIVLKVEY KGSISYRNA 
      AKKFSHVKR DSQSEHNAD NKANRKFAQ LLTNAFGEL 
      VVHEPDYLE YGVPAEEII DNILSKDSV RYTRKYISI 
      LIEKEVENG GLIKMEHDR YLMGPTKER TDDKGASGE 
      AKDGDTHSQ DLVISVKPR KKPGPKPGL KKEKSESMD 
      SDEKKSETG SLRVGGRRK RAKKVFDPS DTHVPKKRG 
      RPAGSLNKS TIEKQLAKL SDESRPESR TSNSGREQG 
      GVCSVCHVQ NKMGPNDRM VACRECSNK AHYSCLNGD 
      DMMLRMYPD NTWQCPHCK TCVICFETS DAGYLTVCA 
      VCADAYHAG CHQPRIHEK FIKPPAKWL CINCEMPEE 
      LKINEIQSN ISSSFTSKL TNGNDTNDN NSSSPSISG 
      NTTPNYQSP PLSPTPPQL SPQNEAPQN GDGGVSDSQ 
      SNQSVKDSD DDNIDPSIP DATNWTIEE VYQYFAQYF 
      PEEAKIFKE QEIDGRSLL LLKRMDVLT NLKLKLGPA 
      LKIYKHVVK LQVRRDDPK FYWL
    • >TC012280 RB
      MNAINKNRD RILEAIDQL RRRKARPDI QRICNYLFR 
      RFSISSVEA RSDLQWCVE NDIVLKVEY KGSISYRNA 
      AKKFSHVKR DSQSEHNAD NKANRKFAQ LLTNAFGEL 
      VVHEPDYLE YGVPAEEII DNILSKDSV RYTRKYISI 
      LIEKEVENG GLIKMEHDR YLMGPTKER TDDKGASGE 
      AKDGDTHSQ DLVISVKPR KKPGPKPGL KKEKSESMD 
      SDEKKSETG SLRVGGRRK TNKPKMRAK KVFDPSDTH 
      VPKKRGRPA GSLNKSTIE KQLAKLSDE SRPESRTSN 
      SGREQGGVC SVCHVQNKM GPNDRMVAC RECSNKAHY 
      SCLNGDDMM LRMYPDNTW QCPHCKTCV ICFETSDAG 
      YLTVCAVCA DAYHAGCHQ PRIHEKFIK PPAKWLCIN 
      CEMPEELKI NEIQSNISS SFTSKLTNG NDTNDNNSS 
      SPSISGNTT PNYQSPPLS PTPPQLSPQ NEAPQNGDG 
      GVSDSQSNQ SVKDSDDDN IDPSIPDAT NWTIEEVYQ 
      YFAQYFPEE AKIFKEQEI DGRSLLLLK RMDVLTNLK 
      LKLGPALKI YKHVVKLQV RRDDPKFYW L
    CDS sequence
    • >TC012280 RA
      atgaacgcg attaacaag aaccgagac cgcattctg 
      gaggcgatt gaccagctc aggcgccgc aaagcgcgc 
      cccgacatc cagcgcatt tgcaattac ctcttccgg 
      cggttttcc ataagttcg gtggaggcc cgctccgac 
      ctgcaatgg tgcgtggag aacgacatc gtcctcaaa 
      gtggagtac aaagggagc atcagttac aggaacgcg 
      gcgaaaaag ttctcgcac gtgaaacgc gacagccag 
      agcgagcac aacgccgac aacaaagcc aaccggaag 
      ttcgcacaa ctcctcacg aacgccttc ggggagctg 
      gtggtgcac gagcccgac tacctggag tacggcgtg 
      ccggcggag gagatcatc gacaacatc ctctccaag 
      gacagcgtg cgctacacc aggaagtac atctcgatc 
      ctgatcgag aaggaggtg gagaacggc ggcctcatc 
      aagatggaa catgacagg tacctgatg ggaccgacc 
      aaagagagg acggacgat aagggcgcc agcggggaa 
      gcaaaggac ggcgatacg cactcgcag gacctagtc 
      attagcgtc aaaccccgg aaaaaaccg ggacccaaa 
      cccggactc aaaaaggaa aagtccgaa agcatggat 
      tcggacgag aaaaaatcc gaaacgggc agtctgcga 
      gtaggggga aggcgaaag agggccaag aaagtgttc 
      gacccgtcc gacacccac gtcccgaag aagcgcggc 
      cgtccagct gggtccctc aataaatcg acaatcgag 
      aagcaatta gcgaaattg agtgatgag tccaggcct 
      gaatcgcgg acttcgaac agtggtcgg gaacagggt 
      ggtgtttgt tctgtttgt catgtccag aacaaaatg 
      gggcccaat gatcgcatg gtggcctgt cgggaatgt 
      agtaataaa gctcattac agttgcttg aatggggac 
      gatatgatg ctgaggatg tatccagat aatacgtgg 
      caatgtccg cattgtaaa acttgtgtt atttgtttt 
      gagacatcg gatgcgggt tatttaaca gtttgcgct 
      gtctgtgct gatgcttac cacgcagga tgtcaccaa 
      ccgcgcatt catgagaaa tttattaaa ccaccggcg 
      aaatggctt tgcatcaat tgcgaaatg cccgaggaa 
      ctgaaaatc aatgaaatc cagtccaac ataagcagt 
      tctttcacg tctaaatta accaacggg aatgacaca 
      aacgataac aattcgtca tcaccttcc atttctgga 
      aatacaacc cctaattac caatcgccg cccttatca 
      cccacgccg cctcagctc agtccccaa aacgaagcg 
      ccacaaaac ggcgatgga ggggtttct gattctcag 
      agtaaccaa agtgttaaa gattccgat gatgataac 
      atagatccg tctatacct gatgcgacc aattggaca 
      atcgaagag gtgtatcag tatttcgct cagtatttc 
      cccgaggaa gccaaaatt tttaaggaa caggaaatt 
      gatggccgt tcgttgttg ttgctgaaa cgaatggac 
      gttttgaca aatttgaaa ctaaaactg ggacctgca 
      ctcaaaatc tacaagcat gttgttaag ctacaagtg 
      agaagggat gatccaaag ttctactgg ttgtaa
    • >TC012280 RB
      atgaacgcg attaacaag aaccgagac cgcattctg 
      gaggcgatt gaccagctc aggcgccgc aaagcgcgc 
      cccgacatc cagcgcatt tgcaattac ctcttccgg 
      cggttttcc ataagttcg gtggaggcc cgctccgac 
      ctgcaatgg tgcgtggag aacgacatc gtcctcaaa 
      gtggagtac aaagggagc atcagttac aggaacgcg 
      gcgaaaaag ttctcgcac gtgaaacgc gacagccag 
      agcgagcac aacgccgac aacaaagcc aaccggaag 
      ttcgcacaa ctcctcacg aacgccttc ggggagctg 
      gtggtgcac gagcccgac tacctggag tacggcgtg 
      ccggcggag gagatcatc gacaacatc ctctccaag 
      gacagcgtg cgctacacc aggaagtac atctcgatc 
      ctgatcgag aaggaggtg gagaacggc ggcctcatc 
      aagatggaa catgacagg tacctgatg ggaccgacc 
      aaagagagg acggacgat aagggcgcc agcggggaa 
      gcaaaggac ggcgatacg cactcgcag gacctagtc 
      attagcgtc aaaccccgg aaaaaaccg ggacccaaa 
      cccggactc aaaaaggaa aagtccgaa agcatggat 
      tcggacgag aaaaaatcc gaaacgggc agtctgcga 
      gtaggggga aggcgaaag accaacaaa cccaaaatg 
      agggccaag aaagtgttc gacccgtcc gacacccac 
      gtcccgaag aagcgcggc cgtccagct gggtccctc 
      aataaatcg acaatcgag aagcaatta gcgaaattg 
      agtgatgag tccaggcct gaatcgcgg acttcgaac 
      agtggtcgg gaacagggt ggtgtttgt tctgtttgt 
      catgtccag aacaaaatg gggcccaat gatcgcatg 
      gtggcctgt cgggaatgt agtaataaa gctcattac 
      agttgcttg aatggggac gatatgatg ctgaggatg 
      tatccagat aatacgtgg caatgtccg cattgtaaa 
      acttgtgtt atttgtttt gagacatcg gatgcgggt 
      tatttaaca gtttgcgct gtctgtgct gatgcttac 
      cacgcagga tgtcaccaa ccgcgcatt catgagaaa 
      tttattaaa ccaccggcg aaatggctt tgcatcaat 
      tgcgaaatg cccgaggaa ctgaaaatc aatgaaatc 
      cagtccaac ataagcagt tctttcacg tctaaatta 
      accaacggg aatgacaca aacgataac aattcgtca 
      tcaccttcc atttctgga aatacaacc cctaattac 
      caatcgccg cccttatca cccacgccg cctcagctc 
      agtccccaa aacgaagcg ccacaaaac ggcgatgga 
      ggggtttct gattctcag agtaaccaa agtgttaaa 
      gattccgat gatgataac atagatccg tctatacct 
      gatgcgacc aattggaca atcgaagag gtgtatcag 
      tatttcgct cagtatttc cccgaggaa gccaaaatt 
      tttaaggaa caggaaatt gatggccgt tcgttgttg 
      ttgctgaaa cgaatggac gttttgaca aatttgaaa 
      ctaaaactg ggacctgca ctcaaaatc tacaagcat 
      gttgttaag ctacaagtg agaagggat gatccaaag 
      ttctactgg ttgtaa
    mRNA sequence
    • >TC012280 RA
      tgcgtggta tgaaccgct cggtttccg caagaaata 
      ggcgacgga cgggccgcc aacccgccc caaataatc 
      gcaaacagt taggggaag cgatcggtg ccgggccca 
      gcgcccgca agggccgcg ttctcgcgc ttttccgtg 
      tgcgtttcc cggtgcgaa atgaacgcg attaacaag 
      aaccgagac cgcattctg gaggcgatt gaccagctc 
      aggcgccgc aaagcgcgc cccgacatc cagcgcatt 
      tgcaattac ctcttccgg cggttttcc ataagttcg 
      gtggaggcc cgctccgac ctgcaatgg tgcgtggag 
      aacgacatc gtcctcaaa gtggagtac aaagggagc 
      atcagttac aggaacgcg gcgaaaaag ttctcgcac 
      gtgaaacgc gacagccag agcgagcac aacgccgac 
      aacaaagcc aaccggaag ttcgcacaa ctcctcacg 
      aacgccttc ggggagctg gtggtgcac gagcccgac 
      tacctggag tacggcgtg ccggcggag gagatcatc 
      gacaacatc ctctccaag gacagcgtg cgctacacc 
      aggaagtac atctcgatc ctgatcgag aaggaggtg 
      gagaacggc ggcctcatc aagatggaa catgacagg 
      tacctgatg ggaccgacc aaagagagg acggacgat 
      aagggcgcc agcggggaa gcaaaggac ggcgatacg 
      cactcgcag gacctagtc attagcgtc aaaccccgg 
      aaaaaaccg ggacccaaa cccggactc aaaaaggaa 
      aagtccgaa agcatggat tcggacgag aaaaaatcc 
      gaaacgggc agtctgcga gtaggggga aggcgaaag 
      agggccaag aaagtgttc gacccgtcc gacacccac 
      gtcccgaag aagcgcggc cgtccagct gggtccctc 
      aataaatcg acaatcgag aagcaatta gcgaaattg 
      agtgatgag tccaggcct gaatcgcgg acttcgaac 
      agtggtcgg gaacagggt ggtgtttgt tctgtttgt 
      catgtccag aacaaaatg gggcccaat gatcgcatg 
      gtggcctgt cgggaatgt agtaataaa gctcattac 
      agttgcttg aatggggac gatatgatg ctgaggatg 
      tatccagat aatacgtgg caatgtccg cattgtaaa 
      acttgtgtt atttgtttt gagacatcg gatgcgggt 
      tatttaaca gtttgcgct gtctgtgct gatgcttac 
      cacgcagga tgtcaccaa ccgcgcatt catgagaaa 
      tttattaaa ccaccggcg aaatggctt tgcatcaat 
      tgcgaaatg cccgaggaa ctgaaaatc aatgaaatc 
      cagtccaac ataagcagt tctttcacg tctaaatta 
      accaacggg aatgacaca aacgataac aattcgtca 
      tcaccttcc atttctgga aatacaacc cctaattac 
      caatcgccg cccttatca cccacgccg cctcagctc 
      agtccccaa aacgaagcg ccacaaaac ggcgatgga 
      ggggtttct gattctcag agtaaccaa agtgttaaa 
      gattccgat gatgataac atagatccg tctatacct 
      gatgcgacc aattggaca atcgaagag gtgtatcag 
      tatttcgct cagtatttc cccgaggaa gccaaaatt 
      tttaaggaa caggaaatt gatggccgt tcgttgttg 
      ttgctgaaa cgaatggac gttttgaca aatttgaaa 
      ctaaaactg ggacctgca ctcaaaatc tacaagcat 
      gttgttaag ctacaagtg agaagggat gatccaaag 
      ttctactgg ttgtaaata ttgctttta acagttact 
      ttg
    • >TC012280 RB
      tgcgtggta tgaaccgct cggtttccg caagaaata 
      ggcgacgga cgggccgcc aacccgccc caaataatc 
      gcaaacagt taggggaag cgatcggtg ccgggccca 
      gcgcccgca agggccgcg ttctcgcgc ttttccgtg 
      tgcgtttcc cggtgcgaa atgaacgcg attaacaag 
      aaccgagac cgcattctg gaggcgatt gaccagctc 
      aggcgccgc aaagcgcgc cccgacatc cagcgcatt 
      tgcaattac ctcttccgg cggttttcc ataagttcg 
      gtggaggcc cgctccgac ctgcaatgg tgcgtggag 
      aacgacatc gtcctcaaa gtggagtac aaagggagc 
      atcagttac aggaacgcg gcgaaaaag ttctcgcac 
      gtgaaacgc gacagccag agcgagcac aacgccgac 
      aacaaagcc aaccggaag ttcgcacaa ctcctcacg 
      aacgccttc ggggagctg gtggtgcac gagcccgac 
      tacctggag tacggcgtg ccggcggag gagatcatc 
      gacaacatc ctctccaag gacagcgtg cgctacacc 
      aggaagtac atctcgatc ctgatcgag aaggaggtg 
      gagaacggc ggcctcatc aagatggaa catgacagg 
      tacctgatg ggaccgacc aaagagagg acggacgat 
      aagggcgcc agcggggaa gcaaaggac ggcgatacg 
      cactcgcag gacctagtc attagcgtc aaaccccgg 
      aaaaaaccg ggacccaaa cccggactc aaaaaggaa 
      aagtccgaa agcatggat tcggacgag aaaaaatcc 
      gaaacgggc agtctgcga gtaggggga aggcgaaag 
      accaacaaa cccaaaatg agggccaag aaagtgttc 
      gacccgtcc gacacccac gtcccgaag aagcgcggc 
      cgtccagct gggtccctc aataaatcg acaatcgag 
      aagcaatta gcgaaattg agtgatgag tccaggcct 
      gaatcgcgg acttcgaac agtggtcgg gaacagggt 
      ggtgtttgt tctgtttgt catgtccag aacaaaatg 
      gggcccaat gatcgcatg gtggcctgt cgggaatgt 
      agtaataaa gctcattac agttgcttg aatggggac 
      gatatgatg ctgaggatg tatccagat aatacgtgg 
      caatgtccg cattgtaaa acttgtgtt atttgtttt 
      gagacatcg gatgcgggt tatttaaca gtttgcgct 
      gtctgtgct gatgcttac cacgcagga tgtcaccaa 
      ccgcgcatt catgagaaa tttattaaa ccaccggcg 
      aaatggctt tgcatcaat tgcgaaatg cccgaggaa 
      ctgaaaatc aatgaaatc cagtccaac ataagcagt 
      tctttcacg tctaaatta accaacggg aatgacaca 
      aacgataac aattcgtca tcaccttcc atttctgga 
      aatacaacc cctaattac caatcgccg cccttatca 
      cccacgccg cctcagctc agtccccaa aacgaagcg 
      ccacaaaac ggcgatgga ggggtttct gattctcag 
      agtaaccaa agtgttaaa gattccgat gatgataac 
      atagatccg tctatacct gatgcgacc aattggaca 
      atcgaagag gtgtatcag tatttcgct cagtatttc 
      cccgaggaa gccaaaatt tttaaggaa caggaaatt 
      gatggccgt tcgttgttg ttgctgaaa cgaatggac 
      gttttgaca aatttgaaa ctaaaactg ggacctgca 
      ctcaaaatc tacaagcat gttgttaag ctacaagtg 
      agaagggat gatccaaag ttctactgg ttgtaaata 
      ttgctttta acagttact ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY