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Gene information
General information
TC012280 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3745989..3751413
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012280
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01960

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    tropharium size decreased - Number of animals showing the phenotype: 4

    egg chamber number decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber potentially position irregular - Number of animals showing the phenotype: 4

    ovariole sometimes duplicated - Number of animals showing the phenotype: 4

    vitellogenic egg chamber sometimes differentiation irregular - Number of animals showing the phenotype: 4

    Comment: some of the egg chambers seem polynucleated
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012280 RA
      MNAINKNRD RILEAIDQL RRRKARPDI QRICNYLFR 
RFSISSVEA RSDLQWCVE NDIVLKVEY KGSISYRNA 
AKKFSHVKR DSQSEHNAD NKANRKFAQ LLTNAFGEL 
VVHEPDYLE YGVPAEEII DNILSKDSV RYTRKYISI 
LIEKEVENG GLIKMEHDR YLMGPTKER TDDKGASGE 
AKDGDTHSQ DLVISVKPR KKPGPKPGL KKEKSESMD 
SDEKKSETG SLRVGGRRK RAKKVFDPS DTHVPKKRG 
RPAGSLNKS TIEKQLAKL SDESRPESR TSNSGREQG 
GVCSVCHVQ NKMGPNDRM VACRECSNK AHYSCLNGD 
DMMLRMYPD NTWQCPHCK TCVICFETS DAGYLTVCA 
VCADAYHAG CHQPRIHEK FIKPPAKWL CINCEMPEE 
LKINEIQSN ISSSFTSKL TNGNDTNDN NSSSPSISG 
NTTPNYQSP PLSPTPPQL SPQNEAPQN GDGGVSDSQ 
SNQSVKDSD DDNIDPSIP DATNWTIEE VYQYFAQYF 
PEEAKIFKE QEIDGRSLL LLKRMDVLT NLKLKLGPA 
LKIYKHVVK LQVRRDDPK FYWL
    • >TC012280 RB
      MNAINKNRD RILEAIDQL RRRKARPDI QRICNYLFR 
RFSISSVEA RSDLQWCVE NDIVLKVEY KGSISYRNA 
AKKFSHVKR DSQSEHNAD NKANRKFAQ LLTNAFGEL 
VVHEPDYLE YGVPAEEII DNILSKDSV RYTRKYISI 
LIEKEVENG GLIKMEHDR YLMGPTKER TDDKGASGE 
AKDGDTHSQ DLVISVKPR KKPGPKPGL KKEKSESMD 
SDEKKSETG SLRVGGRRK TNKPKMRAK KVFDPSDTH 
VPKKRGRPA GSLNKSTIE KQLAKLSDE SRPESRTSN 
SGREQGGVC SVCHVQNKM GPNDRMVAC RECSNKAHY 
SCLNGDDMM LRMYPDNTW QCPHCKTCV ICFETSDAG 
YLTVCAVCA DAYHAGCHQ PRIHEKFIK PPAKWLCIN 
CEMPEELKI NEIQSNISS SFTSKLTNG NDTNDNNSS 
SPSISGNTT PNYQSPPLS PTPPQLSPQ NEAPQNGDG 
GVSDSQSNQ SVKDSDDDN IDPSIPDAT NWTIEEVYQ 
YFAQYFPEE AKIFKEQEI DGRSLLLLK RMDVLTNLK 
LKLGPALKI YKHVVKLQV RRDDPKFYW L
    CDS sequence
    • >TC012280 RA
      atgaacgcg attaacaag aaccgagac cgcattctg 
gaggcgatt gaccagctc aggcgccgc aaagcgcgc 
cccgacatc cagcgcatt tgcaattac ctcttccgg 
cggttttcc ataagttcg gtggaggcc cgctccgac 
ctgcaatgg tgcgtggag aacgacatc gtcctcaaa 
gtggagtac aaagggagc atcagttac aggaacgcg 
gcgaaaaag ttctcgcac gtgaaacgc gacagccag 
agcgagcac aacgccgac aacaaagcc aaccggaag 
ttcgcacaa ctcctcacg aacgccttc ggggagctg 
gtggtgcac gagcccgac tacctggag tacggcgtg 
ccggcggag gagatcatc gacaacatc ctctccaag 
gacagcgtg cgctacacc aggaagtac atctcgatc 
ctgatcgag aaggaggtg gagaacggc ggcctcatc 
aagatggaa catgacagg tacctgatg ggaccgacc 
aaagagagg acggacgat aagggcgcc agcggggaa 
gcaaaggac ggcgatacg cactcgcag gacctagtc 
attagcgtc aaaccccgg aaaaaaccg ggacccaaa 
cccggactc aaaaaggaa aagtccgaa agcatggat 
tcggacgag aaaaaatcc gaaacgggc agtctgcga 
gtaggggga aggcgaaag agggccaag aaagtgttc 
gacccgtcc gacacccac gtcccgaag aagcgcggc 
cgtccagct gggtccctc aataaatcg acaatcgag 
aagcaatta gcgaaattg agtgatgag tccaggcct 
gaatcgcgg acttcgaac agtggtcgg gaacagggt 
ggtgtttgt tctgtttgt catgtccag aacaaaatg 
gggcccaat gatcgcatg gtggcctgt cgggaatgt 
agtaataaa gctcattac agttgcttg aatggggac 
gatatgatg ctgaggatg tatccagat aatacgtgg 
caatgtccg cattgtaaa acttgtgtt atttgtttt 
gagacatcg gatgcgggt tatttaaca gtttgcgct 
gtctgtgct gatgcttac cacgcagga tgtcaccaa 
ccgcgcatt catgagaaa tttattaaa ccaccggcg 
aaatggctt tgcatcaat tgcgaaatg cccgaggaa 
ctgaaaatc aatgaaatc cagtccaac ataagcagt 
tctttcacg tctaaatta accaacggg aatgacaca 
aacgataac aattcgtca tcaccttcc atttctgga 
aatacaacc cctaattac caatcgccg cccttatca 
cccacgccg cctcagctc agtccccaa aacgaagcg 
ccacaaaac ggcgatgga ggggtttct gattctcag 
agtaaccaa agtgttaaa gattccgat gatgataac 
atagatccg tctatacct gatgcgacc aattggaca 
atcgaagag gtgtatcag tatttcgct cagtatttc 
cccgaggaa gccaaaatt tttaaggaa caggaaatt 
gatggccgt tcgttgttg ttgctgaaa cgaatggac 
gttttgaca aatttgaaa ctaaaactg ggacctgca 
ctcaaaatc tacaagcat gttgttaag ctacaagtg 
agaagggat gatccaaag ttctactgg ttgtaa
    • >TC012280 RB
      atgaacgcg attaacaag aaccgagac cgcattctg 
gaggcgatt gaccagctc aggcgccgc aaagcgcgc 
cccgacatc cagcgcatt tgcaattac ctcttccgg 
cggttttcc ataagttcg gtggaggcc cgctccgac 
ctgcaatgg tgcgtggag aacgacatc gtcctcaaa 
gtggagtac aaagggagc atcagttac aggaacgcg 
gcgaaaaag ttctcgcac gtgaaacgc gacagccag 
agcgagcac aacgccgac aacaaagcc aaccggaag 
ttcgcacaa ctcctcacg aacgccttc ggggagctg 
gtggtgcac gagcccgac tacctggag tacggcgtg 
ccggcggag gagatcatc gacaacatc ctctccaag 
gacagcgtg cgctacacc aggaagtac atctcgatc 
ctgatcgag aaggaggtg gagaacggc ggcctcatc 
aagatggaa catgacagg tacctgatg ggaccgacc 
aaagagagg acggacgat aagggcgcc agcggggaa 
gcaaaggac ggcgatacg cactcgcag gacctagtc 
attagcgtc aaaccccgg aaaaaaccg ggacccaaa 
cccggactc aaaaaggaa aagtccgaa agcatggat 
tcggacgag aaaaaatcc gaaacgggc agtctgcga 
gtaggggga aggcgaaag accaacaaa cccaaaatg 
agggccaag aaagtgttc gacccgtcc gacacccac 
gtcccgaag aagcgcggc cgtccagct gggtccctc 
aataaatcg acaatcgag aagcaatta gcgaaattg 
agtgatgag tccaggcct gaatcgcgg acttcgaac 
agtggtcgg gaacagggt ggtgtttgt tctgtttgt 
catgtccag aacaaaatg gggcccaat gatcgcatg 
gtggcctgt cgggaatgt agtaataaa gctcattac 
agttgcttg aatggggac gatatgatg ctgaggatg 
tatccagat aatacgtgg caatgtccg cattgtaaa 
acttgtgtt atttgtttt gagacatcg gatgcgggt 
tatttaaca gtttgcgct gtctgtgct gatgcttac 
cacgcagga tgtcaccaa ccgcgcatt catgagaaa 
tttattaaa ccaccggcg aaatggctt tgcatcaat 
tgcgaaatg cccgaggaa ctgaaaatc aatgaaatc 
cagtccaac ataagcagt tctttcacg tctaaatta 
accaacggg aatgacaca aacgataac aattcgtca 
tcaccttcc atttctgga aatacaacc cctaattac 
caatcgccg cccttatca cccacgccg cctcagctc 
agtccccaa aacgaagcg ccacaaaac ggcgatgga 
ggggtttct gattctcag agtaaccaa agtgttaaa 
gattccgat gatgataac atagatccg tctatacct 
gatgcgacc aattggaca atcgaagag gtgtatcag 
tatttcgct cagtatttc cccgaggaa gccaaaatt 
tttaaggaa caggaaatt gatggccgt tcgttgttg 
ttgctgaaa cgaatggac gttttgaca aatttgaaa 
ctaaaactg ggacctgca ctcaaaatc tacaagcat 
gttgttaag ctacaagtg agaagggat gatccaaag 
ttctactgg ttgtaa
    mRNA sequence
    • >TC012280 RA
      tgcgtggta tgaaccgct cggtttccg caagaaata 
ggcgacgga cgggccgcc aacccgccc caaataatc 
gcaaacagt taggggaag cgatcggtg ccgggccca 
gcgcccgca agggccgcg ttctcgcgc ttttccgtg 
tgcgtttcc cggtgcgaa atgaacgcg attaacaag 
aaccgagac cgcattctg gaggcgatt gaccagctc 
aggcgccgc aaagcgcgc cccgacatc cagcgcatt 
tgcaattac ctcttccgg cggttttcc ataagttcg 
gtggaggcc cgctccgac ctgcaatgg tgcgtggag 
aacgacatc gtcctcaaa gtggagtac aaagggagc 
atcagttac aggaacgcg gcgaaaaag ttctcgcac 
gtgaaacgc gacagccag agcgagcac aacgccgac 
aacaaagcc aaccggaag ttcgcacaa ctcctcacg 
aacgccttc ggggagctg gtggtgcac gagcccgac 
tacctggag tacggcgtg ccggcggag gagatcatc 
gacaacatc ctctccaag gacagcgtg cgctacacc 
aggaagtac atctcgatc ctgatcgag aaggaggtg 
gagaacggc ggcctcatc aagatggaa catgacagg 
tacctgatg ggaccgacc aaagagagg acggacgat 
aagggcgcc agcggggaa gcaaaggac ggcgatacg 
cactcgcag gacctagtc attagcgtc aaaccccgg 
aaaaaaccg ggacccaaa cccggactc aaaaaggaa 
aagtccgaa agcatggat tcggacgag aaaaaatcc 
gaaacgggc agtctgcga gtaggggga aggcgaaag 
agggccaag aaagtgttc gacccgtcc gacacccac 
gtcccgaag aagcgcggc cgtccagct gggtccctc 
aataaatcg acaatcgag aagcaatta gcgaaattg 
agtgatgag tccaggcct gaatcgcgg acttcgaac 
agtggtcgg gaacagggt ggtgtttgt tctgtttgt 
catgtccag aacaaaatg gggcccaat gatcgcatg 
gtggcctgt cgggaatgt agtaataaa gctcattac 
agttgcttg aatggggac gatatgatg ctgaggatg 
tatccagat aatacgtgg caatgtccg cattgtaaa 
acttgtgtt atttgtttt gagacatcg gatgcgggt 
tatttaaca gtttgcgct gtctgtgct gatgcttac 
cacgcagga tgtcaccaa ccgcgcatt catgagaaa 
tttattaaa ccaccggcg aaatggctt tgcatcaat 
tgcgaaatg cccgaggaa ctgaaaatc aatgaaatc 
cagtccaac ataagcagt tctttcacg tctaaatta 
accaacggg aatgacaca aacgataac aattcgtca 
tcaccttcc atttctgga aatacaacc cctaattac 
caatcgccg cccttatca cccacgccg cctcagctc 
agtccccaa aacgaagcg ccacaaaac ggcgatgga 
ggggtttct gattctcag agtaaccaa agtgttaaa 
gattccgat gatgataac atagatccg tctatacct 
gatgcgacc aattggaca atcgaagag gtgtatcag 
tatttcgct cagtatttc cccgaggaa gccaaaatt 
tttaaggaa caggaaatt gatggccgt tcgttgttg 
ttgctgaaa cgaatggac gttttgaca aatttgaaa 
ctaaaactg ggacctgca ctcaaaatc tacaagcat 
gttgttaag ctacaagtg agaagggat gatccaaag 
ttctactgg ttgtaaata ttgctttta acagttact 
ttg
    • >TC012280 RB
      tgcgtggta tgaaccgct cggtttccg caagaaata 
ggcgacgga cgggccgcc aacccgccc caaataatc 
gcaaacagt taggggaag cgatcggtg ccgggccca 
gcgcccgca agggccgcg ttctcgcgc ttttccgtg 
tgcgtttcc cggtgcgaa atgaacgcg attaacaag 
aaccgagac cgcattctg gaggcgatt gaccagctc 
aggcgccgc aaagcgcgc cccgacatc cagcgcatt 
tgcaattac ctcttccgg cggttttcc ataagttcg 
gtggaggcc cgctccgac ctgcaatgg tgcgtggag 
aacgacatc gtcctcaaa gtggagtac aaagggagc 
atcagttac aggaacgcg gcgaaaaag ttctcgcac 
gtgaaacgc gacagccag agcgagcac aacgccgac 
aacaaagcc aaccggaag ttcgcacaa ctcctcacg 
aacgccttc ggggagctg gtggtgcac gagcccgac 
tacctggag tacggcgtg ccggcggag gagatcatc 
gacaacatc ctctccaag gacagcgtg cgctacacc 
aggaagtac atctcgatc ctgatcgag aaggaggtg 
gagaacggc ggcctcatc aagatggaa catgacagg 
tacctgatg ggaccgacc aaagagagg acggacgat 
aagggcgcc agcggggaa gcaaaggac ggcgatacg 
cactcgcag gacctagtc attagcgtc aaaccccgg 
aaaaaaccg ggacccaaa cccggactc aaaaaggaa 
aagtccgaa agcatggat tcggacgag aaaaaatcc 
gaaacgggc agtctgcga gtaggggga aggcgaaag 
accaacaaa cccaaaatg agggccaag aaagtgttc 
gacccgtcc gacacccac gtcccgaag aagcgcggc 
cgtccagct gggtccctc aataaatcg acaatcgag 
aagcaatta gcgaaattg agtgatgag tccaggcct 
gaatcgcgg acttcgaac agtggtcgg gaacagggt 
ggtgtttgt tctgtttgt catgtccag aacaaaatg 
gggcccaat gatcgcatg gtggcctgt cgggaatgt 
agtaataaa gctcattac agttgcttg aatggggac 
gatatgatg ctgaggatg tatccagat aatacgtgg 
caatgtccg cattgtaaa acttgtgtt atttgtttt 
gagacatcg gatgcgggt tatttaaca gtttgcgct 
gtctgtgct gatgcttac cacgcagga tgtcaccaa 
ccgcgcatt catgagaaa tttattaaa ccaccggcg 
aaatggctt tgcatcaat tgcgaaatg cccgaggaa 
ctgaaaatc aatgaaatc cagtccaac ataagcagt 
tctttcacg tctaaatta accaacggg aatgacaca 
aacgataac aattcgtca tcaccttcc atttctgga 
aatacaacc cctaattac caatcgccg cccttatca 
cccacgccg cctcagctc agtccccaa aacgaagcg 
ccacaaaac ggcgatgga ggggtttct gattctcag 
agtaaccaa agtgttaaa gattccgat gatgataac 
atagatccg tctatacct gatgcgacc aattggaca 
atcgaagag gtgtatcag tatttcgct cagtatttc 
cccgaggaa gccaaaatt tttaaggaa caggaaatt 
gatggccgt tcgttgttg ttgctgaaa cgaatggac 
gttttgaca aatttgaaa ctaaaactg ggacctgca 
ctcaaaatc tacaagcat gttgttaag ctacaagtg 
agaagggat gatccaaag ttctactgg ttgtaaata 
ttgctttta acagttact ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY