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Gene information
General information
TC012271 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3470482..3473710
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC012271
      iBeetle screen iB_01959

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012271 RA
        MAHLYAAYR CNFCCELPE TAPIYHCPN RHQYCNECF 
RKLQRLYRG NVEGGGCCL ICKCAAKFE KSDVSSELL 
KKLKVKPGI GRPLKGNPR KSMFFDDGE ISTAENMFG 
SQLEKNQGD GLKSLKTRS MSATTMSKR PLTCLHDGC 
RKSVAVSSL VSHFKHDHP AVPCFGIER GRELKMLFD 
ISLVAHNRT FCLSMVTVY EFNKIDVVR SKSSQSVIN 
TCSKLSGRI PLNTFWLMM SGSSEYRKE NSYVVFWLY 
SNCEDRYWY TIELSSKND RISDSSFCS AVGLHESRT 
VEDVARSMN CLFVNYGSF IALLEEGDK INLRITVH
      CDS sequence
      • >TC012271 RA
        atggctcat ctgtacgcg gcgtatagg tgtaatttc 
tgctgtgag cttccggag acagcaccg atctaccat 
tgcccaaac agacatcaa tactgcaat gaatgcttc 
agaaagttg caaaggctt tatcgtggg aacgttgaa 
ggcggaggc tgttgtttg atttgtaaa tgtgctgcc 
aagtttgag aaaagtgat gtgagttcg gagttgttg 
aaaaaactt aaggttaaa cccggaatc ggacgacca 
ctaaaaggt aacccgagg aagagtatg tttttcgat 
gatggtgaa atttctaca gctgaaaat atgtttggc 
tcacagctt gaaaaaaac caaggcgat ggcttaaaa 
tctttgaaa acgcgatca atgagtgcc accacaatg 
tcaaaacga cctctgacg tgtcttcac gatggttgt 
agaaaatca gtggctgtt tcctccctt gtgagccat 
ttcaagcac gaccatccc gcagtcccc tgctttgga 
atcgaaagg gggcgagag ttaaaaatg ttatttgac 
atttcgcta gtggcacac aatcgaact ttctgtctg 
tcgatggtc acggtttac gaattcaac aaaatcgac 
gtcgttcgt tcgaaaagc tcccaaagt gttatcaat 
acctgcagt aagctttct ggtagaatc ccacttaac 
accttctgg ctaatgatg agcggttcg agtgaatac 
agaaaggag aattcgtat gtcgttttt tggctttat 
tctaattgt gaagatcgg tattggtac acgattgag 
ctgtcgtcg aaaaacgat agaattagt gattcgagc 
ttttgcagc gctgttgga ttgcatgaa agtcgcacc 
gtggaagac gtcgccaga agtatgaac tgcctcttt 
gtcaattat gggtccttt atagccctt cttgaagaa 
ggggataaa attaattta agaattaca gtacattaa
      mRNA sequence
      • >TC012271 RA
        ttgtcaaaa tttcataaa cacacgtca gaaagcaaa 
attttttgt atcattctt ccaaatctg gcagagtaa 
aggaatggc tcatctgta cgcggcgta taggtgtaa 
tttctgctg tgagcttcc ggagacagc accgatcta 
ccattgccc aaacagaca tcaatactg caatgaatg 
cttcagaaa gttgcaaag gctttatcg tgggaacgt 
tgaaggcgg aggctgttg tttgatttg taaatgtgc 
tgccaagtt tgagaaaag tgatgtgag ttcggagtt 
gttgaaaaa acttaaggt taaacccgg aatcggacg 
accactaaa aggtaaccc gaggaagag tatgttttt 
cgatgatgg tgaaatttc tacagctga aaatatgtt 
tggctcaca gcttgaaaa aaaccaagg cgatggctt 
aaaatcttt gaaaacgcg atcaatgag tgccaccac 
aatgtcaaa acgacctct gacgtgtct tcacgatgg 
ttgtagaaa atcagtggc tgtttcctc ccttgtgag 
ccatttcaa gcacgacca tcccgcagt cccctgctt 
tggaatcga aagggggcg agagttaaa aatgttatt 
tgacatttc gctagtggc acacaatcg aactttctg 
tctgtcgat ggtcacggt ttacgaatt caacaaaat 
cgacgtcgt tcgttcgaa aagctccca aagtgttat 
caatacctg cagtaagct ttctggtag aatcccact 
taacacctt ctggctaat gatgagcgg ttcgagtga 
atacagaaa ggagaattc gtatgtcgt tttttggct 
ttattctaa ttgtgaaga tcggtattg gtacacgat 
tgagctgtc gtcgaaaaa cgatagaat tagtgattc 
gagcttttg cagcgctgt tggattgca tgaaagtcg 
caccgtgga agacgtcgc cagaagtat gaactgcct 
ctttgtcaa ttatgggtc ctttatagc ccttcttga 
agaagggga taaaattaa tttaagaat tacagtaca 
ttaagttta aattgtctg tgccttttt aactcgtgt 
ctcgatgga agctcacac aataaaaga ttttttaag 
ac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY