Status
Please wait ...
Gene information
General information
TC012222 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 172987..180235
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012222
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03178

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_01952

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012222 RA
      MAEDGIDLI REILVDVEL SQFLLPIRD DLQITRLEH 
      FDYVKAEDL ENIGLSKPG ARRLLEAVK KKRAQIRKR 
      NLINKLIPV VANKTATLK KTDDSVTLA DFTSCLIPE 
      SNITLSIKL GDGSFGVVR RGEWTSPNG RVLPVAVKI 
      LKADALSQP GVFEDFIKE VQAMHVLSH PNLIRLYGV 
      VLSQPMMMV TELANLGSL LDYLRKQCQ QTPVPMLCE 
      YATQVATGM AYLESKRFL HRDLACRNV LLASADKIK 
      IGDFGLMRA LPQEEDCYV MTEHKKVPF PWCAPESLR 
      SRQFSHASD TWMFGVTVW EMFTFGEDP WMGLIGSEI 
      LRKIDKEGE RLHQPEACP PAVYSILLQ CWLKNPTER 
      PTFAALKEF FRKNKTPVM KAVGGQDEP NKLKIEEGD 
      EIAIIDGSA ELYWWKGQN QRTFEIGLF ARCLVDPMR 
      PKQPEDISK PLQNSFIHT GHGSAFGES WGSPSYIDD 
      MYLRNPMEP PDVLGLHRE AKPSPQLCD RTKSIKNTN 
      SLKQKPSDK QFNYRKLTN ESSIKMKPQ RPPQPKLEK 
      NREGVLIDL SPEMGLSKQ SNVGSADSR SVSILDEPI 
      DVMQEYEYP PSNAPPPYQ QPPSYYNTK MLEQLTDDP 
      FDTSRVYSP EPRQPYSEI PTTYSNNFN SNNEIKQNG 
      YIKGDIANR GLNYGSDIQ VVTSKGVLT TPKEVATPV 
      PSTNDVSLM NLNQVPSTN DTKFVQELE KYLLSKPEV 
      PVLQPPPQS TKKPDPVLP KVQNGTYSN LDTYSKGSK 
      YDTTNVFNR IWHENMVKE NGNVKQCKN FDIPPTSTS 
      QNFYHHYDP VSDFDRLSV SGVNFYDNC HAAADNLYN 
      NTSFLGQPS SSLSSFSSC NTQTAVYGS YNSIRQYSE 
      VTDAVYSEI AENSYSQVP EESLKPHRP APPSPMVQS 
      MQQIQRKIQ QGQLSADAE RLMTSEYRN NKISQVREC 
      VPDISTSDC LQALQLCGW EVAATVKHV KIDKLLKLG 
      LASRERCEA ALQRTNWNV ELAASAILD S
    CDS sequence
    • >TC012222 RA
      atggccgaa gatggtatt gacttgatc cgcgagata 
      ctagtagat gtagaattg agccagttt ttgttaccc 
      ataagagat gatctccaa ataacgcga ctagaacat 
      ttcgattac gtgaaggcc gaggacttg gaaaacatc 
      ggattaagc aaaccaggt gctagacgg ttgctggaa 
      gccgtcaag aaaaaacgg gcgcagatt aggaaacgc 
      aacttaatc aataagctg atcccggtc gttgctaat 
      aagacagcg actttgaaa aaaactgac gacagtgtg 
      actttggcg gacttcacc tcttgtctc atccccgaa 
      tctaacata actttgtca attaagctg ggcgatggg 
      tcgtttggg gttgtgcga aggggcgaa tggacgagt 
      cccaacggt cgagtgctt ccagtcgct gttaagatc 
      ctcaaagcc gatgcgtta tcccaaccg ggggttttc 
      gaggacttc atcaaggaa gtccaagct atgcatgtg 
      ctaagtcac cccaattta atccgtctg tatggcgtg 
      gtgctgtcg cagcccatg atgatggtg accgagtta 
      gcaaatcta gggtctcta ttagactac ctcaggaaa 
      cagtgccaa caaacaccc gtgcccatg ctgtgcgag 
      tatgccacg caagtggcc accgggatg gcgtacctg 
      gagagcaag cggtttctg caccgggac ctcgcctgc 
      cggaacgtc ctgctagcc tcagctgat aagatcaaa 
      atcggtgac ttcggcttg atgcgcgct ttgccccaa 
      gaggaagac tgttacgtc atgacggag cacaagaag 
      gtgccgttc ccgtggtgc gcccccgag tcgttgcgc 
      tcgaggcag ttcagccac gcgtccgat acgtggatg 
      ttcggggtg acggtgtgg gagatgttc acgttcggg 
      gaggacccg tggatgggg ctcatcggt tcggagatt 
      ttgcggaaa attgataag gagggagag aggctgcac 
      cagccggag gcgtgtccg ccggctgtt tacagtatt 
      ttgctgcag tgttggctc aaaaatccg accgaacgg 
      ccgactttc gctgcgttg aaggagttt ttccggaag 
      aataaaaca ccagtgatg aaggcggtt gggggacag 
      gacgagccg aataaattg aagattgag gaaggggat 
      gagattgcg attattgat gggagtgca gagttgtat 
      tggtggaaa ggacagaat cagaggacg tttgagatc 
      gggttgttt gccagatgt ttggtcgac cccatgcga 
      cccaaacaa ccggaagac atcagcaaa cccctccag 
      aattccttc atccacaca ggtcacggt tcagccttt 
      ggcgaatcg tggggcagt ccgtcctat attgacgac 
      atgtacctg agaaatccc atggagccc cccgacgtc 
      ctcggtttg caccgtgaa gccaaacct tctccacag 
      ctatgtgat agaaccaaa agtatcaaa aacacaaat 
      agtctcaaa caaaagcca agtgataaa caattcaat 
      tatcggaaa ctaacgaac gaatctagc attaaaatg 
      aaacctcaa aggccccca cagccaaaa ttagagaaa 
      aatcgggaa ggtgttttg attgatttg tcaccagag 
      atgggtttg tccaaacag agcaatgtt ggcagtgct 
      gattcgcga agtgtctca atcttggat gagccaata 
      gacgtgatg caagagtat gagtaccca ccatcaaat 
      gccccacca ccgtaccag cagcccccc tcatactac 
      aacactaaa atgctcgaa caattgacc gatgacccg 
      ttcgatact tctcgggtc tactccccg gaaccccgg 
      cagccctac tctgaaata cccacaaca tactcaaat 
      aacttcaac tcgaataac gaaatcaaa caaaacggg 
      tacatcaaa ggtgatata gcgaaccgt ggcttgaac 
      tacggaagc gacatccaa gtcgtgacc tccaaaggc 
      gtcctaacc acccccaag gaagttgca accccagtt 
      ccaagcaca aacgacgtg tctttgatg aatctcaac 
      caagtgcca agtactaac gacacgaaa ttcgtccaa 
      gaactcgag aaatacctc ctgtctaaa cccgaagtc 
      ccagttttg caaccccct ccccaaagc acgaaaaaa 
      cccgaccca gtcctcccc aaagtccaa aacgggact 
      tactcgaat ctagatact tacagcaaa ggctcaaag 
      tacgacacg acgaacgtt ttcaatcga atttggcac 
      gaaaatatg gtcaaagaa aacgggaac gtgaaacag 
      tgcaaaaac tttgacatt cccccgact tctactagt 
      cagaatttt taccatcac tacgaccca gtctcggat 
      tttgatcgg ctgtccgtt tctggcgtc aatttttat 
      gataattgt cacgcagcg gccgataat ttgtataat 
      aatacgtcg tttttgggg caaccgagc tcgtcgctt 
      agttccttc agttcgtgc aatactcag accgccgtt 
      tacggatcg tataattcg ataaggcag tatagcgaa 
      gttaccgat gcggtgtat tcggaaatt gccgaaaat 
      tcatacagt caggtgcct gaggagagt ctcaagccg 
      cataggccg gcgccaccg tcgcccatg gtgcagagc 
      atgcagcaa atccagcgc aagatacaa caagggcag 
      ctgagcgct gacgccgag cgccttatg acgtccgaa 
      taccgcaat aacaagata agtcaagtc cgggaatgc 
      gtgcctgat atcagcacc agcgactgc ttacaagcc 
      ttgcaacta tgtgggtgg gaggtggcc gccacggtg 
      aaacacgtg aagatcgat aaattactc aagctagga 
      ttggccagt cgtgagcga tgcgaggcg gctttacaa 
      cgtacaaac tggaatgtc gagttggca gcgtcggct 
      attttagac agttga
    mRNA sequence
    • >TC012222 RA
      cccgactta tcttgtggt tttttaatt gtttatatt 
      agtaaaagt gattgttta aacaaattt gcgataaaa 
      ttttattgc aaataaagc atgaatggc cgttgaact 
      ttaagaatc agtaatgcg tattacata aaaacacta 
      aaatggccg aagatggta ttgacttga tccgcgaga 
      tactagtag atgtagaat tgagccagt ttttgttac 
      ccataagag atgatctcc aaataacgc gactagaac 
      atttcgatt acgtgaagg ccgaggact tggaaaaca 
      tcggattaa gcaaaccag gtgctagac ggttgctgg 
      aagccgtca agaaaaaac gggcgcaga ttaggaaac 
      gcaacttaa tcaataagc tgatcccgg tcgttgcta 
      ataagacag cgactttga aaaaaactg acgacagtg 
      tgactttgg cggacttca cctcttgtc tcatccccg 
      aatctaaca taactttgt caattaagc tgggcgatg 
      ggtcgtttg gggttgtgc gaaggggcg aatggacga 
      gtcccaacg gtcgagtgc ttccagtcg ctgttaaga 
      tcctcaaag ccgatgcgt tatcccaac cgggggttt 
      tcgaggact tcatcaagg aagtccaag ctatgcatg 
      tgctaagtc accccaatt taatccgtc tgtatggcg 
      tggtgctgt cgcagccca tgatgatgg tgaccgagt 
      tagcaaatc tagggtctc tattagact acctcagga 
      aacagtgcc aacaaacac ccgtgccca tgctgtgcg 
      agtatgcca cgcaagtgg ccaccggga tggcgtacc 
      tggagagca agcggtttc tgcaccggg acctcgcct 
      gccggaacg tcctgctag cctcagctg ataagatca 
      aaatcggtg acttcggct tgatgcgcg ctttgcccc 
      aagaggaag actgttacg tcatgacgg agcacaaga 
      aggtgccgt tcccgtggt gcgcccccg agtcgttgc 
      gctcgaggc agttcagcc acgcgtccg atacgtgga 
      tgttcgggg tgacggtgt gggagatgt tcacgttcg 
      gggaggacc cgtggatgg ggctcatcg gttcggaga 
      ttttgcgga aaattgata aggagggag agaggctgc 
      accagccgg aggcgtgtc cgccggctg tttacagta 
      ttttgctgc agtgttggc tcaaaaatc cgaccgaac 
      ggccgactt tcgctgcgt tgaaggagt ttttccgga 
      agaataaaa caccagtga tgaaggcgg ttgggggac 
      aggacgagc cgaataaat tgaagattg aggaagggg 
      atgagattg cgattattg atgggagtg cagagttgt 
      attggtgga aaggacaga atcagagga cgtttgaga 
      tcgggttgt ttgccagat gtttggtcg accccatgc 
      gacccaaac aaccggaag acatcagca aacccctcc 
      agaattcct tcatccaca caggtcacg gttcagcct 
      ttggcgaat cgtggggca gtccgtcct atattgacg 
      acatgtacc tgagaaatc ccatggagc cccccgacg 
      tcctcggtt tgcaccgtg aagccaaac cttctccac 
      agctatgtg atagaacca aaagtatca aaaacacaa 
      atagtctca aacaaaagc caagtgata aacaattca 
      attatcgga aactaacga acgaatcta gcattaaaa 
      tgaaacctc aaaggcccc cacagccaa aattagaga 
      aaaatcggg aaggtgttt tgattgatt tgtcaccag 
      agatgggtt tgtccaaac agagcaatg ttggcagtg 
      ctgattcgc gaagtgtct caatcttgg atgagccaa 
      tagacgtga tgcaagagt atgagtacc caccatcaa 
      atgccccac caccgtacc agcagcccc cctcatact 
      acaacacta aaatgctcg aacaattga ccgatgacc 
      cgttcgata cttctcggg tctactccc cggaacccc 
      ggcagccct actctgaaa tacccacaa catactcaa 
      ataacttca actcgaata acgaaatca aacaaaacg 
      ggtacatca aaggtgata tagcgaacc gtggcttga 
      actacggaa gcgacatcc aagtcgtga cctccaaag 
      gcgtcctaa ccaccccca aggaagttg caaccccag 
      ttccaagca caaacgacg tgtctttga tgaatctca 
      accaagtgc caagtacta acgacacga aattcgtcc 
      aagaactcg agaaatacc tcctgtcta aacccgaag 
      tcccagttt tgcaacccc ctccccaaa gcacgaaaa 
      aacccgacc cagtcctcc ccaaagtcc aaaacggga 
      cttactcga atctagata cttacagca aaggctcaa 
      agtacgaca cgacgaacg ttttcaatc gaatttggc 
      acgaaaata tggtcaaag aaaacggga acgtgaaac 
      agtgcaaaa actttgaca ttcccccga cttctacta 
      gtcagaatt tttaccatc actacgacc cagtctcgg 
      attttgatc ggctgtccg tttctggcg tcaattttt 
      atgataatt gtcacgcag cggccgata atttgtata 
      ataatacgt cgtttttgg ggcaaccga gctcgtcgc 
      ttagttcct tcagttcgt gcaatactc agaccgccg 
      tttacggat cgtataatt cgataaggc agtatagcg 
      aagttaccg atgcggtgt attcggaaa ttgccgaaa 
      attcataca gtcaggtgc ctgaggaga gtctcaagc 
      cgcataggc cggcgccac cgtcgccca tggtgcaga 
      gcatgcagc aaatccagc gcaagatac aacaagggc 
      agctgagcg ctgacgccg agcgcctta tgacgtccg 
      aataccgca ataacaaga taagtcaag tccgggaat 
      gcgtgcctg atatcagca ccagcgact gcttacaag 
      ccttgcaac tatgtgggt gggaggtgg ccgccacgg 
      tgaaacacg tgaagatcg ataaattac tcaagctag 
      gattggcca gtcgtgagc gatgcgagg cggctttac 
      aacgtacaa actggaatg tcgagttgg cagcgtcgg 
      ctattttag acagttgaa tttaaatag gaatcaaat 
      aaccgtgcc aaagatatt atcacagat agttaaatt 
      agttttaga ttgttaatt agcaacatg acatgaccg 
      cttgtgata atttgaatt tagtttttt ttaatactt 
      ccttttttt tctcgtagt gaggtgtgc cagttatcg 
      aattagctt tacctaaaa agggcacct cttgatgtt 
      ctacaatcg gctcgtttg gtttttaat aaaccaata 
      ggatcatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY