Status
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Gene information
General information
TC012219 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4644399..4647182
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012219
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01951

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012219 RA
      MNFSTLCDF LDSVRSAKG VPEKSKLTK TFVKSLRDQ 
      QQDLFSVLR LVVPKLDRE RPSYRLKES KIAKLIIKM 
      LDLPNGGDK TVLQKGIAG TTVDFVDVI HSVLQKYIV 
      NKEIGLSIR AINDFLDQI ANRSSDSGL DDLVLQFLL 
      KLSPRNIKW LILIILKDL KLGFGDNSI LNCFHPDGA 
      DFFATNSNL RNFCDLIRD PQVRLNELE INVFQAFRP 
      MLSKRCDAA NFKKCFPES KTFIIENKF DGERFQLHM 
      ADGQFRYFS RNGFDYTDT YGASFTAGI FTPKLRPVL 
      GPETKRVIL DGEMMLWNR ETRSFGSKG MNLDVKKLG 
      EGGKYQPCF CVFDILLHN DRVLTNQPL FKRLKCLKS 
      VVKNPVEGT IVVSQYSEA SSLGDIVDA LNSSVDNNE 
      EGIVVKDTK SVYKCSDRN SGWFKVKME YFDDVVHDL 
      DVILMGGCY SSGKLNSFF VGVSSGANT YLSFGRISS 
      GLSDEQLDL LAEKFQSKG VDFKSFSTE SEGKLQFGR 
      DRPDLYIEP HNSCILQIR ATELIRTTN DTVKCPYTL 
      RFPRVLKIR DDKPVDECF SINELLELA GQNKPVIKL 
      NKRHIELSE ISAKARPAK KIKLEVIKS DLLTESGDF 
      LTGKRFYVD SGTQKWGLD DIYHAIKKA GGEVSYRVE 
      PNVDVILVS KVGKKVAEL MKQPNHFDI VHVDWLHRV 
      MEYRELVDY KPGEMFYKG ANFRRDVGS DSDRFGDSF 
      REEATKESL KCAVRVMQE AGDFLNTNG AVFCGDKPS 
      FGDYVAYFD CFEEINNPR SKRIYYSLP DEAEFEFYS 
      GTVVKEITA QVNLIIIAE NNEDRVSIV SDFLANEGL 
      GTVDIVSKD FLYSRISSQ N
    CDS sequence
    • >TC012219 RA
      atgaacttc tccaccctt tgtgatttc ctggactcc 
      gtcaggagc gccaaaggc gtaccagaa aaatccaaa 
      ctaaccaaa accttcgtc aaatctctc cgtgatcag 
      cagcaagac ctattttcc gtgttaagg ttagtcgtg 
      ccaaagtta gaccgcgag cggccttcc taccgccta 
      aaagagtcc aaaatcgcc aaactaata atcaaaatg 
      ctagacctg cccaacggg ggcgacaag acggtgcta 
      cagaagggc atcgcgggc acaaccgtc gatttcgtc 
      gacgtaatt cactcagtt ttgcaaaaa tacatcgtc 
      aacaaagaa atcggtctg agcattcgc gccataaac 
      gacttcctg gaccaaatc gcaaaccgg tccagcgat 
      tcgggcttg gacgacctc gtcttgcaa ttcctcctc 
      aaactatcg ccacgcaac ataaagtgg ctcattctc 
      atcattctc aaagacctc aaactcggc tttggcgac 
      aactcgatc ttgaactgc ttccacccg gacggggcc 
      gattttttc gccacgaac agcaacttg aggaacttt 
      tgcgacctt attcgcgac ccccaagtc aggctgaac 
      gagctggag ataaatgtt ttccaggcg tttagaccg 
      atgctttca aaacggtgc gacgccgcc aacttcaaa 
      aagtgcttc cccgagtcg aaaactttc atcattgag 
      aataagttc gatggtgaa aggtttcaa ctgcacatg 
      gctgacggc cagtttagg tatttctcg cggaacggt 
      ttcgattac actgacacg tacggggcg tctttcacc 
      gcggggatt ttcaccccg aagttgcgc cctgttctt 
      ggccctgaa accaaacgg gtgattctg gacggggag 
      atgatgctc tggaacagg gaaacgcgc agtttcggg 
      tccaagggg atgaacttg gatgtgaaa aaattgggg 
      gagggcgga aaataccaa ccttgtttt tgcgtcttt 
      gatattttg ttgcacaac gaccgggtt ttgaccaat 
      cagccgctg tttaagcgg ctgaagtgc ctcaaaagt 
      gtggtcaaa aacccggtg gaaggcact attgttgtg 
      agtcaatac agtgaagcc agttctttg ggtgatatc 
      gtcgatgct ttgaattcg tcggttgat aataacgag 
      gaagggatt gtagttaaa gacaccaaa tcggtttac 
      aaatgcagc gaccggaac agcggttgg tttaaggtc 
      aaaatggaa tacttcgac gatgttgtt cacgatttg 
      gacgtgatt ttgatgggg ggttgttac agttcgggc 
      aaactcaat tcttttttt gttggggtc agttcaggg 
      gctaacacc tacttgtcc tttgggaga atctcgtcc 
      ggtttgagt gacgagcag ctcgacctt ttggccgaa 
      aaatttcag tcgaaaggg gttgatttc aagtcattt 
      tcaaccgag agcgagggc aagctccag ttcggccgt 
      gaccgccct gacctctac atcgagccc cacaattcg 
      tgcattttg caaatccga gccactgaa ttaatccgg 
      accacgaat gacacggtc aagtgtccc tacacttta 
      cgttttccc cgtgttttg aaaatccgg gacgacaaa 
      ccggtcgat gagtgcttc tcaataaac gagctcctc 
      gaactcgcg ggccagaac aagcccgtt atcaaactg 
      aacaaaaga cacatcgaa ctctctgaa ataagcgcc 
      aaggcaaga ccggcgaaa aaaatcaag cttgaggtc 
      atcaagtcg gatttgttg accgaatcc ggggatttt 
      ttaaccgga aagcgcttt tacgtcgat tccgggacc 
      cagaagtgg gggcttgat gacatctac cacgcgatc 
      aagaaagcc gggggtgag gtaagctac cgcgtggaa 
      ccgaacgtt gatgtgatt ttggtgtcg aaagttggt 
      aaaaaagtg gccgagctg atgaaacaa cccaatcat 
      tttgatata gtccacgtt gattggctg caccgcgtt 
      atggaatac cgggagttg gtggactac aagccgggg 
      gagatgttc tacaaaggg gctaatttt cggagagac 
      gttggctca gattcggat agatttggc gattcgttc 
      cgtgaggaa gcgacgaaa gagtcgcta aagtgtgcg 
      gtacgggtg atgcaagaa gcgggcgat tttttgaat 
      acaaacggg gcggttttt tgcggcgat aagccgagt 
      tttggtgat tatgtcgcg tattttgat tgtttcgaa 
      gagattaat aatccgcgt tccaaacgc atttattat 
      tcgcttcca gatgaagcg gagtttgag ttttatagt 
      ggtactgtg gttaaagag ataacggcg caagttaat 
      ttgataata attgcggaa aataacgag gatcgtgtc 
      tcaattgtt agtgatttt ttggcaaat gaaggactc 
      gggacggtt gatattgtg tcgaaagat ttcttgtac 
      agtcgcatt tctagtcaa aattaa
    mRNA sequence
    • >TC012219 RA
      tttatttat ttatgacat acgaggtcg tattaagaa 
      gttttaatt tgtaaaaaa taaacatga agataatgg 
      gagcatttt atttgtgaa actagaact gaagatttt 
      tttattaca ttatcgaaa tgaacttct ccacccttt 
      gtgatttcc tggactccg tcaggagcg ccaaaggcg 
      taccagaaa aatccaaac taaccaaaa ccttcgtca 
      aatctctcc gtgatcagc agcaagacc tattttccg 
      tgttaaggt tagtcgtgc caaagttag accgcgagc 
      ggccttcct accgcctaa aagagtcca aaatcgcca 
      aactaataa tcaaaatgc tagacctgc ccaacgggg 
      gcgacaaga cggtgctac agaagggca tcgcgggca 
      caaccgtcg atttcgtcg acgtaattc actcagttt 
      tgcaaaaat acatcgtca acaaagaaa tcggtctga 
      gcattcgcg ccataaacg acttcctgg accaaatcg 
      caaaccggt ccagcgatt cgggcttgg acgacctcg 
      tcttgcaat tcctcctca aactatcgc cacgcaaca 
      taaagtggc tcattctca tcattctca aagacctca 
      aactcggct ttggcgaca actcgatct tgaactgct 
      tccacccgg acggggccg attttttcg ccacgaaca 
      gcaacttga ggaactttt gcgacctta ttcgcgacc 
      cccaagtca ggctgaacg agctggaga taaatgttt 
      tccaggcgt ttagaccga tgctttcaa aacggtgcg 
      acgccgcca acttcaaaa agtgcttcc ccgagtcga 
      aaactttca tcattgaga ataagttcg atggtgaaa 
      ggtttcaac tgcacatgg ctgacggcc agtttaggt 
      atttctcgc ggaacggtt tcgattaca ctgacacgt 
      acggggcgt ctttcaccg cggggattt tcaccccga 
      agttgcgcc ctgttcttg gccctgaaa ccaaacggg 
      tgattctgg acggggaga tgatgctct ggaacaggg 
      aaacgcgca gtttcgggt ccaagggga tgaacttgg 
      atgtgaaaa aattggggg agggcggaa aataccaac 
      cttgttttt gcgtctttg atattttgt tgcacaacg 
      accgggttt tgaccaatc agccgctgt ttaagcggc 
      tgaagtgcc tcaaaagtg tggtcaaaa acccggtgg 
      aaggcacta ttgttgtga gtcaataca gtgaagcca 
      gttctttgg gtgatatcg tcgatgctt tgaattcgt 
      cggttgata ataacgagg aagggattg tagttaaag 
      acaccaaat cggtttaca aatgcagcg accggaaca 
      gcggttggt ttaaggtca aaatggaat acttcgacg 
      atgttgttc acgatttgg acgtgattt tgatggggg 
      gttgttaca gttcgggca aactcaatt ctttttttg 
      ttggggtca gttcagggg ctaacacct acttgtcct 
      ttgggagaa tctcgtccg gtttgagtg acgagcagc 
      tcgaccttt tggccgaaa aatttcagt cgaaagggg 
      ttgatttca agtcatttt caaccgaga gcgagggca 
      agctccagt tcggccgtg accgccctg acctctaca 
      tcgagcccc acaattcgt gcattttgc aaatccgag 
      ccactgaat taatccgga ccacgaatg acacggtca 
      agtgtccct acactttac gttttcccc gtgttttga 
      aaatccggg acgacaaac cggtcgatg agtgcttct 
      caataaacg agctcctcg aactcgcgg gccagaaca 
      agcccgtta tcaaactga acaaaagac acatcgaac 
      tctctgaaa taagcgcca aggcaagac cggcgaaaa 
      aaatcaagc ttgaggtca tcaagtcgg atttgttga 
      ccgaatccg gggattttt taaccggaa agcgctttt 
      acgtcgatt ccgggaccc agaagtggg ggcttgatg 
      acatctacc acgcgatca agaaagccg ggggtgagg 
      taagctacc gcgtggaac cgaacgttg atgtgattt 
      tggtgtcga aagttggta aaaaagtgg ccgagctga 
      tgaaacaac ccaatcatt ttgatatag tccacgttg 
      attggctgc accgcgtta tggaatacc gggagttgg 
      tggactaca agccggggg agatgttct acaaagggg 
      ctaattttc ggagagacg ttggctcag attcggata 
      gatttggcg attcgttcc gtgaggaag cgacgaaag 
      agtcgctaa agtgtgcgg tacgggtga tgcaagaag 
      cgggcgatt ttttgaata caaacgggg cggtttttt 
      gcggcgata agccgagtt ttggtgatt atgtcgcgt 
      attttgatt gtttcgaag agattaata atccgcgtt 
      ccaaacgca tttattatt cgcttccag atgaagcgg 
      agtttgagt tttatagtg gtactgtgg ttaaagaga 
      taacggcgc aagttaatt tgataataa ttgcggaaa 
      ataacgagg atcgtgtct caattgtta gtgattttt 
      tggcaaatg aaggactcg ggacggttg atattgtgt 
      cgaaagatt tcttgtaca gtcgcattt ctagtcaaa 
      attaagtct ttcagtgat ttttattgt attttcttt 
      gtatatttc gtaattgaa taaactttt actaataga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY