Status
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Gene information
General information
TC012207 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4458343..4459585
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012207
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01947

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012207 RA
        MKFLLLAAF VAVCTASTV YRNRNISVN DDGSILITS 
        PTGKRVMIT RPVGVNGQR NIDISVSGP NIPTKTIQI 
        NDNNLYDSE YAYGGYPYS SYDGVNEEY RTKRGSKGK 
        SQGDVLGEI FREYQGAVN EPQYEVLLT RISKAVKKG 
        ELSPQVFDL LEGLERGYG SGYGYGSGY GYGSGYGYG 
        SGYGSGYGS KYGSGYGSG YGVGYGYGS GYGPYASGY 
        YGSGYGPYY GGYYGSYLP WWYPRSYYR GQQYGPYYG 
        NYYGKGQYY GSSRYVNPL LQKMLYGSQ GGYGDYYYN 
        QGGYGYVPY KSYAKGYLG KNYYYPYRG VSDSYETYG 
        QLKEEQPIE DFVEFEREQ EYPYAGFYG YYYNSPYYK 
        DYRGGRGSY YNSVPQVQA Y
      • >TC012207 RB
        MITRPVGVN GQRNIDISV SGPNIPTKT IQINDNNLY 
        DSEYAYGGY PYSSYDGVN EEYRTKRGS KGKSQGDVL 
        GEIFREYQG AVNEPQYEV LLTRISKAV KKGELSPQV 
        FDLLEGLER GYGSGYGYG SGYGYGSGY GYGSGYGSG 
        YGSKYGSGY GSGYGVGYG YGSGYGPYA SGYYGSGYG 
        PYYGGYYGS YLPWWYPRS YYRGQQYGP YYGNYYGKG 
        QYYGSSRYV NPLLQKMLY GSQGGYGDY YYNQGGYGY 
        VPYKSYAKG YLGKNYYYP YRGVSDSYE TYGQLKEEQ 
        PIEDFVEFE REQEYPYAG FYGYYYNSP YYKDYRGGR 
        GSYYNSVPQ VQAY
      CDS sequence
      • >TC012207 RA
        atgaagttc cttcttctg gccgccttc gtcgccgtg 
        tgcactgct tccacagtg tacaggaac cgcaacatc 
        tccgtcaac gacgatggc agcatcttg atcacctct 
        ccaaccggt aagcgtgtc atgatcacc cgccctgtg 
        ggcgtcaac ggccagcga aacatcgac atctccgtc 
        tccgggccc aacatccca accaagacc atccaaatc 
        aacgacaac aacctctac gactccgag tacgcctac 
        ggcggctac ccgtactct tcctacgac ggcgtcaac 
        gaggagtac aggaccaag aggggctcc aagggcaag 
        agccaaggg gacgtcctc ggggagatc ttcagggag 
        taccaaggc gccgtcaac gagccccag tacgaagtc 
        ctcctgacc agaatcagc aaggctgtg aagaagggc 
        gagctcagt ccccaggtg tttgatttg ctggaaggg 
        ctcgagcgc ggatacggc tctggatat ggctacggg 
        tctgggtat gggtacgga tctgggtat gggtacgga 
        tctgggtat ggctcagga tatgggtct aaatatggg 
        tctggatat gggtctgga tatggcgtt gggtatggt 
        tatggatct ggctatggc ccatatgcg tctgggtat 
        tatggttct ggttacgga ccgtactat ggagggtac 
        tatggctcc tacctgcca tggtggtac ccccgcagc 
        tactaccgc ggccaacag tacggacca tactatggc 
        aactactat ggcaaaggc cagtactat ggcagctct 
        cgctatgtg aaccccctt ttgcaaaaa atgttgtac 
        ggcagccaa ggcggctac ggtgactac tactacaac 
        caaggtggc tacggatac gtcccttac aaatcgtac 
        gcgaaaggg tatcttggc aaaaactac tactacccc 
        taccgtgga gtttccgat tcttatgaa acttatgga 
        cagttgaaa gaagaacaa cccattgag gatttcgtt 
        gaattcgaa cgagaacaa gaataccca tatgctggc 
        ttctacggg tattactac aactcaccc tactacaag 
        gactaccgt gggggaaga ggctcttac tacaacagt 
        gttcctcaa gttcaggcc tattaa
      • >TC012207 RB
        atgatcacc cgccctgtg ggcgtcaac ggccagcga 
        aacatcgac atctccgtc tccgggccc aacatccca 
        accaagacc atccaaatc aacgacaac aacctctac 
        gactccgag tacgcctac ggcggctac ccgtactct 
        tcctacgac ggcgtcaac gaggagtac aggaccaag 
        aggggctcc aagggcaag agccaaggg gacgtcctc 
        ggggagatc ttcagggag taccaaggc gccgtcaac 
        gagccccag tacgaagtc ctcctgacc agaatcagc 
        aaggctgtg aagaagggc gagctcagt ccccaggtg 
        tttgatttg ctggaaggg ctcgagcgc ggatacggc 
        tctggatat ggctacggg tctgggtat gggtacgga 
        tctgggtat gggtacgga tctgggtat ggctcagga 
        tatgggtct aaatatggg tctggatat gggtctgga 
        tatggcgtt gggtatggt tatggatct ggctatggc 
        ccatatgcg tctgggtat tatggttct ggttacgga 
        ccgtactat ggagggtac tatggctcc tacctgcca 
        tggtggtac ccccgcagc tactaccgc ggccaacag 
        tacggacca tactatggc aactactat ggcaaaggc 
        cagtactat ggcagctct cgctatgtg aaccccctt 
        ttgcaaaaa atgttgtac ggcagccaa ggcggctac 
        ggtgactac tactacaac caaggtggc tacggatac 
        gtcccttac aaatcgtac gcgaaaggg tatcttggc 
        aaaaactac tactacccc taccgtgga gtttccgat 
        tcttatgaa acttatgga cagttgaaa gaagaacaa 
        cccattgag gatttcgtt gaattcgaa cgagaacaa 
        gaataccca tatgctggc ttctacggg tattactac 
        aactcaccc tactacaag gactaccgt gggggaaga 
        ggctcttac tacaacagt gttcctcaa gttcaggcc 
        tattaa
      mRNA sequence
      • >TC012207 RA
        atcagatcc aaaccagca acccgctca agatgaagt 
        tccttcttc tggccgcct tcgtcgccg tgtgcactg 
        cttccacag tgtacagga accgcaaca tctccgtca 
        acgacgatg gcagcatct tgatcacct ctccaaccg 
        gtaagcgtg tcatgatca cccgccctg tgggcgtca 
        acggccagc gaaacatcg acatctccg tctccgggc 
        ccaacatcc caaccaaga ccatccaaa tcaacgaca 
        acaacctct acgactccg agtacgcct acggcggct 
        acccgtact cttcctacg acggcgtca acgaggagt 
        acaggacca agaggggct ccaagggca agagccaag 
        gggacgtcc tcggggaga tcttcaggg agtaccaag 
        gcgccgtca acgagcccc agtacgaag tcctcctga 
        ccagaatca gcaaggctg tgaagaagg gcgagctca 
        gtccccagg tgtttgatt tgctggaag ggctcgagc 
        gcggatacg gctctggat atggctacg ggtctgggt 
        atgggtacg gatctgggt atgggtacg gatctgggt 
        atggctcag gatatgggt ctaaatatg ggtctggat 
        atgggtctg gatatggcg ttgggtatg gttatggat 
        ctggctatg gcccatatg cgtctgggt attatggtt 
        ctggttacg gaccgtact atggagggt actatggct 
        cctacctgc catggtggt acccccgca gctactacc 
        gcggccaac agtacggac catactatg gcaactact 
        atggcaaag gccagtact atggcagct ctcgctatg 
        tgaaccccc ttttgcaaa aaatgttgt acggcagcc 
        aaggcggct acggtgact actactaca accaaggtg 
        gctacggat acgtccctt acaaatcgt acgcgaaag 
        ggtatcttg gcaaaaact actactacc cctaccgtg 
        gagtttccg attcttatg aaacttatg gacagttga 
        aagaagaac aacccattg aggatttcg ttgaattcg 
        aacgagaac aagaatacc catatgctg gcttctacg 
        ggtattact acaactcac cctactaca aggactacc 
        gtgggggaa gaggctctt actacaaca gtgttcctc 
        aagttcagg cctattaag ctgatatga aaatgaatg 
        ttttgaaac ggcaattgt aattatgtg cattgtgaa 
        ataaatatt gatttatta c
      • >TC012207 RB
        acagtgtac aggaaccgc aacatctcc gtcaacgac 
        gatggcagc atcttgatc acctctcca accggtaag 
        cgtgtcatg atcacccgc cctgtgggc gtcaacggc 
        cagcgaaac atcgacatc tccgtctcc gggcccaac 
        atcccaacc aagaccatc caaatcaac gacaacaac 
        ctctacgac tccgagtac gcctacggc ggctacccg 
        tactcttcc tacgacggc gtcaacgag gagtacagg 
        accaagagg ggctccaag ggcaagagc caaggggac 
        gtcctcggg gagatcttc agggagtac caaggcgcc 
        gtcaacgag ccccagtac gaagtcctc ctgaccaga 
        atcagcaag gctgtgaag aagggcgag ctcagtccc 
        caggtgttt gatttgctg gaagggctc gagcgcgga 
        tacggctct ggatatggc tacgggtct gggtatggg 
        tacggatct gggtatggg tacggatct gggtatggc 
        tcaggatat gggtctaaa tatgggtct ggatatggg 
        tctggatat ggcgttggg tatggttat ggatctggc 
        tatggccca tatgcgtct gggtattat ggttctggt 
        tacggaccg tactatgga gggtactat ggctcctac 
        ctgccatgg tggtacccc cgcagctac taccgcggc 
        caacagtac ggaccatac tatggcaac tactatggc 
        aaaggccag tactatggc agctctcgc tatgtgaac 
        ccccttttg caaaaaatg ttgtacggc agccaaggc 
        ggctacggt gactactac tacaaccaa ggtggctac 
        ggatacgtc ccttacaaa tcgtacgcg aaagggtat 
        cttggcaaa aactactac tacccctac cgtggagtt 
        tccgattct tatgaaact tatggacag ttgaaagaa 
        gaacaaccc attgaggat ttcgttgaa ttcgaacga 
        gaacaagaa tacccatat gctggcttc tacgggtat 
        tactacaac tcaccctac tacaaggac taccgtggg 
        ggaagaggc tcttactac aacagtgtt cctcaagtt 
        caggcctat taagctgat atgaaaatg aatgttttg 
        aaacggcaa ttgtaatta tgtgcattg tgaaataaa 
        tattgattt attac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY