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Gene information
General information
TC012193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3531788..3552226
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC012193
      iBeetle screen iB_01946

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012193 RA
        MTTEDISKK LQNLNLEKN QDINILLLG ETGVGKSTF 
INSIANYLH FKSFKKANK AEELLVLIP SLITVTNEN 
FEQVTLTIG SDKNEFQEV GAAGTQDVK TYVFPIGGG 
KTRLRLIDS PGMGDPRGI EQDDINCEN ILAYLSQLS 
ELHAICFLM KPTTTRKTI LFEYCVKQI LSRLEKSAS 
KNIIFIFTN TRGTNYTPG DTLPCLKTV VDDIKTKPP 
HVDIPLKGN IFSVDNEAF RFLVAMKNN IKYDEELQQ 
RFIDSWNKS AEECWKLIS YIVGDAAHD PLKPHQLSN 
TISINEARR IITQLSQPL ADISQLIYD NIRILERHR 
RNLAMENQS LDELKKKLY IPTVKLEVT QLTQPVTVC 
TTPKCADVV KVGDLKKWH YRQRCHDPC YLTNVTKEF 
VGDLNLMHC AAMMGRMCK KCTCDFSVH MHVYYLTKT 
IDDKVVDSV VKDNINQKE QALREGQRL IGEIDRRKR 
ELEEEREAI IKSTAQFAH FLQHNAIAP FNDSYKGYI 
EYLISREKS LGRSGDPEV VHHLEQLLQ RYSELKSAF 
DRSLELSQQ HGLNTVISS AVTAQSVMN TIHQLYKLK 
HNGKKIHEL LKKQKASRN SEYDKSCEY VHPKSSDLS 
KKSKPKKKD GSKSTDTEG NKKEEKKKK ASKTDDESK 
NKYKQYNQN YNSNQRSNN QDHQQRPNF SSYQRREPP 
PPYPGYNSG PYQGQHFPN PRKDHDDNL IFKVEVSHP 
NAGTKQQRT INPDYAQTP NHDQVYDGH RHYQGPPHG 
PPYYGHGPG EYHGPPPHH QGYYPPPHG DYGPPQGGP 
YGAYGPPPG PYHPPAGPY HPPPGPYHP PPQPSYGGP 
QGPYQGGYY GPKPGQYQR NYQPGRGRQ HFNKNKQGG 
HRNQSKGRN SQGKKSGNE KSSRSDTDS SD
      CDS sequence
      • >TC012193 RA
        atgaccact gaggacatt tctaagaaa ctacaaaac 
ctcaatttg gagaaaaac caagacatc aacatctta 
ctacttggc gaaacaggc gtcggaaaa tcaacattt 
attaactcc atcgccaat tatttgcac tttaaaagc 
ttcaagaaa gccaacaaa gccgaagaa ttgctcgtc 
ctaatcccc tcacttatc acagtcact aacgaaaat 
ttcgaacaa gtcactctt accatcggc tctgataaa 
aatgaattc caagaagtg ggcgcagca ggaacccaa 
gacgtcaaa acttacgtt ttcccaatc gggggagga 
aaaacgcgc cttcgcttg atcgattct cccggaatg 
ggtgatccc agaggcatc gaacaagac gacatcaac 
tgcgaaaat atcttggct tatttgtcc caactgagc 
gaactccac gccatatgt tttctaatg aagccgaca 
acaacccga aaaactatc ttgtttgaa tattgcgtc 
aagcagatt ttgtcacga ttggagaaa tcggccagc 
aagaacatc atctttatt tttaccaat accagaggc 
acgaattat acacctgga gacactttg ccctgcttg 
aaaactgtc gttgatgac attaagacc aaaccgcca 
catgttgat atccctctg aaaggtaat atcttttcg 
gtggacaac gaggcgttt agattcctc gtggctatg 
aaaaacaac atcaaatac gatgaagag cttcaacag 
cggtttatc gatagttgg aataaatcg gcagaggag 
tgttggaag cttatttcg tacattgtt ggagatgcg 
gcacacgat ccattgaaa cctcatcaa ctgtcgaac 
actatcagc attaatgaa gccaggaga attattact 
cagttgtcg cagcctttg gctgatatc tcccaactg 
atttacgac aacatccgc attttggag cgtcacaga 
cgcaatttg gcaatggag aatcagagt ttggatgag 
ttaaagaag aaattgtac atccccact gttaagctt 
gaagttact caattgacg caacctgta acagtgtgt 
acaactccc aaatgtgct gatgttgtg aaggtcgga 
gatctaaag aaatggcac taccggcag agatgtcac 
gacccttgc tatctaacc aacgtgaca aaagagttt 
gtgggtgat ttaaatttg atgcattgt gccgcaatg 
atgggaaga atgtgtaag aagtgcact tgcgatttt 
tcggtgcat atgcatgtg tactacttg acgaaaacc 
atagatgac aaggttgtt gatagcgtc gtgaaagat 
aatattaat cagaaggaa caagcactc cgtgaaggt 
cagagatta attggagaa atagatcga cgaaagcga 
gaactggaa gaagaacga gaagccatt atcaaaagt 
acggcacag tttgcccat tttcttcaa cacaacgca 
attgcacca tttaacgat tcttacaaa ggttacatt 
gaatatcta atatcacgt gagaagagt cttggtcga 
agtggagat ccagaagtg gttcaccat ttggaacaa 
ttgttgcag cggtatagt gaactaaaa tcagctttt 
gaccgaagc cttgaactg agtcaacaa catggcctc 
aacacagtg atttcgtct gcagtcaca gctcaaagc 
gttatgaac acaattcac caactttat aagttaaaa 
cataacggt aaaaagatc catgagttg ttaaagaaa 
caaaaagct tcaaggaac tccgagtac gataaaagc 
tgcgagtac gtgcatcca aaatcatca gacttaagc 
aagaaaagt aaaccaaag aagaaagat ggtagcaaa 
agcaccgac actgaaggg aataaaaag gaagagaag 
aagaagaag gcaagcaaa actgacgat gaatcgaaa 
aataaatac aaacaatac aaccaaaac tacaattcg 
aatcaaaga tcgaacaat caagaccac caacagcgt 
ccaaatttt tcgtcttat caaaggagg gaacccccg 
ccaccatat cctggttac aattcgggt ccttaccaa 
gggcaacat tttccaaac ccacgcaaa gatcatgac 
gacaatctt atcttcaaa gttgaagta agccatccg 
aatgctggt actaagcaa caaagaacc ataaacccc 
gattatgcg caaacacca aatcatgac caggtttac 
gatggtcat cggcattac caaggacca ccccatggg 
cctccctac tatggtcat gggccgggg gagtatcac 
ggaccccca ccacatcat caagggtat tatcctcca 
cctcatgga gattatggc ccaccccaa ggaggaccg 
tatggtgcc tatggtcct cctcctggc ccatatcat 
cctcctgct ggcccatat catcctcct cctggccca 
tatcatcct ccaccgcag ccttcatat ggtggaccc 
caagggccc tatcaaggg gggtactac gggccaaaa 
ccaggccag tatcaaagg aactatcaa ccagggcgt 
ggtcgccaa cattttaat aaaaataag caaggggga 
catcgaaac caatcaaaa ggaagaaac agtcaaggg 
aagaagagt ggaaatgag aagagtagt cgttctgat 
acagattcg agcgattaa
      mRNA sequence
      • >TC012193 RA
        aactcatat gcaaccaac aaatcacgt cagttgtag 
aattgtaga atttaacta aaattaagt acaaaaaat 
agtattttt cgtgttaaa gactttgtt tagttctat 
ttaatgcgc actgagaac caaagttgt tagtaagat 
aataagaga ttgataata ttgaggttg ttgatttgt 
tacaatttt cggggaagt ccggttgta tgtgcatcg 
cgttcatta ttacgtcgg tggtacgat tatagatct 
caaggacga ataccgttg aatacgttg cagtttacc 
atttccaag ttagtagtg tttaatttt tttccgttc 
aacaagtgc atttggaat tttagattg gatatttta 
taatagtaa tgacccacc tttagtcta agatgacca 
ctgaggaca tttctaaga aactacaaa acctcaatt 
tggagaaaa accaagaca tcaacatct tactacttg 
gcgaaacag gcgtcggaa aatcaacat ttattaact 
ccatcgcca attatttgc actttaaaa gcttcaaga 
aagccaaca aagccgaag aattgctcg tcctaatcc 
cctcactta tcacagtca ctaacgaaa atttcgaac 
aagtcactc ttaccatcg gctctgata aaaatgaat 
tccaagaag tgggcgcag caggaaccc aagacgtca 
aaacttacg ttttcccaa tcgggggag gaaaaacgc 
gccttcgct tgatcgatt ctcccggaa tgggtgatc 
ccagaggca tcgaacaag acgacatca actgcgaaa 
atatcttgg cttatttgt cccaactga gcgaactcc 
acgccatat gttttctaa tgaagccga caacaaccc 
gaaaaacta tcttgtttg aatattgcg tcaagcaga 
ttttgtcac gattggaga aatcggcca gcaagaaca 
tcatcttta tttttacca ataccagag gcacgaatt 
atacacctg gagacactt tgccctgct tgaaaactg 
tcgttgatg acattaaga ccaaaccgc cacatgttg 
atatccctc tgaaaggta atatctttt cggtggaca 
acgaggcgt ttagattcc tcgtggcta tgaaaaaca 
acatcaaat acgatgaag agcttcaac agcggttta 
tcgatagtt ggaataaat cggcagagg agtgttgga 
agcttattt cgtacattg ttggagatg cggcacacg 
atccattga aacctcatc aactgtcga acactatca 
gcattaatg aagccagga gaattatta ctcagttgt 
cgcagcctt tggctgata tctcccaac tgatttacg 
acaacatcc gcattttgg agcgtcaca gacgcaatt 
tggcaatgg agaatcaga gtttggatg agttaaaga 
agaaattgt acatcccca ctgttaagc ttgaagtta 
ctcaattga cgcaacctg taacagtgt gtacaactc 
ccaaatgtg ctgatgttg tgaaggtcg gagatctaa 
agaaatggc actaccggc agagatgtc acgaccctt 
gctatctaa ccaacgtga caaaagagt ttgtgggtg 
atttaaatt tgatgcatt gtgccgcaa tgatgggaa 
gaatgtgta agaagtgca cttgcgatt tttcggtgc 
atatgcatg tgtactact tgacgaaaa ccatagatg 
acaaggttg ttgatagcg tcgtgaaag ataatatta 
atcagaagg aacaagcac tccgtgaag gtcagagat 
taattggag aaatagatc gacgaaagc gagaactgg 
aagaagaac gagaagcca ttatcaaaa gtacggcac 
agtttgccc attttcttc aacacaacg caattgcac 
catttaacg attcttaca aaggttaca ttgaatatc 
taatatcac gtgagaaga gtcttggtc gaagtggag 
atccagaag tggttcacc atttggaac aattgttgc 
agcggtata gtgaactaa aatcagctt ttgaccgaa 
gccttgaac tgagtcaac aacatggcc tcaacacag 
tgatttcgt ctgcagtca cagctcaaa gcgttatga 
acacaattc accaacttt ataagttaa aacataacg 
gtaaaaaga tccatgagt tgttaaaga aacaaaaag 
cttcaagga actccgagt acgataaaa gctgcgagt 
acgtgcatc caaaatcat cagacttaa gcaagaaaa 
gtaaaccaa agaagaaag atggtagca aaagcaccg 
acactgaag ggaataaaa aggaagaga agaagaaga 
aggcaagca aaactgacg atgaatcga aaaataaat 
acaaacaat acaaccaaa actacaatt cgaatcaaa 
gatcgaaca atcaagacc accaacagc gtccaaatt 
tttcgtctt atcaaagga gggaacccc cgccaccat 
atcctggtt acaattcgg gtccttacc aagggcaac 
attttccaa acccacgca aagatcatg acgacaatc 
ttatcttca aagttgaag taagccatc cgaatgctg 
gtactaagc aacaaagaa ccataaacc ccgattatg 
cgcaaacac caaatcatg accaggttt acgatggtc 
atcggcatt accaaggac caccccatg ggcctccct 
actatggtc atgggccgg gggagtatc acggacccc 
caccacatc atcaagggt attatcctc cacctcatg 
gagattatg gcccacccc aaggaggac cgtatggtg 
cctatggtc ctcctcctg gcccatatc atcctcctg 
ctggcccat atcatcctc ctcctggcc catatcatc 
ctccaccgc agccttcat atggtggac cccaagggc 
cctatcaag gggggtact acgggccaa aaccaggcc 
agtatcaaa ggaactatc aaccagggc gtggtcgcc 
aacatttta ataaaaata agcaagggg gacatcgaa 
accaatcaa aaggaagaa acagtcaag ggaagaaga 
gtggaaatg agaagagta gtcgttctg atacagatt 
cgagcgatt aaaacggtg ggatgtttt cgttatgca 
agggttaag tttttagtg taatcgtgt tagttatat 
tttctactg ggagtatgt attggtttg tcataaatc 
ttatcttat gaattgata aagtaaact gtattcttt 
agaggagtt tcaaataaa attaaatga gcatt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY