| General information | |||
| TC012193 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 3531788..3552226 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC012193 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01946 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC012193 RA
MTTEDISKK LQNLNLEKN QDINILLLG ETGVGKSTF INSIANYLH FKSFKKANK AEELLVLIP SLITVTNEN FEQVTLTIG SDKNEFQEV GAAGTQDVK TYVFPIGGG KTRLRLIDS PGMGDPRGI EQDDINCEN ILAYLSQLS ELHAICFLM KPTTTRKTI LFEYCVKQI LSRLEKSAS KNIIFIFTN TRGTNYTPG DTLPCLKTV VDDIKTKPP HVDIPLKGN IFSVDNEAF RFLVAMKNN IKYDEELQQ RFIDSWNKS AEECWKLIS YIVGDAAHD PLKPHQLSN TISINEARR IITQLSQPL ADISQLIYD NIRILERHR RNLAMENQS LDELKKKLY IPTVKLEVT QLTQPVTVC TTPKCADVV KVGDLKKWH YRQRCHDPC YLTNVTKEF VGDLNLMHC AAMMGRMCK KCTCDFSVH MHVYYLTKT IDDKVVDSV VKDNINQKE QALREGQRL IGEIDRRKR ELEEEREAI IKSTAQFAH FLQHNAIAP FNDSYKGYI EYLISREKS LGRSGDPEV VHHLEQLLQ RYSELKSAF DRSLELSQQ HGLNTVISS AVTAQSVMN TIHQLYKLK HNGKKIHEL LKKQKASRN SEYDKSCEY VHPKSSDLS KKSKPKKKD GSKSTDTEG NKKEEKKKK ASKTDDESK NKYKQYNQN YNSNQRSNN QDHQQRPNF SSYQRREPP PPYPGYNSG PYQGQHFPN PRKDHDDNL IFKVEVSHP NAGTKQQRT INPDYAQTP NHDQVYDGH RHYQGPPHG PPYYGHGPG EYHGPPPHH QGYYPPPHG DYGPPQGGP YGAYGPPPG PYHPPAGPY HPPPGPYHP PPQPSYGGP QGPYQGGYY GPKPGQYQR NYQPGRGRQ HFNKNKQGG HRNQSKGRN SQGKKSGNE KSSRSDTDS SD
- >TC012193 RA
atgaccact gaggacatt tctaagaaa ctacaaaac ctcaatttg gagaaaaac caagacatc aacatctta ctacttggc gaaacaggc gtcggaaaa tcaacattt attaactcc atcgccaat tatttgcac tttaaaagc ttcaagaaa gccaacaaa gccgaagaa ttgctcgtc ctaatcccc tcacttatc acagtcact aacgaaaat ttcgaacaa gtcactctt accatcggc tctgataaa aatgaattc caagaagtg ggcgcagca ggaacccaa gacgtcaaa acttacgtt ttcccaatc gggggagga aaaacgcgc cttcgcttg atcgattct cccggaatg ggtgatccc agaggcatc gaacaagac gacatcaac tgcgaaaat atcttggct tatttgtcc caactgagc gaactccac gccatatgt tttctaatg aagccgaca acaacccga aaaactatc ttgtttgaa tattgcgtc aagcagatt ttgtcacga ttggagaaa tcggccagc aagaacatc atctttatt tttaccaat accagaggc acgaattat acacctgga gacactttg ccctgcttg aaaactgtc gttgatgac attaagacc aaaccgcca catgttgat atccctctg aaaggtaat atcttttcg gtggacaac gaggcgttt agattcctc gtggctatg aaaaacaac atcaaatac gatgaagag cttcaacag cggtttatc gatagttgg aataaatcg gcagaggag tgttggaag cttatttcg tacattgtt ggagatgcg gcacacgat ccattgaaa cctcatcaa ctgtcgaac actatcagc attaatgaa gccaggaga attattact cagttgtcg cagcctttg gctgatatc tcccaactg atttacgac aacatccgc attttggag cgtcacaga cgcaatttg gcaatggag aatcagagt ttggatgag ttaaagaag aaattgtac atccccact gttaagctt gaagttact caattgacg caacctgta acagtgtgt acaactccc aaatgtgct gatgttgtg aaggtcgga gatctaaag aaatggcac taccggcag agatgtcac gacccttgc tatctaacc aacgtgaca aaagagttt gtgggtgat ttaaatttg atgcattgt gccgcaatg atgggaaga atgtgtaag aagtgcact tgcgatttt tcggtgcat atgcatgtg tactacttg acgaaaacc atagatgac aaggttgtt gatagcgtc gtgaaagat aatattaat cagaaggaa caagcactc cgtgaaggt cagagatta attggagaa atagatcga cgaaagcga gaactggaa gaagaacga gaagccatt atcaaaagt acggcacag tttgcccat tttcttcaa cacaacgca attgcacca tttaacgat tcttacaaa ggttacatt gaatatcta atatcacgt gagaagagt cttggtcga agtggagat ccagaagtg gttcaccat ttggaacaa ttgttgcag cggtatagt gaactaaaa tcagctttt gaccgaagc cttgaactg agtcaacaa catggcctc aacacagtg atttcgtct gcagtcaca gctcaaagc gttatgaac acaattcac caactttat aagttaaaa cataacggt aaaaagatc catgagttg ttaaagaaa caaaaagct tcaaggaac tccgagtac gataaaagc tgcgagtac gtgcatcca aaatcatca gacttaagc aagaaaagt aaaccaaag aagaaagat ggtagcaaa agcaccgac actgaaggg aataaaaag gaagagaag aagaagaag gcaagcaaa actgacgat gaatcgaaa aataaatac aaacaatac aaccaaaac tacaattcg aatcaaaga tcgaacaat caagaccac caacagcgt ccaaatttt tcgtcttat caaaggagg gaacccccg ccaccatat cctggttac aattcgggt ccttaccaa gggcaacat tttccaaac ccacgcaaa gatcatgac gacaatctt atcttcaaa gttgaagta agccatccg aatgctggt actaagcaa caaagaacc ataaacccc gattatgcg caaacacca aatcatgac caggtttac gatggtcat cggcattac caaggacca ccccatggg cctccctac tatggtcat gggccgggg gagtatcac ggaccccca ccacatcat caagggtat tatcctcca cctcatgga gattatggc ccaccccaa ggaggaccg tatggtgcc tatggtcct cctcctggc ccatatcat cctcctgct ggcccatat catcctcct cctggccca tatcatcct ccaccgcag ccttcatat ggtggaccc caagggccc tatcaaggg gggtactac gggccaaaa ccaggccag tatcaaagg aactatcaa ccagggcgt ggtcgccaa cattttaat aaaaataag caaggggga catcgaaac caatcaaaa ggaagaaac agtcaaggg aagaagagt ggaaatgag aagagtagt cgttctgat acagattcg agcgattaa
- >TC012193 RA
aactcatat gcaaccaac aaatcacgt cagttgtag aattgtaga atttaacta aaattaagt acaaaaaat agtattttt cgtgttaaa gactttgtt tagttctat ttaatgcgc actgagaac caaagttgt tagtaagat aataagaga ttgataata ttgaggttg ttgatttgt tacaatttt cggggaagt ccggttgta tgtgcatcg cgttcatta ttacgtcgg tggtacgat tatagatct caaggacga ataccgttg aatacgttg cagtttacc atttccaag ttagtagtg tttaatttt tttccgttc aacaagtgc atttggaat tttagattg gatatttta taatagtaa tgacccacc tttagtcta agatgacca ctgaggaca tttctaaga aactacaaa acctcaatt tggagaaaa accaagaca tcaacatct tactacttg gcgaaacag gcgtcggaa aatcaacat ttattaact ccatcgcca attatttgc actttaaaa gcttcaaga aagccaaca aagccgaag aattgctcg tcctaatcc cctcactta tcacagtca ctaacgaaa atttcgaac aagtcactc ttaccatcg gctctgata aaaatgaat tccaagaag tgggcgcag caggaaccc aagacgtca aaacttacg ttttcccaa tcgggggag gaaaaacgc gccttcgct tgatcgatt ctcccggaa tgggtgatc ccagaggca tcgaacaag acgacatca actgcgaaa atatcttgg cttatttgt cccaactga gcgaactcc acgccatat gttttctaa tgaagccga caacaaccc gaaaaacta tcttgtttg aatattgcg tcaagcaga ttttgtcac gattggaga aatcggcca gcaagaaca tcatcttta tttttacca ataccagag gcacgaatt atacacctg gagacactt tgccctgct tgaaaactg tcgttgatg acattaaga ccaaaccgc cacatgttg atatccctc tgaaaggta atatctttt cggtggaca acgaggcgt ttagattcc tcgtggcta tgaaaaaca acatcaaat acgatgaag agcttcaac agcggttta tcgatagtt ggaataaat cggcagagg agtgttgga agcttattt cgtacattg ttggagatg cggcacacg atccattga aacctcatc aactgtcga acactatca gcattaatg aagccagga gaattatta ctcagttgt cgcagcctt tggctgata tctcccaac tgatttacg acaacatcc gcattttgg agcgtcaca gacgcaatt tggcaatgg agaatcaga gtttggatg agttaaaga agaaattgt acatcccca ctgttaagc ttgaagtta ctcaattga cgcaacctg taacagtgt gtacaactc ccaaatgtg ctgatgttg tgaaggtcg gagatctaa agaaatggc actaccggc agagatgtc acgaccctt gctatctaa ccaacgtga caaaagagt ttgtgggtg atttaaatt tgatgcatt gtgccgcaa tgatgggaa gaatgtgta agaagtgca cttgcgatt tttcggtgc atatgcatg tgtactact tgacgaaaa ccatagatg acaaggttg ttgatagcg tcgtgaaag ataatatta atcagaagg aacaagcac tccgtgaag gtcagagat taattggag aaatagatc gacgaaagc gagaactgg aagaagaac gagaagcca ttatcaaaa gtacggcac agtttgccc attttcttc aacacaacg caattgcac catttaacg attcttaca aaggttaca ttgaatatc taatatcac gtgagaaga gtcttggtc gaagtggag atccagaag tggttcacc atttggaac aattgttgc agcggtata gtgaactaa aatcagctt ttgaccgaa gccttgaac tgagtcaac aacatggcc tcaacacag tgatttcgt ctgcagtca cagctcaaa gcgttatga acacaattc accaacttt ataagttaa aacataacg gtaaaaaga tccatgagt tgttaaaga aacaaaaag cttcaagga actccgagt acgataaaa gctgcgagt acgtgcatc caaaatcat cagacttaa gcaagaaaa gtaaaccaa agaagaaag atggtagca aaagcaccg acactgaag ggaataaaa aggaagaga agaagaaga aggcaagca aaactgacg atgaatcga aaaataaat acaaacaat acaaccaaa actacaatt cgaatcaaa gatcgaaca atcaagacc accaacagc gtccaaatt tttcgtctt atcaaagga gggaacccc cgccaccat atcctggtt acaattcgg gtccttacc aagggcaac attttccaa acccacgca aagatcatg acgacaatc ttatcttca aagttgaag taagccatc cgaatgctg gtactaagc aacaaagaa ccataaacc ccgattatg cgcaaacac caaatcatg accaggttt acgatggtc atcggcatt accaaggac caccccatg ggcctccct actatggtc atgggccgg gggagtatc acggacccc caccacatc atcaagggt attatcctc cacctcatg gagattatg gcccacccc aaggaggac cgtatggtg cctatggtc ctcctcctg gcccatatc atcctcctg ctggcccat atcatcctc ctcctggcc catatcatc ctccaccgc agccttcat atggtggac cccaagggc cctatcaag gggggtact acgggccaa aaccaggcc agtatcaaa ggaactatc aaccagggc gtggtcgcc aacatttta ataaaaata agcaagggg gacatcgaa accaatcaa aaggaagaa acagtcaag ggaagaaga gtggaaatg agaagagta gtcgttctg atacagatt cgagcgatt aaaacggtg ggatgtttt cgttatgca agggttaag tttttagtg taatcgtgt tagttatat tttctactg ggagtatgt attggtttg tcataaatc ttatcttat gaattgata aagtaaact gtattcttt agaggagtt tcaaataaa attaaatga gcatt
