Status
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Gene information
General information
TC012193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3531788..3552226
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012193
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_01946

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012193 RA
        MTTEDISKK LQNLNLEKN QDINILLLG ETGVGKSTF 
        INSIANYLH FKSFKKANK AEELLVLIP SLITVTNEN 
        FEQVTLTIG SDKNEFQEV GAAGTQDVK TYVFPIGGG 
        KTRLRLIDS PGMGDPRGI EQDDINCEN ILAYLSQLS 
        ELHAICFLM KPTTTRKTI LFEYCVKQI LSRLEKSAS 
        KNIIFIFTN TRGTNYTPG DTLPCLKTV VDDIKTKPP 
        HVDIPLKGN IFSVDNEAF RFLVAMKNN IKYDEELQQ 
        RFIDSWNKS AEECWKLIS YIVGDAAHD PLKPHQLSN 
        TISINEARR IITQLSQPL ADISQLIYD NIRILERHR 
        RNLAMENQS LDELKKKLY IPTVKLEVT QLTQPVTVC 
        TTPKCADVV KVGDLKKWH YRQRCHDPC YLTNVTKEF 
        VGDLNLMHC AAMMGRMCK KCTCDFSVH MHVYYLTKT 
        IDDKVVDSV VKDNINQKE QALREGQRL IGEIDRRKR 
        ELEEEREAI IKSTAQFAH FLQHNAIAP FNDSYKGYI 
        EYLISREKS LGRSGDPEV VHHLEQLLQ RYSELKSAF 
        DRSLELSQQ HGLNTVISS AVTAQSVMN TIHQLYKLK 
        HNGKKIHEL LKKQKASRN SEYDKSCEY VHPKSSDLS 
        KKSKPKKKD GSKSTDTEG NKKEEKKKK ASKTDDESK 
        NKYKQYNQN YNSNQRSNN QDHQQRPNF SSYQRREPP 
        PPYPGYNSG PYQGQHFPN PRKDHDDNL IFKVEVSHP 
        NAGTKQQRT INPDYAQTP NHDQVYDGH RHYQGPPHG 
        PPYYGHGPG EYHGPPPHH QGYYPPPHG DYGPPQGGP 
        YGAYGPPPG PYHPPAGPY HPPPGPYHP PPQPSYGGP 
        QGPYQGGYY GPKPGQYQR NYQPGRGRQ HFNKNKQGG 
        HRNQSKGRN SQGKKSGNE KSSRSDTDS SD
      CDS sequence
      • >TC012193 RA
        atgaccact gaggacatt tctaagaaa ctacaaaac 
        ctcaatttg gagaaaaac caagacatc aacatctta 
        ctacttggc gaaacaggc gtcggaaaa tcaacattt 
        attaactcc atcgccaat tatttgcac tttaaaagc 
        ttcaagaaa gccaacaaa gccgaagaa ttgctcgtc 
        ctaatcccc tcacttatc acagtcact aacgaaaat 
        ttcgaacaa gtcactctt accatcggc tctgataaa 
        aatgaattc caagaagtg ggcgcagca ggaacccaa 
        gacgtcaaa acttacgtt ttcccaatc gggggagga 
        aaaacgcgc cttcgcttg atcgattct cccggaatg 
        ggtgatccc agaggcatc gaacaagac gacatcaac 
        tgcgaaaat atcttggct tatttgtcc caactgagc 
        gaactccac gccatatgt tttctaatg aagccgaca 
        acaacccga aaaactatc ttgtttgaa tattgcgtc 
        aagcagatt ttgtcacga ttggagaaa tcggccagc 
        aagaacatc atctttatt tttaccaat accagaggc 
        acgaattat acacctgga gacactttg ccctgcttg 
        aaaactgtc gttgatgac attaagacc aaaccgcca 
        catgttgat atccctctg aaaggtaat atcttttcg 
        gtggacaac gaggcgttt agattcctc gtggctatg 
        aaaaacaac atcaaatac gatgaagag cttcaacag 
        cggtttatc gatagttgg aataaatcg gcagaggag 
        tgttggaag cttatttcg tacattgtt ggagatgcg 
        gcacacgat ccattgaaa cctcatcaa ctgtcgaac 
        actatcagc attaatgaa gccaggaga attattact 
        cagttgtcg cagcctttg gctgatatc tcccaactg 
        atttacgac aacatccgc attttggag cgtcacaga 
        cgcaatttg gcaatggag aatcagagt ttggatgag 
        ttaaagaag aaattgtac atccccact gttaagctt 
        gaagttact caattgacg caacctgta acagtgtgt 
        acaactccc aaatgtgct gatgttgtg aaggtcgga 
        gatctaaag aaatggcac taccggcag agatgtcac 
        gacccttgc tatctaacc aacgtgaca aaagagttt 
        gtgggtgat ttaaatttg atgcattgt gccgcaatg 
        atgggaaga atgtgtaag aagtgcact tgcgatttt 
        tcggtgcat atgcatgtg tactacttg acgaaaacc 
        atagatgac aaggttgtt gatagcgtc gtgaaagat 
        aatattaat cagaaggaa caagcactc cgtgaaggt 
        cagagatta attggagaa atagatcga cgaaagcga 
        gaactggaa gaagaacga gaagccatt atcaaaagt 
        acggcacag tttgcccat tttcttcaa cacaacgca 
        attgcacca tttaacgat tcttacaaa ggttacatt 
        gaatatcta atatcacgt gagaagagt cttggtcga 
        agtggagat ccagaagtg gttcaccat ttggaacaa 
        ttgttgcag cggtatagt gaactaaaa tcagctttt 
        gaccgaagc cttgaactg agtcaacaa catggcctc 
        aacacagtg atttcgtct gcagtcaca gctcaaagc 
        gttatgaac acaattcac caactttat aagttaaaa 
        cataacggt aaaaagatc catgagttg ttaaagaaa 
        caaaaagct tcaaggaac tccgagtac gataaaagc 
        tgcgagtac gtgcatcca aaatcatca gacttaagc 
        aagaaaagt aaaccaaag aagaaagat ggtagcaaa 
        agcaccgac actgaaggg aataaaaag gaagagaag 
        aagaagaag gcaagcaaa actgacgat gaatcgaaa 
        aataaatac aaacaatac aaccaaaac tacaattcg 
        aatcaaaga tcgaacaat caagaccac caacagcgt 
        ccaaatttt tcgtcttat caaaggagg gaacccccg 
        ccaccatat cctggttac aattcgggt ccttaccaa 
        gggcaacat tttccaaac ccacgcaaa gatcatgac 
        gacaatctt atcttcaaa gttgaagta agccatccg 
        aatgctggt actaagcaa caaagaacc ataaacccc 
        gattatgcg caaacacca aatcatgac caggtttac 
        gatggtcat cggcattac caaggacca ccccatggg 
        cctccctac tatggtcat gggccgggg gagtatcac 
        ggaccccca ccacatcat caagggtat tatcctcca 
        cctcatgga gattatggc ccaccccaa ggaggaccg 
        tatggtgcc tatggtcct cctcctggc ccatatcat 
        cctcctgct ggcccatat catcctcct cctggccca 
        tatcatcct ccaccgcag ccttcatat ggtggaccc 
        caagggccc tatcaaggg gggtactac gggccaaaa 
        ccaggccag tatcaaagg aactatcaa ccagggcgt 
        ggtcgccaa cattttaat aaaaataag caaggggga 
        catcgaaac caatcaaaa ggaagaaac agtcaaggg 
        aagaagagt ggaaatgag aagagtagt cgttctgat 
        acagattcg agcgattaa
      mRNA sequence
      • >TC012193 RA
        aactcatat gcaaccaac aaatcacgt cagttgtag 
        aattgtaga atttaacta aaattaagt acaaaaaat 
        agtattttt cgtgttaaa gactttgtt tagttctat 
        ttaatgcgc actgagaac caaagttgt tagtaagat 
        aataagaga ttgataata ttgaggttg ttgatttgt 
        tacaatttt cggggaagt ccggttgta tgtgcatcg 
        cgttcatta ttacgtcgg tggtacgat tatagatct 
        caaggacga ataccgttg aatacgttg cagtttacc 
        atttccaag ttagtagtg tttaatttt tttccgttc 
        aacaagtgc atttggaat tttagattg gatatttta 
        taatagtaa tgacccacc tttagtcta agatgacca 
        ctgaggaca tttctaaga aactacaaa acctcaatt 
        tggagaaaa accaagaca tcaacatct tactacttg 
        gcgaaacag gcgtcggaa aatcaacat ttattaact 
        ccatcgcca attatttgc actttaaaa gcttcaaga 
        aagccaaca aagccgaag aattgctcg tcctaatcc 
        cctcactta tcacagtca ctaacgaaa atttcgaac 
        aagtcactc ttaccatcg gctctgata aaaatgaat 
        tccaagaag tgggcgcag caggaaccc aagacgtca 
        aaacttacg ttttcccaa tcgggggag gaaaaacgc 
        gccttcgct tgatcgatt ctcccggaa tgggtgatc 
        ccagaggca tcgaacaag acgacatca actgcgaaa 
        atatcttgg cttatttgt cccaactga gcgaactcc 
        acgccatat gttttctaa tgaagccga caacaaccc 
        gaaaaacta tcttgtttg aatattgcg tcaagcaga 
        ttttgtcac gattggaga aatcggcca gcaagaaca 
        tcatcttta tttttacca ataccagag gcacgaatt 
        atacacctg gagacactt tgccctgct tgaaaactg 
        tcgttgatg acattaaga ccaaaccgc cacatgttg 
        atatccctc tgaaaggta atatctttt cggtggaca 
        acgaggcgt ttagattcc tcgtggcta tgaaaaaca 
        acatcaaat acgatgaag agcttcaac agcggttta 
        tcgatagtt ggaataaat cggcagagg agtgttgga 
        agcttattt cgtacattg ttggagatg cggcacacg 
        atccattga aacctcatc aactgtcga acactatca 
        gcattaatg aagccagga gaattatta ctcagttgt 
        cgcagcctt tggctgata tctcccaac tgatttacg 
        acaacatcc gcattttgg agcgtcaca gacgcaatt 
        tggcaatgg agaatcaga gtttggatg agttaaaga 
        agaaattgt acatcccca ctgttaagc ttgaagtta 
        ctcaattga cgcaacctg taacagtgt gtacaactc 
        ccaaatgtg ctgatgttg tgaaggtcg gagatctaa 
        agaaatggc actaccggc agagatgtc acgaccctt 
        gctatctaa ccaacgtga caaaagagt ttgtgggtg 
        atttaaatt tgatgcatt gtgccgcaa tgatgggaa 
        gaatgtgta agaagtgca cttgcgatt tttcggtgc 
        atatgcatg tgtactact tgacgaaaa ccatagatg 
        acaaggttg ttgatagcg tcgtgaaag ataatatta 
        atcagaagg aacaagcac tccgtgaag gtcagagat 
        taattggag aaatagatc gacgaaagc gagaactgg 
        aagaagaac gagaagcca ttatcaaaa gtacggcac 
        agtttgccc attttcttc aacacaacg caattgcac 
        catttaacg attcttaca aaggttaca ttgaatatc 
        taatatcac gtgagaaga gtcttggtc gaagtggag 
        atccagaag tggttcacc atttggaac aattgttgc 
        agcggtata gtgaactaa aatcagctt ttgaccgaa 
        gccttgaac tgagtcaac aacatggcc tcaacacag 
        tgatttcgt ctgcagtca cagctcaaa gcgttatga 
        acacaattc accaacttt ataagttaa aacataacg 
        gtaaaaaga tccatgagt tgttaaaga aacaaaaag 
        cttcaagga actccgagt acgataaaa gctgcgagt 
        acgtgcatc caaaatcat cagacttaa gcaagaaaa 
        gtaaaccaa agaagaaag atggtagca aaagcaccg 
        acactgaag ggaataaaa aggaagaga agaagaaga 
        aggcaagca aaactgacg atgaatcga aaaataaat 
        acaaacaat acaaccaaa actacaatt cgaatcaaa 
        gatcgaaca atcaagacc accaacagc gtccaaatt 
        tttcgtctt atcaaagga gggaacccc cgccaccat 
        atcctggtt acaattcgg gtccttacc aagggcaac 
        attttccaa acccacgca aagatcatg acgacaatc 
        ttatcttca aagttgaag taagccatc cgaatgctg 
        gtactaagc aacaaagaa ccataaacc ccgattatg 
        cgcaaacac caaatcatg accaggttt acgatggtc 
        atcggcatt accaaggac caccccatg ggcctccct 
        actatggtc atgggccgg gggagtatc acggacccc 
        caccacatc atcaagggt attatcctc cacctcatg 
        gagattatg gcccacccc aaggaggac cgtatggtg 
        cctatggtc ctcctcctg gcccatatc atcctcctg 
        ctggcccat atcatcctc ctcctggcc catatcatc 
        ctccaccgc agccttcat atggtggac cccaagggc 
        cctatcaag gggggtact acgggccaa aaccaggcc 
        agtatcaaa ggaactatc aaccagggc gtggtcgcc 
        aacatttta ataaaaata agcaagggg gacatcgaa 
        accaatcaa aaggaagaa acagtcaag ggaagaaga 
        gtggaaatg agaagagta gtcgttctg atacagatt 
        cgagcgatt aaaacggtg ggatgtttt cgttatgca 
        agggttaag tttttagtg taatcgtgt tagttatat 
        tttctactg ggagtatgt attggtttg tcataaatc 
        ttatcttat gaattgata aagtaaact gtattcttt 
        agaggagtt tcaaataaa attaaatga gcatt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY