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Gene information
General information
TC012192 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3597428..3606526
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012192
    iBeetle screen iB_01945

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber complete not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber shape irregular - Number of animals showing the phenotype: 4
    vitellogenic egg chamber potentially degenerated - Number of animals showing the phenotype: 4
    tropharium potentially irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012192 RA
      MADSIELRM SVTSIKRVD PYVKDILAT ATHVALYKF 
      NTSTNEWEK TDTEGALFI YSRNGEPFH SIMVMNRLN 
      TNNVIEPIV KDFDYQMQI PFLLYRNSK SKIFGIWFF 
      NREECVHIT YLIETVMES LKDKSNERS KKQENGVDI 
      FSLLSKAQE DFNKTPTKQ ESVPPAFST PRDCTSKSV 
      MDFFAKAST KSTHQTPIV TGGDGENVL QRLMSNPAH 
      TVEHIEKQQ RSITPQEQV PLRHKINAD RRSVPISFP 
      STSNTVDRK DQENRMSFM GVSPPNQAY QGSSPLAFL 
      LQGPSQLQQ PEESDAMRT SPFAQFLDN AQKPQLMTP 
      MMFTTPTNT EKLDNSKTE PKIENIDLL TEKQLAQAL 
      IHLLKTNSN FTRQIHEAY VNSIMERVN CNSNSLM
    CDS sequence
    • >TC012192 RA
      atggccgat tctatcgaa ttgcgtatg agtgttacg 
      tctatcaaa agagttgat ccatatgtg aaagatatt 
      ttagcgact gctacacac gttgcttta tacaaattc 
      aacacctcg accaacgaa tgggagaaa accgacact 
      gaaggcgct ttgtttatc tacagcaga aatggagag 
      ccgtttcac agtattatg gttatgaac cgactcaac 
      acaaacaat gtgatagaa ccaattgtg aaagacttt 
      gactatcag atgcaaata ccgtttctg ctctacagg 
      aattcaaaa agcaaaata tttggcatc tggttcttc 
      aaccgtgaa gagtgtgtg cacattacc tatttaatt 
      gaaacagtg atggagagt ctcaaagac aagtcgaac 
      gagaggtcg aagaagcag gagaacggc gttgacatt 
      ttcagtctt ctctcgaag gcgcaggaa gacttcaat 
      aaaacgccg acgaagcaa gaatcggtt ccgccggcg 
      ttttctaca cctagggat tgtacttca aaaagtgtt 
      atggacttt tttgctaaa gccagcacc aagtcaaca 
      catcaaacg cccattgtg acggggggc gacggagag 
      aacgtgtta cagaggctg atgtccaac ccggctcac 
      acggttgaa cacattgag aagcagcag aggtctatc 
      actccacag gagcaggta cctttacgc cacaaaata 
      aacgcagac aggcgcagc gttccgatc tcattcccc 
      agcactagc aacacagtt gaccgaaaa gaccaagaa 
      aatcgaatg agttttatg ggggtttca ccccccaac 
      caggcttat caaggttca tcgcctttg gcattcctt 
      ctgcaaggg ccaagtcaa ttgcaacaa cctgaagaa 
      tctgatgca atgaggact tcgcccttt gcacaattc 
      ctagacaac gcgcagaaa ccgcaactg atgactccg 
      atgatgttc acaactccg acaaacaca gagaagttg 
      gacaacagc aagaccgaa cctaaaatc gaaaatatc 
      gatctcttg acggaaaag cagctagca caagctctt 
      attcatttg ttgaaaact aattcgaat tttaccaga 
      cagatacac gaagcgtat gttaattcg atcatggaa 
      agggtgaat tgtaacagt aattcgctt atgtaa
    mRNA sequence
    • >TC012192 RA
      tttacttgg actgtggta ctgagtttg tttcgagga 
      aactgtgta gttcttcag ttggggacg gttaaattt 
      tgcgaaaag tagatgttg tggttaccg gccgcggtc 
      tgcattacc ggcattttt gcagtttca aatcatggc 
      cgattctat cgaattgcg tatgagtgt tacgtctat 
      caaaagagt tgatccata tgtgaaaga tattttagc 
      gactgctac acacgttgc tttatacaa attcaacac 
      ctcgaccaa cgaatggga gaaaaccga cactgaagg 
      cgctttgtt tatctacag cagaaatgg agagccgtt 
      tcacagtat tatggttat gaaccgact caacacaaa 
      caatgtgat agaaccaat tgtgaaaga ctttgacta 
      tcagatgca aataccgtt tctgctcta caggaattc 
      aaaaagcaa aatatttgg catctggtt cttcaaccg 
      tgaagagtg tgtgcacat tacctattt aattgaaac 
      agtgatgga gagtctcaa agacaagtc gaacgagag 
      gtcgaagaa gcaggagaa cggcgttga cattttcag 
      tcttctctc gaaggcgca ggaagactt caataaaac 
      gccgacgaa gcaagaatc ggttccgcc ggcgttttc 
      tacacctag ggattgtac ttcaaaaag tgttatgga 
      cttttttgc taaagccag caccaagtc aacacatca 
      aacgcccat tgtgacggg gggcgacgg agagaacgt 
      gttacagag gctgatgtc caacccggc tcacacggt 
      tgaacacat tgagaagca gcagaggtc tatcactcc 
      acaggagca ggtaccttt acgccacaa aataaacgc 
      agacaggcg cagcgttcc gatctcatt ccccagcac 
      tagcaacac agttgaccg aaaagacca agaaaatcg 
      aatgagttt tatgggggt ttcaccccc caaccaggc 
      ttatcaagg ttcatcgcc tttggcatt ccttctgca 
      agggccaag tcaattgca acaacctga agaatctga 
      tgcaatgag gacttcgcc ctttgcaca attcctaga 
      caacgcgca gaaaccgca actgatgac tccgatgat 
      gttcacaac tccgacaaa cacagagaa gttggacaa 
      cagcaagac cgaacctaa aatcgaaaa tatcgatct 
      cttgacgga aaagcagct agcacaagc tcttattca 
      tttgttgaa aactaattc gaattttac cagacagat 
      acacgaagc gtatgttaa ttcgatcat ggaaagggt 
      gaattgtaa cagtaattc gcttatgta agtttgatg 
      ttttttaag ttggacaca gtcttgata gagtcaagg 
      ctatcccga atgccttaa attattaga ttttaataa 
      cgacattct ttcctcgtt gtttgactg aagaagggt 
      tttaatacg taagtaaaa gttcgattt caaacaaca 
      cattgtgtc gtgcaatga tgtcttcta taacctaac 
      taaatcgct ggtatttag acttcagta tcaaaaaga 
      agtaaactt agaacttgt tgtaacgta tatacagtt 
      acatgattg tgatacata gaaatacat gtttaggat 
      ttgaaaatt caataactt tgctattaa tatagttta 
      gacgattaa acgtgcatg tagttggaa tgaactgtt 
      tggttttgt tttttattt tcttttact tctttctag 
      tcatttatc tgttttaat atccgaaga ggaaatcat 
      tttgtttca tgtcgtcaa taaatagcg cccgattaa 
      taatataca atgttctca gttaagccc actcaaaga 
      actatattt gtagcaaaa cgtatttca atctcatag 
      atgtatatt tttaagcaa aagtatata aattcacgc 
      tgtaatgtt gttgtcaac agaattgtc atctttgtt 
      gatttatat ttttttact tgaatgaat ctgtactac 
      cacttccca gtttccagg tcatttgaa cttgtctca 
      ctttgttga gtaaaacct ttttttaaa taaagacgt 
      tacatctga tttagaact tttatttgc tggatataa 
      aaaggcaca ataaataag cattttctt aactaatat 
      tatacaaaa taagcgaat acagtcaga atgttaaaa 
      gtatacaag atatgtaca tattacgag taattaaat 
      ttgagcagt cattatcaa gtggtaaac atcacggca 
      atcctggaa atattttat ggtgcggtc agtcgcaaa 
      atccaagaa tgtcacagc cgcaagtgc gttaagtct 
      tatctctgg aggactaaa ctaggacac aaacaattt 
      aagttgttt ggaaatcat gttaaactg tatggttaa 
      aagggaaaa taaataaaa tgatttttt acaactaca 
      ggatgatta aaaaaaaac agcagcgcg ttatagatt 
      ttttatatg atccgattt caaaaatta aaaaagtaa 
      aatagttgg cttaaaaaa atgtaaatg ttagtatcc 
      agggtgtta gggaatggc ttagcaata attcgtttg 
      tgaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY