Status
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Gene information
General information
TC012191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3609424..3642046
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012191
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01944

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     labrum position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum potentially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012191 RA
      MMHPSSKNE PRTAKISCI YDTPCDPKA TDYEKLYCP 
NNELSEPPR TRCDRLKTG SSCEALKHA VASLHRLDD 
FHKEKIGSG FFSEVFKVT HRVTGQVMV LKMNLLRSN 
RRNMLKEVQ LMNKLSHPN ILNLMGVCV HEGQLHALT 
EYINGGSLE QLIQNRNID LPQPTRVSL ARDISYGMS 
YLHSKDVFH RDLTSKNVL IKRLESGEL QAVVGDFGL 
ASSFPDGKT KLCTVGSPY WMSPECLKG QYYDQQSDV 
FSYGIVLCE LIARVEADP DQLPRTDNF GLDYLAFTE 
MCGQNVVPE FLNLAFRCC TIDPKSRPT FNEIVQILT 
EILADLQHH TSDRGEQQA KLASCGAKS EEQLPVLLV 
QSSAPQHRK IVHRRSLSE DVSMLSLTM HATPSEKAR 
RHALTMCRQ DPHYKPRTS NPFAALTQF RGVKKFLGN 
FSSCCELPS PFIESTESP KSLPGSPTA SRKAATTSS 
SRSPFFVHP FFKGGSSTN LLELGTTEG PNITLRRRG 
SCESGFYSS VGECLSPSS LWDSGTAVS SLRSLDELE 
PAELQALCK RASSICTDS SEDISSLTG SEWTQDLKP 
KNISYMVDY FERKGASLR HPGGVRGGL QLSSRIAAL 
RKSLEKQGA AGAVNSNSL VTPQRHKYN RIVVCEGAV 
RSKLPLFDK K
    CDS sequence
    • >TC012191 RA
      atgatgcac ccgagcagc aaaaatgag ccccgtacc 
gctaaaatt tcgtgcatt tacgacacc ccctgcgac 
cccaaagcg accgactac gagaagctg tactgcccc 
aataacgag ctgtcggag cccccgagg acccgctgt 
gatagactc aaaactgga tcgtcctgc gaggccctg 
aagcatgcc gtggcatcc ctccataga ctcgacgac 
ttccacaag gaaaaaatc ggatcgggg tttttctcg 
gaggttttc aaggtgacc caccgcgta accggtcaa 
gtgatggtg ttgaagatg aacttgttg cgttcgaat 
cgccgtaac atgttgaag gaggtccaa ttgatgaat 
aaattatcc catccgaat atcctaaat ttgatgggt 
gtatgcgtg cacgaaggt caattacat gccctcacc 
gaatacatc aatggtggt tcattagag caactcata 
caaaataga aacatcgat ttaccacaa ccaacaagg 
gtatccttg gcaagggat atatcatac ggcatgagc 
tatcttcat tccaaggac gtatttcac agggactta 
acaagtaaa aatgtccta ataaagcgc ctggaatcg 
ggcgagctg caggccgtg gtgggcgac ttcggcttg 
gcgtcatcg ttccctgac ggcaaaacg aagctgtgt 
accgtcgga tccccttat tggatgtcc cccgagtgc 
ctgaaaggt caatactac gaccaacaa agtgatgta 
tttagttac ggaatagtg ttatgcgaa ctgatagca 
cgtgtcgaa gctgatccc gatcagttg cccagaact 
gacaatttc ggtctcgat tatttagcg tttaccgaa 
atgtgtgga cagaacgtt gtacctgaa tttttaaat 
ttggcattt cgatgctgc actatcgat ccaaaaagt 
cggccgaca ttcaacgaa atcgttcaa atattaacc 
gaaatcttg gccgatcta caacatcat acatccgat 
cgaggagaa caacaagcc aagttggca agttgtggc 
gccaaaagc gaggaacaa ctgcctgta cttttggta 
caatcgtcg gcaccacaa cacaggaaa attgtccac 
cggcgatcg ttatctgaa gacgtatcc atgttgtcg 
ctaacgatg cacgcgaca cctagcgag aaagcccgt 
cgtcacgcc ttgacgatg tgccgtcaa gacccgcac 
tacaagcct cgtacgagc aatccattc gcggcttta 
acccaattc cgcggtgtt aaaaagttc ctcggcaat 
ttttcgtct tgttgcgaa ttgccatct cccttcatc 
gagtccaca gaatcgccc aaatcgctc cccggctct 
ccaaccgcc tcccggaaa gcagcgaca acgtcgtcg 
tctcgttcg cccttcttc gtgcatccc tttttcaaa 
gggggctcc agcacgaac ctcctggag ttgggaacc 
accgaaggc cccaacatc acgttgcga cgtcggggc 
tcctgtgaa tcggggttt tattcaagc gtgggggaa 
tgtctctct ccgagtagt ttatgggac agtgggaca 
gcggttagt tcgttgcgc tcgcttgat gagttggaa 
ccggccgaa ttgcaggcg ttatgtaag agagcgtcg 
tcaatttgc acagatagc agcgaagat atttcgagt 
ctgacgggc tccgagtgg acgcaggac ttgaaaccg 
aagaatatc tcctacatg gtggactac ttcgagcgg 
aagggggcc agtctgagg cacccgggg ggcgttcga 
gggggctta cagctcagt tcgaggatt gcagcgctg 
cgcaaatcg ctggagaag cagggggct gccggtgct 
gttaattcg aattcgctt gtgacgcca cagaggcac 
aagtacaac cggattgtt gtctgtgaa ggtgcagtt 
aggtcaaag ttgcccctt tttgataaa aagtag
    mRNA sequence
    • >TC012191 RA
      aactattta tctgtaatt tcggttcac tttcgtaac 
aacgataaa tactaaatc gtatttaga atgaaaatt 
agttatttg cggtgaagc aagaatttg ttccctaaa 
gttttggaa gtccctcgt atggtggaa gtttcctta 
ggtgctgcc ctctataag gattgctta gagaattct 
gctttaccg gtcattttg gtggctgtc agttttaac 
ctcacttaa ccgtatttt atgtaaggc cgaaattta 
attcgtaca aagtgattg actgatttt tacctttaa 
tctagcaaa atgaccaca aaaaatgta ctcgggact 
ttgacccaa attagattt ttgagctgc cattcagcc 
agcatcaca agaccccac agattaact tacaagagg 
acatacccc actgttctt gttaaccca gatgggtca 
actatcaat attaaatac cacgaaccc aggcaaatc 
attaaactg cctttgaat atttggact ctcagtgag 
gctgaaaga aaggcacga ttggaggca agaaaacca 
aagaagaaa gttaaattt gaggatgat cttgatgat 
agtttcgac gcaaataaa tatgtaaaa tacttgaaa 
aagaaatag attaggtaa cattatgta gcgaattgt 
ctcaataaa gtttaataa ccgtttagt attgttttg 
aaacagtta attgttgga aatttaaaa tattttgtt 
aattaaatc gactgaatc ggccacttt cattatggt 
aatttagct attgtagaa acaattgta tgagtatgg 
agaagaaag tatgtgaat tacaatttc ctacatggg 
aacatttca cttttttga aaacgtttt aaaactgtc 
cattcgcga ttcacaacg ttgtttaat aacaatttc 
gtaatcgat tcattggct ttgggaacg gaacacgtt 
tgaaagtgg cgcttcaac ccaaccgcg catgcgtcc 
gttttgttg ctcaacgtg ccatcgcgc cgtcattgt 
ttagcccgg cgggaggaa ggagacgca gatatttgc 
ggatgaagt tttccacaa catggcagc tatgtatac 
tagatgaac gggtgaaag cttgcactg tcattacaa 
actttttat ttacgtgtg aaacttcaa attgctagc 
tcagctctt gatttcagt gcctaaaag tgaggtgtc 
ttgtgtcga acgaggtgt ctgaatggt gtgcgaagt 
gtactattt atgtcaagc tgtagcagt gcacacaat 
ttctaaatt taattataa aacctgcat aaatccatt 
gcgcgttat atttaccca gaaaagtga tgtcaaatg 
agctttcgg ccgttttga ccgtcggct gcaatgtcg 
tgctccgtt tccagtgaa ccacaacat taaaattat 
gtgtgattt taagacttc aagagtggc ccgcctaag 
tcgtacaaa aacatgtac gatattggt taacatcac 
ataggaccc cattctgtt atgatgcac ccgagcagc 
aaaaatgag ccccgtacc gctaaaatt tcgtgcatt 
tacgacacc ccctgcgac cccaaagcg accgactac 
gagaagctg tactgcccc aataacgag ctgtcggag 
cccccgagg acccgctgt gatagactc aaaactgga 
tcgtcctgc gaggccctg aagcatgcc gtggcatcc 
ctccataga ctcgacgac ttccacaag gaaaaaatc 
ggatcgggg tttttctcg gaggttttc aaggtgacc 
caccgcgta accggtcaa gtgatggtg ttgaagatg 
aacttgttg cgttcgaat cgccgtaac atgttgaag 
gaggtccaa ttgatgaat aaattatcc catccgaat 
atcctaaat ttgatgggt gtatgcgtg cacgaaggt 
caattacat gccctcacc gaatacatc aatggtggt 
tcattagag caactcata caaaataga aacatcgat 
ttaccacaa ccaacaagg gtatccttg gcaagggat 
atatcatac ggcatgagc tatcttcat tccaaggac 
gtatttcac agggactta acaagtaaa aatgtccta 
ataaagcgc ctggaatcg ggcgagctg caggccgtg 
gtgggcgac ttcggcttg gcgtcatcg ttccctgac 
ggcaaaacg aagctgtgt accgtcgga tccccttat 
tggatgtcc cccgagtgc ctgaaaggt caatactac 
gaccaacaa agtgatgta tttagttac ggaatagtg 
ttatgcgaa ctgatagca cgtgtcgaa gctgatccc 
gatcagttg cccagaact gacaatttc ggtctcgat 
tatttagcg tttaccgaa atgtgtgga cagaacgtt 
gtacctgaa tttttaaat ttggcattt cgatgctgc 
actatcgat ccaaaaagt cggccgaca ttcaacgaa 
atcgttcaa atattaacc gaaatcttg gccgatcta 
caacatcat acatccgat cgaggagaa caacaagcc 
aagttggca agttgtggc gccaaaagc gaggaacaa 
ctgcctgta cttttggta caatcgtcg gcaccacaa 
cacaggaaa attgtccac cggcgatcg ttatctgaa 
gacgtatcc atgttgtcg ctaacgatg cacgcgaca 
cctagcgag aaagcccgt cgtcacgcc ttgacgatg 
tgccgtcaa gacccgcac tacaagcct cgtacgagc 
aatccattc gcggcttta acccaattc cgcggtgtt 
aaaaagttc ctcggcaat ttttcgtct tgttgcgaa 
ttgccatct cccttcatc gagtccaca gaatcgccc 
aaatcgctc cccggctct ccaaccgcc tcccggaaa 
gcagcgaca acgtcgtcg tctcgttcg cccttcttc 
gtgcatccc tttttcaaa gggggctcc agcacgaac 
ctcctggag ttgggaacc accgaaggc cccaacatc 
acgttgcga cgtcggggc tcctgtgaa tcggggttt 
tattcaagc gtgggggaa tgtctctct ccgagtagt 
ttatgggac agtgggaca gcggttagt tcgttgcgc 
tcgcttgat gagttggaa ccggccgaa ttgcaggcg 
ttatgtaag agagcgtcg tcaatttgc acagatagc 
agcgaagat atttcgagt ctgacgggc tccgagtgg 
acgcaggac ttgaaaccg aagaatatc tcctacatg 
gtggactac ttcgagcgg aagggggcc agtctgagg 
cacccgggg ggcgttcga gggggctta cagctcagt 
tcgaggatt gcagcgctg cgcaaatcg ctggagaag 
cagggggct gccggtgct gttaattcg aattcgctt 
gtgacgcca cagaggcac aagtacaac cggattgtt 
gtctgtgaa ggtgcagtt aggtcaaag ttgcccctt 
tttgataaa aagtaggcc agtcttcct tcgttttgt 
ttcttctct agtcagatt gtaattaaa attgttaca 
tgatttgtg gcctaaaaa gtctatttt tcacgtcct 
tgttggttc acagttgta catatttgt gtgacctta 
aaattattg cgtttctag ttttggtaa atatgcggc 
ctatattag gtcggttta ttggagttg tgcgagaat 
taagcttcg tttttgcat ttatgttgt tttggacgt 
tttgaaaga attaagttc cgtttctgt ttcgatttt 
caaaatcgg ctaaatatt ttatgagtg tggaaaaag 
actgaaaaa tttacgatt ttcgtgtac taaatattt 
gctttgttt agctggttt tgatttgtg tgaaccaaa 
tgccaaatg tataaccga catatccaa gttttggtt 
gcaataatg taaaaatgt gatgaccgt ccatcagaa 
aagtattga agattatac caattatgt tactctttg 
tatttatga aatatttag tgagcaagt attactctt 
tttaacggt ctgtttgta tatcttcca tattgtctt 
gtcaataat atatagttg tttagtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY