General information | |||
TC012191 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 3609424..3642046 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012191 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01944 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
labrum position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
labrum potentially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head capsule not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012191 RA
MMHPSSKNE PRTAKISCI YDTPCDPKA TDYEKLYCP NNELSEPPR TRCDRLKTG SSCEALKHA VASLHRLDD FHKEKIGSG FFSEVFKVT HRVTGQVMV LKMNLLRSN RRNMLKEVQ LMNKLSHPN ILNLMGVCV HEGQLHALT EYINGGSLE QLIQNRNID LPQPTRVSL ARDISYGMS YLHSKDVFH RDLTSKNVL IKRLESGEL QAVVGDFGL ASSFPDGKT KLCTVGSPY WMSPECLKG QYYDQQSDV FSYGIVLCE LIARVEADP DQLPRTDNF GLDYLAFTE MCGQNVVPE FLNLAFRCC TIDPKSRPT FNEIVQILT EILADLQHH TSDRGEQQA KLASCGAKS EEQLPVLLV QSSAPQHRK IVHRRSLSE DVSMLSLTM HATPSEKAR RHALTMCRQ DPHYKPRTS NPFAALTQF RGVKKFLGN FSSCCELPS PFIESTESP KSLPGSPTA SRKAATTSS SRSPFFVHP FFKGGSSTN LLELGTTEG PNITLRRRG SCESGFYSS VGECLSPSS LWDSGTAVS SLRSLDELE PAELQALCK RASSICTDS SEDISSLTG SEWTQDLKP KNISYMVDY FERKGASLR HPGGVRGGL QLSSRIAAL RKSLEKQGA AGAVNSNSL VTPQRHKYN RIVVCEGAV RSKLPLFDK K
- >TC012191 RA
atgatgcac ccgagcagc aaaaatgag ccccgtacc gctaaaatt tcgtgcatt tacgacacc ccctgcgac cccaaagcg accgactac gagaagctg tactgcccc aataacgag ctgtcggag cccccgagg acccgctgt gatagactc aaaactgga tcgtcctgc gaggccctg aagcatgcc gtggcatcc ctccataga ctcgacgac ttccacaag gaaaaaatc ggatcgggg tttttctcg gaggttttc aaggtgacc caccgcgta accggtcaa gtgatggtg ttgaagatg aacttgttg cgttcgaat cgccgtaac atgttgaag gaggtccaa ttgatgaat aaattatcc catccgaat atcctaaat ttgatgggt gtatgcgtg cacgaaggt caattacat gccctcacc gaatacatc aatggtggt tcattagag caactcata caaaataga aacatcgat ttaccacaa ccaacaagg gtatccttg gcaagggat atatcatac ggcatgagc tatcttcat tccaaggac gtatttcac agggactta acaagtaaa aatgtccta ataaagcgc ctggaatcg ggcgagctg caggccgtg gtgggcgac ttcggcttg gcgtcatcg ttccctgac ggcaaaacg aagctgtgt accgtcgga tccccttat tggatgtcc cccgagtgc ctgaaaggt caatactac gaccaacaa agtgatgta tttagttac ggaatagtg ttatgcgaa ctgatagca cgtgtcgaa gctgatccc gatcagttg cccagaact gacaatttc ggtctcgat tatttagcg tttaccgaa atgtgtgga cagaacgtt gtacctgaa tttttaaat ttggcattt cgatgctgc actatcgat ccaaaaagt cggccgaca ttcaacgaa atcgttcaa atattaacc gaaatcttg gccgatcta caacatcat acatccgat cgaggagaa caacaagcc aagttggca agttgtggc gccaaaagc gaggaacaa ctgcctgta cttttggta caatcgtcg gcaccacaa cacaggaaa attgtccac cggcgatcg ttatctgaa gacgtatcc atgttgtcg ctaacgatg cacgcgaca cctagcgag aaagcccgt cgtcacgcc ttgacgatg tgccgtcaa gacccgcac tacaagcct cgtacgagc aatccattc gcggcttta acccaattc cgcggtgtt aaaaagttc ctcggcaat ttttcgtct tgttgcgaa ttgccatct cccttcatc gagtccaca gaatcgccc aaatcgctc cccggctct ccaaccgcc tcccggaaa gcagcgaca acgtcgtcg tctcgttcg cccttcttc gtgcatccc tttttcaaa gggggctcc agcacgaac ctcctggag ttgggaacc accgaaggc cccaacatc acgttgcga cgtcggggc tcctgtgaa tcggggttt tattcaagc gtgggggaa tgtctctct ccgagtagt ttatgggac agtgggaca gcggttagt tcgttgcgc tcgcttgat gagttggaa ccggccgaa ttgcaggcg ttatgtaag agagcgtcg tcaatttgc acagatagc agcgaagat atttcgagt ctgacgggc tccgagtgg acgcaggac ttgaaaccg aagaatatc tcctacatg gtggactac ttcgagcgg aagggggcc agtctgagg cacccgggg ggcgttcga gggggctta cagctcagt tcgaggatt gcagcgctg cgcaaatcg ctggagaag cagggggct gccggtgct gttaattcg aattcgctt gtgacgcca cagaggcac aagtacaac cggattgtt gtctgtgaa ggtgcagtt aggtcaaag ttgcccctt tttgataaa aagtag
- >TC012191 RA
aactattta tctgtaatt tcggttcac tttcgtaac aacgataaa tactaaatc gtatttaga atgaaaatt agttatttg cggtgaagc aagaatttg ttccctaaa gttttggaa gtccctcgt atggtggaa gtttcctta ggtgctgcc ctctataag gattgctta gagaattct gctttaccg gtcattttg gtggctgtc agttttaac ctcacttaa ccgtatttt atgtaaggc cgaaattta attcgtaca aagtgattg actgatttt tacctttaa tctagcaaa atgaccaca aaaaatgta ctcgggact ttgacccaa attagattt ttgagctgc cattcagcc agcatcaca agaccccac agattaact tacaagagg acatacccc actgttctt gttaaccca gatgggtca actatcaat attaaatac cacgaaccc aggcaaatc attaaactg cctttgaat atttggact ctcagtgag gctgaaaga aaggcacga ttggaggca agaaaacca aagaagaaa gttaaattt gaggatgat cttgatgat agtttcgac gcaaataaa tatgtaaaa tacttgaaa aagaaatag attaggtaa cattatgta gcgaattgt ctcaataaa gtttaataa ccgtttagt attgttttg aaacagtta attgttgga aatttaaaa tattttgtt aattaaatc gactgaatc ggccacttt cattatggt aatttagct attgtagaa acaattgta tgagtatgg agaagaaag tatgtgaat tacaatttc ctacatggg aacatttca cttttttga aaacgtttt aaaactgtc cattcgcga ttcacaacg ttgtttaat aacaatttc gtaatcgat tcattggct ttgggaacg gaacacgtt tgaaagtgg cgcttcaac ccaaccgcg catgcgtcc gttttgttg ctcaacgtg ccatcgcgc cgtcattgt ttagcccgg cgggaggaa ggagacgca gatatttgc ggatgaagt tttccacaa catggcagc tatgtatac tagatgaac gggtgaaag cttgcactg tcattacaa actttttat ttacgtgtg aaacttcaa attgctagc tcagctctt gatttcagt gcctaaaag tgaggtgtc ttgtgtcga acgaggtgt ctgaatggt gtgcgaagt gtactattt atgtcaagc tgtagcagt gcacacaat ttctaaatt taattataa aacctgcat aaatccatt gcgcgttat atttaccca gaaaagtga tgtcaaatg agctttcgg ccgttttga ccgtcggct gcaatgtcg tgctccgtt tccagtgaa ccacaacat taaaattat gtgtgattt taagacttc aagagtggc ccgcctaag tcgtacaaa aacatgtac gatattggt taacatcac ataggaccc cattctgtt atgatgcac ccgagcagc aaaaatgag ccccgtacc gctaaaatt tcgtgcatt tacgacacc ccctgcgac cccaaagcg accgactac gagaagctg tactgcccc aataacgag ctgtcggag cccccgagg acccgctgt gatagactc aaaactgga tcgtcctgc gaggccctg aagcatgcc gtggcatcc ctccataga ctcgacgac ttccacaag gaaaaaatc ggatcgggg tttttctcg gaggttttc aaggtgacc caccgcgta accggtcaa gtgatggtg ttgaagatg aacttgttg cgttcgaat cgccgtaac atgttgaag gaggtccaa ttgatgaat aaattatcc catccgaat atcctaaat ttgatgggt gtatgcgtg cacgaaggt caattacat gccctcacc gaatacatc aatggtggt tcattagag caactcata caaaataga aacatcgat ttaccacaa ccaacaagg gtatccttg gcaagggat atatcatac ggcatgagc tatcttcat tccaaggac gtatttcac agggactta acaagtaaa aatgtccta ataaagcgc ctggaatcg ggcgagctg caggccgtg gtgggcgac ttcggcttg gcgtcatcg ttccctgac ggcaaaacg aagctgtgt accgtcgga tccccttat tggatgtcc cccgagtgc ctgaaaggt caatactac gaccaacaa agtgatgta tttagttac ggaatagtg ttatgcgaa ctgatagca cgtgtcgaa gctgatccc gatcagttg cccagaact gacaatttc ggtctcgat tatttagcg tttaccgaa atgtgtgga cagaacgtt gtacctgaa tttttaaat ttggcattt cgatgctgc actatcgat ccaaaaagt cggccgaca ttcaacgaa atcgttcaa atattaacc gaaatcttg gccgatcta caacatcat acatccgat cgaggagaa caacaagcc aagttggca agttgtggc gccaaaagc gaggaacaa ctgcctgta cttttggta caatcgtcg gcaccacaa cacaggaaa attgtccac cggcgatcg ttatctgaa gacgtatcc atgttgtcg ctaacgatg cacgcgaca cctagcgag aaagcccgt cgtcacgcc ttgacgatg tgccgtcaa gacccgcac tacaagcct cgtacgagc aatccattc gcggcttta acccaattc cgcggtgtt aaaaagttc ctcggcaat ttttcgtct tgttgcgaa ttgccatct cccttcatc gagtccaca gaatcgccc aaatcgctc cccggctct ccaaccgcc tcccggaaa gcagcgaca acgtcgtcg tctcgttcg cccttcttc gtgcatccc tttttcaaa gggggctcc agcacgaac ctcctggag ttgggaacc accgaaggc cccaacatc acgttgcga cgtcggggc tcctgtgaa tcggggttt tattcaagc gtgggggaa tgtctctct ccgagtagt ttatgggac agtgggaca gcggttagt tcgttgcgc tcgcttgat gagttggaa ccggccgaa ttgcaggcg ttatgtaag agagcgtcg tcaatttgc acagatagc agcgaagat atttcgagt ctgacgggc tccgagtgg acgcaggac ttgaaaccg aagaatatc tcctacatg gtggactac ttcgagcgg aagggggcc agtctgagg cacccgggg ggcgttcga gggggctta cagctcagt tcgaggatt gcagcgctg cgcaaatcg ctggagaag cagggggct gccggtgct gttaattcg aattcgctt gtgacgcca cagaggcac aagtacaac cggattgtt gtctgtgaa ggtgcagtt aggtcaaag ttgcccctt tttgataaa aagtaggcc agtcttcct tcgttttgt ttcttctct agtcagatt gtaattaaa attgttaca tgatttgtg gcctaaaaa gtctatttt tcacgtcct tgttggttc acagttgta catatttgt gtgacctta aaattattg cgtttctag ttttggtaa atatgcggc ctatattag gtcggttta ttggagttg tgcgagaat taagcttcg tttttgcat ttatgttgt tttggacgt tttgaaaga attaagttc cgtttctgt ttcgatttt caaaatcgg ctaaatatt ttatgagtg tggaaaaag actgaaaaa tttacgatt ttcgtgtac taaatattt gctttgttt agctggttt tgatttgtg tgaaccaaa tgccaaatg tataaccga catatccaa gttttggtt gcaataatg taaaaatgt gatgaccgt ccatcagaa aagtattga agattatac caattatgt tactctttg tatttatga aatatttag tgagcaagt attactctt tttaacggt ctgtttgta tatcttcca tattgtctt gtcaataat atatagttg tttagtaa
