Status
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Gene information
General information
TC012191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3609424..3642046
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012191
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01944

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labrum position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum potentially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012191 RA
      MMHPSSKNE PRTAKISCI YDTPCDPKA TDYEKLYCP 
      NNELSEPPR TRCDRLKTG SSCEALKHA VASLHRLDD 
      FHKEKIGSG FFSEVFKVT HRVTGQVMV LKMNLLRSN 
      RRNMLKEVQ LMNKLSHPN ILNLMGVCV HEGQLHALT 
      EYINGGSLE QLIQNRNID LPQPTRVSL ARDISYGMS 
      YLHSKDVFH RDLTSKNVL IKRLESGEL QAVVGDFGL 
      ASSFPDGKT KLCTVGSPY WMSPECLKG QYYDQQSDV 
      FSYGIVLCE LIARVEADP DQLPRTDNF GLDYLAFTE 
      MCGQNVVPE FLNLAFRCC TIDPKSRPT FNEIVQILT 
      EILADLQHH TSDRGEQQA KLASCGAKS EEQLPVLLV 
      QSSAPQHRK IVHRRSLSE DVSMLSLTM HATPSEKAR 
      RHALTMCRQ DPHYKPRTS NPFAALTQF RGVKKFLGN 
      FSSCCELPS PFIESTESP KSLPGSPTA SRKAATTSS 
      SRSPFFVHP FFKGGSSTN LLELGTTEG PNITLRRRG 
      SCESGFYSS VGECLSPSS LWDSGTAVS SLRSLDELE 
      PAELQALCK RASSICTDS SEDISSLTG SEWTQDLKP 
      KNISYMVDY FERKGASLR HPGGVRGGL QLSSRIAAL 
      RKSLEKQGA AGAVNSNSL VTPQRHKYN RIVVCEGAV 
      RSKLPLFDK K
    CDS sequence
    • >TC012191 RA
      atgatgcac ccgagcagc aaaaatgag ccccgtacc 
      gctaaaatt tcgtgcatt tacgacacc ccctgcgac 
      cccaaagcg accgactac gagaagctg tactgcccc 
      aataacgag ctgtcggag cccccgagg acccgctgt 
      gatagactc aaaactgga tcgtcctgc gaggccctg 
      aagcatgcc gtggcatcc ctccataga ctcgacgac 
      ttccacaag gaaaaaatc ggatcgggg tttttctcg 
      gaggttttc aaggtgacc caccgcgta accggtcaa 
      gtgatggtg ttgaagatg aacttgttg cgttcgaat 
      cgccgtaac atgttgaag gaggtccaa ttgatgaat 
      aaattatcc catccgaat atcctaaat ttgatgggt 
      gtatgcgtg cacgaaggt caattacat gccctcacc 
      gaatacatc aatggtggt tcattagag caactcata 
      caaaataga aacatcgat ttaccacaa ccaacaagg 
      gtatccttg gcaagggat atatcatac ggcatgagc 
      tatcttcat tccaaggac gtatttcac agggactta 
      acaagtaaa aatgtccta ataaagcgc ctggaatcg 
      ggcgagctg caggccgtg gtgggcgac ttcggcttg 
      gcgtcatcg ttccctgac ggcaaaacg aagctgtgt 
      accgtcgga tccccttat tggatgtcc cccgagtgc 
      ctgaaaggt caatactac gaccaacaa agtgatgta 
      tttagttac ggaatagtg ttatgcgaa ctgatagca 
      cgtgtcgaa gctgatccc gatcagttg cccagaact 
      gacaatttc ggtctcgat tatttagcg tttaccgaa 
      atgtgtgga cagaacgtt gtacctgaa tttttaaat 
      ttggcattt cgatgctgc actatcgat ccaaaaagt 
      cggccgaca ttcaacgaa atcgttcaa atattaacc 
      gaaatcttg gccgatcta caacatcat acatccgat 
      cgaggagaa caacaagcc aagttggca agttgtggc 
      gccaaaagc gaggaacaa ctgcctgta cttttggta 
      caatcgtcg gcaccacaa cacaggaaa attgtccac 
      cggcgatcg ttatctgaa gacgtatcc atgttgtcg 
      ctaacgatg cacgcgaca cctagcgag aaagcccgt 
      cgtcacgcc ttgacgatg tgccgtcaa gacccgcac 
      tacaagcct cgtacgagc aatccattc gcggcttta 
      acccaattc cgcggtgtt aaaaagttc ctcggcaat 
      ttttcgtct tgttgcgaa ttgccatct cccttcatc 
      gagtccaca gaatcgccc aaatcgctc cccggctct 
      ccaaccgcc tcccggaaa gcagcgaca acgtcgtcg 
      tctcgttcg cccttcttc gtgcatccc tttttcaaa 
      gggggctcc agcacgaac ctcctggag ttgggaacc 
      accgaaggc cccaacatc acgttgcga cgtcggggc 
      tcctgtgaa tcggggttt tattcaagc gtgggggaa 
      tgtctctct ccgagtagt ttatgggac agtgggaca 
      gcggttagt tcgttgcgc tcgcttgat gagttggaa 
      ccggccgaa ttgcaggcg ttatgtaag agagcgtcg 
      tcaatttgc acagatagc agcgaagat atttcgagt 
      ctgacgggc tccgagtgg acgcaggac ttgaaaccg 
      aagaatatc tcctacatg gtggactac ttcgagcgg 
      aagggggcc agtctgagg cacccgggg ggcgttcga 
      gggggctta cagctcagt tcgaggatt gcagcgctg 
      cgcaaatcg ctggagaag cagggggct gccggtgct 
      gttaattcg aattcgctt gtgacgcca cagaggcac 
      aagtacaac cggattgtt gtctgtgaa ggtgcagtt 
      aggtcaaag ttgcccctt tttgataaa aagtag
    mRNA sequence
    • >TC012191 RA
      aactattta tctgtaatt tcggttcac tttcgtaac 
      aacgataaa tactaaatc gtatttaga atgaaaatt 
      agttatttg cggtgaagc aagaatttg ttccctaaa 
      gttttggaa gtccctcgt atggtggaa gtttcctta 
      ggtgctgcc ctctataag gattgctta gagaattct 
      gctttaccg gtcattttg gtggctgtc agttttaac 
      ctcacttaa ccgtatttt atgtaaggc cgaaattta 
      attcgtaca aagtgattg actgatttt tacctttaa 
      tctagcaaa atgaccaca aaaaatgta ctcgggact 
      ttgacccaa attagattt ttgagctgc cattcagcc 
      agcatcaca agaccccac agattaact tacaagagg 
      acatacccc actgttctt gttaaccca gatgggtca 
      actatcaat attaaatac cacgaaccc aggcaaatc 
      attaaactg cctttgaat atttggact ctcagtgag 
      gctgaaaga aaggcacga ttggaggca agaaaacca 
      aagaagaaa gttaaattt gaggatgat cttgatgat 
      agtttcgac gcaaataaa tatgtaaaa tacttgaaa 
      aagaaatag attaggtaa cattatgta gcgaattgt 
      ctcaataaa gtttaataa ccgtttagt attgttttg 
      aaacagtta attgttgga aatttaaaa tattttgtt 
      aattaaatc gactgaatc ggccacttt cattatggt 
      aatttagct attgtagaa acaattgta tgagtatgg 
      agaagaaag tatgtgaat tacaatttc ctacatggg 
      aacatttca cttttttga aaacgtttt aaaactgtc 
      cattcgcga ttcacaacg ttgtttaat aacaatttc 
      gtaatcgat tcattggct ttgggaacg gaacacgtt 
      tgaaagtgg cgcttcaac ccaaccgcg catgcgtcc 
      gttttgttg ctcaacgtg ccatcgcgc cgtcattgt 
      ttagcccgg cgggaggaa ggagacgca gatatttgc 
      ggatgaagt tttccacaa catggcagc tatgtatac 
      tagatgaac gggtgaaag cttgcactg tcattacaa 
      actttttat ttacgtgtg aaacttcaa attgctagc 
      tcagctctt gatttcagt gcctaaaag tgaggtgtc 
      ttgtgtcga acgaggtgt ctgaatggt gtgcgaagt 
      gtactattt atgtcaagc tgtagcagt gcacacaat 
      ttctaaatt taattataa aacctgcat aaatccatt 
      gcgcgttat atttaccca gaaaagtga tgtcaaatg 
      agctttcgg ccgttttga ccgtcggct gcaatgtcg 
      tgctccgtt tccagtgaa ccacaacat taaaattat 
      gtgtgattt taagacttc aagagtggc ccgcctaag 
      tcgtacaaa aacatgtac gatattggt taacatcac 
      ataggaccc cattctgtt atgatgcac ccgagcagc 
      aaaaatgag ccccgtacc gctaaaatt tcgtgcatt 
      tacgacacc ccctgcgac cccaaagcg accgactac 
      gagaagctg tactgcccc aataacgag ctgtcggag 
      cccccgagg acccgctgt gatagactc aaaactgga 
      tcgtcctgc gaggccctg aagcatgcc gtggcatcc 
      ctccataga ctcgacgac ttccacaag gaaaaaatc 
      ggatcgggg tttttctcg gaggttttc aaggtgacc 
      caccgcgta accggtcaa gtgatggtg ttgaagatg 
      aacttgttg cgttcgaat cgccgtaac atgttgaag 
      gaggtccaa ttgatgaat aaattatcc catccgaat 
      atcctaaat ttgatgggt gtatgcgtg cacgaaggt 
      caattacat gccctcacc gaatacatc aatggtggt 
      tcattagag caactcata caaaataga aacatcgat 
      ttaccacaa ccaacaagg gtatccttg gcaagggat 
      atatcatac ggcatgagc tatcttcat tccaaggac 
      gtatttcac agggactta acaagtaaa aatgtccta 
      ataaagcgc ctggaatcg ggcgagctg caggccgtg 
      gtgggcgac ttcggcttg gcgtcatcg ttccctgac 
      ggcaaaacg aagctgtgt accgtcgga tccccttat 
      tggatgtcc cccgagtgc ctgaaaggt caatactac 
      gaccaacaa agtgatgta tttagttac ggaatagtg 
      ttatgcgaa ctgatagca cgtgtcgaa gctgatccc 
      gatcagttg cccagaact gacaatttc ggtctcgat 
      tatttagcg tttaccgaa atgtgtgga cagaacgtt 
      gtacctgaa tttttaaat ttggcattt cgatgctgc 
      actatcgat ccaaaaagt cggccgaca ttcaacgaa 
      atcgttcaa atattaacc gaaatcttg gccgatcta 
      caacatcat acatccgat cgaggagaa caacaagcc 
      aagttggca agttgtggc gccaaaagc gaggaacaa 
      ctgcctgta cttttggta caatcgtcg gcaccacaa 
      cacaggaaa attgtccac cggcgatcg ttatctgaa 
      gacgtatcc atgttgtcg ctaacgatg cacgcgaca 
      cctagcgag aaagcccgt cgtcacgcc ttgacgatg 
      tgccgtcaa gacccgcac tacaagcct cgtacgagc 
      aatccattc gcggcttta acccaattc cgcggtgtt 
      aaaaagttc ctcggcaat ttttcgtct tgttgcgaa 
      ttgccatct cccttcatc gagtccaca gaatcgccc 
      aaatcgctc cccggctct ccaaccgcc tcccggaaa 
      gcagcgaca acgtcgtcg tctcgttcg cccttcttc 
      gtgcatccc tttttcaaa gggggctcc agcacgaac 
      ctcctggag ttgggaacc accgaaggc cccaacatc 
      acgttgcga cgtcggggc tcctgtgaa tcggggttt 
      tattcaagc gtgggggaa tgtctctct ccgagtagt 
      ttatgggac agtgggaca gcggttagt tcgttgcgc 
      tcgcttgat gagttggaa ccggccgaa ttgcaggcg 
      ttatgtaag agagcgtcg tcaatttgc acagatagc 
      agcgaagat atttcgagt ctgacgggc tccgagtgg 
      acgcaggac ttgaaaccg aagaatatc tcctacatg 
      gtggactac ttcgagcgg aagggggcc agtctgagg 
      cacccgggg ggcgttcga gggggctta cagctcagt 
      tcgaggatt gcagcgctg cgcaaatcg ctggagaag 
      cagggggct gccggtgct gttaattcg aattcgctt 
      gtgacgcca cagaggcac aagtacaac cggattgtt 
      gtctgtgaa ggtgcagtt aggtcaaag ttgcccctt 
      tttgataaa aagtaggcc agtcttcct tcgttttgt 
      ttcttctct agtcagatt gtaattaaa attgttaca 
      tgatttgtg gcctaaaaa gtctatttt tcacgtcct 
      tgttggttc acagttgta catatttgt gtgacctta 
      aaattattg cgtttctag ttttggtaa atatgcggc 
      ctatattag gtcggttta ttggagttg tgcgagaat 
      taagcttcg tttttgcat ttatgttgt tttggacgt 
      tttgaaaga attaagttc cgtttctgt ttcgatttt 
      caaaatcgg ctaaatatt ttatgagtg tggaaaaag 
      actgaaaaa tttacgatt ttcgtgtac taaatattt 
      gctttgttt agctggttt tgatttgtg tgaaccaaa 
      tgccaaatg tataaccga catatccaa gttttggtt 
      gcaataatg taaaaatgt gatgaccgt ccatcagaa 
      aagtattga agattatac caattatgt tactctttg 
      tatttatga aatatttag tgagcaagt attactctt 
      tttaacggt ctgtttgta tatcttcca tattgtctt 
      gtcaataat atatagttg tttagtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY