Status
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Gene information
General information
TC012185 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3685680..3691494
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012185
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01943

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 4

    previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 4
    vitellogenic egg chamber number increased - Number of animals showing the phenotype: 4
    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late potentiallydegenerated - Number of animals showing the phenotype: 4
    vitellogenic egg chamber late shape rounded - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012185 RA
      MKQLIVLAI NLLLLIGYT RADESIGEG TVEKVDLDL 
      GASREGSRT DDEVVKREE EAIKLDGLN VAQLKELRD 
      KAEKFTFQT EVNRMMKLI INSLYRNKE IFLRELISN 
      ASDALDKIR LLSLTDKNV LDSNPELNI RIKADKEAG 
      MLHITDTGI GMTKQDLVN NLGTIAKSG TAEFLSKMQ 
      DASTAQDMN DMIGQFGVG FYSAFLVAD KVVVTTKHN 
      DDKQYIWES DSSSFSIVD DPRGDSLKR GTTVSLQLK 
      PEAKDFLEH DTVRSLVKK YSQFINFPI YMWTSHTEQ 
      VEEPIEEDE KSEEKEKPD TEDEAAVEE EKEEEKPKT 
      KKVDKTVWD WELLNDSKP IWTKKPSEV DDKEYDEFY 
      KSLTKDSKQ PLAKVHFIA EGEVTFKAL LYVPEVQPS 
      ESFNRYGTK TDNIKLYVR RVFITDEFN DMIPSYLSF 
      VRGVVDSDD LPLNVSRET LQQHKLIKV IKKKLVRKV 
      LDMLKKISE EDYEKFWKE YSTNIKLGT IEDPANRTR 
      LAKLLQFHS SNSDKMTSL ADYVKRMKP KQERIFYIA 
      GSSKDEVQK SPFVERLLR KGYEVLYLV EAVDEYAIS 
      AIPEFEGKK FQNVAKEGF SLTESEGGK EQLEQLKTT 
      FEPLTKWLS DEALKDHVA KATVSERLS DSPCALVAS 
      MFGWTGNME RLAISNAHQ KSDDPQRSY YLNQKKTLE 
      INPRHPLMR ELLKRVNDD PSDPTAKDM ALMLFRTAT 
      LRSGYMLRE TADFAQSIE AMMRKTLGV PLDEGVEEE 
      EDLPEDGIP EEESEEIKD DDGDHDEL
    CDS sequence
    • >TC012185 RA
      atgaagcag ttgattgtt ttagcgata aatttgcta 
      cttttaatc ggatacaca cgagcagat gaatctatt 
      ggggaagga accgtggaa aaggttgac ctggacttg 
      ggggctagt agagaaggg tcaagaaca gacgatgaa 
      gtcgttaaa agggaagaa gaagcaatt aaattggac 
      ggacttaat gttgcccaa ttaaaagaa ctgcgcgac 
      aaagcggag aaattcact ttccagacg gaagttaat 
      cggatgatg aaactaatc atcaactcc ttgtaccga 
      aataaagaa attttcctc agggaatta atttctaat 
      gcttctgac gctttagat aagatccgc ttgttgtca 
      ctgacagat aaaaatgtg ttggactca aatcccgag 
      ttgaatatt cgaatcaaa gccgacaag gaggcggga 
      atgcttcat attaccgat actggtatc ggaatgact 
      aaacaagac cttgttaac aacttgggt actattgcg 
      aaatcgggc actgccgaa tttttgagt aaaatgcaa 
      gacgcttca actgctcaa gatatgaac gatatgatt 
      ggtcagttt ggtgttggt ttctattcg gcattctta 
      gttgcggat aaagttgtc gtaacgaca aaacataac 
      gacgataaa cagtacatt tgggagtcg gattcgtca 
      agcttttcc attgtggat gatcctaga ggcgattcg 
      ttgaaaagg ggaaccaca gtcagcctc cagttgaaa 
      cccgaagcc aaagatttc ctcgaacat gacactgtt 
      cgttcttta gttaagaaa tattcacaa tttatcaat 
      ttcccaatc tatatgtgg actagtcat acggaacaa 
      gtggaagaa ccaatcgaa gaagatgaa aagtcggaa 
      gagaaggag aagcctgat actgaagat gaggcagct 
      gttgaggaa gagaaggag gaagaaaaa cccaagact 
      aagaaagtt gataagacg gtttgggac tgggaactg 
      ttgaatgat agtaagcca atttggacg aagaaaccg 
      tcggaagtt gatgataag gaatacgac gagttttac 
      aagtctttg actaaggat agcaaacaa cctttggca 
      aaagttcac tttattgcc gaaggtgaa gttactttc 
      aaggctttg ttgtacgta ccagaagta caaccatct 
      gaaagtttt aacagatat ggtaccaaa accgataat 
      atcaaattg tacgttcga agggtattc attacggat 
      gaatttaac gacatgatt ccatcttat ttgagtttc 
      gttcgaggt gttgtagac tcagacgac ttacctttg 
      aacgtttcc cgtgaaact ctccaacag cacaaacta 
      attaaagtt attaagaag aaactggtt cgcaaagtt 
      ctagatatg ttgaagaag atatctgag gaagattat 
      gagaaattc tggaaggag tactctaca aacatcaaa 
      cttggaaca attgaggat ccagcaaac cgcacccga 
      ttggctaaa ctcctccaa ttccattca tccaacagt 
      gacaaaatg acttcgtta gctgattac gtcaaacgt 
      atgaagccc aaacaagaa cgcattttc tacattgct 
      ggttcatcc aaagacgaa gtccaaaaa tcgcctttc 
      gttgagcga ttgctccgt aaagggtat gaggttttg 
      tacctcgtc gaagccgtc gatgagtat gcgatttcg 
      gcaattccg gaattcgaa ggcaagaag ttccagaat 
      gtggccaag gaaggcttc agcttgact gaaagcgaa 
      ggaggaaag gaacaattg gagcagctt aagaccacc 
      tttgagcct ttgacgaag tggttaagc gatgaagcg 
      ctcaaggac cacgtcgcc aaagctaca gtttcagaa 
      aggttgagt gattccccg tgcgctcta gtcgcgagt 
      atgtttgga tggaccgga aatatggaa cggttagcg 
      atttcaaac gcacaccaa aagtcagac gatcctcaa 
      aggtcgtat tatttgaac cagaagaag acactggag 
      attaaccca aggcatccg ttgatgcgt gagttgttg 
      aagcgggtc aatgacgat ccgtcggat cctacggcc 
      aaagatatg gccttgatg ttgttccgg acggcaacg 
      cttagatcg ggttatatg ttacgggag acggcggat 
      tttgcgcaa tctatcgaa gcgatgatg aggaaaacg 
      ttgggtgtg cccctggat gaaggggtg gaggaagag 
      gaggatctt cccgaagat ggtattccg gaagaagag 
      agtgaggag atcaaggat gatgatggg gatcatgac 
      gagttgtaa
    mRNA sequence
    • >TC012185 RA
      agagctgat tggccacaa tttcggtgt aaactcttc 
      tagaatccg gtcttgtca tcattcgga aattggttt 
      tgatttgtg gtgtcacgt ttgtgccgg tgttgtttc 
      tgaatgaat taaaaaggt ccatttttg ttgaaaaca 
      tgaagcagt tgattgttt tagcgataa atttgctac 
      ttttaatcg gatacacac gagcagatg aatctattg 
      gggaaggaa ccgtggaaa aggttgacc tggacttgg 
      gggctagta gagaagggt caagaacag acgatgaag 
      tcgttaaaa gggaagaag aagcaatta aattggacg 
      gacttaatg ttgcccaat taaaagaac tgcgcgaca 
      aagcggaga aattcactt tccagacgg aagttaatc 
      ggatgatga aactaatca tcaactcct tgtaccgaa 
      ataaagaaa ttttcctca gggaattaa tttctaatg 
      cttctgacg ctttagata agatccgct tgttgtcac 
      tgacagata aaaatgtgt tggactcaa atcccgagt 
      tgaatattc gaatcaaag ccgacaagg aggcgggaa 
      tgcttcata ttaccgata ctggtatcg gaatgacta 
      aacaagacc ttgttaaca acttgggta ctattgcga 
      aatcgggca ctgccgaat ttttgagta aaatgcaag 
      acgcttcaa ctgctcaag atatgaacg atatgattg 
      gtcagtttg gtgttggtt tctattcgg cattcttag 
      ttgcggata aagttgtcg taacgacaa aacataacg 
      acgataaac agtacattt gggagtcgg attcgtcaa 
      gcttttcca ttgtggatg atcctagag gcgattcgt 
      tgaaaaggg gaaccacag tcagcctcc agttgaaac 
      ccgaagcca aagatttcc tcgaacatg acactgttc 
      gttctttag ttaagaaat attcacaat ttatcaatt 
      tcccaatct atatgtgga ctagtcata cggaacaag 
      tggaagaac caatcgaag aagatgaaa agtcggaag 
      agaaggaga agcctgata ctgaagatg aggcagctg 
      ttgaggaag agaaggagg aagaaaaac ccaagacta 
      agaaagttg ataagacgg tttgggact gggaactgt 
      tgaatgata gtaagccaa tttggacga agaaaccgt 
      cggaagttg atgataagg aatacgacg agttttaca 
      agtctttga ctaaggata gcaaacaac ctttggcaa 
      aagttcact ttattgccg aaggtgaag ttactttca 
      aggctttgt tgtacgtac cagaagtac aaccatctg 
      aaagtttta acagatatg gtaccaaaa ccgataata 
      tcaaattgt acgttcgaa gggtattca ttacggatg 
      aatttaacg acatgattc catcttatt tgagtttcg 
      ttcgaggtg ttgtagact cagacgact tacctttga 
      acgtttccc gtgaaactc tccaacagc acaaactaa 
      ttaaagtta ttaagaaga aactggttc gcaaagttc 
      tagatatgt tgaagaaga tatctgagg aagattatg 
      agaaattct ggaaggagt actctacaa acatcaaac 
      ttggaacaa ttgaggatc cagcaaacc gcacccgat 
      tggctaaac tcctccaat tccattcat ccaacagtg 
      acaaaatga cttcgttag ctgattacg tcaaacgta 
      tgaagccca aacaagaac gcattttct acattgctg 
      gttcatcca aagacgaag tccaaaaat cgcctttcg 
      ttgagcgat tgctccgta aagggtatg aggttttgt 
      acctcgtcg aagccgtcg atgagtatg cgatttcgg 
      caattccgg aattcgaag gcaagaagt tccagaatg 
      tggccaagg aaggcttca gcttgactg aaagcgaag 
      gaggaaagg aacaattgg agcagctta agaccacct 
      ttgagcctt tgacgaagt ggttaagcg atgaagcgc 
      tcaaggacc acgtcgcca aagctacag tttcagaaa 
      ggttgagtg attccccgt gcgctctag tcgcgagta 
      tgtttggat ggaccggaa atatggaac ggttagcga 
      tttcaaacg cacaccaaa agtcagacg atcctcaaa 
      ggtcgtatt atttgaacc agaagaaga cactggaga 
      ttaacccaa ggcatccgt tgatgcgtg agttgttga 
      agcgggtca atgacgatc cgtcggatc ctacggcca 
      aagatatgg ccttgatgt tgttccgga cggcaacgc 
      ttagatcgg gttatatgt tacgggaga cggcggatt 
      ttgcgcaat ctatcgaag cgatgatga ggaaaacgt 
      tgggtgtgc ccctggatg aaggggtgg aggaagagg 
      aggatcttc ccgaagatg gtattccgg aagaagaga 
      gtgaggaga tcaaggatg atgatgggg atcatgacg 
      agttgtaaa tagtgtgag tgtgtgtgt gataatgat 
      tgtttaacg atcatcaaa gactgtcta atttttgta 
      tcgtacctc tttaaatta taataaaca atacttaaa 
      tcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY