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Gene information
General information
TC012169 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3961541..3969190
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012169
    iBeetle screen iB_01938

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012169 RA
      MAAKEAVYV QDACKSYGK KEVLKNLRM KVPRGCIYG 
      LLGPSGCGK TTLLSCIVG RRNLNSGDI WVLGGKPGT 
      VDSGVPGPK VGYMPQDIA LVGEFTVKD AIFYFGRIF 
      DMPEKMLEE RFNELSSLL DLPPDDRFI RNCSGGQQR 
      RVSFAASMV HLPELLILD EPTVGVDPI LRERIWNYL 
      VEITSKSNM AVIITTHYI EEARQANLI GLMRGGRLL 
      AEESPATLL TMFQTEVLE DVFFILSQK QSEGRLDDT 
      MAVARSNLQ IQNSNTDIS VASFDTTRG STDILTKEK 
      QTKTKNKSG YELNGKRMK SLLDKNWKQ FYRNVSGMI 
      FILMFPIAQ NVAFLLAVG PDPKGINLA VVNEESFAT 
      LCPNFNFSQ SAVPYDDYN CHFHNMSCR FLSYIDTPM 
      ITKVRYDAL PDALEAVKH GEVVGVMYM AENFTESFE 
      ARVEEGRDI EAEVLSFSE IKVWLDMSN RQIGATVKY 
      KLLSIFKDF QKDLLRDCN YEPQMADFM NMTTFYGKD 
      GDTFTEYMT PGLVLTIMF FLMTLMTSQ IIVTDRSEG 
      LWDRSIIAG VSSLEISLT HFIFQIGIV LIYTVTTLT 
      ITFAIFKIP NSGSMWIIT LITFFQGLT GVGFGFWIS 
      IISENVTMA NVLTTGSFY IMILLSGLM WPLEGMPIV 
      LQWVSKCLP FTIAIDSFR NVMKKGWSF DYFEVWNGF 
      GIEITWIVI FAVVNTMLI QSKR
    CDS sequence
    • >TC012169 RA
      atggcggcc aaagaagcc gtttatgtt caagacgcc 
      tgcaaatcg tacggaaaa aaggaagtt ttgaaaaac 
      ctccgaatg aaagtgcct cgaggatgc atttacggt 
      ctccttggt ccaagcggt tgtggcaag acaaccctc 
      ctaagctgc atcgtaggc cgccgcaac ttgaactcc 
      ggcgacatc tgggtcctg gggggcaag cccggcacc 
      gtggacagc ggggttccg ggcccaaaa gtgggctac 
      atgccccag gatatcgcc ctcgtgggc gaattcacc 
      gtcaaggat gcgattttt tactttggc cgcatcttc 
      gacatgcct gaaaaaatg ctcgaagag agatttaac 
      gaactttcc agcttgctg gatttgccg cctgatgat 
      cgattcata aggaactgc agtggaggt cagcaacgg 
      cgcgtctcc ttcgctgcc tccatggtg cacttgccg 
      gagttgctc attctggat gagcccacg gtgggcgtg 
      gaccccatc ttgcgcgaa aggatatgg aattatctc 
      gtggaaatc acgagcaag tcaaacatg gctgtgatt 
      attaccacg cattatatc gaagaagcg cggcaagcg 
      aatttgatc ggtttaatg cgcggtggg cgcctcctg 
      gccgaggaa tccccggcc actctcctc accatgttt 
      caaaccgag gtcctcgag gatgttttc ttcatcctg 
      agccagaag cagtccgag ggacgcctc gacgacacc 
      atggctgtg gcccgcagt aacttacaa atccagaac 
      tcaaacact gacatcagc gttgcgtcg tttgacacg 
      actcgggga tccaccgat atcctcacg aaagagaaa 
      caaaccaaa accaaaaac aagagcggc tacgaactg 
      aacggcaaa cggatgaag agcctcctc gacaaaaac 
      tggaagcaa ttctaccga aatgtcagt ggaatgatt 
      ttcattctg atgtttccc atcgcacag aacgtggct 
      tttctcctc gccgtcggg cccgacccc aagggcatc 
      aatctggct gtcgtaaac gaggaaagc tttgccacg 
      ttgtgtccg aatttcaac ttctcgcaa tcggcggtg 
      ccttacgat gactacaat tgccatttt cataacatg 
      agttgccgg tttttgagc tacatagac accccgatg 
      ataaccaaa gtgagatat gacgcgtta ccggacgct 
      ttggaagcg gtaaagcat ggagaagtc gtcggggtg 
      atgtacatg gcggagaat ttcaccgaa tcgttcgaa 
      gccagagtc gaagaaggg agagatatc gaagctgag 
      gttctaagt ttcagtgaa attaaagtg tggctcgac 
      atgtcaaac cggcaaatt ggggcaact gttaagtac 
      aaacttttg tcgatattt aaggatttt caaaaggat 
      ttgctcaga gattgtaac tacgagcct caaatggcc 
      gacttcatg aacatgact acgttttac ggcaaggat 
      ggagacaca tttactgag tatatgaca ccggggcta 
      gtcctaaca attatgttt ttcctaatg actctaatg 
      acgtcgcaa atcatcgta acagaccgc agtgagggc 
      ctttgggac cgctccata atcgcagga gtgtcgtct 
      ctcgaaatc agtttaaca cactttatt ttccaaatc 
      ggaatcgtc ctcatttac acagttacc accctcacg 
      ataacgttc gcaatattt aaaatacca aacagtggc 
      agtatgtgg atcatcaca ctgatcacc ttcttccaa 
      ggattaaca ggagtgggt tttggtttt tggatttca 
      attatttcg gaaaacgtg accatggct aacgtgctc 
      acaactgga agtttctac atcatgatt ctactaagt 
      ggcctcatg tggcccctt gaaggcatg cctattgtt 
      ctccagtgg gtgtcgaaa tgtctcccg ttcacaatc 
      gcaatagat tcgttccga aatgtaatg aaaaaaggg 
      tggtctttt gactacttc gaggtgtgg aacggtttc 
      gggatcgaa ataacatgg attgttatt tttgcagtg 
      gtgaacaca atgttaatc caaagtaaa cgataa
    mRNA sequence
    • >TC012169 RA
      tgcggttcc agaaaagtg cgtgttttc ttggtgaaa 
      actagttcc gtctgcaag agcagttgc tccacacca 
      gcagaaaca cctaacggt tgtgcacgt ttgatttaa 
      agtgcaaat atttcatta cgcaaatgt aatttgctg 
      aattggcaa ctgaagtaa aattaaaca agcagctta 
      cagcttgta aaagtggtc tgtgcgttt gattagtga 
      ctttgtgac aatctccta tttttcttc accgattcc 
      ttcagaggt agagtcaag gttaataat agcgatttc 
      ctgctgaaa agttgtgaa tgtgatact tagtcgtgt 
      cggaccagt tcgcatgtg agcagtggt tcgagaaga 
      ctgcagcca gtgtcttgg atttgtgga tacgaagga 
      gccgtcgac atggcggcc aaagaagcc gtttatgtt 
      caagacgcc tgcaaatcg tacggaaaa aaggaagtt 
      ttgaaaaac ctccgaatg aaagtgcct cgaggatgc 
      atttacggt ctccttggt ccaagcggt tgtggcaag 
      acaaccctc ctaagctgc atcgtaggc cgccgcaac 
      ttgaactcc ggcgacatc tgggtcctg gggggcaag 
      cccggcacc gtggacagc ggggttccg ggcccaaaa 
      gtgggctac atgccccag gatatcgcc ctcgtgggc 
      gaattcacc gtcaaggat gcgattttt tactttggc 
      cgcatcttc gacatgcct gaaaaaatg ctcgaagag 
      agatttaac gaactttcc agcttgctg gatttgccg 
      cctgatgat cgattcata aggaactgc agtggaggt 
      cagcaacgg cgcgtctcc ttcgctgcc tccatggtg 
      cacttgccg gagttgctc attctggat gagcccacg 
      gtgggcgtg gaccccatc ttgcgcgaa aggatatgg 
      aattatctc gtggaaatc acgagcaag tcaaacatg 
      gctgtgatt attaccacg cattatatc gaagaagcg 
      cggcaagcg aatttgatc ggtttaatg cgcggtggg 
      cgcctcctg gccgaggaa tccccggcc actctcctc 
      accatgttt caaaccgag gtcctcgag gatgttttc 
      ttcatcctg agccagaag cagtccgag ggacgcctc 
      gacgacacc atggctgtg gcccgcagt aacttacaa 
      atccagaac tcaaacact gacatcagc gttgcgtcg 
      tttgacacg actcgggga tccaccgat atcctcacg 
      aaagagaaa caaaccaaa accaaaaac aagagcggc 
      tacgaactg aacggcaaa cggatgaag agcctcctc 
      gacaaaaac tggaagcaa ttctaccga aatgtcagt 
      ggaatgatt ttcattctg atgtttccc atcgcacag 
      aacgtggct tttctcctc gccgtcggg cccgacccc 
      aagggcatc aatctggct gtcgtaaac gaggaaagc 
      tttgccacg ttgtgtccg aatttcaac ttctcgcaa 
      tcggcggtg ccttacgat gactacaat tgccatttt 
      cataacatg agttgccgg tttttgagc tacatagac 
      accccgatg ataaccaaa gtgagatat gacgcgtta 
      ccggacgct ttggaagcg gtaaagcat ggagaagtc 
      gtcggggtg atgtacatg gcggagaat ttcaccgaa 
      tcgttcgaa gccagagtc gaagaaggg agagatatc 
      gaagctgag gttctaagt ttcagtgaa attaaagtg 
      tggctcgac atgtcaaac cggcaaatt ggggcaact 
      gttaagtac aaacttttg tcgatattt aaggatttt 
      caaaaggat ttgctcaga gattgtaac tacgagcct 
      caaatggcc gacttcatg aacatgact acgttttac 
      ggcaaggat ggagacaca tttactgag tatatgaca 
      ccggggcta gtcctaaca attatgttt ttcctaatg 
      actctaatg acgtcgcaa atcatcgta acagaccgc 
      agtgagggc ctttgggac cgctccata atcgcagga 
      gtgtcgtct ctcgaaatc agtttaaca cactttatt 
      ttccaaatc ggaatcgtc ctcatttac acagttacc 
      accctcacg ataacgttc gcaatattt aaaatacca 
      aacagtggc agtatgtgg atcatcaca ctgatcacc 
      ttcttccaa ggattaaca ggagtgggt tttggtttt 
      tggatttca attatttcg gaaaacgtg accatggct 
      aacgtgctc acaactgga agtttctac atcatgatt 
      ctactaagt ggcctcatg tggcccctt gaaggcatg 
      cctattgtt ctccagtgg gtgtcgaaa tgtctcccg 
      ttcacaatc gcaatagat tcgttccga aatgtaatg 
      aaaaaaggg tggtctttt gactacttc gaggtgtgg 
      aacggtttc gggatcgaa ataacatgg attgttatt 
      tttgcagtg gtgaacaca atgttaatc caaagtaaa 
      cgataattt taagttgtt gtaaatgag ataaattaa 
      ataaagctg ttttattgt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY