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Gene information
General information
TC012168 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3974731..3981000
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012168
    iBeetle screen iB_01937

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late potentiallydegenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012168 RA
      MENLFSMWK VRELADKVT NVVMNYTEI EAKVREATN 
      DEPWGPTGQ IMQELAHST FTYEHFPEV MSMLWKRMF 
      QDNKQHWRR IYKALLVLN YLVKNGSER VVTSAREHI 
      YDLRSLENF SFIDDMGKD QGVNIRHKV KELIDFIQD 
      DDRLREERK KAKKNKDKY IGMSSDMVG MRLGSHERW 
      DDKPRDYGD HDWEEPSSG ASRYRDRSF DDEYDIDKE 
      DSDTESARN HTSRKYKDN DPSPTEKRV NININSAIT 
      NTPKKSTKP IKKVDLGAA ANFGRDASQ SPLPQASDL 
      IDDFNPRAE EEEKKSTEF GDFEAAFGS NATTTPKSD 
      DDFADFSSA FSQNNTDAN LMNQNQNLF MPNVIPPPN 
      LGIQPPIGG LMGAQAPVM AAKPQNSND LLGDLSGFS 
      GLSIQPPGG FGNVPNNNQ GLVGAAPSL LDGGPLLTP 
      TSAQQPLQP LSSASSNNN TEVKTPANK NQQNIGSTW 
      SNSGNLNID LDNLLVTKP KQGVSPSMN QLASNPTSP 
      VNQPKLTNQ YAMNFNAQG FPQTNAFNQ PNKQLFPSF
    • >TC012168 RB
      MENLFSMWK VRELADKVT NVVMNYTEI EAKVREATN 
      DEPWGPTGQ IMQELAHST FTYEHFPEV MSMLWKRMF 
      QDNKQHWRR IYKALLVLN YLVKNGSER VVTSAREHI 
      YDLRSLENF SFIDDMGKD QGVNIRHKV KELIDFIQD 
      DDRLREERK KAKKNKDKY IGMSSDMVG MRLGSHERW 
      DDKPRDYGD HDWEEPSSG ASRYRDRSF DDEYDIDKE 
      DSDTESARN HTSRKYKDN DPSPTEKRV NININSAIT 
      NTPKKSTKP IKKVDLGAA ANFGRDASQ SPLPQASDL 
      IDDFNPRAE EEEKKSTEF GDFEAAFGS NATTTPKSD 
      DDFADFSSA FSQNNTDAN LMNQNQNLF MPNVIPPPN 
      LGIQPPIGG LMGAQAPVM AAKPQNSND LLGDLSGFS 
      GLSIQPPGG FGNVPNNNQ GLVGAAPSL LDGDPVREV 
      LCDRDSGKN SVEKQLRDA SATVVNKLN KIKKISSQS 
      DIDDIINSL DKFLPLLPT LTVQKLLEI DSTDLSHYA 
      DREFRNLLE NLIAKFDQN FPLIGGQVY KEVKVVFSV 
      EDYHFFRVT CDVIVKNLK TNLKILNSL SVLAEIALK 
      SEGCFALIW HNFFTKEED LWQNDVKIL ITFPNRISN 
      ALQGQFNQF FKPNEYSKF LVFNVIKVI EFSTDLLLR 
      EPKYEKNFY YTNLALFLS KTLINYNEH YNSGAIKTF 
      VKVANILTN QKSDKLNVY RTVFWNLFE SLERSAVEI 
      LAVTVLQNT DPSVYTIKS VLGPNLIKC DNWKYVLCT 
      KIPLLSHYD NENLIKNLI VYLSATSEH NLFTLLVNL 
      ITVWSDKSA LNHTSVEHH LYLTKLIVV IINSFKNIG 
      LSEHQRQIL QSKMYSGVP VHLENSTPS VRAMGMKVS 
      EIVLNFIQT ESEKSETLR FEFDQMPNE GKEVGEILQ 
      KLFDTDMTI YFKNKDVEE DVFPLISSL LEAEKCPEY 
      VPPARQLRG QEEPKNVVV AEAIHQKNN LLTIVDSTD 
      FELDSDDDL EPYDLANDV KVSKKEPPK YLRDLRDGL 
      LETQDCDVF SLSLEACED LIIKQLPDD DVSLGVELL 
      QILVGLDQR FYVENFDSV VFKSCVAIT CVYPSQSAE 
      YLCKWFHAD VGTYSISHR IFLLDVLSE ASRNLSALT 
      LDKPDEKIV IKRRKKNPD LEKAEEIIK KRLESKTRY 
      FHKHKAFRV EKLNKFAQV AGHFFFPLL YGYNGNKMI 
      SQIPQNDSD FILLIHFIR TLSVIMCSS QNCPIEPRM 
      AKEVLHFSW YLRFHKEVK VRIAVIGLI GAAVINTPK 
      EILMSDFTS ELLEFRLWL GDLLSLSVT RGEPNSECR 
      SLAASTMCI IDSFLKVDD E
    CDS sequence
    • >TC012168 RA
      atggaaaat ttatttagt atgtggaaa gtgcgggaa 
      ctcgccgac aaggttaca aatgttgtt atgaattac 
      accgaaatc gaagccaaa gtcagggag gccacgaac 
      gacgagcca tgggggccc actggccag atcatgcaa 
      gagctggct cactccact tttacttac gaacatttc 
      cccgaagta atgtcaatg ttgtggaaa cgaatgttt 
      caggacaat aagcaacac tggaggcgg atttacaag 
      gcgctcttg gtgctgaat tatttagta aagaatggg 
      tctgaacgc gtcgttact tcggccagg gagcacatt 
      tacgacctc aggtctcta gaaaatttt agttttatt 
      gatgatatg gggaaggac caaggggtt aacattagg 
      cacaaagtg aaggagctc atagacttt atccaggac 
      gacgaccgt ctgcgcgag gagcggaaa aaggccaaa 
      aagaacaaa gacaagtac atcgggatg agctcagac 
      atggtgggc atgaggcta ggaagtcac gagaggtgg 
      gacgacaag ccccgggac tacggcgac cacgactgg 
      gaggagccc tcctcgggg gcgagtcgc taccgcgac 
      cgctccttt gacgacgag tacgacatt gataaggaa 
      gacagcgac accgaatcg gcccgcaac cacaccagc 
      cgcaaatac aaagacaac gacccaagc cccaccgaa 
      aagcgcgtc aacatcaac ataaactcg gccataaca 
      aacaccccg aaaaagtcc accaagccg atcaaaaaa 
      gtcgaccta ggggcagct gcgaatttc ggccgcgat 
      gctagtcag agccccttg ccacaggcc agcgacctg 
      atcgacgat ttcaacccg cgtgccgag gaagaggag 
      aaaaagtcg acggagttt ggcgatttt gaggcagct 
      tttgggtca aatgcgacg acgacgccg aaatccgac 
      gacgatttt gccgatttc agttcagcg ttttcgcag 
      aacaacacc gacgcgaat ttgatgaac cagaatcag 
      aatttgttc atgccgaat gtcattccg ccccccaat 
      ttggggata cagccgccg atagggggg ctcatgggg 
      gcgcaggcg ccggtgatg gcagcgaag ccgcagaat 
      agcaacgat ttgttgggg gatttgtcg ggattcagt 
      ggtttgagc atacagccc ccagggggg ttcgggaat 
      gttccaaat aataatcag gggctcgtg ggggcggcg 
      cccagtttg ttggatgga ggtccgttg ctaaccccc 
      acatcggcc caacagccc cttcaaccg ttgtcttcc 
      gcatcgtcc aataacaac accgaggtt aaaacacca 
      gcaaataaa aaccaacag aatatcggg tcgacgtgg 
      agcaactca ggcaactta aatatcgat ttagataat 
      ttactcgtg acgaaacca aaacaagga gtctcacct 
      agtatgaac caattggcg agcaatcca acctcaccc 
      gttaatcaa ccgaaatta acaaatcaa tatgcgatg 
      aacttcaac gctcaaggc ttcccccaa acgaatgct 
      ttcaatcag ccaaataag caactgttt ccaagcttt 
      taa
    • >TC012168 RB
      atggaaaat ttatttagt atgtggaaa gtgcgggaa 
      ctcgccgac aaggttaca aatgttgtt atgaattac 
      accgaaatc gaagccaaa gtcagggag gccacgaac 
      gacgagcca tgggggccc actggccag atcatgcaa 
      gagctggct cactccact tttacttac gaacatttc 
      cccgaagta atgtcaatg ttgtggaaa cgaatgttt 
      caggacaat aagcaacac tggaggcgg atttacaag 
      gcgctcttg gtgctgaat tatttagta aagaatggg 
      tctgaacgc gtcgttact tcggccagg gagcacatt 
      tacgacctc aggtctcta gaaaatttt agttttatt 
      gatgatatg gggaaggac caaggggtt aacattagg 
      cacaaagtg aaggagctc atagacttt atccaggac 
      gacgaccgt ctgcgcgag gagcggaaa aaggccaaa 
      aagaacaaa gacaagtac atcgggatg agctcagac 
      atggtgggc atgaggcta ggaagtcac gagaggtgg 
      gacgacaag ccccgggac tacggcgac cacgactgg 
      gaggagccc tcctcgggg gcgagtcgc taccgcgac 
      cgctccttt gacgacgag tacgacatt gataaggaa 
      gacagcgac accgaatcg gcccgcaac cacaccagc 
      cgcaaatac aaagacaac gacccaagc cccaccgaa 
      aagcgcgtc aacatcaac ataaactcg gccataaca 
      aacaccccg aaaaagtcc accaagccg atcaaaaaa 
      gtcgaccta ggggcagct gcgaatttc ggccgcgat 
      gctagtcag agccccttg ccacaggcc agcgacctg 
      atcgacgat ttcaacccg cgtgccgag gaagaggag 
      aaaaagtcg acggagttt ggcgatttt gaggcagct 
      tttgggtca aatgcgacg acgacgccg aaatccgac 
      gacgatttt gccgatttc agttcagcg ttttcgcag 
      aacaacacc gacgcgaat ttgatgaac cagaatcag 
      aatttgttc atgccgaat gtcattccg ccccccaat 
      ttggggata cagccgccg atagggggg ctcatgggg 
      gcgcaggcg ccggtgatg gcagcgaag ccgcagaat 
      agcaacgat ttgttgggg gatttgtcg ggattcagt 
      ggtttgagc atacagccc ccagggggg ttcgggaat 
      gttccaaat aataatcag gggctcgtg ggggcggcg 
      cccagtttg ttggatgga gatccagtt cgggaagtg 
      ttgtgtgat cgtgattca ggaaaaaat tcagtggaa 
      aaacagtta cgagatgct agtgccacc gttgtgaac 
      aaattgaat aaaatcaag aaaatttca tcgcaaagt 
      gatatcgac gatataatc aacagtctc gataaattt 
      ttaccacta ttgccaact ctaacagta caaaaatta 
      ctcgaaata gactcaaca gatttaagt cactatgcc 
      gatcgagaa tttcgaaat cttctagag aatttaata 
      gcgaaattt gaccaaaat tttccactg atcggcgga 
      caggtttat aaagaagtg aaagttgtg ttttccgtg 
      gaagattac cactttttc cgagtgact tgcgacgtc 
      attgtgaaa aatttaaaa accaacttg aaaatttta 
      aattcactc agtgttttg gctgaaata gctctgaaa 
      agtgagggt tgtttcgcc ctaatttgg cacaatttc 
      ttcacaaag gaggaggat ttgtggcaa aacgacgtg 
      aaaatatta attacgttt ccaaatcga atctcaaac 
      gctcttcaa ggccaattc aaccaattt ttcaaacca 
      aacgagtat tcaaaattc ctggttttc aacgtaatc 
      aaagtgatt gaattttca accgacttg ctactccgg 
      gaaccaaaa tatgaaaaa aatttctat tacacaaac 
      ctggcattg tttttaagc aaaactttg attaactac 
      aacgaacat tacaattcc ggtgctatt aaaactttc 
      gtaaaagtt gcaaatatt ctgactaac caaaagtca 
      gacaaactt aatgtttat cgaacagta ttttggaat 
      ttgttcgaa agtttggaa cggagcgct gtagaaatc 
      ctagcagtg acagttttg caaaatact gacccttcc 
      gtttacaca attaaaagt gttctaggc cccaattta 
      atcaaatgt gataactgg aagtacgtt ttgtgtaca 
      aaaatcccc ctgttatca cattacgat aacgagaat 
      ctcattaaa aatttaata gtgtattta agtgccacg 
      tccgagcac aatttgttc acactttta gtgaaccta 
      atcactgtt tggtccgac aaatcggcc ctcaaccac 
      acgagtgtg gaacaccac ttgtattta acaaaacta 
      atcgttgtt ataataaac agtttcaaa aatattggc 
      ctaagtgag caccagagg cagattttg cagtcaaaa 
      atgtactcg ggggtcccc gtccacttg gagaactcg 
      accccttct gtgagggcc atgggcatg aaagtgtcg 
      gagattgtt ttgaatttt atccaaact gagagcgaa 
      aaaagcgaa actttgaga ttcgagttc gatcaaatg 
      cccaacgag gggaaagaa gttggcgaa attttgcaa 
      aaactgttc gatacggac atgacgatt tacttcaag 
      aataaagac gttgaagag gatgttttc cccttgatt 
      tcaagtctt ttagaggcg gaaaagtgc cccgagtat 
      gtgccccca gcgaggcaa ttgcggggg caagaggag 
      cccaaaaat gtggtcgtg gctgaagcc atccaccaa 
      aaaaataat ctgctcacg attgtagac agtaccgac 
      ttcgagttg gactcagat gatgatctg gagccttac 
      gatttggca aacgatgtg aaagtgtcg aaaaaggag 
      cccccgaag tacttgcgg gacttgaga gacggttta 
      ttggagacg caagattgc gacgttttt agtttaagt 
      ttggaagcg tgcgaggat ttgattatt aagcagcta 
      cccgacgat gatgtctca cttggggtc gagttactg 
      caaattttg gtcggacta gaccaacgt ttttacgtt 
      gaaaacttc gattctgtg gtgtttaaa agctgcgtt 
      gcgataact tgtgtttat ccatcacaa agtgctgaa 
      tatttatgc aagtggttt catgccgac gttgggact 
      tattcgatc tctcaccgc attttccta cttgatgtt 
      ttaagtgaa gcttcgagg aatttgtcg gctttaacg 
      ctggacaag ccggatgaa aaaatcgta atcaagcgc 
      aggaagaaa aaccctgac ttggagaag gccgaagaa 
      ataattaaa aaacgcctc gagtccaaa actcggtac 
      ttccacaaa cacaaggct ttccgagtg gaaaaactg 
      aataagttt gcacaagtg gccggccac ttctttttt 
      ccgttactg tacggttat aatggcaat aaaatgatt 
      tcgcaaatt ccccaaaac gacagtgat tttattctg 
      ttgattcac tttattcgg accctgtct gttatcatg 
      tgttcctcg caaaattgt ccgattgaa ccgcgaatg 
      gccaaagaa gtgttacat ttctcgtgg tatttgcgc 
      ttccacaag gaggttaaa gtgaggatt gctgtcatc 
      ggtttgata ggagctgca gttataaac acgccgaaa 
      gagattttg atgtcggac ttcaccagc gaattgctc 
      gagttcagg ctttggttg ggggattta ctgagcttg 
      agcgtgacc cgaggagag ccgaattcg gagtgtcgg 
      agtttagcg gctagtact atgtgcatt attgatagt 
      ttcttgaaa gttgacgat gaataa
    mRNA sequence
    • >TC012168 RA
      tactgttga aaaaatcgt ccaattttt gcttattat 
      tgatagaaa ttaataatt tgtgttggt gagtttggc 
      ttggcaagt ggcgggcct gtcgtgatg tcattgcgc 
      taggaaata tggctgact gatcgccaa gtcaccttt 
      tgccaaata atttccact tgtttgtgt gataaaccc 
      gggaaaagc cagactttt aacgtatta ttagagatc 
      tcaagccag gcaaaatgg aaaatttat ttagtatgt 
      ggaaagtgc gggaactcg ccgacaagg ttacaaatg 
      ttgttatga attacaccg aaatcgaag ccaaagtca 
      gggaggcca cgaacgacg agccatggg ggcccactg 
      gccagatca tgcaagagc tggctcact ccactttta 
      cttacgaac atttccccg aagtaatgt caatgttgt 
      ggaaacgaa tgtttcagg acaataagc aacactgga 
      ggcggattt acaaggcgc tcttggtgc tgaattatt 
      tagtaaaga atgggtctg aacgcgtcg ttacttcgg 
      ccagggagc acatttacg acctcaggt ctctagaaa 
      attttagtt ttattgatg atatgggga aggaccaag 
      gggttaaca ttaggcaca aagtgaagg agctcatag 
      actttatcc aggacgacg accgtctgc gcgaggagc 
      ggaaaaagg ccaaaaaga acaaagaca agtacatcg 
      ggatgagct cagacatgg tgggcatga ggctaggaa 
      gtcacgaga ggtgggacg acaagcccc gggactacg 
      gcgaccacg actgggagg agccctcct cgggggcga 
      gtcgctacc gcgaccgct cctttgacg acgagtacg 
      acattgata aggaagaca gcgacaccg aatcggccc 
      gcaaccaca ccagccgca aatacaaag acaacgacc 
      caagcccca ccgaaaagc gcgtcaaca tcaacataa 
      actcggcca taacaaaca ccccgaaaa agtccacca 
      agccgatca aaaaagtcg acctagggg cagctgcga 
      atttcggcc gcgatgcta gtcagagcc ccttgccac 
      aggccagcg acctgatcg acgatttca acccgcgtg 
      ccgaggaag aggagaaaa agtcgacgg agtttggcg 
      attttgagg cagcttttg ggtcaaatg cgacgacga 
      cgccgaaat ccgacgacg attttgccg atttcagtt 
      cagcgtttt cgcagaaca acaccgacg cgaatttga 
      tgaaccaga atcagaatt tgttcatgc cgaatgtca 
      ttccgcccc ccaatttgg ggatacagc cgccgatag 
      gggggctca tgggggcgc aggcgccgg tgatggcag 
      cgaagccgc agaatagca acgatttgt tgggggatt 
      tgtcgggat tcagtggtt tgagcatac agcccccag 
      gggggttcg ggaatgttc caaataata atcaggggc 
      tcgtggggg cggcgccca gtttgttgg atggaggtc 
      cgttgctaa cccccacat cggcccaac agccccttc 
      aaccgttgt cttccgcat cgtccaata acaacaccg 
      aggttaaaa caccagcaa ataaaaacc aacagaata 
      tcgggtcga cgtggagca actcaggca acttaaata 
      tcgatttag ataatttac tcgtgacga aaccaaaac 
      aaggagtct cacctagta tgaaccaat tggcgagca 
      atccaacct cacccgtta atcaaccga aattaacaa 
      atcaatatg cgatgaact tcaacgctc aaggcttcc 
      cccaaacga atgctttca atcagccaa ataagcaac 
      tgtttccaa gcttttaaa gcacctaat ttttattcg 
      aaattccaa agattgtac tttaatcgt tgagattga 
      gccaattgg tcttgcgtt tatagtttt taaaaacgg 
      ttttcttgc acatatttt gtatagtcc gtttggttg 
      tattacaat gctcatgag ggtgctggg tacatttaa 
      gtttgttca tattttatt tagtattac gcaactatt 
      tatacaact aatttgtag gaacagcaa aatcggctc 
      tgttagcaa acagataaa gcaatattg cattgttaa 
      taattttct ataaaataa aaacaaatt cgacctatc 
      ggcagtttt ttctttttc gttgaaggt tgcgatacc 
      gtggtgcct tattatcga taacactat ccatgttac 
      agttagtaa ctgtttgaa cattccgct catttagtt 
      tattgtaaa aacatataa aataaattg ttgtgaata 
      tt
    • >TC012168 RB
      tactgttga aaaaatcgt ccaattttt gcttattat 
      tgatagaaa ttaataatt tgtgttggt gagtttggc 
      ttggcaagt ggcgggcct gtcgtgatg tcattgcgc 
      taggaaata tggctgact gatcgccaa gtcaccttt 
      tgccaaata atttccact tgtttgtgt gataaaccc 
      gggaaaagc cagactttt aacgtatta ttagagatc 
      tcaagccag gcaaaatgg aaaatttat ttagtatgt 
      ggaaagtgc gggaactcg ccgacaagg ttacaaatg 
      ttgttatga attacaccg aaatcgaag ccaaagtca 
      gggaggcca cgaacgacg agccatggg ggcccactg 
      gccagatca tgcaagagc tggctcact ccactttta 
      cttacgaac atttccccg aagtaatgt caatgttgt 
      ggaaacgaa tgtttcagg acaataagc aacactgga 
      ggcggattt acaaggcgc tcttggtgc tgaattatt 
      tagtaaaga atgggtctg aacgcgtcg ttacttcgg 
      ccagggagc acatttacg acctcaggt ctctagaaa 
      attttagtt ttattgatg atatgggga aggaccaag 
      gggttaaca ttaggcaca aagtgaagg agctcatag 
      actttatcc aggacgacg accgtctgc gcgaggagc 
      ggaaaaagg ccaaaaaga acaaagaca agtacatcg 
      ggatgagct cagacatgg tgggcatga ggctaggaa 
      gtcacgaga ggtgggacg acaagcccc gggactacg 
      gcgaccacg actgggagg agccctcct cgggggcga 
      gtcgctacc gcgaccgct cctttgacg acgagtacg 
      acattgata aggaagaca gcgacaccg aatcggccc 
      gcaaccaca ccagccgca aatacaaag acaacgacc 
      caagcccca ccgaaaagc gcgtcaaca tcaacataa 
      actcggcca taacaaaca ccccgaaaa agtccacca 
      agccgatca aaaaagtcg acctagggg cagctgcga 
      atttcggcc gcgatgcta gtcagagcc ccttgccac 
      aggccagcg acctgatcg acgatttca acccgcgtg 
      ccgaggaag aggagaaaa agtcgacgg agtttggcg 
      attttgagg cagcttttg ggtcaaatg cgacgacga 
      cgccgaaat ccgacgacg attttgccg atttcagtt 
      cagcgtttt cgcagaaca acaccgacg cgaatttga 
      tgaaccaga atcagaatt tgttcatgc cgaatgtca 
      ttccgcccc ccaatttgg ggatacagc cgccgatag 
      gggggctca tgggggcgc aggcgccgg tgatggcag 
      cgaagccgc agaatagca acgatttgt tgggggatt 
      tgtcgggat tcagtggtt tgagcatac agcccccag 
      gggggttcg ggaatgttc caaataata atcaggggc 
      tcgtggggg cggcgccca gtttgttgg atggagatc 
      cagttcggg aagtgttgt gtgatcgtg attcaggaa 
      aaaattcag tggaaaaac agttacgag atgctagtg 
      ccaccgttg tgaacaaat tgaataaaa tcaagaaaa 
      tttcatcgc aaagtgata tcgacgata taatcaaca 
      gtctcgata aatttttac cactattgc caactctaa 
      cagtacaaa aattactcg aaatagact caacagatt 
      taagtcact atgccgatc gagaatttc gaaatcttc 
      tagagaatt taatagcga aatttgacc aaaattttc 
      cactgatcg gcggacagg tttataaag aagtgaaag 
      ttgtgtttt ccgtggaag attaccact ttttccgag 
      tgacttgcg acgtcattg tgaaaaatt taaaaacca 
      acttgaaaa ttttaaatt cactcagtg ttttggctg 
      aaatagctc tgaaaagtg agggttgtt tcgccctaa 
      tttggcaca atttcttca caaaggagg aggatttgt 
      ggcaaaacg acgtgaaaa tattaatta cgtttccaa 
      atcgaatct caaacgctc ttcaaggcc aattcaacc 
      aatttttca aaccaaacg agtattcaa aattcctgg 
      ttttcaacg taatcaaag tgattgaat tttcaaccg 
      acttgctac tccgggaac caaaatatg aaaaaaatt 
      tctattaca caaacctgg cattgtttt taagcaaaa 
      ctttgatta actacaacg aacattaca attccggtg 
      ctattaaaa ctttcgtaa aagttgcaa atattctga 
      ctaaccaaa agtcagaca aacttaatg tttatcgaa 
      cagtatttt ggaatttgt tcgaaagtt tggaacgga 
      gcgctgtag aaatcctag cagtgacag ttttgcaaa 
      atactgacc cttccgttt acacaatta aaagtgttc 
      taggcccca atttaatca aatgtgata actggaagt 
      acgttttgt gtacaaaaa tccccctgt tatcacatt 
      acgataacg agaatctca ttaaaaatt taatagtgt 
      atttaagtg ccacgtccg agcacaatt tgttcacac 
      ttttagtga acctaatca ctgtttggt ccgacaaat 
      cggccctca accacacga gtgtggaac accacttgt 
      atttaacaa aactaatcg ttgttataa taaacagtt 
      tcaaaaata ttggcctaa gtgagcacc agaggcaga 
      ttttgcagt caaaaatgt actcggggg tccccgtcc 
      acttggaga actcgaccc cttctgtga gggccatgg 
      gcatgaaag tgtcggaga ttgttttga attttatcc 
      aaactgaga gcgaaaaaa gcgaaactt tgagattcg 
      agttcgatc aaatgccca acgagggga aagaagttg 
      gcgaaattt tgcaaaaac tgttcgata cggacatga 
      cgatttact tcaagaata aagacgttg aagaggatg 
      ttttcccct tgatttcaa gtcttttag aggcggaaa 
      agtgccccg agtatgtgc ccccagcga ggcaattgc 
      gggggcaag aggagccca aaaatgtgg tcgtggctg 
      aagccatcc accaaaaaa ataatctgc tcacgattg 
      tagacagta ccgacttcg agttggact cagatgatg 
      atctggagc cttacgatt tggcaaacg atgtgaaag 
      tgtcgaaaa aggagcccc cgaagtact tgcgggact 
      tgagagacg gtttattgg agacgcaag attgcgacg 
      tttttagtt taagtttgg aagcgtgcg aggatttga 
      ttattaagc agctacccg acgatgatg tctcacttg 
      gggtcgagt tactgcaaa ttttggtcg gactagacc 
      aacgttttt acgttgaaa acttcgatt ctgtggtgt 
      ttaaaagct gcgttgcga taacttgtg tttatccat 
      cacaaagtg ctgaatatt tatgcaagt ggtttcatg 
      ccgacgttg ggacttatt cgatctctc accgcattt 
      tcctacttg atgttttaa gtgaagctt cgaggaatt 
      tgtcggctt taacgctgg acaagccgg atgaaaaaa 
      tcgtaatca agcgcagga agaaaaacc ctgacttgg 
      agaaggccg aagaaataa ttaaaaaac gcctcgagt 
      ccaaaactc ggtacttcc acaaacaca aggctttcc 
      gagtggaaa aactgaata agtttgcac aagtggccg 
      gccacttct tttttccgt tactgtacg gttataatg 
      gcaataaaa tgatttcgc aaattcccc aaaacgaca 
      gtgatttta ttctgttga ttcacttta ttcggaccc 
      tgtctgtta tcatgtgtt cctcgcaaa attgtccga 
      ttgaaccgc gaatggcca aagaagtgt tacatttct 
      cgtggtatt tgcgcttcc acaaggagg ttaaagtga 
      ggattgctg tcatcggtt tgataggag ctgcagtta 
      taaacacgc cgaaagaga ttttgatgt cggacttca 
      ccagcgaat tgctcgagt tcaggcttt ggttggggg 
      atttactga gcttgagcg tgacccgag gagagccga 
      attcggagt gtcggagtt tagcggcta gtactatgt 
      gcattattg atagtttct tgaaagttg acgatgaat 
      aaagctttt cgtactaac tgggtgttt cattgcact 
      aattacatt tgtttgctt ggcttgata gcgcggttt 
      gatgcaggt ccgttgcta acccccaca tcggcccaa 
      cagcccctt caaccgttg tcttccgca tcgtccaat 
      aacaacacc gaggttaaa acaccagca aataaaaac 
      caacagaat atcgggtcg acgtggagc aactcaggc 
      aacttaaat atcgattta gataattta ctcgtgacg 
      aaaccaaaa caaggagtc tcacctagt atgaaccaa 
      ttggcgagc aatccaacc tcacccgtt aatcaaccg 
      aaattaaca aatcaatat gcgatgaac ttcaacgct 
      caaggcttc ccccaaacg aatgctttc aatcagcca 
      aataagcaa ctgtttcca agcttttaa agcacctaa 
      tttttattc gaaattcca aagattgta ctttaatcg 
      ttgagattg agccaattg gtcttgcgt ttatagttt 
      ttaaaaacg gttttcttg cacatattt tgtatagtc 
      cgtttggtt gtattacaa tgctcatga gggtgctgg 
      gtacattta agtttgttc atattttat ttagtatta 
      cgcaactat ttatacaac taatttgta ggaacagca 
      aaatcggct ctgttagca aacagataa agcaatatt 
      gcattgtta ataattttc tataaaata aaaacaaat 
      tcgacctat cggcagttt tttcttttt cgttgaagg 
      ttgcgatac cgtggtgcc ttattatcg ataacacta 
      tccatgtta cagttagta actgtttga acattccgc 
      tcatttagt ttattgtaa aaacatata aaataaatt 
      gttgtgaat att
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