Status
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Gene information
General information
TC012154 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4047734..4050310
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012154
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01933

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012154 RA
      MLKTVSSKY PRISLVKKP KCNHYSTKL SYKHNLGEE 
      PLRHITIGT LLEETVAKF GDRKALISR HQKKSLTFA 
      EILDQTDKL ASGFRSIGL EKNDRVGLW APNLIEWYI 
      TKMACARAG LIMVGLNPA YQPPEMEYC INKVGIKAI 
      VCADQFKSQ NFYENLATV APELEKSEP GKLKSGKVP 
      SLQTVITMS ENKKRGAFN FGEILDLAT NDSIAQIRK 
      YQRDISPDG PCNLQFTSG TTGKPKAAL TSHFNMVNN 
      GYLIGKRNE LNRKHHTIC LQVPFFHAF GTTISIMAA 
      LNHGATMVL PTDGYSPDK SLDAIKEEK CTIIHGTPT 
      MYVDLVHQQ EVRKEDISP EIAVAGGAL CAPQLFKKM 
      LSVLNVKKV KSVYGLTET TAVVFQSMV TESEEKAVS 
      TVGHVGEHV EVKIVDKDG VVVPCGTPG ELCIRAYCN 
      MLGYWEDEA KTKEMIGPD NWLMTGDQF ILEEDGYGK 
      VVGRLKEMI IRGGENIFP KEIEEFLNS HPNILETHV 
      IGLPHERLG EEVCACIRV KPGTKVTFD DMVSFCKGK 
      MAHFKIPSK LKVVDEFPK TTSGKIQKF KLKDLPAN
    CDS sequence
    • >TC012154 RA
      atgttgaag actgttagc agcaaatat ccccggata 
      tctctagta aaaaaacca aaatgtaac cactactca 
      accaaacta agctacaaa cataacctt ggcgaagaa 
      ccattacgt cacattact atcggaaca cttctcgag 
      gaaaccgtc gctaaattt ggggacagg aaagcattg 
      atctctaga caccagaaa aagtcacta acttttgcg 
      gaaattctg gaccagacg gacaaacta gcatcaggg 
      tttaggtcg ataggatta gaaaaaaat gaccgggtg 
      ggcctatgg gcgcccaat ttaatcgaa tggtacatt 
      actaaaatg gcctgtgcg cgagctggg ttgattatg 
      gtaggctta aatccggcc taccagccg ccggaaatg 
      gagtattgt attaataag gtcggaatt aaggccatt 
      gtgtgcgct gatcagttc aagagccaa aatttttat 
      gaaaacttg gcaactgta gcgcccgaa ctggaaaaa 
      tccgagcct ggaaaactg aaaagtggc aaagtaccg 
      tcgcttcaa actgtgata accatgagt gaaaacaaa 
      aaacgaggg gccttcaat ttcggcgaa attctagac 
      ttagctacc aatgacagt attgcacaa atcagaaag 
      taccaacga gatattagt cccgacggc ccttgcaat 
      ttacagttt acatcggga accaccggt aaaccgaaa 
      gctgctctg acctctcac ttcaatatg gtcaacaat 
      ggctatctg ataggcaaa cgaaacgaa ctgaatcgg 
      aaacatcac accatttgc ttgcaagta ccatttttc 
      cacgctttc ggtaccacc atatccata atggcggcg 
      ttaaaccac ggcgcaacg atggtacta cccacagat 
      gggtacagt ccggacaaa tcccttgat gccatcaag 
      gaagagaaa tgtaccata atccacggt accccaacc 
      atgtacgtg gatctggtc caccagcaa gaagtgcgc 
      aaagaggac atctcgccg gaaatcgca gttgcaggt 
      ggggctctt tgtgcccct caacttttc aaaaaaatg 
      ctttcggtg ctcaatgtg aaaaaagtg aaatctgtc 
      tatggtttg actgaaacc acggcggta gtgttccag 
      tctatggtc acagaaagc gaagaaaag gctgtttca 
      accgttggc cacgttggt gaacatgtt gaggtgaaa 
      atcgttgac aaggacggt gttgtggtc ccgtgtggc 
      accccaggc gagttgtgc atacgggca tattgcaat 
      atgttgggt tattgggaa gatgaagcc aagaccaag 
      gaaatgatc ggacccgat aattggcta atgactggt 
      gaccagttt atacttgaa gaagacggt tacggaaaa 
      gtcgtcgga cgactcaaa gaaatgatt ataagggga 
      ggcgaaaac atattccct aaagaaatt gaagaattc 
      cttaattcc catccaaat attttggag acacatgtt 
      atcgggctt ccccacgaa cggcttgga gaggaagtt 
      tgtgcatgc attcgcgtc aaacccggg accaaagtc 
      acgttcgac gatatggtc agtttttgc aaaggcaaa 
      atggcccat tttaaaatc ccgtccaag ttgaaagtt 
      gttgacgaa ttccccaaa acaacttcg ggcaaaata 
      caaaagttc aaactgaag gacttgccc gcaaattaa
    mRNA sequence
    • >TC012154 RA
      tgcgtctag ttttgagcc aagttcagt attacgagt 
      atttatcaa ccagcaaca ttatggttt ttatcttgt 
      cgtttagca agtttagtg attatcttt taattatcg 
      ggtctaata gctaataca tttataccg cggtacttt 
      ttaatctaa ttatattcc tctagtaaa aatgttgaa 
      gactgttag cagcaaata tccccggat atctctagt 
      aaaaaaacc aaaatgtaa ccactactc aaccaaact 
      aagctacaa acataacct tggcgaaga accattacg 
      tcacattac tatcggaac acttctcga ggaaaccgt 
      cgctaaatt tggggacag gaaagcatt gatctctag 
      acaccagaa aaagtcact aacttttgc ggaaattct 
      ggaccagac ggacaaact agcatcagg gtttaggtc 
      gataggatt agaaaaaaa tgaccgggt gggcctatg 
      ggcgcccaa tttaatcga atggtacat tactaaaat 
      ggcctgtgc gcgagctgg gttgattat ggtaggctt 
      aaatccggc ctaccagcc gccggaaat ggagtattg 
      tattaataa ggtcggaat taaggccat tgtgtgcgc 
      tgatcagtt caagagcca aaattttta tgaaaactt 
      ggcaactgt agcgcccga actggaaaa atccgagcc 
      tggaaaact gaaaagtgg caaagtacc gtcgcttca 
      aactgtgat aaccatgag tgaaaacaa aaaacgagg 
      ggccttcaa tttcggcga aattctaga cttagctac 
      caatgacag tattgcaca aatcagaaa gtaccaacg 
      agatattag tcccgacgg cccttgcaa tttacagtt 
      tacatcggg aaccaccgg taaaccgaa agctgctct 
      gacctctca cttcaatat ggtcaacaa tggctatct 
      gataggcaa acgaaacga actgaatcg gaaacatca 
      caccatttg cttgcaagt accattttt ccacgcttt 
      cggtaccac catatccat aatggcggc gttaaacca 
      cggcgcaac gatggtact acccacaga tgggtacag 
      tccggacaa atcccttga tgccatcaa ggaagagaa 
      atgtaccat aatccacgg taccccaac catgtacgt 
      ggatctggt ccaccagca agaagtgcg caaagagga 
      catctcgcc ggaaatcgc agttgcagg tggggctct 
      ttgtgcccc tcaactttt caaaaaaat gctttcggt 
      gctcaatgt gaaaaaagt gaaatctgt ctatggttt 
      gactgaaac cacggcggt agtgttcca gtctatggt 
      cacagaaag cgaagaaaa ggctgtttc aaccgttgg 
      ccacgttgg tgaacatgt tgaggtgaa aatcgttga 
      caaggacgg tgttgtggt cccgtgtgg caccccagg 
      cgagttgtg catacgggc atattgcaa tatgttggg 
      ttattggga agatgaagc caagaccaa ggaaatgat 
      cggacccga taattggct aatgactgg tgaccagtt 
      tatacttga agaagacgg ttacggaaa agtcgtcgg 
      acgactcaa agaaatgat tataagggg aggcgaaaa 
      catattccc taaagaaat tgaagaatt ccttaattc 
      ccatccaaa tattttgga gacacatgt tatcgggct 
      tccccacga acggcttgg agaggaagt ttgtgcatg 
      cattcgcgt caaacccgg gaccaaagt cacgttcga 
      cgatatggt cagtttttg caaaggcaa aatggccca 
      ttttaaaat cccgtccaa gttgaaagt tgttgacga 
      attccccaa aacaacttc gggcaaaat acaaaagtt 
      caaactgaa ggacttgcc cgcaaatta aaacagtac 
      aaaaacgtg taattgaaa tatttattt tttaactgt 
      tgttttaat aaatttttc tcgcatag
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