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Gene information
General information
TC012153 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4079033..4081570
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012153
    iBeetle screen iB_01932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012153 RA
      MPVYTREFL SQIPSVDFC QADATKSDV FYYLFKSAT 
QTTAITINE DYSLPEDER NLPMKILMH GYTSNVTSP 
WYKQMKQEY FQKGPHNII YVDWSIASN KSFAVSAAN 
IKPVGEFIA DLIVSLRVP VENVHLIGH SLGSHLAGF 
VGKNIYSKT GKKVARITA TDAAGPGFE NAKPEARLN 
KHDATFIDV IHTDVNYYG ILKPIGHVD FYVNGGKNQ 
PGCPARKVD DNCSHARSN DYFIESINK RNVMARPAK 
LNDEEEIVL TSRTEDVEF GDWTPATAR GIYFIKTNK 
TAPYLLQKK IKDT
    • >TC012153 RB
      MTIASLIIF YTLLLYQGK LTSQLVYTR EFLSQIPSV 
DFCQADATK SDVFYYLFK SATQTTAIT INEDYSLPE 
DERNLPMKI LMHGYTSNV TSPWYKQMK QEYFQKGPH 
NIIYVDWSI ASNKSFAVS AANIKPVGE FIADLIVSL 
RVPVENVHL IGHSLGSHL AGFVGKNIY SKTGKKVAR 
ITATDAAGP GFENAKPEA RLNKHDATF IDVIHTDVN 
YYGILKPIG HVDFYVNGG KNQPGCPAR KVDDNCSHA 
RSNDYFIES INKRNVMAR PAKLNDEEE IVLTSRTED 
VEFGDWTPA TARGIYFIK TNKTAPYLL QKKIKDT
    CDS sequence
    • >TC012153 RA
      atgccagtt tacactcga gaattttta tcccaaatc 
ccttccgtc gacttctgt caagctgac gcgaccaaa 
tccgacgtc ttttactac ttattcaaa agtgcaacc 
caaacgact gctataaca ataaacgag gactactcg 
ttacccgaa gatgagaga aaccttccc atgaaaata 
ctaatgcat ggttacact tcaaacgta acatcaccg 
tggtacaaa caaatgaag caagagtat ttccaaaaa 
ggcccacat aacattatt tacgtcgac tggtccatt 
gcttcgaat aaaagcttc gcagtgtct gcagctaac 
attaaaccc gttggggaa tttatagcc gatttgata 
gtcagccta agggttccg gtggaaaac gtgcacctt 
attggacac tcactagga tcacatttg gccggtttc 
gtgggcaaa aatatttat tcaaaaacc gggaaaaag 
gtggcaagg atcacagca acggacgcc gctggtcca 
ggctttgaa aatgcgaaa cctgaagcc aggttgaac 
aaacacgac gctactttt atcgatgtg atacacact 
gatgtgaat tattacggc attttgaaa cccataggg 
catgtcgat ttttacgta aacggcggg aaaaaccaa 
cctgggtgc cctgcacgt aaagtcgat gataattgt 
agccatgcg aggtccaac gattatttc atcgaaagc 
ataaacaag agaaatgtt atggcaagg ccggcgaaa 
ttaaacgat gaagaagaa attgtgcta acaagccgg 
actgaagac gtggaattt ggggactgg acaccggca 
acagctaga gggatttat tttattaaa acaaataaa 
acggcacct tacttatta cagaaaaaa ataaaagat 
acgtaa
    • >TC012153 RB
      atgaccatt gcatcttta atcattttc tacacattg 
ctcctctac caaggtaaa ctaacttca cagttagtt 
tacactcga gaattttta tcccaaatc ccttccgtc 
gacttctgt caagctgac gcgaccaaa tccgacgtc 
ttttactac ttattcaaa agtgcaacc caaacgact 
gctataaca ataaacgag gactactcg ttacccgaa 
gatgagaga aaccttccc atgaaaata ctaatgcat 
ggttacact tcaaacgta acatcaccg tggtacaaa 
caaatgaag caagagtat ttccaaaaa ggcccacat 
aacattatt tacgtcgac tggtccatt gcttcgaat 
aaaagcttc gcagtgtct gcagctaac attaaaccc 
gttggggaa tttatagcc gatttgata gtcagccta 
agggttccg gtggaaaac gtgcacctt attggacac 
tcactagga tcacatttg gccggtttc gtgggcaaa 
aatatttat tcaaaaacc gggaaaaag gtggcaagg 
atcacagca acggacgcc gctggtcca ggctttgaa 
aatgcgaaa cctgaagcc aggttgaac aaacacgac 
gctactttt atcgatgtg atacacact gatgtgaat 
tattacggc attttgaaa cccataggg catgtcgat 
ttttacgta aacggcggg aaaaaccaa cctgggtgc 
cctgcacgt aaagtcgat gataattgt agccatgcg 
aggtccaac gattatttc atcgaaagc ataaacaag 
agaaatgtt atggcaagg ccggcgaaa ttaaacgat 
gaagaagaa attgtgcta acaagccgg actgaagac 
gtggaattt ggggactgg acaccggca acagctaga 
gggatttat tttattaaa acaaataaa acggcacct 
tacttatta cagaaaaaa ataaaagat acgtaa
    mRNA sequence
    • >TC012153 RA
      acagtcaac aaatctatc ccactcgaa aaataaaaa 
gtgtagtat accgtacag tatttttcg tcgtgaagt 
gagtgatct ttgaataat gccagttta cactcgaga 
atttttatc ccaaatccc ttccgtcga cttctgtca 
agctgacgc gaccaaatc cgacgtctt ttactactt 
attcaaaag tgcaaccca aacgactgc tataacaat 
aaacgagga ctactcgtt acccgaaga tgagagaaa 
ccttcccat gaaaatact aatgcatgg ttacacttc 
aaacgtaac atcaccgtg gtacaaaca aatgaagca 
agagtattt ccaaaaagg cccacataa cattattta 
cgtcgactg gtccattgc ttcgaataa aagcttcgc 
agtgtctgc agctaacat taaacccgt tggggaatt 
tatagccga tttgatagt cagcctaag ggttccggt 
ggaaaacgt gcaccttat tggacactc actaggatc 
acatttggc cggtttcgt gggcaaaaa tatttattc 
aaaaaccgg gaaaaaggt ggcaaggat cacagcaac 
ggacgccgc tggtccagg ctttgaaaa tgcgaaacc 
tgaagccag gttgaacaa acacgacgc tacttttat 
cgatgtgat acacactga tgtgaatta ttacggcat 
tttgaaacc catagggca tgtcgattt ttacgtaaa 
cggcgggaa aaaccaacc tgggtgccc tgcacgtaa 
agtcgatga taattgtag ccatgcgag gtccaacga 
ttatttcat cgaaagcat aaacaagag aaatgttat 
ggcaaggcc ggcgaaatt aaacgatga agaagaaat 
tgtgctaac aagccggac tgaagacgt ggaatttgg 
ggactggac accggcaac agctagagg gatttattt 
tattaaaac aaataaaac ggcacctta cttattaca 
gaaaaaaat aaaagatac gtaactaat ctgtgtttt 
atttggttt tgtttactg tgtcaacga
    • >TC012153 RB
      accaataac aaaatctcc tttatgtca catttcctg 
tcacatatc tctaaatta ataaagttt aaattctgt 
aacaatgac cattgcatc tttaatcat tttctacac 
attgctcct ctaccaagg taaactaac ttcacagtt 
agtttacac tcgagaatt tttatccca aatcccttc 
cgtcgactt ctgtcaagc tgacgcgac caaatccga 
cgtctttta ctacttatt caaaagtgc aacccaaac 
gactgctat aacaataaa cgaggacta ctcgttacc 
cgaagatga gagaaacct tcccatgaa aatactaat 
gcatggtta cacttcaaa cgtaacatc accgtggta 
caaacaaat gaagcaaga gtatttcca aaaaggccc 
acataacat tatttacgt cgactggtc cattgcttc 
gaataaaag cttcgcagt gtctgcagc taacattaa 
acccgttgg ggaatttat agccgattt gatagtcag 
cctaagggt tccggtgga aaacgtgca ccttattgg 
acactcact aggatcaca tttggccgg tttcgtggg 
caaaaatat ttattcaaa aaccgggaa aaaggtggc 
aaggatcac agcaacgga cgccgctgg tccaggctt 
tgaaaatgc gaaacctga agccaggtt gaacaaaca 
cgacgctac ttttatcga tgtgataca cactgatgt 
gaattatta cggcatttt gaaacccat agggcatgt 
cgattttta cgtaaacgg cgggaaaaa ccaacctgg 
gtgccctgc acgtaaagt cgatgataa ttgtagcca 
tgcgaggtc caacgatta tttcatcga aagcataaa 
caagagaaa tgttatggc aaggccggc gaaattaaa 
cgatgaaga agaaattgt gctaacaag ccggactga 
agacgtgga atttgggga ctggacacc ggcaacagc 
tagagggat ttattttat taaaacaaa taaaacggc 
accttactt attacagaa aaaaataaa agatacgta 
actaatctg tgttttatt tggttttgt ttactgtgt 
caacga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY