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Gene information
General information
TC012153 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4079033..4081570
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012153
    iBeetle screen iB_01932

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012153 RA
      MPVYTREFL SQIPSVDFC QADATKSDV FYYLFKSAT 
      QTTAITINE DYSLPEDER NLPMKILMH GYTSNVTSP 
      WYKQMKQEY FQKGPHNII YVDWSIASN KSFAVSAAN 
      IKPVGEFIA DLIVSLRVP VENVHLIGH SLGSHLAGF 
      VGKNIYSKT GKKVARITA TDAAGPGFE NAKPEARLN 
      KHDATFIDV IHTDVNYYG ILKPIGHVD FYVNGGKNQ 
      PGCPARKVD DNCSHARSN DYFIESINK RNVMARPAK 
      LNDEEEIVL TSRTEDVEF GDWTPATAR GIYFIKTNK 
      TAPYLLQKK IKDT
    • >TC012153 RB
      MTIASLIIF YTLLLYQGK LTSQLVYTR EFLSQIPSV 
      DFCQADATK SDVFYYLFK SATQTTAIT INEDYSLPE 
      DERNLPMKI LMHGYTSNV TSPWYKQMK QEYFQKGPH 
      NIIYVDWSI ASNKSFAVS AANIKPVGE FIADLIVSL 
      RVPVENVHL IGHSLGSHL AGFVGKNIY SKTGKKVAR 
      ITATDAAGP GFENAKPEA RLNKHDATF IDVIHTDVN 
      YYGILKPIG HVDFYVNGG KNQPGCPAR KVDDNCSHA 
      RSNDYFIES INKRNVMAR PAKLNDEEE IVLTSRTED 
      VEFGDWTPA TARGIYFIK TNKTAPYLL QKKIKDT
    CDS sequence
    • >TC012153 RA
      atgccagtt tacactcga gaattttta tcccaaatc 
      ccttccgtc gacttctgt caagctgac gcgaccaaa 
      tccgacgtc ttttactac ttattcaaa agtgcaacc 
      caaacgact gctataaca ataaacgag gactactcg 
      ttacccgaa gatgagaga aaccttccc atgaaaata 
      ctaatgcat ggttacact tcaaacgta acatcaccg 
      tggtacaaa caaatgaag caagagtat ttccaaaaa 
      ggcccacat aacattatt tacgtcgac tggtccatt 
      gcttcgaat aaaagcttc gcagtgtct gcagctaac 
      attaaaccc gttggggaa tttatagcc gatttgata 
      gtcagccta agggttccg gtggaaaac gtgcacctt 
      attggacac tcactagga tcacatttg gccggtttc 
      gtgggcaaa aatatttat tcaaaaacc gggaaaaag 
      gtggcaagg atcacagca acggacgcc gctggtcca 
      ggctttgaa aatgcgaaa cctgaagcc aggttgaac 
      aaacacgac gctactttt atcgatgtg atacacact 
      gatgtgaat tattacggc attttgaaa cccataggg 
      catgtcgat ttttacgta aacggcggg aaaaaccaa 
      cctgggtgc cctgcacgt aaagtcgat gataattgt 
      agccatgcg aggtccaac gattatttc atcgaaagc 
      ataaacaag agaaatgtt atggcaagg ccggcgaaa 
      ttaaacgat gaagaagaa attgtgcta acaagccgg 
      actgaagac gtggaattt ggggactgg acaccggca 
      acagctaga gggatttat tttattaaa acaaataaa 
      acggcacct tacttatta cagaaaaaa ataaaagat 
      acgtaa
    • >TC012153 RB
      atgaccatt gcatcttta atcattttc tacacattg 
      ctcctctac caaggtaaa ctaacttca cagttagtt 
      tacactcga gaattttta tcccaaatc ccttccgtc 
      gacttctgt caagctgac gcgaccaaa tccgacgtc 
      ttttactac ttattcaaa agtgcaacc caaacgact 
      gctataaca ataaacgag gactactcg ttacccgaa 
      gatgagaga aaccttccc atgaaaata ctaatgcat 
      ggttacact tcaaacgta acatcaccg tggtacaaa 
      caaatgaag caagagtat ttccaaaaa ggcccacat 
      aacattatt tacgtcgac tggtccatt gcttcgaat 
      aaaagcttc gcagtgtct gcagctaac attaaaccc 
      gttggggaa tttatagcc gatttgata gtcagccta 
      agggttccg gtggaaaac gtgcacctt attggacac 
      tcactagga tcacatttg gccggtttc gtgggcaaa 
      aatatttat tcaaaaacc gggaaaaag gtggcaagg 
      atcacagca acggacgcc gctggtcca ggctttgaa 
      aatgcgaaa cctgaagcc aggttgaac aaacacgac 
      gctactttt atcgatgtg atacacact gatgtgaat 
      tattacggc attttgaaa cccataggg catgtcgat 
      ttttacgta aacggcggg aaaaaccaa cctgggtgc 
      cctgcacgt aaagtcgat gataattgt agccatgcg 
      aggtccaac gattatttc atcgaaagc ataaacaag 
      agaaatgtt atggcaagg ccggcgaaa ttaaacgat 
      gaagaagaa attgtgcta acaagccgg actgaagac 
      gtggaattt ggggactgg acaccggca acagctaga 
      gggatttat tttattaaa acaaataaa acggcacct 
      tacttatta cagaaaaaa ataaaagat acgtaa
    mRNA sequence
    • >TC012153 RA
      acagtcaac aaatctatc ccactcgaa aaataaaaa 
      gtgtagtat accgtacag tatttttcg tcgtgaagt 
      gagtgatct ttgaataat gccagttta cactcgaga 
      atttttatc ccaaatccc ttccgtcga cttctgtca 
      agctgacgc gaccaaatc cgacgtctt ttactactt 
      attcaaaag tgcaaccca aacgactgc tataacaat 
      aaacgagga ctactcgtt acccgaaga tgagagaaa 
      ccttcccat gaaaatact aatgcatgg ttacacttc 
      aaacgtaac atcaccgtg gtacaaaca aatgaagca 
      agagtattt ccaaaaagg cccacataa cattattta 
      cgtcgactg gtccattgc ttcgaataa aagcttcgc 
      agtgtctgc agctaacat taaacccgt tggggaatt 
      tatagccga tttgatagt cagcctaag ggttccggt 
      ggaaaacgt gcaccttat tggacactc actaggatc 
      acatttggc cggtttcgt gggcaaaaa tatttattc 
      aaaaaccgg gaaaaaggt ggcaaggat cacagcaac 
      ggacgccgc tggtccagg ctttgaaaa tgcgaaacc 
      tgaagccag gttgaacaa acacgacgc tacttttat 
      cgatgtgat acacactga tgtgaatta ttacggcat 
      tttgaaacc catagggca tgtcgattt ttacgtaaa 
      cggcgggaa aaaccaacc tgggtgccc tgcacgtaa 
      agtcgatga taattgtag ccatgcgag gtccaacga 
      ttatttcat cgaaagcat aaacaagag aaatgttat 
      ggcaaggcc ggcgaaatt aaacgatga agaagaaat 
      tgtgctaac aagccggac tgaagacgt ggaatttgg 
      ggactggac accggcaac agctagagg gatttattt 
      tattaaaac aaataaaac ggcacctta cttattaca 
      gaaaaaaat aaaagatac gtaactaat ctgtgtttt 
      atttggttt tgtttactg tgtcaacga
    • >TC012153 RB
      accaataac aaaatctcc tttatgtca catttcctg 
      tcacatatc tctaaatta ataaagttt aaattctgt 
      aacaatgac cattgcatc tttaatcat tttctacac 
      attgctcct ctaccaagg taaactaac ttcacagtt 
      agtttacac tcgagaatt tttatccca aatcccttc 
      cgtcgactt ctgtcaagc tgacgcgac caaatccga 
      cgtctttta ctacttatt caaaagtgc aacccaaac 
      gactgctat aacaataaa cgaggacta ctcgttacc 
      cgaagatga gagaaacct tcccatgaa aatactaat 
      gcatggtta cacttcaaa cgtaacatc accgtggta 
      caaacaaat gaagcaaga gtatttcca aaaaggccc 
      acataacat tatttacgt cgactggtc cattgcttc 
      gaataaaag cttcgcagt gtctgcagc taacattaa 
      acccgttgg ggaatttat agccgattt gatagtcag 
      cctaagggt tccggtgga aaacgtgca ccttattgg 
      acactcact aggatcaca tttggccgg tttcgtggg 
      caaaaatat ttattcaaa aaccgggaa aaaggtggc 
      aaggatcac agcaacgga cgccgctgg tccaggctt 
      tgaaaatgc gaaacctga agccaggtt gaacaaaca 
      cgacgctac ttttatcga tgtgataca cactgatgt 
      gaattatta cggcatttt gaaacccat agggcatgt 
      cgattttta cgtaaacgg cgggaaaaa ccaacctgg 
      gtgccctgc acgtaaagt cgatgataa ttgtagcca 
      tgcgaggtc caacgatta tttcatcga aagcataaa 
      caagagaaa tgttatggc aaggccggc gaaattaaa 
      cgatgaaga agaaattgt gctaacaag ccggactga 
      agacgtgga atttgggga ctggacacc ggcaacagc 
      tagagggat ttattttat taaaacaaa taaaacggc 
      accttactt attacagaa aaaaataaa agatacgta 
      actaatctg tgttttatt tggttttgt ttactgtgt 
      caacga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY