Status
Please wait ...
Gene information
General information
TC012134 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5016919..5027416
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012134
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01931

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    vitellogenic egg chamber late sometimesnumber increased - Number of animals showing the phenotype: 1

    ovary size decreased - Number of animals showing the phenotype: 3

    ovariole sometimes not present - Number of animals showing the phenotype: 3

    tropharium size decreased - Number of animals showing the phenotype: 3

    egg chamber mostly not present - Number of animals showing the phenotype: 3

    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012134 RA
      MLLPVLFSL IAYLNIFVI FLFVVKRMD LFNLFYGSL 
      QLTIVLFFV YFILLLKWV NKMVDIVTN RIFDSFSRF 
      SPKENHQTE SLTTISHLK RFSKGNELR RRNFLKDDL 
      KERDSHLYD IKENVAEVE LPPSRPLMG LSCDYCTSA 
      SRESLVNKT TEKFALNNI LDHTPSLDK NGFLQRTFS 
      HLNQVYQLR KFDYPQVAE IVLKDDRLK KAIEKTALQ 
      QYQDSERND DEFYQELLK NNHKRAKKL LYGMRSTLS 
      DFLLRFTSW VLYKLLPCF LSCVVAHPG QINMLKEAG 
      KTSLPLIFL PLHRSHLDY ILISFILLN NNVRSPLVA 
      AGDNLRIPF FGSLLRGLG AFFIKRRID PVMGRKDHL 
      YKAVLHTYM NECLRAGHN MEFFLEGGR TRTGKPCMP 
      KYGILSVIV EAFMDGTIE DALLVPVSV NYEKLVDGN 
      FVREQLGQP KEMETFGAA IKGIWHVLN SDYGMMRID 
      FNQPFSLRE LVKTFNASK KLQNGFKKM LKSNPSTTS 
      LYGTDVVSD EHKSVVESI SKHIIYDCA RSTSVMSTN 
      ALAFLLLTK FREGATIEQ LVAALDSLR HELDVARKD 
      LGFTGDSID VVNYAIELL GPGLVRKEK INGEDIIKP 
      MAILPNVIE LTYYSNTLV THYALESIV AISLNMVAT 
      KAGTVSHLE LIETVLDLC NVLQYEFIL CKPCQNLEH 
      VVIGCLDDL NVKYRIFVE EVRDDEQVA RSKKIAQEF 
      EDEDLDEAA TEKPYVLSN EKGAVERLR FMQGLLMPL 
      VETYAVSAF TLEKLVGRS LLENELISD ILSEIKLQI 
      AEGTVKYDE SVSVDPIKN ALKLYQKWD VLECHTENK 
      LRLYYLKES QDNSDAVKT LYQRINKFR QSV
    CDS sequence
    • >TC012134 RA
      atgcttctc cctgtcctg ttcagtctc atcgcgtat 
      ttaaatatt ttcgttatt tttctgttc gtcgtgaag 
      aggatggat ctgtttaat ttattctac gggtcgttg 
      cagttgacc attgttttg tttttcgtc tattttatc 
      ttgctgctg aaatgggtg aacaaaatg gtggacatt 
      gtgaccaat cgaatcttc gatagcttt agtcgtttc 
      tcgcccaaa gaaaaccac cagactgaa tctctcaca 
      accatttca cacctgaaa cggttttcc aaaggtaac 
      gaactccgg aggaggaat tttcttaag gatgatctt 
      aaggagcgc gacagccac ctttacgac attaaggag 
      aatgtcgca gaagtcgag ctgccgccc agccggccc 
      ttgatgggc ctgagctgc gattactgc accagtgcc 
      agcagggaa tcgttggtc aataagaca accgaaaag 
      ttcgcgttg aacaacatt ttggaccat acgccctcg 
      ctggacaag aatgggttc ctgcaacgg actttctcc 
      cacttgaac caggtgtat cagctgagg aaatttgac 
      tacccacaa gtggctgaa atcgtcctg aaggacgac 
      cggctgaaa aaggccatc gagaagacg gcgttgcag 
      cagtaccaa gattccgag cggaacgat gatgagttt 
      taccaagaa ctgctgaaa aataatcat aaaagagcc 
      aagaaatta ttgtacggg atgaggtcc acactttct 
      gattttcta ctaagattt acgtcgtgg gttttgtac 
      aagttgttg ccgtgcttt ttgagctgt gtggttgca 
      catcctgga cagataaat atgctgaaa gaggcagga 
      aaaactagt cttcccttg atattcctt ccgctgcat 
      aggtcacat ttagattat attttaatt tcttttatt 
      ttgttgaat aataatgta cggtcacct ctcgtggca 
      gctggcgat aatttgagg ataccgttt tttgggtcc 
      ctcttgcgc gggctgggc gccttcttc ataaagcgc 
      cgcatcgac ccggtcatg ggccgtaaa gaccacctc 
      tacaaggcc gtcttacac acatacatg aacgaatgt 
      ctacgagcc ggccataac atggaattt ttcctcgaa 
      ggcggccgt acaagaacc ggcaaacct tgtatgccc 
      aagtacgga attcttagt gtaattgtt gaagcgttc 
      atggatggt accatcgag gatgcttta ttagttcca 
      gtcagtgtc aattacgag aaattagtc gatggaaat 
      ttcgtacgc gaacaatta ggacaaccc aaagaaatg 
      gaaacattc ggagctgcc attaagggc atctggcac 
      gtgctaaac agcgactat ggcatgatg cgcatcgac 
      tttaaccaa cccttctca ctcagggag ttggtgaag 
      acgttcaac gcctcgaag aagttacaa aacgggttc 
      aagaaaatg ctcaaatcg aatccctcg accaccagt 
      ctgtatggc acggacgtg gtgtcagac gaacacaag 
      agtgtagtc gagagtatt tccaaacac attatttat 
      gattgtgcc agatcgacg tcagtgatg tccacaaac 
      gccttagca ttcctcctg ttgaccaaa ttccgcgag 
      ggcgccact atcgaacaa ctagtggca gcgctagat 
      tccctccga catgaacta gatgtcgcc agaaaagat 
      ttgggtttc accggcgat tcaattgat gttgtcaac 
      tatgcgata gaattacta gggccagga ttggtccgc 
      aaggagaaa atcaacggc gaagacata attaaacca 
      atggcaatc ctcccgaac gtcatagaa ctgacttat 
      tacagcaac actttagtc acgcattac gcactcgag 
      tcgatcgtt gcaatctcg ctgaatatg gtggccacc 
      aaagcgggc accgtgtcc cacttggag ttaatcgaa 
      acagtactc gatttgtgc aacgtgctg cagtacgag 
      tttatattg tgcaagcct tgtcagaat ttggagcac 
      gtggtgatc gggtgtttg gacgatttg aacgtgaag 
      taccggatt ttcgtggag gaggtgcgg gacgatgag 
      caggtggcg aggagtaag aagatcgcg caggagttt 
      gaggacgag gatttggac gaggctgcg actgaaaag 
      ccgtacgtt ttgagcaac gaaaagggg gctgtggag 
      aggctgagg ttcatgcag gggttgcta atgccgttg 
      gtggagacg tacgcagtg tctgcgttt acgttggag 
      aagttggtt gggaggtct ctgcttgag aacgaactg 
      attagcgat attttaagt gagattaag ctgcaaata 
      gcggagggg acggtgaaa tatgatgag agtgtgtcc 
      gtggacccg atcaagaac gcgctgaag ctgtaccag 
      aagtgggac gttctggag tgccatacc gagaataaa 
      ctaagactt tattatctg aaggaaagc caagacaat 
      tccgacgct gtcaagacg ctgtatcaa aggatcaac 
      aaatttaga caatctgtt tag
    mRNA sequence
    • >TC012134 RA
      aaaacggta cattgagcc gcgctcccc caagcattc 
      agcgactac cggtgaaag aaacagaat ttcattgtc 
      gattagtac gtgcgtaag agagcttta cccccgagt 
      aagaatggg ctatatgtc tcgattgtg ctaaatata 
      ttcactaaa gtaacgtcg gctcggtgc gtggtcgga 
      tttttaatt gtcggatgt gattttgcg gtggtgcca 
      tggaggggg ttatcgagt gcgtcattc tgatggggt 
      tcatgtact gggccttcc ccagctttt tttcagagg 
      ggttctcca ggtaaaatt tgtaacaag ttgtgaaac 
      aataaaatc gtcagtgtg tcgatcgaa gtcgtggtg 
      acctgacgc gttcaaaaa tgcttctcc ctgtcctgt 
      tcagtctca tcgcgtatt taaatattt tcgttattt 
      ttctgttcg tcgtgaaga ggatggatc tgtttaatt 
      tattctacg ggtcgttgc agttgacca ttgttttgt 
      ttttcgtct attttatct tgctgctga aatgggtga 
      acaaaatgg tggacattg tgaccaatc gaatcttcg 
      atagcttta gtcgtttct cgcccaaag aaaaccacc 
      agactgaat ctctcacaa ccatttcac acctgaaac 
      ggttttcca aaggtaacg aactccgga ggaggaatt 
      ttcttaagg atgatctta aggagcgcg acagccacc 
      tttacgaca ttaaggaga atgtcgcag aagtcgagc 
      tgccgccca gccggccct tgatgggcc tgagctgcg 
      attactgca ccagtgcca gcagggaat cgttggtca 
      ataagacaa ccgaaaagt tcgcgttga acaacattt 
      tggaccata cgccctcgc tggacaaga atgggttcc 
      tgcaacgga ctttctccc acttgaacc aggtgtatc 
      agctgagga aatttgact acccacaag tggctgaaa 
      tcgtcctga aggacgacc ggctgaaaa aggccatcg 
      agaagacgg cgttgcagc agtaccaag attccgagc 
      ggaacgatg atgagtttt accaagaac tgctgaaaa 
      ataatcata aaagagcca agaaattat tgtacggga 
      tgaggtcca cactttctg attttctac taagattta 
      cgtcgtggg ttttgtaca agttgttgc cgtgctttt 
      tgagctgtg tggttgcac atcctggac agataaata 
      tgctgaaag aggcaggaa aaactagtc ttcccttga 
      tattccttc cgctgcata ggtcacatt tagattata 
      ttttaattt cttttattt tgttgaata ataatgtac 
      ggtcacctc tcgtggcag ctggcgata atttgagga 
      taccgtttt ttgggtccc tcttgcgcg ggctgggcg 
      ccttcttca taaagcgcc gcatcgacc cggtcatgg 
      gccgtaaag accacctct acaaggccg tcttacaca 
      catacatga acgaatgtc tacgagccg gccataaca 
      tggaatttt tcctcgaag gcggccgta caagaaccg 
      gcaaacctt gtatgccca agtacggaa ttcttagtg 
      taattgttg aagcgttca tggatggta ccatcgagg 
      atgctttat tagttccag tcagtgtca attacgaga 
      aattagtcg atggaaatt tcgtacgcg aacaattag 
      gacaaccca aagaaatgg aaacattcg gagctgcca 
      ttaagggca tctggcacg tgctaaaca gcgactatg 
      gcatgatgc gcatcgact ttaaccaac ccttctcac 
      tcagggagt tggtgaaga cgttcaacg cctcgaaga 
      agttacaaa acgggttca agaaaatgc tcaaatcga 
      atccctcga ccaccagtc tgtatggca cggacgtgg 
      tgtcagacg aacacaaga gtgtagtcg agagtattt 
      ccaaacaca ttatttatg attgtgcca gatcgacgt 
      cagtgatgt ccacaaacg ccttagcat tcctcctgt 
      tgaccaaat tccgcgagg gcgccacta tcgaacaac 
      tagtggcag cgctagatt ccctccgac atgaactag 
      atgtcgcca gaaaagatt tgggtttca ccggcgatt 
      caattgatg ttgtcaact atgcgatag aattactag 
      ggccaggat tggtccgca aggagaaaa tcaacggcg 
      aagacataa ttaaaccaa tggcaatcc tcccgaacg 
      tcatagaac tgacttatt acagcaaca ctttagtca 
      cgcattacg cactcgagt cgatcgttg caatctcgc 
      tgaatatgg tggccacca aagcgggca ccgtgtccc 
      acttggagt taatcgaaa cagtactcg atttgtgca 
      acgtgctgc agtacgagt ttatattgt gcaagcctt 
      gtcagaatt tggagcacg tggtgatcg ggtgtttgg 
      acgatttga acgtgaagt accggattt tcgtggagg 
      aggtgcggg acgatgagc aggtggcga ggagtaaga 
      agatcgcgc aggagtttg aggacgagg atttggacg 
      aggctgcga ctgaaaagc cgtacgttt tgagcaacg 
      aaaaggggg ctgtggaga ggctgaggt tcatgcagg 
      ggttgctaa tgccgttgg tggagacgt acgcagtgt 
      ctgcgttta cgttggaga agttggttg ggaggtctc 
      tgcttgaga acgaactga ttagcgata ttttaagtg 
      agattaagc tgcaaatag cggagggga cggtgaaat 
      atgatgaga gtgtgtccg tggacccga tcaagaacg 
      cgctgaagc tgtaccaga agtgggacg ttctggagt 
      gccataccg agaataaac taagacttt attatctga 
      aggaaagcc aagacaatt ccgacgctg tcaagacgc 
      tgtatcaaa ggatcaaca aatttagac aatctgttt 
      aggtatacc aaagtgttg ttattta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY