Status
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Gene information
General information
TC012126 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5187632..5189384
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012126
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01930

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012126 RA
      MNGPLKMYR TRQVVRHDI AVQLPDCMY KLKNIHAAR 
      NGMVEQRTD HVSSAPKKV DICDQNNQN LPEMAALEL 
      RQEEILRQL ADLKKQMDN IRNNLQISK NAAVTSQII 
      KPKQGPPPS LPDVIINAS PTNPPYSLE LVQKLWKNY 
      MFLSVKSYT HSSLSSLTE NAQQLTKTL ESFKPGNVP 
      KINVKLIWK DVGANPELV VAHSRIFGE VNILRYLAR 
      VTSDYLNYE TCNNVHEID ALLDVCYNI VKSKTKTER 
      ASLLQVLNK SLGKSQWLG DRSQISIAD LAAYSAIKQ 
      ATNPSEVNV NLGKWMQRC VACQ
    • >TC012126 RB
      MNGPLKMYR TRQVVRHDI AVQLPDCMY KLKNIHAAR 
      NGMVEQRTD HVSSAPKKV DICDQVKQF LKNNQNLPE 
      MAALELRQE EILRQLADL KKQMDNIRN NLQISKNAA 
      VTSQIIKPK QGPPPSLPD VIINASPTN PPYSLELVQ 
      KLWKNYMFL SVKSYTHSS LSSLTENAQ QLTKTLESF 
      KPGNVPKIN VKLIWKDVG ANPELVVAH SRIFGEVNI 
      LRYLARVTS DYLNYETCN NVHEIDALL DVCYNIVKS 
      KTKTERASL LQVLNKSLG KSQWLGDRS QISIADLAA 
      YSAIKQATN PSEVNVNLG KWMQRCVAC Q
    CDS sequence
    • >TC012126 RA
      atgaacggc ccgttgaag atgtaccgc acgagacag 
      gtcgttcgc cacgacatt gcagtgcag ctccctgac 
      tgtatgtac aaattgaaa aatatccac gctgcaaga 
      aatggtatg gttgaacaa cgaacagat cacgtttca 
      tcggccccg aaaaaagtg gatatttgc gaccagaat 
      aaccaaaat ttgcctgag atggctgcg ttggagctg 
      aggcaagag gaaatcctc cggcaactt gctgatttg 
      aagaaacaa atggacaac atcaggaac aacttacaa 
      atttccaaa aacgcggcc gttacgagc caaatcatc 
      aaacccaaa caaggacct cccccgagt ctccccgac 
      gtcataatc aacgcgagt ccaacaaac ccgccttat 
      tcgctagaa ctcgtccag aagttgtgg aaaaactac 
      atgtttcta tctgtaaaa agttacacc cattctagc 
      ctaagttcc ttgactgaa aatgcgcaa caattgacc 
      aaaaccttg gaaagtttc aagccggga aacgtcccg 
      aaaatcaac gtaaagctc atttggaaa gatgttgga 
      gctaatccg gaactggtg gtcgcacac tcgcgcatt 
      tttggcgaa gtcaatatt ctgaggtac ttagctagg 
      gtcacttcg gactacttg aactacgag acttgtaat 
      aatgttcac gaaatcgat gcgttactt gatgtttgt 
      tacaatatt gtaaagtct aagaccaag actgaaagg 
      gcgagtttg ttgcaagtt ttgaacaag tcgttgggg 
      aagagtcag tggttgggt gacaggagt caaatcagt 
      attgccgac ttagccgcc tactcagcc atcaaacag 
      gcgaccaat cctagtgaa gttaatgtt aatttagga 
      aagtggatg caaagatgt gtagcttgt caataa
    • >TC012126 RB
      atgaacggc ccgttgaag atgtaccgc acgagacag 
      gtcgttcgc cacgacatt gcagtgcag ctccctgac 
      tgtatgtac aaattgaaa aatatccac gctgcaaga 
      aatggtatg gttgaacaa cgaacagat cacgtttca 
      tcggccccg aaaaaagtg gatatttgc gaccaggtt 
      aaacagttt ttaaagaat aaccaaaat ttgcctgag 
      atggctgcg ttggagctg aggcaagag gaaatcctc 
      cggcaactt gctgatttg aagaaacaa atggacaac 
      atcaggaac aacttacaa atttccaaa aacgcggcc 
      gttacgagc caaatcatc aaacccaaa caaggacct 
      cccccgagt ctccccgac gtcataatc aacgcgagt 
      ccaacaaac ccgccttat tcgctagaa ctcgtccag 
      aagttgtgg aaaaactac atgtttcta tctgtaaaa 
      agttacacc cattctagc ctaagttcc ttgactgaa 
      aatgcgcaa caattgacc aaaaccttg gaaagtttc 
      aagccggga aacgtcccg aaaatcaac gtaaagctc 
      atttggaaa gatgttgga gctaatccg gaactggtg 
      gtcgcacac tcgcgcatt tttggcgaa gtcaatatt 
      ctgaggtac ttagctagg gtcacttcg gactacttg 
      aactacgag acttgtaat aatgttcac gaaatcgat 
      gcgttactt gatgtttgt tacaatatt gtaaagtct 
      aagaccaag actgaaagg gcgagtttg ttgcaagtt 
      ttgaacaag tcgttgggg aagagtcag tggttgggt 
      gacaggagt caaatcagt attgccgac ttagccgcc 
      tactcagcc atcaaacag gcgaccaat cctagtgaa 
      gttaatgtt aatttagga aagtggatg caaagatgt 
      gtagcttgt caataa
    mRNA sequence
    • >TC012126 RA
      aaacacttc gaatttttt cacattttc aaaatgaca 
      acgccagtt ccggtcaac cggaaacac caccgacaa 
      cattcgaca agatggcca cgcacccag cataggcga 
      ctcgtcact tatcgattt taaccgcca cgggcccca 
      ataacgctc tcaccgccc ggatttgac tacagttaa 
      gtgtctcac tgtcgcata tatcgccca aacgtgggt 
      aaatggtcg cagcttggc tttgtgcgc ctggtttgg 
      tgtgtgggt gacgtgcgc ccctgcttg acacttgcc 
      cccttactg tccgaacga ttttaataa aaacgagtc 
      gatattttg ttaaaattg cgaatttgt tggtaaaat 
      gaacggccc gttgaagat gtaccgcac gagacaggt 
      cgttcgcca cgacattgc agtgcagct ccctgactg 
      tatgtacaa attgaaaaa tatccacgc tgcaagaaa 
      tggtatggt tgaacaacg aacagatca cgtttcatc 
      ggccccgaa aaaagtgga tatttgcga ccagaataa 
      ccaaaattt gcctgagat ggctgcgtt ggagctgag 
      gcaagagga aatcctccg gcaacttgc tgatttgaa 
      gaaacaaat ggacaacat caggaacaa cttacaaat 
      ttccaaaaa cgcggccgt tacgagcca aatcatcaa 
      acccaaaca aggacctcc cccgagtct ccccgacgt 
      cataatcaa cgcgagtcc aacaaaccc gccttattc 
      gctagaact cgtccagaa gttgtggaa aaactacat 
      gtttctatc tgtaaaaag ttacaccca ttctagcct 
      aagttcctt gactgaaaa tgcgcaaca attgaccaa 
      aaccttgga aagtttcaa gccgggaaa cgtcccgaa 
      aatcaacgt aaagctcat ttggaaaga tgttggagc 
      taatccgga actggtggt cgcacactc gcgcatttt 
      tggcgaagt caatattct gaggtactt agctagggt 
      cacttcgga ctacttgaa ctacgagac ttgtaataa 
      tgttcacga aatcgatgc gttacttga tgtttgtta 
      caatattgt aaagtctaa gaccaagac tgaaagggc 
      gagtttgtt gcaagtttt gaacaagtc gttggggaa 
      gagtcagtg gttgggtga caggagtca aatcagtat 
      tgccgactt agccgccta ctcagccat caaacaggc 
      gaccaatcc tagtgaagt taatgttaa tttaggaaa 
      gtggatgca aagatgtgt agcttgtca ataaaacgc 
      ttgtttaaa ttagaggat cacagattt atttaaaat 
      tttgttaca cttaattac attaaactt aggcagtgt 
      aa
    • >TC012126 RB
      aaacacttc gaatttttt cacattttc aaaatgaca 
      acgccagtt ccggtcaac cggaaacac caccgacaa 
      cattcgaca agatggcca cgcacccag cataggcga 
      ctcgtcact tatcgattt taaccgcca cgggcccca 
      ataacgctc tcaccgccc ggatttgac tacagttaa 
      gtgtctcac tgtcgcata tatcgccca aacgtgggt 
      aaatggtcg cagcttggc tttgtgcgc ctggtttgg 
      tgtgtgggt gacgtgcgc ccctgcttg acacttgcc 
      cccttactg tccgaacga ttttaataa aaacgagtc 
      gatattttg ttaaaattg cgaatttgt tggtaaaat 
      gaacggccc gttgaagat gtaccgcac gagacaggt 
      cgttcgcca cgacattgc agtgcagct ccctgactg 
      tatgtacaa attgaaaaa tatccacgc tgcaagaaa 
      tggtatggt tgaacaacg aacagatca cgtttcatc 
      ggccccgaa aaaagtgga tatttgcga ccaggttaa 
      acagttttt aaagaataa ccaaaattt gcctgagat 
      ggctgcgtt ggagctgag gcaagagga aatcctccg 
      gcaacttgc tgatttgaa gaaacaaat ggacaacat 
      caggaacaa cttacaaat ttccaaaaa cgcggccgt 
      tacgagcca aatcatcaa acccaaaca aggacctcc 
      cccgagtct ccccgacgt cataatcaa cgcgagtcc 
      aacaaaccc gccttattc gctagaact cgtccagaa 
      gttgtggaa aaactacat gtttctatc tgtaaaaag 
      ttacaccca ttctagcct aagttcctt gactgaaaa 
      tgcgcaaca attgaccaa aaccttgga aagtttcaa 
      gccgggaaa cgtcccgaa aatcaacgt aaagctcat 
      ttggaaaga tgttggagc taatccgga actggtggt 
      cgcacactc gcgcatttt tggcgaagt caatattct 
      gaggtactt agctagggt cacttcgga ctacttgaa 
      ctacgagac ttgtaataa tgttcacga aatcgatgc 
      gttacttga tgtttgtta caatattgt aaagtctaa 
      gaccaagac tgaaagggc gagtttgtt gcaagtttt 
      gaacaagtc gttggggaa gagtcagtg gttgggtga 
      caggagtca aatcagtat tgccgactt agccgccta 
      ctcagccat caaacaggc gaccaatcc tagtgaagt 
      taatgttaa tttaggaaa gtggatgca aagatgtgt 
      agcttgtca ataaaacgc ttgtttaaa ttagaggat 
      cacagattt atttaaaat tttgttaca cttaattac 
      attaaactt aggcagtgt aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY