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Gene information
General information
TC012120 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 2585638..2598037
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012120
    iBeetle screen iB_01929
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012120 RA
      MAMADSANE LMLLERVFL RLGSADSDE QLQNLLSKF 
      LTPVLLKLA SPEESVRKK VMELLIHVN KRVKTRPQI 
      QLPVEDLIK QYQDPASTS FVTNFTIIY IKLGFPRLS 
      ADRQAELVP VILNALEQK PVAHLDSIL LLVVLVLGK 
      VKVPTEPEK VATLFGLNE KPQIAKHLL DMLLDMLLL 
      PYGALSPQT PENEQRSVS LPVPPCMSL HSFKRITLN 
      NPLKPEELE EIKLGIVRF LAHGVFKDE DILIHLVVA 
      AADTRFTVA NLADMELKK VVGSVDWGS LSVVMPLFT 
      MFLGSQSKN LKEDQIKSA ASTRIRLKL LTYLCRITG 
      KAFAFPASI QLIFDSLYG SNTNTRLKT LALNFTMNL 
      VRYADEGPL SKVAPVLLS GLQKLIKEG EETHQGQAY 
      VLIGALAQR FPKIVYHDI NLLEMFFKN LESANPDLK 
      LQIREGLLN LILAYKFDV CLEEADKDG RLNILFALI 
      KFRMSSEES MVRFVAVRT LATIFPPEH VPSKYLLLL 
      LTGDSKDDV SAEAFKALY GTSRKTEID LSKKNKIIT 
      PQFPEIVSY VHSEAESLV KDTAKRFSV GNHFLPFNL 
      RTYVELIIY LRLCLVRNL EVPLTRDIL NHPCEHTPV 
      IREYLQGLY DPNKSIETN PILQYTSLV KQLLLANPS 
      VEPLSCMVE IIGCVPQLS SVLFKDTQW VRDQLNSTK 
      EEVREYAST LYSLVLSPD SKEFESAIN FLLKQSESK 
      NLEAQHGAL LALGACVQF ALTYNSVSR DVHKKTVST 
      LVTFLKHQN PQLVGAACS SLGLIVQKS ALPLDDGKP 
      KEIGSPDAK RPANDIITK MDVVNRLMD VMNNTKLSA 
      KIREKAAKS LGLMCVGEK FPHTKEIIQ GLVNTSKDT 
      KDVEVHFTI GESLVMCCQ SVWSPEARN AWTTLPKDY 
      VPNTSEAMV DDNLDCLLD ELLKLAKMP HPNSKQASC 
      IWLLAILKG CGEREPITK KLQMLQNTF MDLLCENND 
      IVQDAASKG LCVVYDTYK SEELLTALV KQLTSGTRQ 
      VTQVTDDTK LFEEGQLGT APTGGNLTT YKELCSLAS 
      DLNKPDLIY QFMHLANHN AIWCSKKGA AFGFSSIAE 
      RCGEDLKAH LPLIIPKLY RYQFDPTPN IQASMHNIW 
      RVLVSEPQK TLDQYYDQI LADLLENLN SGQYRVRQS 
      CCLALQDFL KGAGNRSIH DCVNSMDEL WSKLFRVMD 
      DHHEATRLT ANKTARVLS KLCIRGCDV GQGKAGVKM 
      VEAILPPLL NNGITNPVA EIRLISLQT ISELVGSAG 
      SQLKPFLPK LIPALLQAT GELESTKLS YLSTMLGAQ 
      SQAQEAVDS ARASFAKSH FTTETVSKS LQYADASIL 
      EELVPKIVE LLKGSVGLG TRIACTHFV TLLVVQMGQ 
      DLQPYTGKL LAALVNGLT DRNAAIRKH YASAIGHLV 
      STAKESSLE KLFAKLQHW YFEREDDSI RSACAYTIQ 
      SIGIHNQEV LKSHSEVIL PLVFFAMHC EKTPETEST 
      LEVWTEIWS EHSPGTETG IRQNIEVIC DILKTALES 
      PSWTMKAQA ASAVSTVAS KLGTTMDAK HRNALINIL 
      LNGLSGRTW NGKDKLLKA LASICSNCR DSIKEDEGV 
      NVDLIIDAV IKESRKDEI GYKIKALGS LGDVLSALE 
      VDKFDEVYA IIQSILTDD NSVKEEDED ISSEEMVKK 
      RENSIKLKE TAYETLGKT WPAKSKLTQ DKYGEMFVE 
      HCVKCLPCI TRSVQVSVV SALYNYVDK LILLNEELA 
      ADDKKKLGR IVDKIIIAI KYALGISKH TRLRKESLN 
      VVFCMGNKL KQKNHAEEF QKLREMFDQ ALVDISGDN 
      QPEIKSRLT DIKNLLKTN
    CDS sequence
    • >TC012120 RA
      atggccatg gcggactct gcaaatgaa ttgatgctc 
      ctggagcgg gtgtttttg cgtttgggc tcagctgat 
      agtgacgag cagctccaa aacctcctt tctaagttc 
      ctgaccccc gttttacta aaactggca tcccctgag 
      gaaagcgtt cgcaagaaa gtgatggag ctgctaata 
      catgtaaat aaacgggtg aaaacgcgg cctcaaatc 
      cagctgcct gtggaagat ttgatcaaa cagtatcaa 
      gacccggca tctacctca tttgtcacg aattttaca 
      attatttat attaaactg gggttccca cgactgagt 
      gctgacaga caggccgaa cttgtccca gtcattttg 
      aatgcgttg gagcagaaa cctgttgct catttggac 
      agcattctt ttgttggtt gtactggtg ttgggtaaa 
      gttaaggtc ccgactgag cctgagaag gtggcgact 
      ttgtttggc ttgaatgag aagccgcaa attgccaaa 
      catttgctt gacatgctc ttggatatg cttctcttg 
      ccttatggg gcgctttca cctcaaacg cctgaaaat 
      gagcaaaga tcagtatcc ctccctgtg cccccttgc 
      atgagcctt cactccttt aaacgcata actttaaac 
      aaccccttg aaaccagaa gaactggaa gagatcaaa 
      ctgggaatc gtacgattt ctagcacat ggtgttttc 
      aaagacgag gatatttta attcacctc gtggtagca 
      gccgccgat acgcgtttc actgtagct aatttagcc 
      gacatggaa ttgaaaaaa gtcgtcggc tctgtagat 
      tggggctcc ctaagtgtc gttatgccc ctatttacc 
      atgttttta ggttcacaa tcgaaaaat ctaaaagag 
      gatcagatc aaatcagct gctagtacc aggataagg 
      cttaaactt ttgacttat ttgtgccga atcacagga 
      aaggctttc gcatttccc gcttccatt cagctgatt 
      tttgactcg ttgtacggc agtaacacc aacaccagg 
      ctcaagact ttggccctg aacttcacc atgaatctc 
      gtcagatac gctgacgag ggccccttg agcaaagtg 
      gcccccgtg ctgttgtca ggcctccaa aaactgatc 
      aaggaaggc gaagagacg caccaaggc caagcctat 
      gtcttgata ggggcccta gcacagaga ttcccgaaa 
      atcgtttac cacgatatc aaccttttg gagatgttt 
      ttcaaaaat ttggaatca gccaatccc gacttaaaa 
      ttgcaaatt cgtgaaggc ctccttaat ctaattctg 
      gcatacaag ttcgatgtt tgtttggag gaagccgat 
      aaagacggt cgcttgaat atcctcttc gctttgatc 
      aaatttcgg atgagttcc gaagagtca atggtgagg 
      tttgtggcg gtcagaact ctagctacg atttttcca 
      cctgaacac gtcccgtcc aagtatttg ttactatta 
      ttgacgggt gatagcaaa gatgacgtg tctgctgaa 
      gcttttaag gcgctttat ggaacgtca cgaaagaca 
      gaaatagat ttatcgaaa aagaataaa attatcaca 
      ccgcagttt ccggaaatt gtgagttat gtccacagc 
      gaggccgaa agtttagtc aaagacacc gcaaaaagg 
      ttttcggtt ggaaaccac tttctaccg ttcaaccta 
      cgaacatac gttgagttg attatttat ttgcgactt 
      tgcttggtc cgaaatttg gaagtccct ctaactcgt 
      gatattctg aaccacccg tgtgagcac acgccggtc 
      attcgagag taccttcaa ggtctttac gacccaaac 
      aagtcaatc gaaactaat ccaatcctt cagtacact 
      tcactagtc aagcaattg ctactagca aatccttct 
      gtagagccc ctaagttgc atggttgaa ataatcggt 
      tgcgtcccc caattaagt tcagtgttg ttcaaagac 
      acacaatgg gtccgagat cagttgaac tcgaccaag 
      gaagaagtc cgagaatac gcttccacg ctttactcc 
      cttgtactt agtcccgat tcaaaggag tttgaaagt 
      gccattaat tttttgtta aaacaatcc gaaagtaaa 
      aatctggag gcccaacat ggggccctt ttagcgctt 
      ggtgcttgt gtacagttc gcactgacg tataattca 
      gtcagtcgt gacgtgcac aaaaaaaca gtctcaaca 
      ctagttacg tttttgaag caccagaac ccgcaactg 
      gttggcgct gcttgtagt agtctcggt ttgatagta 
      cagaaatct gcattacct ctcgatgac ggtaagccc 
      aaagaaata ggcagtccc gacgccaaa cgcccagct 
      aacgatatc ataacgaaa atggacgtc gttaatcgc 
      ttaatggac gtgatgaac aacacgaaa ttgtcggcg 
      aaaattcgg gaaaaggcc gccaagtcc ctcggactg 
      atgtgtgtg ggggagaag ttcccgcac actaaggag 
      ataatccaa gggcttgtt aacacgtcg aaggacacc 
      aaagacgtg gaggtgcat tttacgatc ggggagtcg 
      ttggtgatg tgttgtcag agtgtgtgg tcgccggag 
      gcgcgaaat gcgtggacg acgcttccg aaggattat 
      gttccgaat acgagtgaa gctatggtt gatgataat 
      ttggattgt ttgttggat gagttgctg aagttggcg 
      aaaatgccg catccgaat tcgaagcaa gcgtcgtgt 
      atttggttg ttggcgatt ttgaaaggg tgtggggag 
      agggaaccg attacgaag aagttacaa atgttgcaa 
      aatacgttc atggatttg ctgtgcgaa aataacgat 
      attgtacaa gacgccgcc tctaagggt ttatgtgtc 
      gtctacgac acgtacaaa tcagaggaa ttactcaca 
      gcactggta aaacaattg acttcgggt actcgccaa 
      gtgacccaa gtcacagac gatacaaaa ttgtttgaa 
      gagggccag ttaggtaca gctccaact gggggtaac 
      ttaacaacg tacaaagaa ctgtgctca ttggcatcc 
      gatttaaac aaacccgac ttgatttac caatttatg 
      catcttgca aaccacaac gcaatctgg tgttcgaaa 
      aaaggcgca gctttcggc ttctcgtca atagccgaa 
      cgctgcggc gaagaccta aaagcacac ctaccttta 
      atcatccca aaattgtac agataccaa tttgaccca 
      acccccaat attcaagcg tccatgcac aacatttgg 
      agggtgttg gtttcagag ccgcagaaa actttggac 
      caatattac gaccaaatt ctagccgat cttttggaa 
      aatttaaat tcaggccag tatcgagtg cgccagtcg 
      tgttgtctc gccttgcag gatttccta aaaggggcg 
      ggaaaccgc tcgattcac gactgtgtc aactcgatg 
      gacgaactc tggagcaaa ctgttccgt gtcatggac 
      gaccaccac gaagccacg agactcaca gcaaataaa 
      accgccaga gttttgagc aaattgtgt atccgaggg 
      tgtgacgtg gggcagggc aaagccggg gttaaaatg 
      gtcgaggct attctgcct ccgctgctg aataacggg 
      atcacgaac ccggtcgct gaaattcgc ctgataagt 
      cttcagact atttcagaa ctcgtcggt tcggccggc 
      tcgcagctg aaaccgttc ttgccaaag ttaattccg 
      gcgcttttg caagccacg ggcgagttg gaatcgacg 
      aaactgtcg tatttgagc accatgttg ggggcgcaa 
      agccaggcc caggaggct gtggacagt gcgagggct 
      tcttttgcc aaatcgcat ttcactacg gaaacagtt 
      tcgaaaagt ctacagtac gccgatgcg tcgattttg 
      gaggagttg gttccgaaa attgttgaa ttgttgaag 
      ggttcggtt gggttgggg acgaggatt gcatgtacg 
      cattttgtg acacttttg gtggtgcag atgggacag 
      gatttgcaa ccctatact gggaagttg ttagcagca 
      cttgtaaat gggttaact gatcgaaat gccgcaata 
      aggaagcat tatgcaagt gctattggg catttggtt 
      tcaacggcc aaagaatca agtttggag aagttgttt 
      gccaagttg cagcattgg tactttgag cgtgaagac 
      gattcgatc cgttcggca tgcgcttac acaattcag 
      tcgatcgga atccacaac caggaagtg ctaaaatcg 
      cactcggag gtgattctg cccctggtg ttttttgca 
      atgcattgc gagaaaacg ccggagacg gaaagcact 
      cttgaggtg tggacggaa atttggtcg gaacatagt 
      ccgggcacg gaaaccgga atccggcaa aatatcgaa 
      gtcatctgc gatatcctg aaaacggcg ttagaatca 
      ccttcgtgg acgatgaag gcccaggcg gcgagtgcc 
      gtttcgacg gtggcaagc aaattaggt acgacgatg 
      gatgccaaa cataggaat gcccttata aatattcta 
      ttgaatggt ttatcagga cggacatgg aatgggaaa 
      gataaattg ttaaaagcg ctcgctagt atttgttca 
      aattgcagg gattcaatc aaggaggat gaaggtgtt 
      aacgtcgac ttgataata gacgctgtg attaaagag 
      agtcgtaaa gacgagatc gggtataaa ataaaagcg 
      ttggggtct ttaggggac gttttatcg gctctagaa 
      gtggacaag ttcgacgag gtctatgca ataattcag 
      tcgatcttg accgatgac aactcggtg aaggaagaa 
      gacgaagat atttcgtcg gaggaaatg gtgaagaaa 
      cgcgagaat agcattaaa ttgaaagaa acggcttac 
      gaaactttg ggtaaaaca tggcctgca aaaagcaaa 
      ctcacccaa gataaatac ggggaaatg ttcgtcgag 
      cattgcgtt aagtgctta ccgtgtatt acacgctcc 
      gtgcaagtc agtgtagtg tcagcactg tacaattac 
      gtcgataaa ctgatctta ttaaacgag gaattggcc 
      gcagacgat aagaaaaag ttgggcaga atcgtcgat 
      aaaatcata attgcgatt aaatacgct ttgggaata 
      tcgaaacac acgcggctc cgcaaggag tcgctcaac 
      gtcgtgttc tgcatgggt aacaaactc aagcagaaa 
      aaccacgcg gaggagttc caaaaactg cgtgaaatg 
      ttcgaccag gccttggtc gacatttcc ggcgataat 
      caaccggaa atcaagtcg agacttaca gatattaaa 
      aatttgttg aagaccaat taa
    mRNA sequence
    • >TC012120 RA
      atgaatgtt gtaattttg caacgtttt cgaattttt 
      gtgttaggg attttttgc tacatttgt ttgacagga 
      gctgccaaa taaataaat atggccatg gcggactct 
      gcaaatgaa ttgatgctc ctggagcgg gtgtttttg 
      cgtttgggc tcagctgat agtgacgag cagctccaa 
      aacctcctt tctaagttc ctgaccccc gttttacta 
      aaactggca tcccctgag gaaagcgtt cgcaagaaa 
      gtgatggag ctgctaata catgtaaat aaacgggtg 
      aaaacgcgg cctcaaatc cagctgcct gtggaagat 
      ttgatcaaa cagtatcaa gacccggca tctacctca 
      tttgtcacg aattttaca attatttat attaaactg 
      gggttccca cgactgagt gctgacaga caggccgaa 
      cttgtccca gtcattttg aatgcgttg gagcagaaa 
      cctgttgct catttggac agcattctt ttgttggtt 
      gtactggtg ttgggtaaa gttaaggtc ccgactgag 
      cctgagaag gtggcgact ttgtttggc ttgaatgag 
      aagccgcaa attgccaaa catttgctt gacatgctc 
      ttggatatg cttctcttg ccttatggg gcgctttca 
      cctcaaacg cctgaaaat gagcaaaga tcagtatcc 
      ctccctgtg cccccttgc atgagcctt cactccttt 
      aaacgcata actttaaac aaccccttg aaaccagaa 
      gaactggaa gagatcaaa ctgggaatc gtacgattt 
      ctagcacat ggtgttttc aaagacgag gatatttta 
      attcacctc gtggtagca gccgccgat acgcgtttc 
      actgtagct aatttagcc gacatggaa ttgaaaaaa 
      gtcgtcggc tctgtagat tggggctcc ctaagtgtc 
      gttatgccc ctatttacc atgttttta ggttcacaa 
      tcgaaaaat ctaaaagag gatcagatc aaatcagct 
      gctagtacc aggataagg cttaaactt ttgacttat 
      ttgtgccga atcacagga aaggctttc gcatttccc 
      gcttccatt cagctgatt tttgactcg ttgtacggc 
      agtaacacc aacaccagg ctcaagact ttggccctg 
      aacttcacc atgaatctc gtcagatac gctgacgag 
      ggccccttg agcaaagtg gcccccgtg ctgttgtca 
      ggcctccaa aaactgatc aaggaaggc gaagagacg 
      caccaaggc caagcctat gtcttgata ggggcccta 
      gcacagaga ttcccgaaa atcgtttac cacgatatc 
      aaccttttg gagatgttt ttcaaaaat ttggaatca 
      gccaatccc gacttaaaa ttgcaaatt cgtgaaggc 
      ctccttaat ctaattctg gcatacaag ttcgatgtt 
      tgtttggag gaagccgat aaagacggt cgcttgaat 
      atcctcttc gctttgatc aaatttcgg atgagttcc 
      gaagagtca atggtgagg tttgtggcg gtcagaact 
      ctagctacg atttttcca cctgaacac gtcccgtcc 
      aagtatttg ttactatta ttgacgggt gatagcaaa 
      gatgacgtg tctgctgaa gcttttaag gcgctttat 
      ggaacgtca cgaaagaca gaaatagat ttatcgaaa 
      aagaataaa attatcaca ccgcagttt ccggaaatt 
      gtgagttat gtccacagc gaggccgaa agtttagtc 
      aaagacacc gcaaaaagg ttttcggtt ggaaaccac 
      tttctaccg ttcaaccta cgaacatac gttgagttg 
      attatttat ttgcgactt tgcttggtc cgaaatttg 
      gaagtccct ctaactcgt gatattctg aaccacccg 
      tgtgagcac acgccggtc attcgagag taccttcaa 
      ggtctttac gacccaaac aagtcaatc gaaactaat 
      ccaatcctt cagtacact tcactagtc aagcaattg 
      ctactagca aatccttct gtagagccc ctaagttgc 
      atggttgaa ataatcggt tgcgtcccc caattaagt 
      tcagtgttg ttcaaagac acacaatgg gtccgagat 
      cagttgaac tcgaccaag gaagaagtc cgagaatac 
      gcttccacg ctttactcc cttgtactt agtcccgat 
      tcaaaggag tttgaaagt gccattaat tttttgtta 
      aaacaatcc gaaagtaaa aatctggag gcccaacat 
      ggggccctt ttagcgctt ggtgcttgt gtacagttc 
      gcactgacg tataattca gtcagtcgt gacgtgcac 
      aaaaaaaca gtctcaaca ctagttacg tttttgaag 
      caccagaac ccgcaactg gttggcgct gcttgtagt 
      agtctcggt ttgatagta cagaaatct gcattacct 
      ctcgatgac ggtaagccc aaagaaata ggcagtccc 
      gacgccaaa cgcccagct aacgatatc ataacgaaa 
      atggacgtc gttaatcgc ttaatggac gtgatgaac 
      aacacgaaa ttgtcggcg aaaattcgg gaaaaggcc 
      gccaagtcc ctcggactg atgtgtgtg ggggagaag 
      ttcccgcac actaaggag ataatccaa gggcttgtt 
      aacacgtcg aaggacacc aaagacgtg gaggtgcat 
      tttacgatc ggggagtcg ttggtgatg tgttgtcag 
      agtgtgtgg tcgccggag gcgcgaaat gcgtggacg 
      acgcttccg aaggattat gttccgaat acgagtgaa 
      gctatggtt gatgataat ttggattgt ttgttggat 
      gagttgctg aagttggcg aaaatgccg catccgaat 
      tcgaagcaa gcgtcgtgt atttggttg ttggcgatt 
      ttgaaaggg tgtggggag agggaaccg attacgaag 
      aagttacaa atgttgcaa aatacgttc atggatttg 
      ctgtgcgaa aataacgat attgtacaa gacgccgcc 
      tctaagggt ttatgtgtc gtctacgac acgtacaaa 
      tcagaggaa ttactcaca gcactggta aaacaattg 
      acttcgggt actcgccaa gtgacccaa gtcacagac 
      gatacaaaa ttgtttgaa gagggccag ttaggtaca 
      gctccaact gggggtaac ttaacaacg tacaaagaa 
      ctgtgctca ttggcatcc gatttaaac aaacccgac 
      ttgatttac caatttatg catcttgca aaccacaac 
      gcaatctgg tgttcgaaa aaaggcgca gctttcggc 
      ttctcgtca atagccgaa cgctgcggc gaagaccta 
      aaagcacac ctaccttta atcatccca aaattgtac 
      agataccaa tttgaccca acccccaat attcaagcg 
      tccatgcac aacatttgg agggtgttg gtttcagag 
      ccgcagaaa actttggac caatattac gaccaaatt 
      ctagccgat cttttggaa aatttaaat tcaggccag 
      tatcgagtg cgccagtcg tgttgtctc gccttgcag 
      gatttccta aaaggggcg ggaaaccgc tcgattcac 
      gactgtgtc aactcgatg gacgaactc tggagcaaa 
      ctgttccgt gtcatggac gaccaccac gaagccacg 
      agactcaca gcaaataaa accgccaga gttttgagc 
      aaattgtgt atccgaggg tgtgacgtg gggcagggc 
      aaagccggg gttaaaatg gtcgaggct attctgcct 
      ccgctgctg aataacggg atcacgaac ccggtcgct 
      gaaattcgc ctgataagt cttcagact atttcagaa 
      ctcgtcggt tcggccggc tcgcagctg aaaccgttc 
      ttgccaaag ttaattccg gcgcttttg caagccacg 
      ggcgagttg gaatcgacg aaactgtcg tatttgagc 
      accatgttg ggggcgcaa agccaggcc caggaggct 
      gtggacagt gcgagggct tcttttgcc aaatcgcat 
      ttcactacg gaaacagtt tcgaaaagt ctacagtac 
      gccgatgcg tcgattttg gaggagttg gttccgaaa 
      attgttgaa ttgttgaag ggttcggtt gggttgggg 
      acgaggatt gcatgtacg cattttgtg acacttttg 
      gtggtgcag atgggacag gatttgcaa ccctatact 
      gggaagttg ttagcagca cttgtaaat gggttaact 
      gatcgaaat gccgcaata aggaagcat tatgcaagt 
      gctattggg catttggtt tcaacggcc aaagaatca 
      agtttggag aagttgttt gccaagttg cagcattgg 
      tactttgag cgtgaagac gattcgatc cgttcggca 
      tgcgcttac acaattcag tcgatcgga atccacaac 
      caggaagtg ctaaaatcg cactcggag gtgattctg 
      cccctggtg ttttttgca atgcattgc gagaaaacg 
      ccggagacg gaaagcact cttgaggtg tggacggaa 
      atttggtcg gaacatagt ccgggcacg gaaaccgga 
      atccggcaa aatatcgaa gtcatctgc gatatcctg 
      aaaacggcg ttagaatca ccttcgtgg acgatgaag 
      gcccaggcg gcgagtgcc gtttcgacg gtggcaagc 
      aaattaggt acgacgatg gatgccaaa cataggaat 
      gcccttata aatattcta ttgaatggt ttatcagga 
      cggacatgg aatgggaaa gataaattg ttaaaagcg 
      ctcgctagt atttgttca aattgcagg gattcaatc 
      aaggaggat gaaggtgtt aacgtcgac ttgataata 
      gacgctgtg attaaagag agtcgtaaa gacgagatc 
      gggtataaa ataaaagcg ttggggtct ttaggggac 
      gttttatcg gctctagaa gtggacaag ttcgacgag 
      gtctatgca ataattcag tcgatcttg accgatgac 
      aactcggtg aaggaagaa gacgaagat atttcgtcg 
      gaggaaatg gtgaagaaa cgcgagaat agcattaaa 
      ttgaaagaa acggcttac gaaactttg ggtaaaaca 
      tggcctgca aaaagcaaa ctcacccaa gataaatac 
      ggggaaatg ttcgtcgag cattgcgtt aagtgctta 
      ccgtgtatt acacgctcc gtgcaagtc agtgtagtg 
      tcagcactg tacaattac gtcgataaa ctgatctta 
      ttaaacgag gaattggcc gcagacgat aagaaaaag 
      ttgggcaga atcgtcgat aaaatcata attgcgatt 
      aaatacgct ttgggaata tcgaaacac acgcggctc 
      cgcaaggag tcgctcaac gtcgtgttc tgcatgggt 
      aacaaactc aagcagaaa aaccacgcg gaggagttc 
      caaaaactg cgtgaaatg ttcgaccag gccttggtc 
      gacatttcc ggcgataat caaccggaa atcaagtcg 
      agacttaca gatattaaa aatttgttg aagaccaat 
      taattttta agtttgtaa agttgccaa taaacgaat 
      ttttcgacc