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Gene information
General information
TC034315
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012090
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid66: 3215870..3217627
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034315
    iBeetle screen iB_01928
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034315 RA
      MSHTVTVTR TTTTTTTSA IIINTGYLK 
      TWPGILKLL QMLLGIVVV GLVSFYYPD 
      YLHVSQAPK TFFLLMAVT FLVGTFLLL 
      LSCLISIST ASIISKTIY EVVYHGFAF 
      VLYLAASLT FIIEVNHWK SSYGRDYEP 
      YFAAAIIGL VLSALYLLS TVFALRSYR 
      GL
    CDS sequence
    • >TC034315 RA
      atgtcgcac acggtcacg gttacaagg accacaacc 
      accacaacg acctccgca atcatcata aatacggga 
      tatttgaaa acatggcct ggaattttg aaactattg 
      caaatgctc ctggggatc gtggtcgtg ggcctggtc 
      agcttctac taccccgac tacctgcac gtgagccag 
      gcccccaag accttcttc ctgctgatg gcggtcacg 
      ttcctggtc gggacattc ctgctgctg ctctcgtgc 
      ctgatctcc atttcgacg gcgtcgatc atttccaag 
      accatctat gaagtggtt taccacggt ttcgccttt 
      gtgctctat ctcgctgcc agtctgacg ttcatcatt 
      gaagtaaat cactggaaa agcagctac gggcgcgac 
      tacgagccg tattttgca gctgcgatt atcgggctt 
      gtgctctcg gcgttgtat ttgctgagc acggtgttt 
      gccctgagg agctacagg gggttgtaa
    mRNA sequence
    • >TC034315 RA
      gaccagaat tcagtttgt ttccagaca ttcgtgggc 
      tttcgttag ttcgatcgg tcggttaca cccacctta 
      ccataaaca aaactccaa caggactag cgaaaatgt 
      cgcacacgg tcacggtta caaggacca caaccacca 
      caacgacct ccgcaatca tcataaata cgggatatt 
      tgaaaacat ggcctggaa ttttgaaac tattgcaaa 
      tgctcctgg ggatcgtgg tcgtgggcc tggtcagct 
      tctactacc ccgactacc tgcacgtga gccaggccc 
      ccaagacct tcttcctgc tgatggcgg tcacgttcc 
      tggtcggga cattcctgc tgctgctct cgtgcctga 
      tctccattt cgacggcgt cgatcattt ccaagacca 
      tctatgaag tggtttacc acggtttcg cctttgtgc 
      tctatctcg ctgccagtc tgacgttca tcattgaag 
      taaatcact ggaaaagca gctacgggc gcgactacg 
      agccgtatt ttgcagctg cgattatcg ggcttgtgc 
      tctcggcgt tgtatttgc tgagcacgg tgtttgccc 
      tgaggagct acagggggt tgtaagttg ttgaagttg 
      attgttttt catggttcg ttgcaccac atctacgtt 
      agctttaaa tgtttatgt aatttattg ccagtattt 
      aggattatt ttttgagcc acttaaaac gcaaggcaa 
      agagcaata atttaatag tgtagtatc agtatttat 
      ttattgtgt gaggggtgt cttacattt ctaggacga 
      acccttgaa gtcacttgc tactaggat tagagacta 
      acgtttgta aacaatagt tgtaaggat attgtatac 
      agagctctc tactaagca tttcttgtc agatgtaac 
      aggagtagg aaatattca tctgacaag gttgtaagc 
      tgccattga tgtctatta atgtgccta aaattgtat 
      gccttagca cttttgtaa taggtttat tgtgaacgt 
      ttatacata ttttatgga gatatttag aattttata 
      agtgttgaa tgtactgga gttctattt aatttttgt 
      ataggatat ttgtgattg tacaactat ttaaaaata 
      aaattatat aaaggat