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Gene information
General information
TC012073 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6669723..6678500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012073
    iBeetle screen iB_01926

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012073 RA
      MSDMTPAVE QWSLYSAAQ NLPAVLNDP NRGKQSNFF 
      VKTWGDTFV EKTQIEKSP FLPEITYSH FDGYVRKIG 
      KRYKRHIRL SQSKLEANH VKTRTKADL INGEVSVES 
      IPEIFLRQH FPLNDPAVF AQVFHGNDR DQDYVLQEE 
      LSHYLDIVE VQIAKQVSQ KSGAFFHAM TSHDTIMEQ 
      MGVAHTEVQ TLRSKVQKV DKTLAKDSL KLVGLARAK 
      ANHGKLVDK LKLMATVLQ TQPTLQLLL SSSDYVAAL 
      ELISSTQEV LSKELAGVT SLRHLPSQL KEMSKLIDK 
      MLSTEFERY AAADLHRPL EAEGGVLEQ ERLVSLVAG 
      LLRQNNLQF LETYKQEAV TAAQTLLKQ LLIEQLADV 
      EDDMEMTGS GEVAPSMDA AHWLRVLTL ASEALGKLV 
      QRVKSVHSV IKETADTSA GLKSAPSLR DSESFLSLE 
      DHNRVEIKL KDLLISICD YCHERLASL VSTQSDKQT 
      ITAVQVTEL STIVDNFTD LCEKVCGRQ SAALRAAFK 
      IQAGNYVHK FHSQRKNKL TMLLDAERW KVAEVPQEI 
      QMLVDKLAL GNVIKSLPS SPTGENNEK KLQKPAPAL 
      KIGSQDYTT VGTVLILIQ LVCEYCVCA YNLQLLAPI 
      VGRNLTELL RTFNSRSCQ LVLGAGALK TAGLKTITT 
      TNLALTSRS LQMVLWMIP HIRAHFNAL TSDPLGGFD 
      AVERDIGHH IQQLETKVL SIMNSLLGD QLNEWDAKP 
      PVPSKAFRN VSRHLTKLH EAVSPVLPE EQVTDIYEI 
      IHKNFKSRL RDQLLKMNI QNNGGPQHG LVTTEIFFY 
      IDTMKTLKV LPDKYLSDK AMDDIWTR
    CDS sequence
    • >TC012073 RA
      atgagtgac atgacgcca gctgttgag cagtggtcc 
      ttatactcc gctgctcaa aacctcccg gccgtccta 
      aacgacccc aatcgcggc aaacagtcg aatttcttc 
      gtcaagaca tggggcgac acattcgtc gagaagacc 
      caaatcgag aaaagcccc ttcctgccc gaaataacc 
      tactcgcac ttcgatggc tacgttcgc aagatcggc 
      aaacggtac aagcggcac attcggctc tcccagagc 
      aagctcgag gcgaatcat gtgaaaact cgaacgaag 
      gcggacctc atcaacggc gaggttagc gtcgaatca 
      atccccgag attttcctg cggcagcat ttccctctg 
      aacgatccc gcagtgttc gcgcaagtt ttccacgga 
      aacgaccgg gaccaggac tacgtcctg caggaggag 
      ttgagtcat tacttggac atcgtcgag gtgcaaatt 
      gccaagcag gtgtcgcaa aagtcgggg gcttttttc 
      cacgccatg acctcgcac gacaccatc atggagcag 
      atgggagtt gcccacacg gaggtgcaa accttgcgc 
      tcgaaagtg caaaaggtg gacaaaacc ctcgcgaag 
      gactccctc aagctcgta ggcctggcc agggccaag 
      gccaaccac ggcaagctc gtcgacaag ctcaagtta 
      atggcaact gttttacaa acacagccc actctccag 
      ctcctcctg agctcgtcg gattacgtc gcagccctc 
      gaacttatt tcgagcacc caggaggtg ctgtcgaag 
      gagctggct ggggttact agtctgcgg cacttgccc 
      tcgcagctt aaagaaatg tcgaagttg attgataag 
      atgttgagt acggagttt gagagatac gctgccgcc 
      gatttgcac cggcctttg gaggcggaa ggtggggtt 
      ttggagcag gagaggttg gttagtttg gtcgcgggg 
      ctgttgcga cagaacaat ttgcaattt ttggagacg 
      tataagcag gaggctgtc actgcggct cagacttta 
      ctcaaacag ttgctgatt gaacagttg gcggatgtt 
      gaggatgat atggagatg acggggtcg ggggaggtg 
      gcccccagt atggacgcc gcgcattgg ttgagggtg 
      ctgacgctg gcaagtgaa gcgctgggc aagctggtc 
      cagagggtg aaatccgtt cacagcgtc atcaaagaa 
      acggcagat acgagcgcc ggcctcaaa tcagcgccc 
      tctctccgc gactcggaa agtttccta agtctcgaa 
      gaccacaac cgcgtggaa atcaaacta aaagactta 
      ttaatctca atctgtgat tattgccat gaacggtta 
      gcttcatta gtcagcact cagtccgac aagcagacc 
      ataacagca gtccaggtc actgaatta tcgacaatc 
      gtcgataac ttcaccgac ctgtgcgag aaggtttgt 
      gggcggcaa agtgccgct ttgagggcg gcattcaaa 
      atccaggcc gggaattac gtccacaaa ttccacagc 
      cagcgcaaa aacaaactc acgatgctt ctggacgcg 
      gaacggtgg aaagtcgct gaagtgcct caagaaatc 
      caaatgcta gtcgacaag ttagcttta gggaatgtg 
      atcaagtcg ttgccgagt tcgccaact ggggaaaac 
      aacgaaaag aaattacag aaacctgca ccggccctg 
      aaaatcggc tcgcaggat tacacgacg gtggggacc 
      gtgctgatc ctcatacag ctagtttgt gaatattgt 
      gtttgtgcg tacaatttg caattgctg gcccccata 
      gtcgggcgc aatttgacc gagttgttg cgaacgttc 
      aattcgcgg agttgtcag ctggtgctg ggggcaggt 
      gcgttgaag acagccggc ctgaagacg ataacgacg 
      acgaatttg gcgctgacg tccaggtcg ttgcagatg 
      gttttgtgg atgatcccg cacattcgg gcgcatttt 
      aatgcgctg accagcgac ccgctcggg gggtttgac 
      gcggtggag agagatatc ggacatcat attcaacag 
      ctggagacc aaagttttg tcgattatg aatagtttg 
      ttgggggat cagttgaat gaatgggat gcgaagccg 
      cccgtgccg tcgaaagcg ttcaggaat gtgtcgagg 
      catctgacg aagttgcac gaagcggtc agcccggtc 
      ttgcccgag gaacaggtc accgacatt tacgaaata 
      atccacaaa aacttcaag tcgcgcctt cgagaccag 
      ctcctaaaa atgaacatc caaaacaac ggcggcccc 
      cagcacggc ttagtcaca accgaaata tttttttac 
      atagacaca atgaagact ttaaaagtt ttgccagat 
      aagtatttg tcagataag gccatggat gatatttgg 
      acgaggtaa
    mRNA sequence
    • >TC012073 RA
      taaacctga cagttgatt ttttcatga catgagttg 
      tcaaagtag tggttttat cacctgata cagatgtgt 
      ctcaaataa ttgtgtccc ccaaaatga agaaataca 
      aaacggggt agtcccccg ggtcttatg agtgacatg 
      acgccagct gttgagcag tggtcctta tactccgct 
      gctcaaaac ctcccggcc gtcctaaac gaccccaat 
      cgcggcaaa cagtcgaat ttcttcgtc aagacatgg 
      ggcgacaca ttcgtcgag aagacccaa atcgagaaa 
      agccccttc ctgcccgaa ataacctac tcgcacttc 
      gatggctac gttcgcaag atcggcaaa cggtacaag 
      cggcacatt cggctctcc cagagcaag ctcgaggcg 
      aatcatgtg aaaactcga acgaaggcg gacctcatc 
      aacggcgag gttagcgtc gaatcaatc cccgagatt 
      ttcctgcgg cagcatttc cctctgaac gatcccgca 
      gtgttcgcg caagttttc cacggaaac gaccgggac 
      caggactac gtcctgcag gaggagttg agtcattac 
      ttggacatc gtcgaggtg caaattgcc aagcaggtg 
      tcgcaaaag tcgggggct tttttccac gccatgacc 
      tcgcacgac accatcatg gagcagatg ggagttgcc 
      cacacggag gtgcaaacc ttgcgctcg aaagtgcaa 
      aaggtggac aaaaccctc gcgaaggac tccctcaag 
      ctcgtaggc ctggccagg gccaaggcc aaccacggc 
      aagctcgtc gacaagctc aagttaatg gcaactgtt 
      ttacaaaca cagcccact ctccagctc ctcctgagc 
      tcgtcggat tacgtcgca gccctcgaa cttatttcg 
      agcacccag gaggtgctg tcgaaggag ctggctggg 
      gttactagt ctgcggcac ttgccctcg cagcttaaa 
      gaaatgtcg aagttgatt gataagatg ttgagtacg 
      gagtttgag agatacgct gccgccgat ttgcaccgg 
      cctttggag gcggaaggt ggggttttg gagcaggag 
      aggttggtt agtttggtc gcggggctg ttgcgacag 
      aacaatttg caatttttg gagacgtat aagcaggag 
      gctgtcact gcggctcag actttactc aaacagttg 
      ctgattgaa cagttggcg gatgttgag gatgatatg 
      gagatgacg gggtcgggg gaggtggcc cccagtatg 
      gacgccgcg cattggttg agggtgctg acgctggca 
      agtgaagcg ctgggcaag ctggtccag agggtgaaa 
      tccgttcac agcgtcatc aaagaaacg gcagatacg 
      agcgccggc ctcaaatca gcgccctct ctccgcgac 
      tcggaaagt ttcctaagt ctcgaagac cacaaccgc 
      gtggaaatc aaactaaaa gacttatta atctcaatc 
      tgtgattat tgccatgaa cggttagct tcattagtc 
      agcactcag tccgacaag cagaccata acagcagtc 
      caggtcact gaattatcg acaatcgtc gataacttc 
      accgacctg tgcgagaag gtttgtggg cggcaaagt 
      gccgctttg agggcggca ttcaaaatc caggccggg 
      aattacgtc cacaaattc cacagccag cgcaaaaac 
      aaactcacg atgcttctg gacgcggaa cggtggaaa 
      gtcgctgaa gtgcctcaa gaaatccaa atgctagtc 
      gacaagtta gctttaggg aatgtgatc aagtcgttg 
      ccgagttcg ccaactggg gaaaacaac gaaaagaaa 
      ttacagaaa cctgcaccg gccctgaaa atcggctcg 
      caggattac acgacggtg gggaccgtg ctgatcctc 
      atacagcta gtttgtgaa tattgtgtt tgtgcgtac 
      aatttgcaa ttgctggcc cccatagtc gggcgcaat 
      ttgaccgag ttgttgcga acgttcaat tcgcggagt 
      tgtcagctg gtgctgggg gcaggtgcg ttgaagaca 
      gccggcctg aagacgata acgacgacg aatttggcg 
      ctgacgtcc aggtcgttg cagatggtt ttgtggatg 
      atcccgcac attcgggcg cattttaat gcgctgacc 
      agcgacccg ctcgggggg tttgacgcg gtggagaga 
      gatatcgga catcatatt caacagctg gagaccaaa 
      gttttgtcg attatgaat agtttgttg ggggatcag 
      ttgaatgaa tgggatgcg aagccgccc gtgccgtcg 
      aaagcgttc aggaatgtg tcgaggcat ctgacgaag 
      ttgcacgaa gcggtcagc ccggtcttg cccgaggaa 
      caggtcacc gacatttac gaaataatc cacaaaaac 
      ttcaagtcg cgccttcga gaccagctc ctaaaaatg 
      aacatccaa aacaacggc ggcccccag cacggctta 
      gtcacaacc gaaatattt ttttacata gacacaatg 
      aagacttta aaagttttg ccagataag tatttgtca 
      gataaggcc atggatgat atttggacg aggtaataa 
      aaattccaa acggtgttt tatttatta agcttgaaa 
      gtatgtatg ctgcgaatg cccgaattt tgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY