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Gene information
General information
TC012073 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6669723..6678500
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012073
    iBeetle screen iB_01926

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012073 RA
      MSDMTPAVE QWSLYSAAQ NLPAVLNDP NRGKQSNFF 
VKTWGDTFV EKTQIEKSP FLPEITYSH FDGYVRKIG 
KRYKRHIRL SQSKLEANH VKTRTKADL INGEVSVES 
IPEIFLRQH FPLNDPAVF AQVFHGNDR DQDYVLQEE 
LSHYLDIVE VQIAKQVSQ KSGAFFHAM TSHDTIMEQ 
MGVAHTEVQ TLRSKVQKV DKTLAKDSL KLVGLARAK 
ANHGKLVDK LKLMATVLQ TQPTLQLLL SSSDYVAAL 
ELISSTQEV LSKELAGVT SLRHLPSQL KEMSKLIDK 
MLSTEFERY AAADLHRPL EAEGGVLEQ ERLVSLVAG 
LLRQNNLQF LETYKQEAV TAAQTLLKQ LLIEQLADV 
EDDMEMTGS GEVAPSMDA AHWLRVLTL ASEALGKLV 
QRVKSVHSV IKETADTSA GLKSAPSLR DSESFLSLE 
DHNRVEIKL KDLLISICD YCHERLASL VSTQSDKQT 
ITAVQVTEL STIVDNFTD LCEKVCGRQ SAALRAAFK 
IQAGNYVHK FHSQRKNKL TMLLDAERW KVAEVPQEI 
QMLVDKLAL GNVIKSLPS SPTGENNEK KLQKPAPAL 
KIGSQDYTT VGTVLILIQ LVCEYCVCA YNLQLLAPI 
VGRNLTELL RTFNSRSCQ LVLGAGALK TAGLKTITT 
TNLALTSRS LQMVLWMIP HIRAHFNAL TSDPLGGFD 
AVERDIGHH IQQLETKVL SIMNSLLGD QLNEWDAKP 
PVPSKAFRN VSRHLTKLH EAVSPVLPE EQVTDIYEI 
IHKNFKSRL RDQLLKMNI QNNGGPQHG LVTTEIFFY 
IDTMKTLKV LPDKYLSDK AMDDIWTR
    CDS sequence
    • >TC012073 RA
      atgagtgac atgacgcca gctgttgag cagtggtcc 
ttatactcc gctgctcaa aacctcccg gccgtccta 
aacgacccc aatcgcggc aaacagtcg aatttcttc 
gtcaagaca tggggcgac acattcgtc gagaagacc 
caaatcgag aaaagcccc ttcctgccc gaaataacc 
tactcgcac ttcgatggc tacgttcgc aagatcggc 
aaacggtac aagcggcac attcggctc tcccagagc 
aagctcgag gcgaatcat gtgaaaact cgaacgaag 
gcggacctc atcaacggc gaggttagc gtcgaatca 
atccccgag attttcctg cggcagcat ttccctctg 
aacgatccc gcagtgttc gcgcaagtt ttccacgga 
aacgaccgg gaccaggac tacgtcctg caggaggag 
ttgagtcat tacttggac atcgtcgag gtgcaaatt 
gccaagcag gtgtcgcaa aagtcgggg gcttttttc 
cacgccatg acctcgcac gacaccatc atggagcag 
atgggagtt gcccacacg gaggtgcaa accttgcgc 
tcgaaagtg caaaaggtg gacaaaacc ctcgcgaag 
gactccctc aagctcgta ggcctggcc agggccaag 
gccaaccac ggcaagctc gtcgacaag ctcaagtta 
atggcaact gttttacaa acacagccc actctccag 
ctcctcctg agctcgtcg gattacgtc gcagccctc 
gaacttatt tcgagcacc caggaggtg ctgtcgaag 
gagctggct ggggttact agtctgcgg cacttgccc 
tcgcagctt aaagaaatg tcgaagttg attgataag 
atgttgagt acggagttt gagagatac gctgccgcc 
gatttgcac cggcctttg gaggcggaa ggtggggtt 
ttggagcag gagaggttg gttagtttg gtcgcgggg 
ctgttgcga cagaacaat ttgcaattt ttggagacg 
tataagcag gaggctgtc actgcggct cagacttta 
ctcaaacag ttgctgatt gaacagttg gcggatgtt 
gaggatgat atggagatg acggggtcg ggggaggtg 
gcccccagt atggacgcc gcgcattgg ttgagggtg 
ctgacgctg gcaagtgaa gcgctgggc aagctggtc 
cagagggtg aaatccgtt cacagcgtc atcaaagaa 
acggcagat acgagcgcc ggcctcaaa tcagcgccc 
tctctccgc gactcggaa agtttccta agtctcgaa 
gaccacaac cgcgtggaa atcaaacta aaagactta 
ttaatctca atctgtgat tattgccat gaacggtta 
gcttcatta gtcagcact cagtccgac aagcagacc 
ataacagca gtccaggtc actgaatta tcgacaatc 
gtcgataac ttcaccgac ctgtgcgag aaggtttgt 
gggcggcaa agtgccgct ttgagggcg gcattcaaa 
atccaggcc gggaattac gtccacaaa ttccacagc 
cagcgcaaa aacaaactc acgatgctt ctggacgcg 
gaacggtgg aaagtcgct gaagtgcct caagaaatc 
caaatgcta gtcgacaag ttagcttta gggaatgtg 
atcaagtcg ttgccgagt tcgccaact ggggaaaac 
aacgaaaag aaattacag aaacctgca ccggccctg 
aaaatcggc tcgcaggat tacacgacg gtggggacc 
gtgctgatc ctcatacag ctagtttgt gaatattgt 
gtttgtgcg tacaatttg caattgctg gcccccata 
gtcgggcgc aatttgacc gagttgttg cgaacgttc 
aattcgcgg agttgtcag ctggtgctg ggggcaggt 
gcgttgaag acagccggc ctgaagacg ataacgacg 
acgaatttg gcgctgacg tccaggtcg ttgcagatg 
gttttgtgg atgatcccg cacattcgg gcgcatttt 
aatgcgctg accagcgac ccgctcggg gggtttgac 
gcggtggag agagatatc ggacatcat attcaacag 
ctggagacc aaagttttg tcgattatg aatagtttg 
ttgggggat cagttgaat gaatgggat gcgaagccg 
cccgtgccg tcgaaagcg ttcaggaat gtgtcgagg 
catctgacg aagttgcac gaagcggtc agcccggtc 
ttgcccgag gaacaggtc accgacatt tacgaaata 
atccacaaa aacttcaag tcgcgcctt cgagaccag 
ctcctaaaa atgaacatc caaaacaac ggcggcccc 
cagcacggc ttagtcaca accgaaata tttttttac 
atagacaca atgaagact ttaaaagtt ttgccagat 
aagtatttg tcagataag gccatggat gatatttgg 
acgaggtaa
    mRNA sequence
    • >TC012073 RA
      taaacctga cagttgatt ttttcatga catgagttg 
tcaaagtag tggttttat cacctgata cagatgtgt 
ctcaaataa ttgtgtccc ccaaaatga agaaataca 
aaacggggt agtcccccg ggtcttatg agtgacatg 
acgccagct gttgagcag tggtcctta tactccgct 
gctcaaaac ctcccggcc gtcctaaac gaccccaat 
cgcggcaaa cagtcgaat ttcttcgtc aagacatgg 
ggcgacaca ttcgtcgag aagacccaa atcgagaaa 
agccccttc ctgcccgaa ataacctac tcgcacttc 
gatggctac gttcgcaag atcggcaaa cggtacaag 
cggcacatt cggctctcc cagagcaag ctcgaggcg 
aatcatgtg aaaactcga acgaaggcg gacctcatc 
aacggcgag gttagcgtc gaatcaatc cccgagatt 
ttcctgcgg cagcatttc cctctgaac gatcccgca 
gtgttcgcg caagttttc cacggaaac gaccgggac 
caggactac gtcctgcag gaggagttg agtcattac 
ttggacatc gtcgaggtg caaattgcc aagcaggtg 
tcgcaaaag tcgggggct tttttccac gccatgacc 
tcgcacgac accatcatg gagcagatg ggagttgcc 
cacacggag gtgcaaacc ttgcgctcg aaagtgcaa 
aaggtggac aaaaccctc gcgaaggac tccctcaag 
ctcgtaggc ctggccagg gccaaggcc aaccacggc 
aagctcgtc gacaagctc aagttaatg gcaactgtt 
ttacaaaca cagcccact ctccagctc ctcctgagc 
tcgtcggat tacgtcgca gccctcgaa cttatttcg 
agcacccag gaggtgctg tcgaaggag ctggctggg 
gttactagt ctgcggcac ttgccctcg cagcttaaa 
gaaatgtcg aagttgatt gataagatg ttgagtacg 
gagtttgag agatacgct gccgccgat ttgcaccgg 
cctttggag gcggaaggt ggggttttg gagcaggag 
aggttggtt agtttggtc gcggggctg ttgcgacag 
aacaatttg caatttttg gagacgtat aagcaggag 
gctgtcact gcggctcag actttactc aaacagttg 
ctgattgaa cagttggcg gatgttgag gatgatatg 
gagatgacg gggtcgggg gaggtggcc cccagtatg 
gacgccgcg cattggttg agggtgctg acgctggca 
agtgaagcg ctgggcaag ctggtccag agggtgaaa 
tccgttcac agcgtcatc aaagaaacg gcagatacg 
agcgccggc ctcaaatca gcgccctct ctccgcgac 
tcggaaagt ttcctaagt ctcgaagac cacaaccgc 
gtggaaatc aaactaaaa gacttatta atctcaatc 
tgtgattat tgccatgaa cggttagct tcattagtc 
agcactcag tccgacaag cagaccata acagcagtc 
caggtcact gaattatcg acaatcgtc gataacttc 
accgacctg tgcgagaag gtttgtggg cggcaaagt 
gccgctttg agggcggca ttcaaaatc caggccggg 
aattacgtc cacaaattc cacagccag cgcaaaaac 
aaactcacg atgcttctg gacgcggaa cggtggaaa 
gtcgctgaa gtgcctcaa gaaatccaa atgctagtc 
gacaagtta gctttaggg aatgtgatc aagtcgttg 
ccgagttcg ccaactggg gaaaacaac gaaaagaaa 
ttacagaaa cctgcaccg gccctgaaa atcggctcg 
caggattac acgacggtg gggaccgtg ctgatcctc 
atacagcta gtttgtgaa tattgtgtt tgtgcgtac 
aatttgcaa ttgctggcc cccatagtc gggcgcaat 
ttgaccgag ttgttgcga acgttcaat tcgcggagt 
tgtcagctg gtgctgggg gcaggtgcg ttgaagaca 
gccggcctg aagacgata acgacgacg aatttggcg 
ctgacgtcc aggtcgttg cagatggtt ttgtggatg 
atcccgcac attcgggcg cattttaat gcgctgacc 
agcgacccg ctcgggggg tttgacgcg gtggagaga 
gatatcgga catcatatt caacagctg gagaccaaa 
gttttgtcg attatgaat agtttgttg ggggatcag 
ttgaatgaa tgggatgcg aagccgccc gtgccgtcg 
aaagcgttc aggaatgtg tcgaggcat ctgacgaag 
ttgcacgaa gcggtcagc ccggtcttg cccgaggaa 
caggtcacc gacatttac gaaataatc cacaaaaac 
ttcaagtcg cgccttcga gaccagctc ctaaaaatg 
aacatccaa aacaacggc ggcccccag cacggctta 
gtcacaacc gaaatattt ttttacata gacacaatg 
aagacttta aaagttttg ccagataag tatttgtca 
gataaggcc atggatgat atttggacg aggtaataa 
aaattccaa acggtgttt tatttatta agcttgaaa 
gtatgtatg ctgcgaatg cccgaattt tgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY