Status
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Gene information
General information
TC031235
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012069,TC012070
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6765853..6768839
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031235
    iBeetle screen iB_01924

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    elytra partially colour lighter - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031235 RA
      MSEEVIFTL FYLILSFCI IYPPVEFVS AGFTIPALF 
      SRVLGSEDE NFVSYHIKR TIVTLGVYS LLPLGYITA 
      LFASEYFQD VSSLIIDGS LFWKAFFTT SLALPILAL 
      YQIRNWIID DFKHHPIAV NLAKFCNNN NRDWKSVAG 
      DINTEFRRV DKICVQTSS VIKVIATEN WILKVKPFT 
      ISLAHQSDA SLVVQKADT HRISLQTTN ETQYLNIEV 
      KSARVGVET FIIRINASD FKDLEDRIA RDITILPNV 
      KFHKSVTEQ FVDVFKETI KNNARYETR EELDQCIGC 
      MQTRPSVKL QKLCGDNAG GNEGCTTCY CKPMWCVDC 
      MAKWFASRQ ESDQQSTWL SSKCTCPMC RAKFCILDV 
      SLLESIDVE E
    CDS sequence
    • >TC031235 RA
      atgtccgaa gaggtgatt ttcacctta ttttaccta 
      attttaagc ttctgtatt atctacccc cctgtcgag 
      ttcgtgtct gccggcttc accatccct gcgctcttc 
      agccgagtg ttgggctcc gaggacgaa aacttcgta 
      agttaccac ataaagcgc acaatcgta acattgggg 
      gtgtattca ctgttgcct ttggggtac atcaccgcc 
      ctctttgcc tcggagtat ttccaagac gttagttct 
      ttgataatc gacggttct ctcttttgg aaagcgttt 
      ttcacgacc agtctggcg ttgccaatt ttggcgctc 
      tatcagatt aggaactgg ataatcgac gattttaaa 
      caccacccg atcgcggtc aatttggcc aagttttgc 
      aacaataat aacagggat tggaaaagt gtcgccggt 
      gacatcaac accgaattt agacgcgtt gataaaatc 
      tgcgtccaa acgagctcg gtgattaaa gtgatagcc 
      accgaaaat tggattttg aaagtgaaa ccctttacg 
      atttcactg gcgcatcaa agcgacgcc agtttagtg 
      gtccagaaa gctgacacg caccggatt tcgctccaa 
      acgaccaac gaaacgcaa tacttaaac atcgaagtt 
      aagtcggcc cgagtcggt gtcgagacg tttattatc 
      cgcattaat gcctcggat ttcaaggac ttggaagac 
      agaatagcg agagatatc accattttg cccaacgtc 
      aagttccat aaatctgtg accgagcaa ttcgttgac 
      gtcttcaag gagactatt aaaaataac gcgcgctac 
      gagactaga gaggagctg gaccaatgt attgggtgc 
      atgcagacg cgccccagc gtcaagttg cagaagctg 
      tgtggggat aacgcgggc gggaatgag ggctgtacg 
      acttgctat tgcaagccg atgtggtgt gttgattgc 
      atggcgaaa tggttcgct tcgagacag gagagcgac 
      cagcagagt acgtggctg tcgtccaag tgcacgtgt 
      ccgatgtgc agggcgaag ttttgcatt ttagatgtg 
      tcgctactt gagagtatc gacgttgag gaataa
    mRNA sequence
    • >TC031235 RA
      tttatttac gttattgaa taaataatt gttttattt 
      agtccgttc attatgtcc gaagaggtg attttcacc 
      ttattttac ctaatttta agcttctgt attatctac 
      ccccctgtc gagttcgtg tctgccggc ttcaccatc 
      cctgcgctc ttcagccga gtgttgggc tccgaggac 
      gaaaacttc gtaagttac cacataaag cgcacaatc 
      gtaacattg ggggtgtat tcactgttg cctttgggg 
      tacatcacc gccctcttt gcctcggag tatttccaa 
      gacgttagt tctttgata atcgacggt tctctcttt 
      tggaaagcg tttttcacg accagtctg gcgttgcca 
      attttggcg ctctatcag attaggaac tggataatc 
      gacgatttt aaacaccac ccgatcgcg gtcaatttg 
      gccaagttt tgcaacaat aataacagg gattggaaa 
      agtgtcgcc ggtgacatc aacaccgaa tttagacgc 
      gttgataaa atctgcgtc caaacgagc tcggtgatt 
      aaagtgata gccaccgaa aattggatt ttgaaagtg 
      aaacccttt acgatttca ctggcgcat caaagcgac 
      gccagttta gtggtccag aaagctgac acgcaccgg 
      atttcgctc caaacgacc aacgaaacg caatactta 
      aacatcgaa gttaagtcg gcccgagtc ggtgtcgag 
      acgtttatt atccgcatt aatgcctcg gatttcaag 
      gacttggaa gacagaata gcgagagat atcaccatt 
      ttgcccaac gtcaagttc cataaatct gtgaccgag 
      caattcgtt gacgtcttc aaggagact attaaaaat 
      aacgcgcgc tacgagact agagaggag ctggaccaa 
      tgtattggg tgcatgcag acgcgcccc agcgtcaag 
      ttgcagaag ctgtgtggg gataacgcg ggcgggaat 
      gagggctgt acgacttgc tattgcaag ccgatgtgg 
      tgtgttgat tgcatggcg aaatggttc gcttcgaga 
      caggagagc gaccagcag agtacgtgg ctgtcgtcc 
      aagtgcacg tgtccgatg tgcagggcg aagttttgc 
      attttagat gtgtcgcta cttgagagt atcgacgtt 
      gaggaataa attgttgct taattttaa ctgtattat 
      ttcttgata aggttttat ctggagttg cctgaacga 
      gattgctaa tttggaaaa taaaaattt atttttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY