Status
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Gene information
General information
TC034323
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012064,TC012062,TC012063
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6824687..6842247
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034323
    iBeetle screen iB_01923

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034323 RA
      MKLTLSLLV FVLISTVNS AKILGVFPV PGRSHYILA 
      SSLMRALAE KGHDVTVIS CFGEKIPPK NGTYRDIVA 
      TGVFDEMQK EMNSKNMSM FEIGGASPL LNAFFMAVL 
      LPEMTEKAL KHENVQKFI NSDEKFDVV IVEQFANDA 
      QKALSTHFG APLITFSTL GANYWVNPL VGNPSPPSY 
      IPDLMSDYS VPMTFVERL ENSLVYVMN ELLYNFYIF 
      PKQNELMKK YIPRAPAHI NDVLYNSSI ILMNAHPSI 
      NQPVPYVPN MVDIGGFHV KPPKKLPQD LQEFLDGAK 
      DGVIYFSMG SNLKSADLP NDKRDAILK TFAKLKQKI 
      LWKWEDENL PGKPPNVKT AKWLPQQDI LAHPNVKLF 
      ITHGGLLST TETIYHGVP ILAVPIFGD QKLNARSAV 
      KGGYGVHLP YEELNEETL TNSINEVLS NKKYKENVQ 
      KRSKLFHDR LVSPIDTLT YWVEYVVRH RGAPHLRVA 
      ALDLPCYGV NSAKILGVM PVTGRSHYI LVSTLMRAL 
      AEKGHDVTV ISCWGEKNP PKNGTYRDI VVTGAFEEM 
      QKLMSGADM NLFDMDSQN PLVGAIFMA TMFPAMTEI 
      TLKHENVQK LINSGEKFD VVIVEQFAN DAQKGLSTH 
      FGAPLVSLS GVGANYWAN ALVGNPSPP SYIPDIMLD 
      YSVPMAFCE RVVNSFVYV FNELLHKFY IFRKHNEIM 
      KKYIPNAPA HISDVLYNN SIILMNSHP SINQPVPYV 
      PSMVDIGGF HVKPPKKLP QDLQEFLDG AKDGVIYFS 
      MGSNLKSAE LPNDKRDAI LKTFAKLKQ KVLWKWEEE 
      DLPGKSPNV KTAKWLPQQ DILVPFDLA PKMLVKMKT 
      TFLLLVLVL ISCYEANSA KILAVLPVA GRSHYILAS 
      TLMRALAER GHDVTVISC FGEKNPPKN GTYRDIVVT 
      GAMEELEKQ MSSDDVNMF EMEGINLFI NALFMAFML 
      PTMNEATLK HENVQKFIN SGEKFDVVI VEQFGNDAA 
      KALSTHFGA PLISLSAVG ASYWTNPLV GNPSPPSYI 
      PDLMSDYSV PMTFCERLV NSLVINQPV PHVPNMIEI 
      GGFHIKPPK KLPQDLQEF LDGAKDGII YFSMGSNLK 
      SADLPNDKR DAILKTFAK LKQKILWKW EEDDLPGKP 
      PNVKTAKWL PQQELLVLI SYYGVKSAK ILAVLPVAG 
      KSHYILAST LMRALAERG HDVTVISCW GEKNPPKNG 
      TYRDIVMTG AMEELQRHL SSKDSNMFT RKGVNVFVD 
      VFFATMIIY RFNELTMKH ENVQKFIRS GEKFDVVIV 
      EQFANDALK ALSTHFGAP LISLSAMGA SYWTNPLVG 
      NPSPPSYIP HFSAKILGV FPVPGRSHY ILGSSLMKA 
      LAEKGHDVT VISCFEEQN PPKNGTYRD IVITGAVDE 
      MTKQMAKMN MFDRESTNP FAGAVFMSF RLPAMTETT 
      LKHENVQKL INSNEKFDA VIVEFASEA FATRASHWI 
      NPLVGNPSP PSYIPDIML DFSVLMTLY ERLVNSLVY 
      VFNELLLNF VVYPKHNEL MKKYIPNAP SHISEVLYN 
      HSIVLVNSH PSVNRPVPY VPSMVDIGG FHIKPPKKL 
      PQDLQEFLD SAKHGVIYF SLGSNLKSA QLPLEKRNA 
      LLQTFAKLK QKILWKWED EDLPGKPPN VKVAKWLPQ 
      QDILAHPNV KLFITHGGQ SSTTETIYH GVPILGIPI 
      FGDQKINAK SVARDGCGL YVAYSEITE EKLTESINE 
      ILHNQKYKE NVQRRSKLF HDRIVSPLD TAIYWVEYV 
      IRHRGAPHL RVAALDLP
    CDS sequence
    • >TC034323 RA
      atgaagctt acattatct ctactagtg tttgtgtta 
      atttcaacc gtaaatagt gccaaaatt ctgggtgtt 
      ttccccgtg cctggccgc agccactac atccttgcc 
      agttctctt atgcgagcc cttgctgaa aaagggcac 
      gacgttacg gtcattagc tgtttcggc gaaaaaatt 
      cccccgaaa aatggaacc tacagagac attgtggcg 
      actggagtt ttcgacgaa atgcaaaag gaaatgaac 
      tcgaaaaac atgtccatg ttcgaaatc gggggcgca 
      agcccccta cttaatgct tttttcatg gcagttctg 
      ttgcccgaa atgaccgaa aaggctcta aagcacgaa 
      aatgtccaa aaattcata aactcagac gagaagttt 
      gatgttgtg attgtggag cagtttgca aatgatgca 
      caaaaggcc ttatcgacg cattttggc gccccgttg 
      atcactttc agtacactg ggggccaat tattgggta 
      aatccccta gttggaaac ccctccccg ccttcttat 
      atcccggat ctgatgtcg gactattca gttccaatg 
      acatttgtc gaaagattg gaaaatagt cttgtgtac 
      gttatgaac gagctgctg tacaatttt tacattttt 
      ccgaaacaa aacgaactc atgaaaaag tacatccca 
      agggcccca gctcacatc aatgacgtg ctttacaac 
      agttctatt attttaatg aacgcgcac cccagtatc 
      aaccaacct gtcccatat gtgcccaac atggtcgat 
      atcggaggc tttcacgtc aaacccccc aaaaaacta 
      ccccaagac ttacaagaa tttttagat ggtgccaaa 
      gacggtgtt atttacttc agcatgggt tctaatcta 
      aaaagtgcc gatttgcca aacgacaaa cgcgacgca 
      attcttaaa acgttcgca aaattgaaa caaaaaatt 
      ttgtggaag tgggaagac gaaaatttg ccgggaaaa 
      ccaccaaac gttaaaacc gccaaatgg ttaccccaa 
      caagacatt cttgcacac ccgaatgtt aaacttttt 
      ataacccat ggtggtctt ctaagcacc actgaaacc 
      atctaccac ggagtgccc attctggca gtacctatt 
      ttcggtgac cagaaattg aacgcaagg tctgcggtc 
      aagggtggg tatggagtt catctgcct tatgaagaa 
      ctgaacgaa gaaacttta actaacagt attaacgaa 
      gttttaagt aacaagaag tacaaagaa aatgttcaa 
      aaacggtcg aaactcttc cacgaccgt ctagttagc 
      cctattgac actcttact tactgggtt gagtatgtt 
      gttagacat agaggggca cctcatttg agagttgca 
      gccttggac ctgccttgt tatggtgta aacagtgcc 
      aaaattttg ggcgtaatg cccgtcaca ggccgcagc 
      cattacatc ctcgtaagc accctaatg cgcgccctt 
      gccgaaaaa ggccacgat gtgaccgtt ataagttgt 
      tggggcgaa aaaaatccg cctaaaaat ggcacttac 
      agagacatt gtcgtaact ggggctttt gaagaaatg 
      caaaaactc atgtctggg gccgacatg aatctgttt 
      gatatggat agtcaaaat ccgcttgtt ggtgcaatt 
      tttatggct accatgttc ccagcaatg acggaaata 
      accctaaag catgaaaat gtccaaaag ttgattaac 
      tcgggcgaa aagttcgat gttgtcatt gtggaacag 
      tttgccaat gatgcacaa aaagggctc tcgacgcat 
      tttggggcc cctttggtc agtctcagt ggcgtgggg 
      gcgaactat tgggccaac gctttagtg gggaatcct 
      tcaccccca tcttacatc cccgatatt atgctggac 
      tattcagtc ccaatggcg ttttgtgaa agagttgtt 
      aatagtttt gtttacgtt tttaacgaa cttttacac 
      aagttttat atcttccgt aaacacaac gaaattatg 
      aaaaagtac atcccgaat gcgccagct catatcagt 
      gacgtactt tacaacaat tccattatt ttgatgaac 
      tcccatccc agtatcaac caacctgtc ccatatgtg 
      cccagcatg gtcgatatc ggaggcttt catgtcaaa 
      cccccgaaa aaactacca caagaccta caagaattt 
      ttggatggt gccaaagac ggtgtcatt tacttcagc 
      atgggttct aatttaaaa agtgcagaa ttaccgaac 
      gacaaacgc gacgcaatc cttaaaact ttcgcaaag 
      ttgaaacaa aaagtcctg tggaagtgg gaggaggaa 
      gaccttccg ggaaaatca ccaaatgtt aagactgcg 
      aaatggtta cctcagcaa gatattctt gtaccattt 
      gatttggca ccaaaaatg ctagttaaa atgaaaact 
      acattcttg ctcttggtt ttggtgtta atttcgtgt 
      tatgaagca aacagtgcc aaaattttg gccgttttg 
      cctgtggct ggccggagc cattatatc cttgccagt 
      acccttatg agagcactt gctgagaga gggcacgat 
      gttactgtc attagttgt tttggggaa aagaaccca 
      ccaaaaaat ggaacttac agagacatt gttgtgaca 
      ggagcaatg gaagaatta gagaagcaa atgagttca 
      gacgacgta aatatgttc gaaatggaa ggcataaac 
      ctgttcatt aatgcgctt tttatggct tttatgctg 
      cctacaatg aacgaagcg acactcaaa cacgaaaat 
      gtccaaaaa tttatcaac tctggtgaa aagttcgat 
      gttgtaatt gtggagcaa tttggaaat gatgctgca 
      aaggcccta tcgactcat tttggggcc ccgctgatt 
      agtctcagt gcggtgggg gccagctat tggacaaac 
      cccttggtt ggaaacccg tccccacct tcctatatc 
      ccagacctc atgtccgac tattcggtt cctatgact 
      ttttgcgaa agactcgta aacagtctt gttatcaac 
      caacctgtc ccacacgta cccaacatg atcgaaatt 
      gggggtttt cacatcaaa cccccgaaa aaactacca 
      caagaccta caagaattt ttggatggt gccaaagat 
      ggtatcatt tacttcagc atgggttct aatctaaaa 
      agtgcagat ttaccgaac gacaaacgc gacgcaatt 
      cttaaaact ttcgcaaaa ttgaaacaa aaaatcctg 
      tggaaatgg gaagaggat gatcttccg ggaaaacca 
      ccaaatgtt aaaactgcc aaatggtta ccgcaacaa 
      gaacttctt gtgctaatt tcatattat ggtgtgaaa 
      agtgcaaaa attttggct gtcttgcct gtggcgggc 
      aaaagtcac tacattctt gccagtact ctaatgaga 
      gcacttgct gaaagaggg cacgatgtg actgttata 
      agttgttgg ggggaaaag aaccctcca aaaaatggg 
      acctacagg gacattgtt atgacagga gctatggaa 
      gaattacaa agacacttg agttcaaaa gactccaat 
      atgtttact aggaaaggt gtaaatgta tttgttgat 
      gtttttttt gcgactatg ataatatat agattcaac 
      gaactgaca atgaagcac gaaaatgtg caaaaattt 
      atcagatct ggtgaaaag ttcgacgtc gttattgta 
      gaacagttt gcaaacgac gccctaaag gccttatct 
      acgcatttc ggggcaccg ttgattagt ctcagtgca 
      atgggggcc agctattgg acaaaccct ttggttgga 
      aacccttcc ccaccatct tatattccc catttcagt 
      gctaaaatc ctgggtgtg ttccccgtt cctggccgc 
      agccattac atcctgggc agttcccta atgaaagca 
      cttgctgaa aagggacat gatgtcact gttatcagt 
      tgttttgaa gaacagaat cctccaaaa aacgggact 
      tatcgcgac attgtcata actggagca gttgacgaa 
      atgacaaaa caaatggct aaaatgaac atgttcgat 
      agggaaagc acaaaccca tttgctggc gctgttttc 
      atgtctttt cggttaccg gcaatgacg gaaacgact 
      ctgaagcat gaaaatgtc caaaaactt attaactcg 
      aacgaaaag ttcgatgcc gtgattgtg gagtttgca 
      agcgaggct tttgcaacg agggcaagt cactggatc 
      aaccccttg gttggaaat ccctcccca ccgtcttac 
      attcccgac attatgttg gacttttca gtactcatg 
      acgctttac gaaagactt gtaaacagt cttgtctat 
      gtgtttaat gagctactg ctcaatttc gtcgtttat 
      cccaaacac aacgaacta atgaaaaaa tacattcca 
      aacgcacca tcgcacata agcgaagta ctttacaac 
      cattccatt gttctggta aattcccac ccaagtgtc 
      aaccgaccg gtcccatac gtccccagc atggtcgat 
      attggaggt tttcacatc aaaccaccc aaaaaacta 
      ccacaagac ctccaagaa ttcttagac agtgctaaa 
      cacggtgtt atttatttc agtttaggc tctaatctg 
      aaaagtgca caacttccg cttgagaaa cgtaacgct 
      ctccttcaa actttcgca aaattgaag caaaaaatt 
      ctgtggaag tgggaagat gaggatctt cctggtaaa 
      ccgccaaat gttaaagtt gcgaaatgg ttaccccaa 
      caagacatt cttgcacac ccgaatgtt aaacttttt 
      ataacacat ggtggtcag tccagtacg actgaaacc 
      atctatcat ggggtacca attttggga atacccatt 
      tttggggac caaaaaata aatgcgaaa agtgtagcc 
      agggatggt tgtggtctc tatgtggct tatagtgaa 
      ataactgaa gagaaattg actgaaagt attaacgaa 
      atactgcac aatcaaaag tacaaagaa aatgtccaa 
      aggcgatcg aaacttttc cacgatcgc attgttagt 
      cctttggac acagcgatt tattgggtt gaatatgtc 
      attcggcac aggggggcg cctcatttg agagtggct 
      gctttggac ttaccatg
    mRNA sequence
    • >TC034323 RA
      tcatcatga tttgaacta tcggaagaa attcaagtg 
      gttgtgcta gaattatcg caaattata ttgggcacc 
      ttacgtaaa aaagacaag tttcgttca ttgcgtgaa 
      tgaaaagcc accaaaatg aagcttaca ttatctcta 
      ctagtgttt gtgttaatt tcaaccgta aatagtgcc 
      aaaattctg ggtgttttc cccgtgcct ggccgcagc 
      cactacatc cttgccagt tctcttatg cgagccctt 
      gctgaaaaa gggcacgac gttacggtc attagctgt 
      ttcggcgaa aaaattccc ccgaaaaat ggaacctac 
      agagacatt gtggcgact ggagttttc gacgaaatg 
      caaaaggaa atgaactcg aaaaacatg tccatgttc 
      gaaatcggg ggcgcaagc cccctactt aatgctttt 
      ttcatggca gttctgttg cccgaaatg accgaaaag 
      gctctaaag cacgaaaat gtccaaaaa ttcataaac 
      tcagacgag aagtttgat gttgtgatt gtggagcag 
      tttgcaaat gatgcacaa aaggcctta tcgacgcat 
      tttggcgcc ccgttgatc actttcagt acactgggg 
      gccaattat tgggtaaat cccctagtt ggaaacccc 
      tccccgcct tcttatatc ccggatctg atgtcggac 
      tattcagtt ccaatgaca tttgtcgaa agattggaa 
      aatagtctt gtgtacgtt atgaacgag ctgctgtac 
      aatttttac atttttccg aaacaaaac gaactcatg 
      aaaaagtac atcccaagg gccccagct cacatcaat 
      gacgtgctt tacaacagt tctattatt ttaatgaac 
      gcgcacccc agtatcaac caacctgtc ccatatgtg 
      cccaacatg gtcgatatc ggaggcttt cacgtcaaa 
      ccccccaaa aaactaccc caagactta caagaattt 
      ttagatggt gccaaagac ggtgttatt tacttcagc 
      atgggttct aatctaaaa agtgccgat ttgccaaac 
      gacaaacgc gacgcaatt cttaaaacg ttcgcaaaa 
      ttgaaacaa aaaattttg tggaagtgg gaagacgaa 
      aatttgccg ggaaaacca ccaaacgtt aaaaccgcc 
      aaatggtta ccccaacaa gacattctt gcacacccg 
      aatgttaaa ctttttata acccatggt ggtcttcta 
      agcaccact gaaaccatc taccacgga gtgcccatt 
      ctggcagta cctattttc ggtgaccag aaattgaac 
      gcaaggtct gcggtcaag ggtgggtat ggagttcat 
      ctgccttat gaagaactg aacgaagaa actttaact 
      aacagtatt aacgaagtt ttaagtaac aagaagtac 
      aaagaaaat gttcaaaaa cggtcgaaa ctcttccac 
      gaccgtcta gttagccct attgacact cttacttac 
      tgggttgag tatgttgtt agacataga ggggcacct 
      catttgaga gttgcagcc ttggacctg ccttgttat 
      ggtgtaaac agtgccaaa attttgggc gtaatgccc 
      gtcacaggc cgcagccat tacatcctc gtaagcacc 
      ctaatgcgc gcccttgcc gaaaaaggc cacgatgtg 
      accgttata agttgttgg ggcgaaaaa aatccgcct 
      aaaaatggc acttacaga gacattgtc gtaactggg 
      gcttttgaa gaaatgcaa aaactcatg tctggggcc 
      gacatgaat ctgtttgat atggatagt caaaatccg 
      cttgttggt gcaattttt atggctacc atgttccca 
      gcaatgacg gaaataacc ctaaagcat gaaaatgtc 
      caaaagttg attaactcg ggcgaaaag ttcgatgtt 
      gtcattgtg gaacagttt gccaatgat gcacaaaaa 
      gggctctcg acgcatttt ggggcccct ttggtcagt 
      ctcagtggc gtgggggcg aactattgg gccaacgct 
      ttagtgggg aatccttca cccccatct tacatcccc 
      gatattatg ctggactat tcagtccca atggcgttt 
      tgtgaaaga gttgttaat agttttgtt tacgttttt 
      aacgaactt ttacacaag ttttatatc ttccgtaaa 
      cacaacgaa attatgaaa aagtacatc ccgaatgcg 
      ccagctcat atcagtgac gtactttac aacaattcc 
      attattttg atgaactcc catcccagt atcaaccaa 
      cctgtccca tatgtgccc agcatggtc gatatcgga 
      ggctttcat gtcaaaccc ccgaaaaaa ctaccacaa 
      gacctacaa gaatttttg gatggtgcc aaagacggt 
      gtcatttac ttcagcatg ggttctaat ttaaaaagt 
      gcagaatta ccgaacgac aaacgcgac gcaatcctt 
      aaaactttc gcaaagttg aaacaaaaa gtcctgtgg 
      aagtgggag gaggaagac cttccggga aaatcacca 
      aatgttaag actgcgaaa tggttacct cagcaagat 
      attcttgta ccatttgat ttggcacca aaaatgcta 
      gttaaaatg aaaactaca ttcttgctc ttggttttg 
      gtgttaatt tcgtgttat gaagcaaac agtgccaaa 
      attttggcc gttttgcct gtggctggc cggagccat 
      tatatcctt gccagtacc cttatgaga gcacttgct 
      gagagaggg cacgatgtt actgtcatt agttgtttt 
      ggggaaaag aacccacca aaaaatgga acttacaga 
      gacattgtt gtgacagga gcaatggaa gaattagag 
      aagcaaatg agttcagac gacgtaaat atgttcgaa 
      atggaaggc ataaacctg ttcattaat gcgcttttt 
      atggctttt atgctgcct acaatgaac gaagcgaca 
      ctcaaacac gaaaatgtc caaaaattt atcaactct 
      ggtgaaaag ttcgatgtt gtaattgtg gagcaattt 
      ggaaatgat gctgcaaag gccctatcg actcatttt 
      ggggccccg ctgattagt ctcagtgcg gtgggggcc 
      agctattgg acaaacccc ttggttgga aacccgtcc 
      ccaccttcc tatatccca gacctcatg tccgactat 
      tcggttcct atgactttt tgcgaaaga ctcgtaaac 
      agtcttgtt atcaaccaa cctgtccca cacgtaccc 
      aacatgatc gaaattggg ggttttcac atcaaaccc 
      ccgaaaaaa ctaccacaa gacctacaa gaatttttg 
      gatggtgcc aaagatggt atcatttac ttcagcatg 
      ggttctaat ctaaaaagt gcagattta ccgaacgac 
      aaacgcgac gcaattctt aaaactttc gcaaaattg 
      aaacaaaaa atcctgtgg aaatgggaa gaggatgat 
      cttccggga aaaccacca aatgttaaa actgccaaa 
      tggttaccg caacaagaa cttcttgtg ctaatttca 
      tattatggt gtgaaaagt gcaaaaatt ttggctgtc 
      ttgcctgtg gcgggcaaa agtcactac attcttgcc 
      agtactcta atgagagca cttgctgaa agagggcac 
      gatgtgact gttataagt tgttggggg gaaaagaac 
      cctccaaaa aatgggacc tacagggac attgttatg 
      acaggagct atggaagaa ttacaaaga cacttgagt 
      tcaaaagac tccaatatg tttactagg aaaggtgta 
      aatgtattt gttgatgtt ttttttgcg actatgata 
      atatataga ttcaacgaa ctgacaatg aagcacgaa 
      aatgtgcaa aaatttatc agatctggt gaaaagttc 
      gacgtcgtt attgtagaa cagtttgca aacgacgcc 
      ctaaaggcc ttatctacg catttcggg gcaccgttg 
      attagtctc agtgcaatg ggggccagc tattggaca 
      aaccctttg gttggaaac ccttcccca ccatcttat 
      attccccat ttcagtgct aaaatcctg ggtgtgttc 
      cccgttcct ggccgcagc cattacatc ctgggcagt 
      tccctaatg aaagcactt gctgaaaag ggacatgat 
      gtcactgtt atcagttgt tttgaagaa cagaatcct 
      ccaaaaaac gggacttat cgcgacatt gtcataact 
      ggagcagtt gacgaaatg acaaaacaa atggctaaa 
      atgaacatg ttcgatagg gaaagcaca aacccattt 
      gctggcgct gttttcatg tcttttcgg ttaccggca 
      atgacggaa acgactctg aagcatgaa aatgtccaa 
      aaacttatt aactcgaac gaaaagttc gatgccgtg 
      attgtggag tttgcaagc gaggctttt gcaacgagg 
      gcaagtcac tggatcaac cccttggtt ggaaatccc 
      tccccaccg tcttacatt cccgacatt atgttggac 
      ttttcagta ctcatgacg ctttacgaa agacttgta 
      aacagtctt gtctatgtg tttaatgag ctactgctc 
      aatttcgtc gtttatccc aaacacaac gaactaatg 
      aaaaaatac attccaaac gcaccatcg cacataagc 
      gaagtactt tacaaccat tccattgtt ctggtaaat 
      tcccaccca agtgtcaac cgaccggtc ccatacgtc 
      cccagcatg gtcgatatt ggaggtttt cacatcaaa 
      ccacccaaa aaactacca caagacctc caagaattc 
      ttagacagt gctaaacac ggtgttatt tatttcagt 
      ttaggctct aatctgaaa agtgcacaa cttccgctt 
      gagaaacgt aacgctctc cttcaaact ttcgcaaaa 
      ttgaagcaa aaaattctg tggaagtgg gaagatgag 
      gatcttcct ggtaaaccg ccaaatgtt aaagttgcg 
      aaatggtta ccccaacaa gacattctt gcacacccg 
      aatgttaaa ctttttata acacatggt ggtcagtcc 
      agtacgact gaaaccatc tatcatggg gtaccaatt 
      ttgggaata cccattttt ggggaccaa aaaataaat 
      gcgaaaagt gtagccagg gatggttgt ggtctctat 
      gtggcttat agtgaaata actgaagag aaattgact 
      gaaagtatt aacgaaata ctgcacaat caaaagtac 
      aaagaaaat gtccaaagg cgatcgaaa cttttccac 
      gatcgcatt gttagtcct ttggacaca gcgatttat 
      tgggttgaa tatgtcatt cggcacagg ggggcgcct 
      catttgaga gtggctgct ttggactta ccatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY