General information | |||
TC012034 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 7391630..7402812 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC012034 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01920 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC012034 RA
MAPSHSRQV LIWSIPALA VIFSYFWYR RKRHESKTD PGDKLQSAP ESQQSTKTE TESVTTNRR FSRSLSGVD SAPIDIVIP PKLKAIKSN PVVISDEDL DLEIEKIKS MRYGGSIDM RKSNNSSES SSASKHTPV KKASFDMAQ KVDEEKPLS GTTENAKVD KTISNNNEV QRQNSERDS ANHSPADVM LASPSLSSI SDNHSEGSN DSGKGGSEV VTPPPSRSL VNESTALNI YEFIIPQSL VGKLIGKHG SSVSNIKDK TGAQVLVRK HPTNNKLKV CSIEGTRTE IDSALKLIR DKFPLKRFP EVTLEQVSF LPEVSPVSL VPDHLHLKL IEGINNDTI VSCVVAPNH LFLQQPAHP SFPNLNILT GYMNVCYSE ADSPMLPTP IPENTICAA FSEDSWYRA VVLSTDAES ATAYVKFLD FGGYSYIEI SKLRQIRGD FMSLPFQAA ECFLANVKP VEETGWRDE AFSAVVEMT KGAIIYTQI VDYTEEGIP LVYCYLILG PRQVVFLNQ ELVNQGHAE WLNCGEEAA GVTVA
- >TC012034 RA
atggccccg tcccattcg cgccaagtg ctgatttgg agtatcccc gcgctcgcc gtcattttc agttatttc tggtacaga cggaagaga cacgagtcg aaaactgac cccggtgac aaattgcaa tctgcaccc gaatcgcaa caatcgaca aaaaccgaa accgaaagt gtcacaaca aaccgaagg ttctctcgt tcgctgtca ggcgttgat tccgccccc atcgatatt gttatcccg cccaaactt aaagctatc aaatcaaac cctgttgtt atttccgac gaagactta gacttagaa attgaaaaa attaaatcg atgagatac ggcggctcg attgacatg agaaaatca aataattcc agtgaatca tcgtccgct agtaaacac acgccggtt aagaaggca tcctttgac atggcacag aaggtcgac gaggaaaag ccgctctcc ggcacgacg gaaaatgct aaagtcgat aagacgatc tcaaataat aatgaggtg caaaggcaa aacagcgaa agggattcg gcgaatcat agtccggcg gatgttatg ctggctagt ccgtccctt agcagcatc tccgataat cacagcgag ggttccaac gatagcgga aaagggggc agcgaagtg gtgacacct ccgccgtcc cgatctctg gtcaacgag agcaccgcc ttaaacatt tacgagttc atcattcca caatcgttg gttggtaaa ctaatcggc aaacatggc agttccgtt tcaaacatc aaagacaag accggtgcc caagtactg gtcagaaag cacccaaca aacaataaa ttaaaagtg tgctcgatc gaaggcact cgtaccgag atcgacagc gctttgaaa ctcatacga gataaattt ccattgaaa cggttcccg gaagtcacc cttgagcaa gtctcgttc cttccagaa gtgtcgcct gtttcgctg gttccggac cacttgcat ttgaaactg atcgagggt attaataat gatacgatt gttagttgt gtagttgcg ccaaatcac ttgttctta caacaacca gcacatccg tcgttcccc aaccttaat attctcacc ggttatatg aacgtttgt tactcggag gcggactcg ccaatgctg ccgacgcct attccagaa aacacgatc tgtgccgca ttttctgag gacagttgg tacagagca gtcgtcctg tcgacggat gcggaaagt gcaactgct tatgttaag ttcttagat tttggcggt tattcgtac atcgaaatt tcgaagttg aggcaaatc cggggcgat ttcatgtcg ttaccgttt caagcggcg gaatgtttc ttagcgaac gttaaaccg gtcgaagag acgggctgg cgcgatgag gcgttcagt gcagttgta gaaatgaca aagggggcg atcatctac acccaaata gttgattac actgaggaa ggtattcct ttggtttat tgctattta attttaggt ccaaggcaa gtagtattt ttaaatcag gaattagtg aatcaagga cacgccgaa tggttaaat tgcggggag gaagccgca ggtgttacc gtggcgtaa
- >TC012034 RA
acgttcttc cgccatatt gagaaattt gtggaaaac attgtgagc acgtggaaa gtggtgacc acagttttc cccgttttc aataaaatt ggcaaaact cattgctaa aactgcgat tctgatcgc atctcgaag tgttttctt cgatttcca gcagcgaca acatctaat acaacaacc gtttgtttg gtgaagggg tcaatcgtg gatcgatca agatatggc cccgtccca ttcgcgcca agtgctgat ttggagtat ccccgcgct cgccgtcat tttcagtta tttctggta cagacggaa gagacacga gtcgaaaac tgaccccgg tgacaaatt gcaatctgc acccgaatc gcaacaatc gacaaaaac cgaaaccga aagtgtcac aacaaaccg aaggttctc tcgttcgct gtcaggcgt tgattccgc ccccatcga tattgttat cccgcccaa acttaaagc tatcaaatc aaaccctgt tgttatttc cgacgaaga cttagactt agaaattga aaaaattaa atcgatgag atacggcgg ctcgattga catgagaaa atcaaataa ttccagtga atcatcgtc cgctagtaa acacacgcc ggttaagaa ggcatcctt tgacatggc acagaaggt cgacgagga aaagccgct ctccggcac gacggaaaa tgctaaagt cgataagac gatctcaaa taataatga ggtgcaaag gcaaaacag cgaaaggga ttcggcgaa tcatagtcc ggcggatgt tatgctggc tagtccgtc ccttagcag catctccga taatcacag cgagggttc caacgatag cggaaaagg gggcagcga agtggtgac acctccgcc gtcccgatc tctggtcaa cgagagcac cgccttaaa catttacga gttcatcat tccacaatc gttggttgg taaactaat cggcaaaca tggcagttc cgtttcaaa catcaaaga caagaccgg tgcccaagt actggtcag aaagcaccc aacaaacaa taaattaaa agtgtgctc gatcgaagg cactcgtac cgagatcga cagcgcttt gaaactcat acgagataa atttccatt gaaacggtt cccggaagt cacccttga gcaagtctc gttccttcc agaagtgtc gcctgtttc gctggttcc ggaccactt gcatttgaa actgatcga gggtattaa taatgatac gattgttag ttgtgtagt tgcgccaaa tcacttgtt cttacaaca accagcaca tccgtcgtt ccccaacct taatattct caccggtta tatgaacgt ttgttactc ggaggcgga ctcgccaat gctgccgac gcctattcc agaaaacac gatctgtgc cgcattttc tgaggacag ttggtacag agcagtcgt cctgtcgac ggatgcgga aagtgcaac tgcttatgt taagttctt agattttgg cggttattc gtacatcga aatttcgaa gttgaggca aatccgggg cgatttcat gtcgttacc gtttcaagc ggcggaatg tttcttagc gaacgttaa accggtcga agagacggg ctggcgcga tgaggcgtt cagtgcagt tgtagaaat gacaaaggg ggcgatcat ctacaccca aatagttga ttacactga ggaaggtat tcctttggt ttattgcta tttaatttt aggtccaag gcaagtagt atttttaaa tcaggaatt agtgaatca aggacacgc cgaatggtt aaattgcgg ggaggaagc cgcaggtgt taccgtggc gtaatatgt aaagtaatt ttttgttaa atagtcact tgattggat caagctctt gttgaacgt gaatattat ctagtgtag ttagctgat ttttggaaa agcgtgttt ttatgaatg atattgttg gagaacatt tttacataa cttaggttg tagtttgtt gctttatac cagtttgaa gcgttcggt cgtttcgta catttattt acaagaact tgtttattt ttttaaatt attgtgatg ttttgtttt attgactgc tctattttc agtactaac cttaattgt ttgtgctca attactaat ttaaattta gtgtattgc gctatcgtt tacgtggta gtttagtgt cattattta aactgtaca taaagaatt gtatttttt gataatgtt aatctagct tttgtgtat atttttggg gagcaacta ggtgactgt aggatgatc aatccattt atcttgtat acatgttcc tattattat tattgtttc taccaatgg ttttggtgt ttccattct aagtttatt ttaaaacga actggtttt tcgtacatt tgcactgtt ttcacacag caaacgtat tcgttattt ttaaggatt gatgattct tttttctag tttcgtatt tatgaaata tttgtacat agtatctct tgtactttt tttcttttc ggaagtaaa atataacag atttttttc a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
