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Gene information
General information
TC012034 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7391630..7402812
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012034
    iBeetle screen iB_01920

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012034 RA
      MAPSHSRQV LIWSIPALA VIFSYFWYR RKRHESKTD 
PGDKLQSAP ESQQSTKTE TESVTTNRR FSRSLSGVD 
SAPIDIVIP PKLKAIKSN PVVISDEDL DLEIEKIKS 
MRYGGSIDM RKSNNSSES SSASKHTPV KKASFDMAQ 
KVDEEKPLS GTTENAKVD KTISNNNEV QRQNSERDS 
ANHSPADVM LASPSLSSI SDNHSEGSN DSGKGGSEV 
VTPPPSRSL VNESTALNI YEFIIPQSL VGKLIGKHG 
SSVSNIKDK TGAQVLVRK HPTNNKLKV CSIEGTRTE 
IDSALKLIR DKFPLKRFP EVTLEQVSF LPEVSPVSL 
VPDHLHLKL IEGINNDTI VSCVVAPNH LFLQQPAHP 
SFPNLNILT GYMNVCYSE ADSPMLPTP IPENTICAA 
FSEDSWYRA VVLSTDAES ATAYVKFLD FGGYSYIEI 
SKLRQIRGD FMSLPFQAA ECFLANVKP VEETGWRDE 
AFSAVVEMT KGAIIYTQI VDYTEEGIP LVYCYLILG 
PRQVVFLNQ ELVNQGHAE WLNCGEEAA GVTVA
    CDS sequence
    • >TC012034 RA
      atggccccg tcccattcg cgccaagtg ctgatttgg 
agtatcccc gcgctcgcc gtcattttc agttatttc 
tggtacaga cggaagaga cacgagtcg aaaactgac 
cccggtgac aaattgcaa tctgcaccc gaatcgcaa 
caatcgaca aaaaccgaa accgaaagt gtcacaaca 
aaccgaagg ttctctcgt tcgctgtca ggcgttgat 
tccgccccc atcgatatt gttatcccg cccaaactt 
aaagctatc aaatcaaac cctgttgtt atttccgac 
gaagactta gacttagaa attgaaaaa attaaatcg 
atgagatac ggcggctcg attgacatg agaaaatca 
aataattcc agtgaatca tcgtccgct agtaaacac 
acgccggtt aagaaggca tcctttgac atggcacag 
aaggtcgac gaggaaaag ccgctctcc ggcacgacg 
gaaaatgct aaagtcgat aagacgatc tcaaataat 
aatgaggtg caaaggcaa aacagcgaa agggattcg 
gcgaatcat agtccggcg gatgttatg ctggctagt 
ccgtccctt agcagcatc tccgataat cacagcgag 
ggttccaac gatagcgga aaagggggc agcgaagtg 
gtgacacct ccgccgtcc cgatctctg gtcaacgag 
agcaccgcc ttaaacatt tacgagttc atcattcca 
caatcgttg gttggtaaa ctaatcggc aaacatggc 
agttccgtt tcaaacatc aaagacaag accggtgcc 
caagtactg gtcagaaag cacccaaca aacaataaa 
ttaaaagtg tgctcgatc gaaggcact cgtaccgag 
atcgacagc gctttgaaa ctcatacga gataaattt 
ccattgaaa cggttcccg gaagtcacc cttgagcaa 
gtctcgttc cttccagaa gtgtcgcct gtttcgctg 
gttccggac cacttgcat ttgaaactg atcgagggt 
attaataat gatacgatt gttagttgt gtagttgcg 
ccaaatcac ttgttctta caacaacca gcacatccg 
tcgttcccc aaccttaat attctcacc ggttatatg 
aacgtttgt tactcggag gcggactcg ccaatgctg 
ccgacgcct attccagaa aacacgatc tgtgccgca 
ttttctgag gacagttgg tacagagca gtcgtcctg 
tcgacggat gcggaaagt gcaactgct tatgttaag 
ttcttagat tttggcggt tattcgtac atcgaaatt 
tcgaagttg aggcaaatc cggggcgat ttcatgtcg 
ttaccgttt caagcggcg gaatgtttc ttagcgaac 
gttaaaccg gtcgaagag acgggctgg cgcgatgag 
gcgttcagt gcagttgta gaaatgaca aagggggcg 
atcatctac acccaaata gttgattac actgaggaa 
ggtattcct ttggtttat tgctattta attttaggt 
ccaaggcaa gtagtattt ttaaatcag gaattagtg 
aatcaagga cacgccgaa tggttaaat tgcggggag 
gaagccgca ggtgttacc gtggcgtaa
    mRNA sequence
    • >TC012034 RA
      acgttcttc cgccatatt gagaaattt gtggaaaac 
attgtgagc acgtggaaa gtggtgacc acagttttc 
cccgttttc aataaaatt ggcaaaact cattgctaa 
aactgcgat tctgatcgc atctcgaag tgttttctt 
cgatttcca gcagcgaca acatctaat acaacaacc 
gtttgtttg gtgaagggg tcaatcgtg gatcgatca 
agatatggc cccgtccca ttcgcgcca agtgctgat 
ttggagtat ccccgcgct cgccgtcat tttcagtta 
tttctggta cagacggaa gagacacga gtcgaaaac 
tgaccccgg tgacaaatt gcaatctgc acccgaatc 
gcaacaatc gacaaaaac cgaaaccga aagtgtcac 
aacaaaccg aaggttctc tcgttcgct gtcaggcgt 
tgattccgc ccccatcga tattgttat cccgcccaa 
acttaaagc tatcaaatc aaaccctgt tgttatttc 
cgacgaaga cttagactt agaaattga aaaaattaa 
atcgatgag atacggcgg ctcgattga catgagaaa 
atcaaataa ttccagtga atcatcgtc cgctagtaa 
acacacgcc ggttaagaa ggcatcctt tgacatggc 
acagaaggt cgacgagga aaagccgct ctccggcac 
gacggaaaa tgctaaagt cgataagac gatctcaaa 
taataatga ggtgcaaag gcaaaacag cgaaaggga 
ttcggcgaa tcatagtcc ggcggatgt tatgctggc 
tagtccgtc ccttagcag catctccga taatcacag 
cgagggttc caacgatag cggaaaagg gggcagcga 
agtggtgac acctccgcc gtcccgatc tctggtcaa 
cgagagcac cgccttaaa catttacga gttcatcat 
tccacaatc gttggttgg taaactaat cggcaaaca 
tggcagttc cgtttcaaa catcaaaga caagaccgg 
tgcccaagt actggtcag aaagcaccc aacaaacaa 
taaattaaa agtgtgctc gatcgaagg cactcgtac 
cgagatcga cagcgcttt gaaactcat acgagataa 
atttccatt gaaacggtt cccggaagt cacccttga 
gcaagtctc gttccttcc agaagtgtc gcctgtttc 
gctggttcc ggaccactt gcatttgaa actgatcga 
gggtattaa taatgatac gattgttag ttgtgtagt 
tgcgccaaa tcacttgtt cttacaaca accagcaca 
tccgtcgtt ccccaacct taatattct caccggtta 
tatgaacgt ttgttactc ggaggcgga ctcgccaat 
gctgccgac gcctattcc agaaaacac gatctgtgc 
cgcattttc tgaggacag ttggtacag agcagtcgt 
cctgtcgac ggatgcgga aagtgcaac tgcttatgt 
taagttctt agattttgg cggttattc gtacatcga 
aatttcgaa gttgaggca aatccgggg cgatttcat 
gtcgttacc gtttcaagc ggcggaatg tttcttagc 
gaacgttaa accggtcga agagacggg ctggcgcga 
tgaggcgtt cagtgcagt tgtagaaat gacaaaggg 
ggcgatcat ctacaccca aatagttga ttacactga 
ggaaggtat tcctttggt ttattgcta tttaatttt 
aggtccaag gcaagtagt atttttaaa tcaggaatt 
agtgaatca aggacacgc cgaatggtt aaattgcgg 
ggaggaagc cgcaggtgt taccgtggc gtaatatgt 
aaagtaatt ttttgttaa atagtcact tgattggat 
caagctctt gttgaacgt gaatattat ctagtgtag 
ttagctgat ttttggaaa agcgtgttt ttatgaatg 
atattgttg gagaacatt tttacataa cttaggttg 
tagtttgtt gctttatac cagtttgaa gcgttcggt 
cgtttcgta catttattt acaagaact tgtttattt 
ttttaaatt attgtgatg ttttgtttt attgactgc 
tctattttc agtactaac cttaattgt ttgtgctca 
attactaat ttaaattta gtgtattgc gctatcgtt 
tacgtggta gtttagtgt cattattta aactgtaca 
taaagaatt gtatttttt gataatgtt aatctagct 
tttgtgtat atttttggg gagcaacta ggtgactgt 
aggatgatc aatccattt atcttgtat acatgttcc 
tattattat tattgtttc taccaatgg ttttggtgt 
ttccattct aagtttatt ttaaaacga actggtttt 
tcgtacatt tgcactgtt ttcacacag caaacgtat 
tcgttattt ttaaggatt gatgattct tttttctag 
tttcgtatt tatgaaata tttgtacat agtatctct 
tgtactttt tttcttttc ggaagtaaa atataacag 
atttttttc a
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