Status
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Gene information
General information
TC031237
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012029,TC012030
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7619121..7648328
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031237
    iBeetle screen iB_01919

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031237 RA
      MSIRSFNLI KRNYSQSNS KFFVTTPIY YVNASPHIG 
      HLYSSVIAD ALYRWQQVL GAGKFKFST GTDEHGSKI 
      QQAAHKNNT NPSDYCTRV SSDYKRLSE VFSISYTDF 
      VRTSDEEHK KCVHKFWNV LKNKGKIFS SKYAGWYCI 
      SDESFLTES QLKEKVGPD GRKTIISGE SGHPVEWIE 
      EVNYMFPLG SLQDDVKYW LRSENAVRP AKFQKILLD 
      MVSKGPLPD ISISRPASR VHWGIQVPD DETQTVYVW 
      LDALVNYLT AAGYSHASL HQIWPPSVQ VIGKDILKF 
      HGIYWPSFL IAAGLDPPL SILCHSHWT VDGEKMSKS 
      KNNVVCPFQ RSEIYSSDG LRYFLLREG VAHSDGNYS 
      DTKVIRILN SELADTLGN LLSRCCGTA LNPHQTFPK 
      IEASAFESV ASSDVTKKL IEDVATLPD VCMGHYSNF 
      NFYKAVDRV IATLHQANL FFETFKPWE LKKESAKTR 
      ELDVVLHLT LETLRVCGI LLQPIIPNI ASNLLNKIN 
      VARERRTFS DAKILSWDD TNFVRKELA KDKTLLFKR 
      IVLEGDRKK TKQ
    CDS sequence
    • >TC031237 RA
      atgagcata agaagcttt aatttgatc aaacgaaac 
      tattcgcag tcaaactcg aagtttttc gtaacaacg 
      ccaatttat tacgtaaat gcgagcccc cacattggt 
      catttgtac agttctgtg atcgccgac gcgctttat 
      aggtggcag caggtgctc ggcgcgggt aaatttaaa 
      ttttcaaca ggtaccgac gaacacggt tcaaagatt 
      caacaagcg gctcataaa aacaacaca aatccttcc 
      gattattgt acgagagtg tcgagtgac tataaaagg 
      ttgagtgaa gtgtttagt atcagttac accgatttc 
      gtgaggacc tcggatgag gagcacaag aaatgcgtt 
      cataaattt tggaacgtt ttgaagaat aagggcaaa 
      atcttctcg tcgaaatat gcgggttgg tactgcatt 
      tcagacgag tcgtttcta actgaatcc cagttgaaa 
      gaaaaagtc ggaccggat ggtcgtaaa acaattata 
      tccggggaa agcggtcat ccagtcgag tggatcgag 
      gaggtgaac tatatgttt ccgcttggg tctttgcaa 
      gacgacgtt aaatattgg ctcaggagc gagaatgcg 
      gttagaccg gcaaagttt caaaagatt ttgctggat 
      atggtcagc aagggccct ttaccggac atttcaata 
      tcgaggccg gcgtctcgg gtgcactgg ggcatacaa 
      gtaccggac gacgaaact caaacggtt tacgtatgg 
      ctggacgcc ctcgtcaac tatttaacc gccgccgga 
      tattcgcat gcgagttta caccaaatt tggcctccc 
      agcgtccaa gttatcggc aaggacatt ctcaagttt 
      cacgggatt tattggccc tcttttttg atcgccgct 
      ggtttagat ccccccctg tcgatttta tgtcactcg 
      cattggacc gtcgatggc gaaaaaatg tccaagtcg 
      aaaaacaac gtcgtttgt ccgttccaa agaagtgaa 
      atatactcc agcgacggt ctccgctac tttttactg 
      cgcgaaggt gtcgcacac agtgacggt aattacagc 
      gacacgaaa gtcatccgc atcttgaat tcggaattg 
      gccgatact ttgggtaat ttattgagt cggtgctgc 
      ggaactgcc ctaaatcct catcagacg tttccgaaa 
      atcgaagct tcggccttc gaaagcgtc gcctcatcc 
      gacgtgacg aagaagtta atcgaagac gtcgccacg 
      cttccagac gtttgtatg ggtcattac tctaacttt 
      aacttctat aaggccgtg gatagggta atagcaact 
      cttcatcaa gcgaatttg ttctttgaa actttcaag 
      ccttgggaa ttgaaaaaa gaatcggcc aagacgagg 
      gagttggac gtcgtcttg catttaact ttagaaact 
      ttgagggtt tgcgggatt ttgctgcaa cctattatt 
      ccgaatata gcgtctaat ttattaaat aaaatcaat 
      gtggcgaga gagagacga acttttagc gacgccaag 
      atattatca tgggacgat accaacttc gtccgcaag 
      gaattagcc aaggataag acgctcttg ttcaaacgg 
      atcgttcta gaaggcgac aggaagaaa accaaacaa 
      tga
    mRNA sequence
    • >TC031237 RA
      ttacgtcgg aattacaag ttttattaa aatcattaa 
      ttaaaactc gcagaaatg agcataaga agctttaat 
      ttgatcaaa cgaaactat tcgcagtca aactcgaag 
      tttttcgta acaacgcca atttattac gtaaatgcg 
      agcccccac attggtcat ttgtacagt tctgtgatc 
      gccgacgcg ctttatagg tggcagcag gtgctcggc 
      gcgggtaaa tttaaattt tcaacaggt accgacgaa 
      cacggttca aagattcaa caagcggct cataaaaac 
      aacacaaat ccttccgat tattgtacg agagtgtcg 
      agtgactat aaaaggttg agtgaagtg tttagtatc 
      agttacacc gatttcgtg aggacctcg gatgaggag 
      cacaagaaa tgcgttcat aaattttgg aacgttttg 
      aagaataag ggcaaaatc ttctcgtcg aaatatgcg 
      ggttggtac tgcatttca gacgagtcg tttctaact 
      gaatcccag ttgaaagaa aaagtcgga ccggatggt 
      cgtaaaaca attatatcc ggggaaagc ggtcatcca 
      gtcgagtgg atcgaggag gtgaactat atgtttccg 
      cttgggtct ttgcaagac gacgttaaa tattggctc 
      aggagcgag aatgcggtt agaccggca aagtttcaa 
      aagattttg ctggatatg gtcagcaag ggcccttta 
      ccggacatt tcaatatcg aggccggcg tctcgggtg 
      cactggggc atacaagta ccggacgac gaaactcaa 
      acggtttac gtatggctg gacgccctc gtcaactat 
      ttaaccgcc gccggatat tcgcatgcg agtttacac 
      caaatttgg cctcccagc gtccaagtt atcggcaag 
      gacattctc aagtttcac gggatttat tggccctct 
      tttttgatc gccgctggt ttagatccc cccctgtcg 
      attttatgt cactcgcat tggaccgtc gatggcgaa 
      aaaatgtcc aagtcgaaa aacaacgtc gtttgtccg 
      ttccaaaga agtgaaata tactccagc gacggtctc 
      cgctacttt ttactgcgc gaaggtgtc gcacacagt 
      gacggtaat tacagcgac acgaaagtc atccgcatc 
      ttgaattcg gaattggcc gatactttg ggtaattta 
      ttgagtcgg tgctgcgga actgcccta aatcctcat 
      cagacgttt ccgaaaatc gaagcttcg gccttcgaa 
      agcgtcgcc tcatccgac gtgacgaag aagttaatc 
      gaagacgtc gccacgctt ccagacgtt tgtatgggt 
      cattactct aactttaac ttctataag gccgtggat 
      agggtaata gcaactctt catcaagcg aatttgttc 
      tttgaaact ttcaagcct tgggaattg aaaaaagaa 
      tcggccaag acgagggag ttggacgtc gtcttgcat 
      ttaacttta gaaactttg agggtttgc gggattttg 
      ctgcaacct attattccg aatatagcg tctaattta 
      ttaaataaa atcaatgtg gcgagagag agacgaact 
      tttagcgac gccaagata ttatcatgg gacgatacc 
      aacttcgtc cgcaaggaa ttagccaag gataagacg 
      ctcttgttc aaacggatc gttctagaa ggcgacagg 
      aagaaaacc aaacaatga cttcgtttc tcatttagt 
      attatattt cctcctata ttttattca tgtatgtac 
      agtaacaat tagtttaac atcgataaa gggtataaa 
      acattcact tctctctat atgcaatag attttcgga 
      tagaagttc acatcgatt tagatattt acagaaatc 
      tacacattt ttatacaga tatgctaac cggcgactc 
      aataaatcg cagaaattg tcttgttgt tttatttaa 
      attaaaccc tgtcagtta agtcccgct gtgggggtc 
      cgaacagtc atagaccat ttgaatcgg acactttaa 
      ttcatatat ttagctgca ggatgcacc aatgattcg 
      aaaacagtc aaattagtc cgttattat caagaacac 
      aagcatgaa atttacatc agttccgct caacatcgt 
      ccatcaaat tatgttgaa gtaaaacag ataaaacga 
      aacaaattc ttccataat ttttctctt ttgctcgtt 
      taattggaa acaaatggg gatgaattt tttagaggt 
      tttgttact tcatactta tccacatgt tgcccactt 
      gaagaatac gtagtcaaa tgtttatga aataaaact 
      tacgagtca cgaaataca ttgaaataa acattttta 
      ataaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY