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Gene information
General information
TC034874 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 928426..947373
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034874
    iBeetle screen iB_01915
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_05146
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034874 RA
      MTSKCTVFV FIVLSVTTT VTQSSDITP LVFGKLTDG 
      MPAAFGDFN SDELTDVFV LRDGGNTVE ILLAHEEEP 
      ILREAKPSL SCTFENKLI TSVVPGDFD GDAFMDVMV 
      TLVYRGSEN QHFPHTYAH IIWGGASHL NCSNERAAI 
      PMIGQPLAL DYNQDMIVD LFGQDTDRT RVFWVFNNT 
      RNPPEKIPM EDNRRRTEL SRPHAHAFI DLNEDYMAD 
      LFITTKEDF EIWLGSEHK GRFVYNKTI QHPRDAKII 
      GQSLFMDVE LKGQMDLVT PVCYDERCQ NSTLLVYSN 
      GEWHNLQVN FKDDYGALW GFALKPGSK YTDTITLHS 
      GDFNMDGYP DILATLSPN NPEHPQSFL LENVPCMTS 
      CGKFKRSYS VRWNILNSF KNGTAMAAF FDFYQDGIL 
      DVILVTYHN KEYKAAAFK NSLDYDANF IKVMVLTGL 
      SNKNNQMIM GRVGKKRRT YGTNLPGPL ISYKTTTQE 
      GYTRHGASA QLPQSAHFS LNLPYTIFG LGRTPNFVD 
      SLTVGLSNH SRSWTQIIP NSQMVVIPW PANEPSRWK 
      AQLFVTPSK LILMSVAAL TGICGIITV IIGVLHWKE 
      RQEDKKERL SESHRFHFD AILEEGMEK KGSRLVQPA 
      AKGLASDSK KSQDVASGK LWPRSGRRR ESAGGSLSA 
      KGESTRKPP PQRSKTYDI KRPRPRGAF NPGPAVGRT 
      ETTRLDEEF EAELGSVFL PGSKKQSLN HLLNFVYAT 
      PSREGRYQC AERGNKASN RLILTRKHK YNKEHFLQA 
      NCQFVVNTS GDYKQYMNN PDALVDWSL IEQVNVQVS 
      DYPSCPICL YPPVAAKMT RCGHIYCWS CILHYLSLS 
      DKTWRKCPI CFESIHKQD LKSVVSKHY KTFNVDDVI 
      TFKLMKRPK GSLVTYPAS AVVKDEEII KFSDSEIDV 
      YSKLLIANQ AEILSIIDR ENDELNVQL VENGDCPEN 
      CFIEEALTY LSQRRDTIL NSSGNEIVT LEEALDNLQ 
      LSDNNDETF DNNNIKAEN LDISTNQQS KLHYFYQAS 
      DGQHIYLHA INARMLEYT YGSLENSPE ILSGKILEK 
      EGGSMTEEL RKRLRYLQH LPVTCQFEV AEIQLKQPI 
      VNKETLEHF NEQIEVRRK RRQRRAREE KRREKKIAD 
      EENRLMGKY PVPNLHIES ESQFPCFDG RIRTESECT 
      QPSEGSSCE TALTPPSSL TIENEHAGP SFAKMLASA 
      KKTETNNWP SLKATTAPT KLINVTGQK INSSCFNVI 
      RNRSDSEAE TEDFEPIPP STSLGDAIV QALQKAEEN 
      DVFTESGSQ PVGKKKKIK NRQKVLFTT TLAYTGK
    CDS sequence
    • >TC034874 RA
      atgacctcc aaatgcacc gtttttgtc ttcatcgtg 
      ttgtccgtc accaccact gtaacccaa agtagcgac 
      ataacaccc ctagttttt ggaaaattg accgatggc 
      atgcctgcc gcatttggc gatttcaat tccgacgaa 
      ctgaccgat gttttcgtc ctgcgagac ggtggaaac 
      accgttgaa attctgcta gcgcacgag gaagagccg 
      atattgcga gaggctaaa cctagcttg tcttgcact 
      tttgaaaat aaactgatc acaagtgtg gtgcctgga 
      gacttcgac ggtgacgcc ttcatggac gttatggtc 
      actctcgtg tacagagga agcgaaaat cagcatttt 
      ccgcacact tacgcccac attatctgg ggtggggct 
      agtcatctg aactgcagt aatgaaaga gcggcgatt 
      cctatgatc ggacaaccc ttagcgctt gactataat 
      caggacatg attgtagat ttgtttgga caggataca 
      gatagaacc agggtattc tgggttttc aataatacg 
      agaaatcca ccggaaaag attccaatg gaggataat 
      agacgcagg acggagttg agcagaccc cacgctcat 
      gcttttatt gatttaaat gaggactac atggccgac 
      ttgttcata acaacaaaa gaagacttt gaaatttgg 
      ctcggaagc gagcataag ggccgattt gtctacaac 
      aaaaccatc caacaccca agagatgct aaaataatc 
      ggccaatct ctctttatg gacgtcgaa ctgaaaggc 
      caaatggat ttagtaacg ccagtttgc tacgacgaa 
      aggtgtcaa aacagcaca cttttggtt tacagtaac 
      ggcgagtgg cacaacttg caagtgaat ttcaaagac 
      gactatggg gccttgtgg ggctttgcc ctcaaaccg 
      ggcagcaaa tacaccgac accatcact ttgcacagt 
      ggcgatttt aacatggac ggatacccg gatattttg 
      gccaccctg agccccaat aacccggag cacccgcaa 
      tcgttctta cttgaaaac gtcccctgc atgaccagc 
      tgtggcaaa tttaagcgc tcatacagc gtaaggtgg 
      aacattttg aactcgttt aagaacgga accgcaatg 
      gctgctttc ttcgacttc taccaagac ggtattttg 
      gacgtgatc ttggtcact taccacaat aaagagtat 
      aaagctgcg gcgttcaaa aatagtctt gattatgac 
      gccaacttc atcaaagtc atggttctg accgggctt 
      tcgaacaaa aacaatcag atgattatg gggagagtg 
      gggaagaaa cggcgcact tacggcact aatttgccg 
      ggacctttg atttcttac aagactaca acgcaagaa 
      ggctacaca cgacatggt gcttcagcc cagctacct 
      caatcagcc catttcagt ctgaactta ccgtacacc 
      atttttggt ttagggaga accccaaat tttgttgac 
      tcgttaacg gttggtttg tccaaccat tcgcgatcg 
      tggacccaa atcatacca aactcccag atggtagta 
      ataccatgg cctgcgaac gaaccctcg cgatggaag 
      gcacagttg tttgtgact cccagtaaa ctcattcta 
      atgtctgtg gcggccttg actggcatt tgtggtatc 
      ataactgtg ataattgga gtcttgcat tggaaggag 
      cggcaagag gataagaag gagcggctt tccgagtct 
      catcgtttt catttcgat gctatactg gaagaagga 
      atggagaaa aaagggagt cgtttagta cagcctgcc 
      gccaagggg cttgcttct gactccaaa aaaagtcaa 
      gatgttgcc agtggtaaa ctgtggccc cgttcgggc 
      cgccgccgc gagtccgct ggcggcagt ttatctgcc 
      aaaggcgaa tcaactcgc aaaccgccc ccacaacgt 
      tcgaaaacc tacgatatc aaaagacct aggccgcgg 
      ggagccttc aaccccggt cctgctgtg ggtcgaacg 
      gagacaacc cggctagat gaggaattt gaagctgaa 
      ttgggttca gtttttcta ccaggcagt aaaaaacaa 
      agcctcaac catttacta aatttcgtt tatgcgact 
      ccttcacga gaaggacga tatcaatgt gctgaaaga 
      ggcaataaa gcatccaat cgtttgata ctcaccagg 
      aagcacaaa tacaacaag gaacacttc ctgcaagca 
      aattgccaa tttgttgtc aatacttcg ggtgactat 
      aagcaatat atgaacaac cctgatgcc ttagttgat 
      tggagtctt atagaacaa gtgaatgta caagtgtct 
      gattaccca tcatgccca atctgtctt tatcctcca 
      gttgctgcc aaaatgaca agatgtggt catatttac 
      tgctggtct tgtattcta cattactta tcgctttct 
      gataaaacg tggcgtaaa tgtccgatt tgtttcgaa 
      tcgattcat aaacaagat ttgaaaagt gtggttagt 
      aaacattac aaaacgttt aatgttgat gatgtaatt 
      acgttcaaa ttgatgaaa cgaccgaaa ggttctttg 
      gttacttat cctgcatcg gctgtcgtc aaagatgaa 
      gaaattatt aaattttct gattcagaa atcgatgtt 
      tattctaaa ttactgata gcgaatcag gcggaaatt 
      ttgtcaatt attgataga gaaaatgat gaattaaac 
      gtgcaacta gtggaaaat ggtgactgt cctgagaat 
      tgtttcata gaagaagct ctaacatat ttgagtcaa 
      cggagagac acaattttg aattcaagt ggcaatgaa 
      atagttacc cttgaggaa gctttagat aatttgcaa 
      ttaagcgac aacaacgat gaaactttt gataataat 
      aacatcaaa gccgaaaat ttggacata tcgaccaac 
      cagcagtca aaattgcat tacttctat caagcatct 
      gacggccaa catatctat cttcacgct atcaatgca 
      cgaatgctc gaatacacg tacgggtcg ttggaaaac 
      agtcctgag atattgagt ggaaaaatc cttgaaaaa 
      gaaggagga tccatgact gaagagttg agaaaacga 
      ttgagatat ctccaacat ttgcccgtc acttgccaa 
      ttcgaggtt gcagaaata cagttgaaa caaccaatt 
      gtcaataaa gaaactttg gaacatttt aacgaacaa 
      attgaagtg cgtcgtaag cggcgccag cgtcgcgct 
      cgtgaagaa aaacgccgc gagaaaaaa atcgccgac 
      gaggaaaat cgtctcatg ggtaaatac cctgtaccc 
      aacttacac atcgagtca gagtcgcaa tttccttgt 
      ttcgatgga agaataaga acagaatcg gaatgcact 
      caaccgagt gaaggaagt tcgtgtgag acagcacta 
      acacctcca agtagtctc actattgaa aatgaacat 
      gctggtcct tcttttgct aaaatgttg gcaagtgct 
      aagaaaacc gaaactaac aattggcca tcacttaaa 
      gctacaact gccccaact aaattaatt aacgtcact 
      ggccaaaaa attaactcg agctgtttt aacgtgatt 
      cgtaatcgt agtgacagt gaagcggaa acggaagat 
      ttcgagccg attccaccg agcacaagt ttgggtgat 
      gcgattgtt caagcgttg cagaaagcg gaggaaaat 
      gatgtattt accgagtct ggttcgcaa ccagttgga 
      aaaaagaag aagattaaa aacaggcaa aaggttctc 
      ttcactacc actctggct tataccggg aaataa
    mRNA sequence
    • >TC034874 RA
      attgacagc atgacttct gtcaccgaa tgtcaaaat 
      aatgcgaaa taacgcgaa ttttccaaa caaaaacaa 
      tatttaagt gttttgtgc cccctatca agttataaa 
      ctgctccgg gatgacctc caaatgcac cgtttttgt 
      cttcatcgt gttgtccgt caccaccac tgtaaccca 
      aagtagcga cataacacc cctagtttt tggaaaatt 
      gaccgatgg catgcctgc cgcatttgg cgatttcaa 
      ttccgacga actgaccga tgttttcgt cctgcgaga 
      cggtggaaa caccgttga aattctgct agcgcacga 
      ggaagagcc gatattgcg agaggctaa acctagctt 
      gtcttgcac ttttgaaaa taaactgat cacaagtgt 
      ggtgcctgg agacttcga cggtgacgc cttcatgga 
      cgttatggt cactctcgt gtacagagg aagcgaaaa 
      tcagcattt tccgcacac ttacgccca cattatctg 
      gggtggggc tagtcatct gaactgcag taatgaaag 
      agcggcgat tcctatgat cggacaacc cttagcgct 
      tgactataa tcaggacat gattgtaga tttgtttgg 
      acaggatac agatagaac cagggtatt ctgggtttt 
      caataatac gagaaatcc accggaaaa gattccaat 
      ggaggataa tagacgcag gacggagtt gagcagacc 
      ccacgctca tgcttttat tgatttaaa tgaggacta 
      catggccga cttgttcat aacaacaaa agaagactt 
      tgaaatttg gctcggaag cgagcataa gggccgatt 
      tgtctacaa caaaaccat ccaacaccc aagagatgc 
      taaaataat cggccaatc tctctttat ggacgtcga 
      actgaaagg ccaaatgga tttagtaac gccagtttg 
      ctacgacga aaggtgtca aaacagcac acttttggt 
      ttacagtaa cggcgagtg gcacaactt gcaagtgaa 
      tttcaaaga cgactatgg ggccttgtg gggctttgc 
      cctcaaacc gggcagcaa atacaccga caccatcac 
      tttgcacag tggcgattt taacatgga cggataccc 
      ggatatttt ggccaccct gagccccaa taacccgga 
      gcacccgca atcgttctt acttgaaaa cgtcccctg 
      catgaccag ctgtggcaa atttaagcg ctcatacag 
      cgtaaggtg gaacatttt gaactcgtt taagaacgg 
      aaccgcaat ggctgcttt cttcgactt ctaccaaga 
      cggtatttt ggacgtgat cttggtcac ttaccacaa 
      taaagagta taaagctgc ggcgttcaa aaatagtct 
      tgattatga cgccaactt catcaaagt catggttct 
      gaccgggct ttcgaacaa aaacaatca gatgattat 
      ggggagagt ggggaagaa acggcgcac ttacggcac 
      taatttgcc gggaccttt gatttctta caagactac 
      aacgcaaga aggctacac acgacatgg tgcttcagc 
      ccagctacc tcaatcagc ccatttcag tctgaactt 
      accgtacac catttttgg tttagggag aaccccaaa 
      ttttgttga ctcgttaac ggttggttt gtccaacca 
      ttcgcgatc gtggaccca aatcatacc aaactccca 
      gatggtagt aataccatg gcctgcgaa cgaaccctc 
      gcgatggaa ggcacagtt gtttgtgac tcccagtaa 
      actcattct aatgtctgt ggcggcctt gactggcat 
      ttgtggtat cataactgt gataattgg agtcttgca 
      ttggaagga gcggcaaga ggataagaa ggagcggct 
      ttccgagtc tcatcgttt tcatttcga tgctatact 
      ggaagaagg aatggagaa aaaagggag tcgtttagt 
      acagcctgc cgccaaggg gcttgcttc tgactccaa 
      aaaaagtca agatgttgc cagtggtaa actgtggcc 
      ccgttcggg ccgccgccg cgagtccgc tggcggcag 
      tttatctgc caaaggcga atcaactcg caaaccgcc 
      cccacaacg ttcgaaaac ctacgatat caaaagacc 
      taggccgcg gggagcctt caaccccgg tcctgctgt 
      gggtcgaac ggagacaac ccggctaga tgaggaatt 
      tgaagctga attgggttc agtttttct accaggcag 
      taaaaaaca aagcctcaa ccatttact aaatttcgt 
      ttatgcgac tccttcacg agaaggacg atatcaatg 
      tgctgaaag aggcaataa agcatccaa tcgtttgat 
      actcaccag gaagcacaa atacaacaa ggaacactt 
      cctgcaagc aaattgcca atttgttgt caatacttc 
      gggtgacta taagcaata tatgaacaa ccctgatgc 
      cttagttga ttggagtct tatagaaca agtgaatgt 
      acaagtgtc tgattaccc atcatgccc aatctgtct 
      ttatcctcc agttgctgc caaaatgac aagatgtgg 
      tcatattta ctgctggtc ttgtattct acattactt 
      atcgctttc tgataaaac gtggcgtaa atgtccgat 
      ttgtttcga atcgattca taaacaaga tttgaaaag 
      tgtggttag taaacatta caaaacgtt taatgttga 
      tgatgtaat tacgttcaa attgatgaa acgaccgaa 
      aggttcttt ggttactta tcctgcatc ggctgtcgt 
      caaagatga agaaattat taaattttc tgattcaga 
      aatcgatgt ttattctaa attactgat agcgaatca 
      ggcggaaat tttgtcaat tattgatag agaaaatga 
      tgaattaaa cgtgcaact agtggaaaa tggtgactg 
      tcctgagaa ttgtttcat agaagaagc tctaacata 
      tttgagtca acggagaga cacaatttt gaattcaag 
      tggcaatga aatagttac ccttgagga agctttaga 
      taatttgca attaagcga caacaacga tgaaacttt 
      tgataataa taacatcaa agccgaaaa tttggacat 
      atcgaccaa ccagcagtc aaaattgca ttacttcta 
      tcaagcatc tgacggcca acatatcta tcttcacgc 
      tatcaatgc acgaatgct cgaatacac gtacgggtc 
      gttggaaaa cagtcctga gatattgag tggaaaaat 
      ccttgaaaa agaaggagg atccatgac tgaagagtt 
      gagaaaacg attgagata tctccaaca tttgcccgt 
      cacttgcca attcgaggt tgcagaaat acagttgaa 
      acaaccaat tgtcaataa agaaacttt ggaacattt 
      taacgaaca aattgaagt gcgtcgtaa gcggcgcca 
      gcgtcgcgc tcgtgaaga aaaacgccg cgagaaaaa 
      aatcgccga cgaggaaaa tcgtctcat gggtaaata 
      ccctgtacc caacttaca catcgagtc agagtcgca 
      atttccttg tttcgatgg aagaataag aacagaatc 
      ggaatgcac tcaaccgag tgaaggaag ttcgtgtga 
      gacagcact aacacctcc aagtagtct cactattga 
      aaatgaaca tgctggtcc ttcttttgc taaaatgtt 
      ggcaagtgc taagaaaac cgaaactaa caattggcc 
      atcacttaa agctacaac tgccccaac taaattaat 
      taacgtcac tggccaaaa aattaactc gagctgttt 
      taacgtgat tcgtaatcg tagtgacag tgaagcgga 
      aacggaaga tttcgagcc gattccacc gagcacaag 
      tttgggtga tgcgattgt tcaagcgtt gcagaaagc 
      ggaggaaaa tgatgtatt taccgagtc tggttcgca 
      accagttgg aaaaaagaa gaagattaa aaacaggca 
      aaaggttct cttcactac cactctggc ttataccgg 
      gaaataata tacgtattg ataggtgta cattttttt 
      gaaactgct gcataaatt catttttgc tttaattat 
      tcattattt aatttggtc tttatgatg ggtgttaat 
      aattttgtg gcccttgac tcgtggaca agaggattt 
      tttctatca acttttaac aatgtgtac caccggtgc 
      ggttttaac aatgtccag ggttataca ggggtttgt 
      tgttttata cattatt