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Gene information
General information
TC011991 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 1340206..1345716
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011991
    iBeetle screen iB_01913
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    elytra partially blistered - Number of animals showing the phenotype: 7

    Adult morphology
    elytra partially blistered - Number of animals showing the phenotype: 4


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011991 RA
      MFEKKTRLH VKSLLEKQF DNFRELEQS VKNHLIDTS 
      LGIKQTEKV SSNRSTNER NMTRKTRTR RPRIHSIFE 
      SGYCAVCHT PYNSLEEHI QSKRHLKLI GDDGNFISI 
      KGFDTFLAL DGIDAIEVN EKTRDYSPP RRRSRMPRT 
      RTSSVMCDR IKDSPMSPV GSDSGHHLR SRKNINYMT 
      PPLEEDSLT EKPEVVKEY RELRSSSRL LAKLADQMV 
      ENDEVWNSG RPKRSCNRT RISADERLV VDNKSYYKV 
      EVLSSKLRS GVTHFQEVK RPETPVEKE DDKGLIVKF 
      RKLRSSELV RLNNEAENF LFPKKDETS DDDDDDDKT 
      KNDNSSVSE TDDSKVKIE DDESMHSTS SESKKKRRR 
      THAEAFIMD NQKYYKFET PGSRLRYHG SYLSPVPMK 
      SKNNGDCVV KNESEESEP DKNFNLRLD DYNFSFESV 
      PKNEAWYQT FQRRDKGEE KYVFLSDDN YWDPLILPY 
      QLDYVPPLD PQECVSTYN QIKKCLMEP PSASTSVTC 
      STPDPNASN SSVETPFNQ DEDSKISAT EISSNSTLD 
      EIKPSRNKR SRRSGMVVN VTGTGKNPR KSPRQHAST 
      LAILSSLIQ QRKRKSRRT DLDFRSNLP TILEEPKPT 
      KSPKKSKVD YFSMMKRVE EEFASACDD NLDVEIEKD 
      EDIAFKGQV GVEDILNVY EDNKDTIVK SPKRLVSAK 
      PGRKPGKRK KNRTGWPLN RSRRMRRKE DDDSTVDSL 
      SLNADSDGC DDTTDSIKK KSNCSENNN QNDQSSLSD 
      VNIGDSNCD LSNADRVKN ENRSDDANS VLSNKVTSD 
      GFNIDLQYQ PYVRVQKLD KNHIDNKSE PAKRSNSPK 
      RTNRGQRRM PASPKSPRM LRRPRGRWY RER
    CDS sequence
    • >TC011991 RA
      atgtttgaa aagaaaacc aggttgcat gttaaaagt 
      ttgttggaa aaacagttt gacaatttt cgcgaattg 
      gaacagtcc gtaaaaaat catttaata gacacttcg 
      ttaggtatt aaacaaact gaaaaagtt tcctcaaat 
      cgctctaca aatgagcgt aacatgaca cgtaagact 
      agaacaaga cggccacgc atccattct atattcgag 
      tctggttat tgtgctgtt tgtcacaca ccctataac 
      agccttgag gaacacatc cagtccaaa agacattta 
      aaactcatc ggcgacgat ggcaatttc atatcaata 
      aaaggattc gatacgttt ctggccttg gatggaatt 
      gacgccatc gaagtgaat gaaaaaact cgcgactat 
      tctccgccc cgccgccgc tccaggatg cctcgaacg 
      cgcacctcg tccgtgatg tgcgaccga ataaaagac 
      tctccgatg tcccccgtg ggcagcgac tcaggacac 
      catttacgt tcaagaaaa aacataaat tatatgacg 
      cctcctctc gaagaagac agtctaact gagaaaccc 
      gaagtcgtg aaggaatat cgcgaatta agatccagt 
      tcccgtctt ttggccaaa ctcgccgat caaatggtc 
      gaaaatgac gaagtgtgg aattccggt cggccgaag 
      cgatcctgc aaccgaacg aggatttcg gccgacgaa 
      cgattagtc gttgataat aaaagttat tacaaagtc 
      gaagttttg tcgtcgaaa cttcgttca ggagttact 
      cattttcaa gaagttaaa cggccggaa acaccggtc 
      gagaaagaa gatgataaa ggattgata gtaaagttt 
      aggaagttg agaagttca gaactcgtc cgattgaat 
      aacgaagcg gagaatttt ctgtttccg aaaaaggac 
      gaaacgagt gacgacgac gacgatgat gataagacg 
      aagaatgac aatagttcg gtcagcgaa accgatgat 
      agtaaagtt aaaattgaa gatgacgag tcgatgcac 
      agtacgagt tccgaaagc aaaaagaag aggaggcga 
      acgcacgcc gaggcgttt ataatggat aatcaaaaa 
      tactacaag tttgaaact cccggttca aggttgagg 
      taccacggt tcatatctt tctcctgtt cctatgaaa 
      tcgaaaaat aatggagat tgcgtggtg aaaaacgaa 
      tcggaagag tccgaacct gataaaaac tttaacctg 
      agattagat gattacaac ttttcgttt gagagcgtc 
      cccaaaaac gaggcctgg tatcaaacg tttcagagg 
      cgcgacaag ggggaagag aaatacgtt tttttaagt 
      gacgacaat tattgggat ccgttgatt cttccctac 
      caactggat tacgtaccg cctctggac ccgcaagag 
      tgtgtctcg acctacaac cagatcaaa aaatgcctt 
      atggagcct ccgtcagcg tcaacttcg gtcacatgc 
      agcaccccc gatcccaac gcaagtaat tcaagcgtt 
      gaaactccc ttcaatcaa gacgaagat agtaaaata 
      agtgccact gaaattagt tcaaatagt actttggat 
      gagattaaa ccgtctcga aataagcga agccgacgc 
      tcgggaatg gtcgtgaat gtgaccgga actggcaaa 
      aatccccgg aaatcgcct cgacaacat gcctccact 
      ctcgcgatt ttgagtagt ttgatacag cagcggaaa 
      cgaaagtcg cggcgaact gatcttgac ttccggtct 
      aacctacca acgattttg gaggaacca aaaccgacg 
      aaatcgccg aagaagtcc aaagttgat tacttttcg 
      atgatgaag agggtcgag gaggagttt gcgagtgcg 
      tgcgacgat aatttggat gtggagatc gaaaaggac 
      gaagacatc gcgttcaag ggccaggtg ggagtggag 
      gatattttg aacgtatac gaggataac aaagacact 
      attgttaag tcgcctaag aggcttgta agtgcgaaa 
      ccgggccgg aagccaggc aagaggaaa aaaaatcgt 
      acagggtgg cctttaaac aggagcagg agaatgagg 
      aggaaggag gatgatgat agcacggtg gattcgttg 
      agcttgaat gcggattcg gacggatgt gacgatacg 
      actgatagt attaagaag aagtcgaac tgtagtgaa 
      aacaataac caaaatgac caaagtagt ttaagtgat 
      gtaaatata ggcgatagt aattgtgat ttaagtaac 
      gccgacagg gttaaaaac gaaaacaga agtgatgat 
      gcgaactca gtactatca aataaagtg actagtgac 
      ggttttaat attgatttg caatatcag ccgtatgtg 
      cgcgtgcaa aaacttgat aagaatcac atagacaat 
      aaaagcgaa ccggcaaag aggagtaat tcaccgaag 
      cgaacaaac cggggacag aggaggatg ccggcgtcg 
      ccgaaatca ccgagaatg ttgagaaga ccgagaggg 
      cgctggtat cgagagaga taa
    mRNA sequence
    • >TC011991 RA
      cgaaacttc attgtggcg gattcccaa atgttttga 
      aattgaggt aattcgcgt ttttagctt gtgtctttt 
      gaacaaagt gtgtacgtg tgcattggg gagtttgaa 
      gcgtccgtg aagccgcaa agcgtttaa aatgaggga 
      aaagtcttg actgattct cggaaaagt ccagtaaac 
      accattgaa gttcgaatg ggcaacgta atacagtat 
      gtaactctt caagcaatg tcaaccata aacttacct 
      gtggaattt ttttagatt atttttggt gatgccatg 
      tttgaaaag aaaaccagg ttgcatgtt aaaagtttg 
      ttggaaaaa cagtttgac aattttcgc gaattggaa 
      cagtccgta aaaaatcat ttaatagac acttcgtta 
      ggtattaaa caaactgaa aaagtttcc tcaaatcgc 
      tctacaaat gagcgtaac atgacacgt aagactaga 
      acaagacgg ccacgcatc cattctata ttcgagtct 
      ggttattgt gctgtttgt cacacaccc tataacagc 
      cttgaggaa cacatccag tccaaaaga catttaaaa 
      ctcatcggc gacgatggc aatttcata tcaataaaa 
      ggattcgat acgtttctg gccttggat ggaattgac 
      gccatcgaa gtgaatgaa aaaactcgc gactattct 
      ccgccccgc cgccgctcc aggatgcct cgaacgcgc 
      acctcgtcc gtgatgtgc gaccgaata aaagactct 
      ccgatgtcc cccgtgggc agcgactca ggacaccat 
      ttacgttca agaaaaaac ataaattat atgacgcct 
      cctctcgaa gaagacagt ctaactgag aaacccgaa 
      gtcgtgaag gaatatcgc gaattaaga tccagttcc 
      cgtcttttg gccaaactc gccgatcaa atggtcgaa 
      aatgacgaa gtgtggaat tccggtcgg ccgaagcga 
      tcctgcaac cgaacgagg atttcggcc gacgaacga 
      ttagtcgtt gataataaa agttattac aaagtcgaa 
      gttttgtcg tcgaaactt cgttcagga gttactcat 
      tttcaagaa gttaaacgg ccggaaaca ccggtcgag 
      aaagaagat gataaagga ttgatagta aagtttagg 
      aagttgaga agttcagaa ctcgtccga ttgaataac 
      gaagcggag aattttctg tttccgaaa aaggacgaa 
      acgagtgac gacgacgac gatgatgat aagacgaag 
      aatgacaat agttcggtc agcgaaacc gatgatagt 
      aaagttaaa attgaagat gacgagtcg atgcacagt 
      acgagttcc gaaagcaaa aagaagagg aggcgaacg 
      cacgccgag gcgtttata atggataat caaaaatac 
      tacaagttt gaaactccc ggttcaagg ttgaggtac 
      cacggttca tatctttct cctgttcct atgaaatcg 
      aaaaataat ggagattgc gtggtgaaa aacgaatcg 
      gaagagtcc gaacctgat aaaaacttt aacctgaga 
      ttagatgat tacaacttt tcgtttgag agcgtcccc 
      aaaaacgag gcctggtat caaacgttt cagaggcgc 
      gacaagggg gaagagaaa tacgttttt ttaagtgac 
      gacaattat tgggatccg ttgattctt ccctaccaa 
      ctggattac gtaccgcct ctggacccg caagagtgt 
      gtctcgacc tacaaccag atcaaaaaa tgccttatg 
      gagcctccg tcagcgtca acttcggtc acatgcagc 
      acccccgat cccaacgca agtaattca agcgttgaa 
      actcccttc aatcaagac gaagatagt aaaataagt 
      gccactgaa attagttca aatagtact ttggatgag 
      attaaaccg tctcgaaat aagcgaagc cgacgctcg 
      ggaatggtc gtgaatgtg accggaact ggcaaaaat 
      ccccggaaa tcgcctcga caacatgcc tccactctc 
      gcgattttg agtagtttg atacagcag cggaaacga 
      aagtcgcgg cgaactgat cttgacttc cggtctaac 
      ctaccaacg attttggag gaaccaaaa ccgacgaaa 
      tcgccgaag aagtccaaa gttgattac ttttcgatg 
      atgaagagg gtcgaggag gagtttgcg agtgcgtgc 
      gacgataat ttggatgtg gagatcgaa aaggacgaa 
      gacatcgcg ttcaagggc caggtggga gtggaggat 
      attttgaac gtatacgag gataacaaa gacactatt 
      gttaagtcg cctaagagg cttgtaagt gcgaaaccg 
      ggccggaag ccaggcaag aggaaaaaa aatcgtaca 
      gggtggcct ttaaacagg agcaggaga atgaggagg 
      aaggaggat gatgatagc acggtggat tcgttgagc 
      ttgaatgcg gattcggac ggatgtgac gatacgact 
      gatagtatt aagaagaag tcgaactgt agtgaaaac 
      aataaccaa aatgaccaa agtagttta agtgatgta 
      aatataggc gatagtaat tgtgattta agtaacgcc 
      gacagggtt aaaaacgaa aacagaagt gatgatgcg 
      aactcagta ctatcaaat aaagtgact agtgacggt 
      tttaatatt gatttgcaa tatcagccg tatgtgcgc 
      gtgcaaaaa cttgataag aatcacata gacaataaa 
      agcgaaccg gcaaagagg agtaattca ccgaagcga 
      acaaaccgg ggacagagg aggatgccg gcgtcgccg 
      aaatcaccg agaatgttg agaagaccg agagggcgc 
      tggtatcga gagagataa ttagatttc catgttatt 
      tattttctt gtattcttt aagttaaac atgtattcg 
      aatattttg taccaaatc tgtattatt ttaaaactg 
      aagaatcga ttatttttt tctaatgtt taataatta 
      tagtttttg catcttgga tttgttttt tttgtatat 
      gtaaacata catttacct accatagaa gcatgaatt 
      tttgcgcca gtactgtac cgagtacta catgtaatt 
      tcgttggga aagaaaata ttacatttc aagacaaat 
      tttaaataa aattgttgt ttacaa