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Gene information
General information
TC011991 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9144649..9150159
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011991
    iBeetle screen iB_01913

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    Pupal morphology
    elytra partially blistered - Number of animals showing the phenotype: 7

    Adult morphology
    elytra partially blistered - Number of animals showing the phenotype: 4


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011991 RA
      MFEKKTRLH VKSLLEKQF DNFRELEQS VKNHLIDTS 
LGIKQTEKV SSNRSTNER NMTRKTRTR RPRIHSIFE 
SGYCAVCHT PYNSLEEHI QSKRHLKLI GDDGNFISI 
KGFDTFLAL DGIDAIEVN EKTRDYSPP RRRSRMPRT 
RTSSVMCDR IKDSPMSPV GSDSGHHLR SRKNINYMT 
PPLEEDSLT EKPEVVKEY RELRSSSRL LAKLADQMV 
ENDEVWNSG RPKRSCNRT RISADERLV VDNKSYYKV 
EVLSSKLRS GVTHFQEVK RPETPVEKE DDKGLIVKF 
RKLRSSELV RLNNEAENF LFPKKDETS DDDDDDDKT 
KNDNSSVSE TDDSKVKIE DDESMHSTS SESKKKRRR 
THAEAFIMD NQKYYKFET PGSRLRYHG SYLSPVPMK 
SKNNGDCVV KNESEESEP DKNFNLRLD DYNFSFESV 
PKNEAWYQT FQRRDKGEE KYVFLSDDN YWDPLILPY 
QLDYVPPLD PQECVSTYN QIKKCLMEP PSASTSVTC 
STPDPNASN SSVETPFNQ DEDSKISAT EISSNSTLD 
EIKPSRNKR SRRSGMVVN VTGTGKNPR KSPRQHAST 
LAILSSLIQ QRKRKSRRT DLDFRSNLP TILEEPKPT 
KSPKKSKVD YFSMMKRVE EEFASACDD NLDVEIEKD 
EDIAFKGQV GVEDILNVY EDNKDTIVK SPKRLVSAK 
PGRKPGKRK KNRTGWPLN RSRRMRRKE DDDSTVDSL 
SLNADSDGC DDTTDSIKK KSNCSENNN QNDQSSLSD 
VNIGDSNCD LSNADRVKN ENRSDDANS VLSNKVTSD 
GFNIDLQYQ PYVRVQKLD KNHIDNKSE PAKRSNSPK 
RTNRGQRRM PASPKSPRM LRRPRGRWY RER
    CDS sequence
    • >TC011991 RA
      atgtttgaa aagaaaacc aggttgcat gttaaaagt 
ttgttggaa aaacagttt gacaatttt cgcgaattg 
gaacagtcc gtaaaaaat catttaata gacacttcg 
ttaggtatt aaacaaact gaaaaagtt tcctcaaat 
cgctctaca aatgagcgt aacatgaca cgtaagact 
agaacaaga cggccacgc atccattct atattcgag 
tctggttat tgtgctgtt tgtcacaca ccctataac 
agccttgag gaacacatc cagtccaaa agacattta 
aaactcatc ggcgacgat ggcaatttc atatcaata 
aaaggattc gatacgttt ctggccttg gatggaatt 
gacgccatc gaagtgaat gaaaaaact cgcgactat 
tctccgccc cgccgccgc tccaggatg cctcgaacg 
cgcacctcg tccgtgatg tgcgaccga ataaaagac 
tctccgatg tcccccgtg ggcagcgac tcaggacac 
catttacgt tcaagaaaa aacataaat tatatgacg 
cctcctctc gaagaagac agtctaact gagaaaccc 
gaagtcgtg aaggaatat cgcgaatta agatccagt 
tcccgtctt ttggccaaa ctcgccgat caaatggtc 
gaaaatgac gaagtgtgg aattccggt cggccgaag 
cgatcctgc aaccgaacg aggatttcg gccgacgaa 
cgattagtc gttgataat aaaagttat tacaaagtc 
gaagttttg tcgtcgaaa cttcgttca ggagttact 
cattttcaa gaagttaaa cggccggaa acaccggtc 
gagaaagaa gatgataaa ggattgata gtaaagttt 
aggaagttg agaagttca gaactcgtc cgattgaat 
aacgaagcg gagaatttt ctgtttccg aaaaaggac 
gaaacgagt gacgacgac gacgatgat gataagacg 
aagaatgac aatagttcg gtcagcgaa accgatgat 
agtaaagtt aaaattgaa gatgacgag tcgatgcac 
agtacgagt tccgaaagc aaaaagaag aggaggcga 
acgcacgcc gaggcgttt ataatggat aatcaaaaa 
tactacaag tttgaaact cccggttca aggttgagg 
taccacggt tcatatctt tctcctgtt cctatgaaa 
tcgaaaaat aatggagat tgcgtggtg aaaaacgaa 
tcggaagag tccgaacct gataaaaac tttaacctg 
agattagat gattacaac ttttcgttt gagagcgtc 
cccaaaaac gaggcctgg tatcaaacg tttcagagg 
cgcgacaag ggggaagag aaatacgtt tttttaagt 
gacgacaat tattgggat ccgttgatt cttccctac 
caactggat tacgtaccg cctctggac ccgcaagag 
tgtgtctcg acctacaac cagatcaaa aaatgcctt 
atggagcct ccgtcagcg tcaacttcg gtcacatgc 
agcaccccc gatcccaac gcaagtaat tcaagcgtt 
gaaactccc ttcaatcaa gacgaagat agtaaaata 
agtgccact gaaattagt tcaaatagt actttggat 
gagattaaa ccgtctcga aataagcga agccgacgc 
tcgggaatg gtcgtgaat gtgaccgga actggcaaa 
aatccccgg aaatcgcct cgacaacat gcctccact 
ctcgcgatt ttgagtagt ttgatacag cagcggaaa 
cgaaagtcg cggcgaact gatcttgac ttccggtct 
aacctacca acgattttg gaggaacca aaaccgacg 
aaatcgccg aagaagtcc aaagttgat tacttttcg 
atgatgaag agggtcgag gaggagttt gcgagtgcg 
tgcgacgat aatttggat gtggagatc gaaaaggac 
gaagacatc gcgttcaag ggccaggtg ggagtggag 
gatattttg aacgtatac gaggataac aaagacact 
attgttaag tcgcctaag aggcttgta agtgcgaaa 
ccgggccgg aagccaggc aagaggaaa aaaaatcgt 
acagggtgg cctttaaac aggagcagg agaatgagg 
aggaaggag gatgatgat agcacggtg gattcgttg 
agcttgaat gcggattcg gacggatgt gacgatacg 
actgatagt attaagaag aagtcgaac tgtagtgaa 
aacaataac caaaatgac caaagtagt ttaagtgat 
gtaaatata ggcgatagt aattgtgat ttaagtaac 
gccgacagg gttaaaaac gaaaacaga agtgatgat 
gcgaactca gtactatca aataaagtg actagtgac 
ggttttaat attgatttg caatatcag ccgtatgtg 
cgcgtgcaa aaacttgat aagaatcac atagacaat 
aaaagcgaa ccggcaaag aggagtaat tcaccgaag 
cgaacaaac cggggacag aggaggatg ccggcgtcg 
ccgaaatca ccgagaatg ttgagaaga ccgagaggg 
cgctggtat cgagagaga taa
    mRNA sequence
    • >TC011991 RA
      cgaaacttc attgtggcg gattcccaa atgttttga 
aattgaggt aattcgcgt ttttagctt gtgtctttt 
gaacaaagt gtgtacgtg tgcattggg gagtttgaa 
gcgtccgtg aagccgcaa agcgtttaa aatgaggga 
aaagtcttg actgattct cggaaaagt ccagtaaac 
accattgaa gttcgaatg ggcaacgta atacagtat 
gtaactctt caagcaatg tcaaccata aacttacct 
gtggaattt ttttagatt atttttggt gatgccatg 
tttgaaaag aaaaccagg ttgcatgtt aaaagtttg 
ttggaaaaa cagtttgac aattttcgc gaattggaa 
cagtccgta aaaaatcat ttaatagac acttcgtta 
ggtattaaa caaactgaa aaagtttcc tcaaatcgc 
tctacaaat gagcgtaac atgacacgt aagactaga 
acaagacgg ccacgcatc cattctata ttcgagtct 
ggttattgt gctgtttgt cacacaccc tataacagc 
cttgaggaa cacatccag tccaaaaga catttaaaa 
ctcatcggc gacgatggc aatttcata tcaataaaa 
ggattcgat acgtttctg gccttggat ggaattgac 
gccatcgaa gtgaatgaa aaaactcgc gactattct 
ccgccccgc cgccgctcc aggatgcct cgaacgcgc 
acctcgtcc gtgatgtgc gaccgaata aaagactct 
ccgatgtcc cccgtgggc agcgactca ggacaccat 
ttacgttca agaaaaaac ataaattat atgacgcct 
cctctcgaa gaagacagt ctaactgag aaacccgaa 
gtcgtgaag gaatatcgc gaattaaga tccagttcc 
cgtcttttg gccaaactc gccgatcaa atggtcgaa 
aatgacgaa gtgtggaat tccggtcgg ccgaagcga 
tcctgcaac cgaacgagg atttcggcc gacgaacga 
ttagtcgtt gataataaa agttattac aaagtcgaa 
gttttgtcg tcgaaactt cgttcagga gttactcat 
tttcaagaa gttaaacgg ccggaaaca ccggtcgag 
aaagaagat gataaagga ttgatagta aagtttagg 
aagttgaga agttcagaa ctcgtccga ttgaataac 
gaagcggag aattttctg tttccgaaa aaggacgaa 
acgagtgac gacgacgac gatgatgat aagacgaag 
aatgacaat agttcggtc agcgaaacc gatgatagt 
aaagttaaa attgaagat gacgagtcg atgcacagt 
acgagttcc gaaagcaaa aagaagagg aggcgaacg 
cacgccgag gcgtttata atggataat caaaaatac 
tacaagttt gaaactccc ggttcaagg ttgaggtac 
cacggttca tatctttct cctgttcct atgaaatcg 
aaaaataat ggagattgc gtggtgaaa aacgaatcg 
gaagagtcc gaacctgat aaaaacttt aacctgaga 
ttagatgat tacaacttt tcgtttgag agcgtcccc 
aaaaacgag gcctggtat caaacgttt cagaggcgc 
gacaagggg gaagagaaa tacgttttt ttaagtgac 
gacaattat tgggatccg ttgattctt ccctaccaa 
ctggattac gtaccgcct ctggacccg caagagtgt 
gtctcgacc tacaaccag atcaaaaaa tgccttatg 
gagcctccg tcagcgtca acttcggtc acatgcagc 
acccccgat cccaacgca agtaattca agcgttgaa 
actcccttc aatcaagac gaagatagt aaaataagt 
gccactgaa attagttca aatagtact ttggatgag 
attaaaccg tctcgaaat aagcgaagc cgacgctcg 
ggaatggtc gtgaatgtg accggaact ggcaaaaat 
ccccggaaa tcgcctcga caacatgcc tccactctc 
gcgattttg agtagtttg atacagcag cggaaacga 
aagtcgcgg cgaactgat cttgacttc cggtctaac 
ctaccaacg attttggag gaaccaaaa ccgacgaaa 
tcgccgaag aagtccaaa gttgattac ttttcgatg 
atgaagagg gtcgaggag gagtttgcg agtgcgtgc 
gacgataat ttggatgtg gagatcgaa aaggacgaa 
gacatcgcg ttcaagggc caggtggga gtggaggat 
attttgaac gtatacgag gataacaaa gacactatt 
gttaagtcg cctaagagg cttgtaagt gcgaaaccg 
ggccggaag ccaggcaag aggaaaaaa aatcgtaca 
gggtggcct ttaaacagg agcaggaga atgaggagg 
aaggaggat gatgatagc acggtggat tcgttgagc 
ttgaatgcg gattcggac ggatgtgac gatacgact 
gatagtatt aagaagaag tcgaactgt agtgaaaac 
aataaccaa aatgaccaa agtagttta agtgatgta 
aatataggc gatagtaat tgtgattta agtaacgcc 
gacagggtt aaaaacgaa aacagaagt gatgatgcg 
aactcagta ctatcaaat aaagtgact agtgacggt 
tttaatatt gatttgcaa tatcagccg tatgtgcgc 
gtgcaaaaa cttgataag aatcacata gacaataaa 
agcgaaccg gcaaagagg agtaattca ccgaagcga 
acaaaccgg ggacagagg aggatgccg gcgtcgccg 
aaatcaccg agaatgttg agaagaccg agagggcgc 
tggtatcga gagagataa ttagatttc catgttatt 
tattttctt gtattcttt aagttaaac atgtattcg 
aatattttg taccaaatc tgtattatt ttaaaactg 
aagaatcga ttatttttt tctaatgtt taataatta 
tagtttttg catcttgga tttgttttt tttgtatat 
gtaaacata catttacct accatagaa gcatgaatt 
tttgcgcca gtactgtac cgagtacta catgtaatt 
tcgttggga aagaaaata ttacatttc aagacaaat 
tttaaataa aattgttgt ttacaa
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