| General information | |||
| TC011991 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 9144649..9150159 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC011991 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01913 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 4
- >TC011991 RA
MFEKKTRLH VKSLLEKQF DNFRELEQS VKNHLIDTS LGIKQTEKV SSNRSTNER NMTRKTRTR RPRIHSIFE SGYCAVCHT PYNSLEEHI QSKRHLKLI GDDGNFISI KGFDTFLAL DGIDAIEVN EKTRDYSPP RRRSRMPRT RTSSVMCDR IKDSPMSPV GSDSGHHLR SRKNINYMT PPLEEDSLT EKPEVVKEY RELRSSSRL LAKLADQMV ENDEVWNSG RPKRSCNRT RISADERLV VDNKSYYKV EVLSSKLRS GVTHFQEVK RPETPVEKE DDKGLIVKF RKLRSSELV RLNNEAENF LFPKKDETS DDDDDDDKT KNDNSSVSE TDDSKVKIE DDESMHSTS SESKKKRRR THAEAFIMD NQKYYKFET PGSRLRYHG SYLSPVPMK SKNNGDCVV KNESEESEP DKNFNLRLD DYNFSFESV PKNEAWYQT FQRRDKGEE KYVFLSDDN YWDPLILPY QLDYVPPLD PQECVSTYN QIKKCLMEP PSASTSVTC STPDPNASN SSVETPFNQ DEDSKISAT EISSNSTLD EIKPSRNKR SRRSGMVVN VTGTGKNPR KSPRQHAST LAILSSLIQ QRKRKSRRT DLDFRSNLP TILEEPKPT KSPKKSKVD YFSMMKRVE EEFASACDD NLDVEIEKD EDIAFKGQV GVEDILNVY EDNKDTIVK SPKRLVSAK PGRKPGKRK KNRTGWPLN RSRRMRRKE DDDSTVDSL SLNADSDGC DDTTDSIKK KSNCSENNN QNDQSSLSD VNIGDSNCD LSNADRVKN ENRSDDANS VLSNKVTSD GFNIDLQYQ PYVRVQKLD KNHIDNKSE PAKRSNSPK RTNRGQRRM PASPKSPRM LRRPRGRWY RER
- >TC011991 RA
atgtttgaa aagaaaacc aggttgcat gttaaaagt ttgttggaa aaacagttt gacaatttt cgcgaattg gaacagtcc gtaaaaaat catttaata gacacttcg ttaggtatt aaacaaact gaaaaagtt tcctcaaat cgctctaca aatgagcgt aacatgaca cgtaagact agaacaaga cggccacgc atccattct atattcgag tctggttat tgtgctgtt tgtcacaca ccctataac agccttgag gaacacatc cagtccaaa agacattta aaactcatc ggcgacgat ggcaatttc atatcaata aaaggattc gatacgttt ctggccttg gatggaatt gacgccatc gaagtgaat gaaaaaact cgcgactat tctccgccc cgccgccgc tccaggatg cctcgaacg cgcacctcg tccgtgatg tgcgaccga ataaaagac tctccgatg tcccccgtg ggcagcgac tcaggacac catttacgt tcaagaaaa aacataaat tatatgacg cctcctctc gaagaagac agtctaact gagaaaccc gaagtcgtg aaggaatat cgcgaatta agatccagt tcccgtctt ttggccaaa ctcgccgat caaatggtc gaaaatgac gaagtgtgg aattccggt cggccgaag cgatcctgc aaccgaacg aggatttcg gccgacgaa cgattagtc gttgataat aaaagttat tacaaagtc gaagttttg tcgtcgaaa cttcgttca ggagttact cattttcaa gaagttaaa cggccggaa acaccggtc gagaaagaa gatgataaa ggattgata gtaaagttt aggaagttg agaagttca gaactcgtc cgattgaat aacgaagcg gagaatttt ctgtttccg aaaaaggac gaaacgagt gacgacgac gacgatgat gataagacg aagaatgac aatagttcg gtcagcgaa accgatgat agtaaagtt aaaattgaa gatgacgag tcgatgcac agtacgagt tccgaaagc aaaaagaag aggaggcga acgcacgcc gaggcgttt ataatggat aatcaaaaa tactacaag tttgaaact cccggttca aggttgagg taccacggt tcatatctt tctcctgtt cctatgaaa tcgaaaaat aatggagat tgcgtggtg aaaaacgaa tcggaagag tccgaacct gataaaaac tttaacctg agattagat gattacaac ttttcgttt gagagcgtc cccaaaaac gaggcctgg tatcaaacg tttcagagg cgcgacaag ggggaagag aaatacgtt tttttaagt gacgacaat tattgggat ccgttgatt cttccctac caactggat tacgtaccg cctctggac ccgcaagag tgtgtctcg acctacaac cagatcaaa aaatgcctt atggagcct ccgtcagcg tcaacttcg gtcacatgc agcaccccc gatcccaac gcaagtaat tcaagcgtt gaaactccc ttcaatcaa gacgaagat agtaaaata agtgccact gaaattagt tcaaatagt actttggat gagattaaa ccgtctcga aataagcga agccgacgc tcgggaatg gtcgtgaat gtgaccgga actggcaaa aatccccgg aaatcgcct cgacaacat gcctccact ctcgcgatt ttgagtagt ttgatacag cagcggaaa cgaaagtcg cggcgaact gatcttgac ttccggtct aacctacca acgattttg gaggaacca aaaccgacg aaatcgccg aagaagtcc aaagttgat tacttttcg atgatgaag agggtcgag gaggagttt gcgagtgcg tgcgacgat aatttggat gtggagatc gaaaaggac gaagacatc gcgttcaag ggccaggtg ggagtggag gatattttg aacgtatac gaggataac aaagacact attgttaag tcgcctaag aggcttgta agtgcgaaa ccgggccgg aagccaggc aagaggaaa aaaaatcgt acagggtgg cctttaaac aggagcagg agaatgagg aggaaggag gatgatgat agcacggtg gattcgttg agcttgaat gcggattcg gacggatgt gacgatacg actgatagt attaagaag aagtcgaac tgtagtgaa aacaataac caaaatgac caaagtagt ttaagtgat gtaaatata ggcgatagt aattgtgat ttaagtaac gccgacagg gttaaaaac gaaaacaga agtgatgat gcgaactca gtactatca aataaagtg actagtgac ggttttaat attgatttg caatatcag ccgtatgtg cgcgtgcaa aaacttgat aagaatcac atagacaat aaaagcgaa ccggcaaag aggagtaat tcaccgaag cgaacaaac cggggacag aggaggatg ccggcgtcg ccgaaatca ccgagaatg ttgagaaga ccgagaggg cgctggtat cgagagaga taa
- >TC011991 RA
cgaaacttc attgtggcg gattcccaa atgttttga aattgaggt aattcgcgt ttttagctt gtgtctttt gaacaaagt gtgtacgtg tgcattggg gagtttgaa gcgtccgtg aagccgcaa agcgtttaa aatgaggga aaagtcttg actgattct cggaaaagt ccagtaaac accattgaa gttcgaatg ggcaacgta atacagtat gtaactctt caagcaatg tcaaccata aacttacct gtggaattt ttttagatt atttttggt gatgccatg tttgaaaag aaaaccagg ttgcatgtt aaaagtttg ttggaaaaa cagtttgac aattttcgc gaattggaa cagtccgta aaaaatcat ttaatagac acttcgtta ggtattaaa caaactgaa aaagtttcc tcaaatcgc tctacaaat gagcgtaac atgacacgt aagactaga acaagacgg ccacgcatc cattctata ttcgagtct ggttattgt gctgtttgt cacacaccc tataacagc cttgaggaa cacatccag tccaaaaga catttaaaa ctcatcggc gacgatggc aatttcata tcaataaaa ggattcgat acgtttctg gccttggat ggaattgac gccatcgaa gtgaatgaa aaaactcgc gactattct ccgccccgc cgccgctcc aggatgcct cgaacgcgc acctcgtcc gtgatgtgc gaccgaata aaagactct ccgatgtcc cccgtgggc agcgactca ggacaccat ttacgttca agaaaaaac ataaattat atgacgcct cctctcgaa gaagacagt ctaactgag aaacccgaa gtcgtgaag gaatatcgc gaattaaga tccagttcc cgtcttttg gccaaactc gccgatcaa atggtcgaa aatgacgaa gtgtggaat tccggtcgg ccgaagcga tcctgcaac cgaacgagg atttcggcc gacgaacga ttagtcgtt gataataaa agttattac aaagtcgaa gttttgtcg tcgaaactt cgttcagga gttactcat tttcaagaa gttaaacgg ccggaaaca ccggtcgag aaagaagat gataaagga ttgatagta aagtttagg aagttgaga agttcagaa ctcgtccga ttgaataac gaagcggag aattttctg tttccgaaa aaggacgaa acgagtgac gacgacgac gatgatgat aagacgaag aatgacaat agttcggtc agcgaaacc gatgatagt aaagttaaa attgaagat gacgagtcg atgcacagt acgagttcc gaaagcaaa aagaagagg aggcgaacg cacgccgag gcgtttata atggataat caaaaatac tacaagttt gaaactccc ggttcaagg ttgaggtac cacggttca tatctttct cctgttcct atgaaatcg aaaaataat ggagattgc gtggtgaaa aacgaatcg gaagagtcc gaacctgat aaaaacttt aacctgaga ttagatgat tacaacttt tcgtttgag agcgtcccc aaaaacgag gcctggtat caaacgttt cagaggcgc gacaagggg gaagagaaa tacgttttt ttaagtgac gacaattat tgggatccg ttgattctt ccctaccaa ctggattac gtaccgcct ctggacccg caagagtgt gtctcgacc tacaaccag atcaaaaaa tgccttatg gagcctccg tcagcgtca acttcggtc acatgcagc acccccgat cccaacgca agtaattca agcgttgaa actcccttc aatcaagac gaagatagt aaaataagt gccactgaa attagttca aatagtact ttggatgag attaaaccg tctcgaaat aagcgaagc cgacgctcg ggaatggtc gtgaatgtg accggaact ggcaaaaat ccccggaaa tcgcctcga caacatgcc tccactctc gcgattttg agtagtttg atacagcag cggaaacga aagtcgcgg cgaactgat cttgacttc cggtctaac ctaccaacg attttggag gaaccaaaa ccgacgaaa tcgccgaag aagtccaaa gttgattac ttttcgatg atgaagagg gtcgaggag gagtttgcg agtgcgtgc gacgataat ttggatgtg gagatcgaa aaggacgaa gacatcgcg ttcaagggc caggtggga gtggaggat attttgaac gtatacgag gataacaaa gacactatt gttaagtcg cctaagagg cttgtaagt gcgaaaccg ggccggaag ccaggcaag aggaaaaaa aatcgtaca gggtggcct ttaaacagg agcaggaga atgaggagg aaggaggat gatgatagc acggtggat tcgttgagc ttgaatgcg gattcggac ggatgtgac gatacgact gatagtatt aagaagaag tcgaactgt agtgaaaac aataaccaa aatgaccaa agtagttta agtgatgta aatataggc gatagtaat tgtgattta agtaacgcc gacagggtt aaaaacgaa aacagaagt gatgatgcg aactcagta ctatcaaat aaagtgact agtgacggt tttaatatt gatttgcaa tatcagccg tatgtgcgc gtgcaaaaa cttgataag aatcacata gacaataaa agcgaaccg gcaaagagg agtaattca ccgaagcga acaaaccgg ggacagagg aggatgccg gcgtcgccg aaatcaccg agaatgttg agaagaccg agagggcgc tggtatcga gagagataa ttagatttc catgttatt tattttctt gtattcttt aagttaaac atgtattcg aatattttg taccaaatc tgtattatt ttaaaactg aagaatcga ttatttttt tctaatgtt taataatta tagtttttg catcttgga tttgttttt tttgtatat gtaaacata catttacct accatagaa gcatgaatt tttgcgcca gtactgtac cgagtacta catgtaatt tcgttggga aagaaaata ttacatttc aagacaaat tttaaataa aattgttgt ttacaa
