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Gene information
General information
TC011980 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9372985..9377752
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC011980
    iBeetle screen iB_01911

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011980 RA
      MGDRSYADY SQPPPSTAY PNTYGAPPT GGFNPPGGG 
GGGAGGGGG SWNQGRSSG GGYGGGQGG GGGYNQGGG 
YNSGGGGGY GNSGGYGGG GGGGYGDNG GGSNLVVQV 
DTIFVSGMN PNLTEDEIA QHFGSIGVI KMDKKTQKR 
KIWLYKDKQ TGLSKGEAT VTYDDSNAA QSAISWFDG 
KEFGGTQIR VQLATKKDN WSGGGGRGG GRGGGRGGG 
FGGGPRGRD GGFGGGGGG GSGGGGGGG GRDRESREG 
DWKCPNPDC GNTNFAWRN QCNRCSEDK PEGAGSGGE 
DRRGGGGGG GRDRDRGRD RRGGGGGFR GGDRGGRGF 
GGGRGGRGG FGGGRGGGG RDRGGGRDG GRSRPY
    • >TC011980 RB
      MGDRSYADY SQPPPSTAY PNTYGAPPT GGFNPPGGG 
GGGAGGGGG SWNQGRSSG GGYGGGQGG GGGYNQGGG 
YNSGGGGGY GNSGGYGGG GGGGYGDNG GGSNLVVQV 
DTIFVSGMN PNLTEDEIA QHFGSIGVI KMDKKTQKR 
KIWLYKDKQ TGLSKGEAT VTYDDSNAA QSAISWFDG 
KEFGGTQIR VQLATKKDN WSGGGGRGG GRGGGRGGG 
FGGGPRGRD GGFGGGGGG GSGGGGGGG GRDRESREG 
DWKCPNPDC GNTNFAWRN QCNRCSEDK PEGAGSGGE 
DRRGGGGGG GRDRDRGRD RRGGGGGFR GGDRGGRGF 
GGGRGGRGG FGGGRGGGG RYL
    CDS sequence
    • >TC011980 RA
      atgggcgat cgatcgtac gccgactat tcccaaccg 
ccccccagt actgcgtat cccaatacg tacggggca 
ccgccgacc ggcggtttc aaccccccg ggcggggga 
ggtggaggt gccggcggc ggcggcgga tcttggaat 
caaggacgc tcgtctggc ggcggctat ggcggcggt 
caaggtggc ggcggcggt tataaccaa ggaggtggt 
tacaactct ggcggaggc ggtggctac ggcaatagt 
ggcggttat ggcggcggc ggtggtggc ggctatgga 
gataatggc ggcggcagt aacttggtg gttcaagtc 
gacacgatt tttgtgtct ggaatgaat ccaaatctc 
accgaagac gaaattgct caacatttt ggttcgatt 
ggcgtgatt aaaatggac aagaaaacc cagaaacgg 
aagatttgg ttgtataaa gataaacag accggactt 
tcaaagggt gaagccact gttacgtat gacgattcc 
aatgcagct caatcagca atttcttgg ttcgacggg 
aaagaattc ggtggtact caaataagg gtacagttg 
gccacgaaa aaggataat tggagtggc ggtggcggc 
cgcggaggt ggaagaggt ggcggccgc gggggcggt 
tttggagga ggcccaaga ggccgagat ggaggtttc 
ggcggtggt ggtggaggc ggtagcggc ggcggcggt 
ggtggcggt ggccgagac cgcgaaagc cgcgaagga 
gactggaaa tgtccaaac ccagactgt ggcaacaca 
aattttgct tggaggaat cagtgcaat agatgcagt 
gaagataag ccggaaggt gccggttcc gggggtgaa 
gatcgcaga ggaggcggc ggcggaggc ggtagagac 
cgtgaccgt ggaagagat agaagaggt ggtggcggc 
ggtttccgc ggtggtgat cgaggaggt cgcggattt 
ggcggtgga agaggaggc cgaggcggt ttcggcggc 
ggtcgagga ggaggcgga agggatcgt ggcggcggt 
cgcgatgga ggacgttcc aggccgtat tag
    • >TC011980 RB
      atgggcgat cgatcgtac gccgactat tcccaaccg 
ccccccagt actgcgtat cccaatacg tacggggca 
ccgccgacc ggcggtttc aaccccccg ggcggggga 
ggtggaggt gccggcggc ggcggcgga tcttggaat 
caaggacgc tcgtctggc ggcggctat ggcggcggt 
caaggtggc ggcggcggt tataaccaa ggaggtggt 
tacaactct ggcggaggc ggtggctac ggcaatagt 
ggcggttat ggcggcggc ggtggtggc ggctatgga 
gataatggc ggcggcagt aacttggtg gttcaagtc 
gacacgatt tttgtgtct ggaatgaat ccaaatctc 
accgaagac gaaattgct caacatttt ggttcgatt 
ggcgtgatt aaaatggac aagaaaacc cagaaacgg 
aagatttgg ttgtataaa gataaacag accggactt 
tcaaagggt gaagccact gttacgtat gacgattcc 
aatgcagct caatcagca atttcttgg ttcgacggg 
aaagaattc ggtggtact caaataagg gtacagttg 
gccacgaaa aaggataat tggagtggc ggtggcggc 
cgcggaggt ggaagaggt ggcggccgc gggggcggt 
tttggagga ggcccaaga ggccgagat ggaggtttc 
ggcggtggt ggtggaggc ggtagcggc ggcggcggt 
ggtggcggt ggccgagac cgcgaaagc cgcgaagga 
gactggaaa tgtccaaac ccagactgt ggcaacaca 
aattttgct tggaggaat cagtgcaat agatgcagt 
gaagataag ccggaaggt gccggttcc gggggtgaa 
gatcgcaga ggaggcggc ggcggaggc ggtagagac 
cgtgaccgt ggaagagat agaagaggt ggtggcggc 
ggtttccgc ggtggtgat cgaggaggt cgcggattt 
ggcggtgga agaggaggc cgaggcggt ttcggcggc 
ggtcgagga ggaggcgga aggtatttg tga
    mRNA sequence
    • >TC011980 RA
      aggttttca gtgctgcca caacaagtt ctccagctg 
tgtttcata caaacttat tattacaat ttatatcca 
tcacaaatg ttgttttta agtacctcg acggtattc 
aagtggaac tgagaatat aaaacaagg gctgttagc 
gccgaccaa tcacaagcc cccaatccg tagcgtctt 
ttccctcgc atccatttt cctcctgtt tgttcgatt 
atcccaacg gtagaagat gggcgatcg atcgtacgc 
cgactattc ccaaccgcc ccccagtac tgcgtatcc 
caatacgta cggggcacc gccgaccgg cggtttcaa 
ccccccggg cgggggagg tggaggtgc cggcggcgg 
cggcggatc ttggaatca aggacgctc gtctggcgg 
cggctatgg cggcggtca aggtggcgg cggcggtta 
taaccaagg aggtggtta caactctgg cggaggcgg 
tggctacgg caatagtgg cggttatgg cggcggcgg 
tggtggcgg ctatggaga taatggcgg cggcagtaa 
cttggtggt tcaagtcga cacgatttt tgtgtctgg 
aatgaatcc aaatctcac cgaagacga aattgctca 
acattttgg ttcgattgg cgtgattaa aatggacaa 
gaaaaccca gaaacggaa gatttggtt gtataaaga 
taaacagac cggactttc aaagggtga agccactgt 
tacgtatga cgattccaa tgcagctca atcagcaat 
ttcttggtt cgacgggaa agaattcgg tggtactca 
aataagggt acagttggc cacgaaaaa ggataattg 
gagtggcgg tggcggccg cggaggtgg aagaggtgg 
cggccgcgg gggcggttt tggaggagg cccaagagg 
ccgagatgg aggtttcgg cggtggtgg tggaggcgg 
tagcggcgg cggcggtgg tggcggtgg ccgagaccg 
cgaaagccg cgaaggaga ctggaaatg tccaaaccc 
agactgtgg caacacaaa ttttgcttg gaggaatca 
gtgcaatag atgcagtga agataagcc ggaaggtgc 
cggttccgg gggtgaaga tcgcagagg aggcggcgg 
cggaggcgg tagagaccg tgaccgtgg aagagatag 
aagaggtgg tggcggcgg tttccgcgg tggtgatcg 
aggaggtcg cggatttgg cggtggaag aggaggccg 
aggcggttt cggcggcgg tcgaggagg aggcggaag 
ggatcgtgg cggcggtcg cgatggagg acgttccag 
gccgtatta gaagatttt ttccaaata gattcatga 
gtgttttat ttccattac agtatcata ttcatttca 
gtttttata aggtgatct ataagattt aaagctttg 
tgttttcta gtaattaca caacacgag aagttgagt 
ggtcacgag tatggcatg tttagtgaa taattattt 
tatgtataa tacattttt aagtacaaa
    • >TC011980 RB
      aggttttca gtgctgcca caacaagtt ctccagctg 
tgtttcata caaacttat tattacaat ttatatcca 
tcacaaatg ttgttttta agtacctcg acggtattc 
aagtggaac tgagaatat aaaacaagg gctgttagc 
gccgaccaa tcacaagcc cccaatccg tagcgtctt 
ttccctcgc atccatttt cctcctgtt tgttcgatt 
atcccaacg gtagaagat gggcgatcg atcgtacgc 
cgactattc ccaaccgcc ccccagtac tgcgtatcc 
caatacgta cggggcacc gccgaccgg cggtttcaa 
ccccccggg cgggggagg tggaggtgc cggcggcgg 
cggcggatc ttggaatca aggacgctc gtctggcgg 
cggctatgg cggcggtca aggtggcgg cggcggtta 
taaccaagg aggtggtta caactctgg cggaggcgg 
tggctacgg caatagtgg cggttatgg cggcggcgg 
tggtggcgg ctatggaga taatggcgg cggcagtaa 
cttggtggt tcaagtcga cacgatttt tgtgtctgg 
aatgaatcc aaatctcac cgaagacga aattgctca 
acattttgg ttcgattgg cgtgattaa aatggacaa 
gaaaaccca gaaacggaa gatttggtt gtataaaga 
taaacagac cggactttc aaagggtga agccactgt 
tacgtatga cgattccaa tgcagctca atcagcaat 
ttcttggtt cgacgggaa agaattcgg tggtactca 
aataagggt acagttggc cacgaaaaa ggataattg 
gagtggcgg tggcggccg cggaggtgg aagaggtgg 
cggccgcgg gggcggttt tggaggagg cccaagagg 
ccgagatgg aggtttcgg cggtggtgg tggaggcgg 
tagcggcgg cggcggtgg tggcggtgg ccgagaccg 
cgaaagccg cgaaggaga ctggaaatg tccaaaccc 
agactgtgg caacacaaa ttttgcttg gaggaatca 
gtgcaatag atgcagtga agataagcc ggaaggtgc 
cggttccgg gggtgaaga tcgcagagg aggcggcgg 
cggaggcgg tagagaccg tgaccgtgg aagagatag 
aagaggtgg tggcggcgg tttccgcgg tggtgatcg 
aggaggtcg cggatttgg cggtggaag aggaggccg 
aggcggttt cggcggcgg tcgaggagg aggcggaag 
gtatttgtg acaaaattt ggtgtagga aagatttta 
attttgatg tttagggat cgtggcggc ggtcgcgat 
ggaggacgt tccaggccg tattagaag attttttcc 
aaatagatt catgagtgt tttatttcc attacagta 
tcatattca tttcagttt ttataaggt gatctataa 
gatttaaag ctttgtgtt ttctagtaa ttacacaac 
acgagaagt tgagtggtc acgagtatg gcatgttta 
gtgaataat tattttatg tataataca tttttaagt 
acaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY