Status
Please wait ...
Gene information
General information
TC011980 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 9372985..9377752
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011980
    iBeetle screen iB_01911

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011980 RA
      MGDRSYADY SQPPPSTAY PNTYGAPPT GGFNPPGGG 
      GGGAGGGGG SWNQGRSSG GGYGGGQGG GGGYNQGGG 
      YNSGGGGGY GNSGGYGGG GGGGYGDNG GGSNLVVQV 
      DTIFVSGMN PNLTEDEIA QHFGSIGVI KMDKKTQKR 
      KIWLYKDKQ TGLSKGEAT VTYDDSNAA QSAISWFDG 
      KEFGGTQIR VQLATKKDN WSGGGGRGG GRGGGRGGG 
      FGGGPRGRD GGFGGGGGG GSGGGGGGG GRDRESREG 
      DWKCPNPDC GNTNFAWRN QCNRCSEDK PEGAGSGGE 
      DRRGGGGGG GRDRDRGRD RRGGGGGFR GGDRGGRGF 
      GGGRGGRGG FGGGRGGGG RDRGGGRDG GRSRPY
    • >TC011980 RB
      MGDRSYADY SQPPPSTAY PNTYGAPPT GGFNPPGGG 
      GGGAGGGGG SWNQGRSSG GGYGGGQGG GGGYNQGGG 
      YNSGGGGGY GNSGGYGGG GGGGYGDNG GGSNLVVQV 
      DTIFVSGMN PNLTEDEIA QHFGSIGVI KMDKKTQKR 
      KIWLYKDKQ TGLSKGEAT VTYDDSNAA QSAISWFDG 
      KEFGGTQIR VQLATKKDN WSGGGGRGG GRGGGRGGG 
      FGGGPRGRD GGFGGGGGG GSGGGGGGG GRDRESREG 
      DWKCPNPDC GNTNFAWRN QCNRCSEDK PEGAGSGGE 
      DRRGGGGGG GRDRDRGRD RRGGGGGFR GGDRGGRGF 
      GGGRGGRGG FGGGRGGGG RYL
    CDS sequence
    • >TC011980 RA
      atgggcgat cgatcgtac gccgactat tcccaaccg 
      ccccccagt actgcgtat cccaatacg tacggggca 
      ccgccgacc ggcggtttc aaccccccg ggcggggga 
      ggtggaggt gccggcggc ggcggcgga tcttggaat 
      caaggacgc tcgtctggc ggcggctat ggcggcggt 
      caaggtggc ggcggcggt tataaccaa ggaggtggt 
      tacaactct ggcggaggc ggtggctac ggcaatagt 
      ggcggttat ggcggcggc ggtggtggc ggctatgga 
      gataatggc ggcggcagt aacttggtg gttcaagtc 
      gacacgatt tttgtgtct ggaatgaat ccaaatctc 
      accgaagac gaaattgct caacatttt ggttcgatt 
      ggcgtgatt aaaatggac aagaaaacc cagaaacgg 
      aagatttgg ttgtataaa gataaacag accggactt 
      tcaaagggt gaagccact gttacgtat gacgattcc 
      aatgcagct caatcagca atttcttgg ttcgacggg 
      aaagaattc ggtggtact caaataagg gtacagttg 
      gccacgaaa aaggataat tggagtggc ggtggcggc 
      cgcggaggt ggaagaggt ggcggccgc gggggcggt 
      tttggagga ggcccaaga ggccgagat ggaggtttc 
      ggcggtggt ggtggaggc ggtagcggc ggcggcggt 
      ggtggcggt ggccgagac cgcgaaagc cgcgaagga 
      gactggaaa tgtccaaac ccagactgt ggcaacaca 
      aattttgct tggaggaat cagtgcaat agatgcagt 
      gaagataag ccggaaggt gccggttcc gggggtgaa 
      gatcgcaga ggaggcggc ggcggaggc ggtagagac 
      cgtgaccgt ggaagagat agaagaggt ggtggcggc 
      ggtttccgc ggtggtgat cgaggaggt cgcggattt 
      ggcggtgga agaggaggc cgaggcggt ttcggcggc 
      ggtcgagga ggaggcgga agggatcgt ggcggcggt 
      cgcgatgga ggacgttcc aggccgtat tag
    • >TC011980 RB
      atgggcgat cgatcgtac gccgactat tcccaaccg 
      ccccccagt actgcgtat cccaatacg tacggggca 
      ccgccgacc ggcggtttc aaccccccg ggcggggga 
      ggtggaggt gccggcggc ggcggcgga tcttggaat 
      caaggacgc tcgtctggc ggcggctat ggcggcggt 
      caaggtggc ggcggcggt tataaccaa ggaggtggt 
      tacaactct ggcggaggc ggtggctac ggcaatagt 
      ggcggttat ggcggcggc ggtggtggc ggctatgga 
      gataatggc ggcggcagt aacttggtg gttcaagtc 
      gacacgatt tttgtgtct ggaatgaat ccaaatctc 
      accgaagac gaaattgct caacatttt ggttcgatt 
      ggcgtgatt aaaatggac aagaaaacc cagaaacgg 
      aagatttgg ttgtataaa gataaacag accggactt 
      tcaaagggt gaagccact gttacgtat gacgattcc 
      aatgcagct caatcagca atttcttgg ttcgacggg 
      aaagaattc ggtggtact caaataagg gtacagttg 
      gccacgaaa aaggataat tggagtggc ggtggcggc 
      cgcggaggt ggaagaggt ggcggccgc gggggcggt 
      tttggagga ggcccaaga ggccgagat ggaggtttc 
      ggcggtggt ggtggaggc ggtagcggc ggcggcggt 
      ggtggcggt ggccgagac cgcgaaagc cgcgaagga 
      gactggaaa tgtccaaac ccagactgt ggcaacaca 
      aattttgct tggaggaat cagtgcaat agatgcagt 
      gaagataag ccggaaggt gccggttcc gggggtgaa 
      gatcgcaga ggaggcggc ggcggaggc ggtagagac 
      cgtgaccgt ggaagagat agaagaggt ggtggcggc 
      ggtttccgc ggtggtgat cgaggaggt cgcggattt 
      ggcggtgga agaggaggc cgaggcggt ttcggcggc 
      ggtcgagga ggaggcgga aggtatttg tga
    mRNA sequence
    • >TC011980 RA
      aggttttca gtgctgcca caacaagtt ctccagctg 
      tgtttcata caaacttat tattacaat ttatatcca 
      tcacaaatg ttgttttta agtacctcg acggtattc 
      aagtggaac tgagaatat aaaacaagg gctgttagc 
      gccgaccaa tcacaagcc cccaatccg tagcgtctt 
      ttccctcgc atccatttt cctcctgtt tgttcgatt 
      atcccaacg gtagaagat gggcgatcg atcgtacgc 
      cgactattc ccaaccgcc ccccagtac tgcgtatcc 
      caatacgta cggggcacc gccgaccgg cggtttcaa 
      ccccccggg cgggggagg tggaggtgc cggcggcgg 
      cggcggatc ttggaatca aggacgctc gtctggcgg 
      cggctatgg cggcggtca aggtggcgg cggcggtta 
      taaccaagg aggtggtta caactctgg cggaggcgg 
      tggctacgg caatagtgg cggttatgg cggcggcgg 
      tggtggcgg ctatggaga taatggcgg cggcagtaa 
      cttggtggt tcaagtcga cacgatttt tgtgtctgg 
      aatgaatcc aaatctcac cgaagacga aattgctca 
      acattttgg ttcgattgg cgtgattaa aatggacaa 
      gaaaaccca gaaacggaa gatttggtt gtataaaga 
      taaacagac cggactttc aaagggtga agccactgt 
      tacgtatga cgattccaa tgcagctca atcagcaat 
      ttcttggtt cgacgggaa agaattcgg tggtactca 
      aataagggt acagttggc cacgaaaaa ggataattg 
      gagtggcgg tggcggccg cggaggtgg aagaggtgg 
      cggccgcgg gggcggttt tggaggagg cccaagagg 
      ccgagatgg aggtttcgg cggtggtgg tggaggcgg 
      tagcggcgg cggcggtgg tggcggtgg ccgagaccg 
      cgaaagccg cgaaggaga ctggaaatg tccaaaccc 
      agactgtgg caacacaaa ttttgcttg gaggaatca 
      gtgcaatag atgcagtga agataagcc ggaaggtgc 
      cggttccgg gggtgaaga tcgcagagg aggcggcgg 
      cggaggcgg tagagaccg tgaccgtgg aagagatag 
      aagaggtgg tggcggcgg tttccgcgg tggtgatcg 
      aggaggtcg cggatttgg cggtggaag aggaggccg 
      aggcggttt cggcggcgg tcgaggagg aggcggaag 
      ggatcgtgg cggcggtcg cgatggagg acgttccag 
      gccgtatta gaagatttt ttccaaata gattcatga 
      gtgttttat ttccattac agtatcata ttcatttca 
      gtttttata aggtgatct ataagattt aaagctttg 
      tgttttcta gtaattaca caacacgag aagttgagt 
      ggtcacgag tatggcatg tttagtgaa taattattt 
      tatgtataa tacattttt aagtacaaa
    • >TC011980 RB
      aggttttca gtgctgcca caacaagtt ctccagctg 
      tgtttcata caaacttat tattacaat ttatatcca 
      tcacaaatg ttgttttta agtacctcg acggtattc 
      aagtggaac tgagaatat aaaacaagg gctgttagc 
      gccgaccaa tcacaagcc cccaatccg tagcgtctt 
      ttccctcgc atccatttt cctcctgtt tgttcgatt 
      atcccaacg gtagaagat gggcgatcg atcgtacgc 
      cgactattc ccaaccgcc ccccagtac tgcgtatcc 
      caatacgta cggggcacc gccgaccgg cggtttcaa 
      ccccccggg cgggggagg tggaggtgc cggcggcgg 
      cggcggatc ttggaatca aggacgctc gtctggcgg 
      cggctatgg cggcggtca aggtggcgg cggcggtta 
      taaccaagg aggtggtta caactctgg cggaggcgg 
      tggctacgg caatagtgg cggttatgg cggcggcgg 
      tggtggcgg ctatggaga taatggcgg cggcagtaa 
      cttggtggt tcaagtcga cacgatttt tgtgtctgg 
      aatgaatcc aaatctcac cgaagacga aattgctca 
      acattttgg ttcgattgg cgtgattaa aatggacaa 
      gaaaaccca gaaacggaa gatttggtt gtataaaga 
      taaacagac cggactttc aaagggtga agccactgt 
      tacgtatga cgattccaa tgcagctca atcagcaat 
      ttcttggtt cgacgggaa agaattcgg tggtactca 
      aataagggt acagttggc cacgaaaaa ggataattg 
      gagtggcgg tggcggccg cggaggtgg aagaggtgg 
      cggccgcgg gggcggttt tggaggagg cccaagagg 
      ccgagatgg aggtttcgg cggtggtgg tggaggcgg 
      tagcggcgg cggcggtgg tggcggtgg ccgagaccg 
      cgaaagccg cgaaggaga ctggaaatg tccaaaccc 
      agactgtgg caacacaaa ttttgcttg gaggaatca 
      gtgcaatag atgcagtga agataagcc ggaaggtgc 
      cggttccgg gggtgaaga tcgcagagg aggcggcgg 
      cggaggcgg tagagaccg tgaccgtgg aagagatag 
      aagaggtgg tggcggcgg tttccgcgg tggtgatcg 
      aggaggtcg cggatttgg cggtggaag aggaggccg 
      aggcggttt cggcggcgg tcgaggagg aggcggaag 
      gtatttgtg acaaaattt ggtgtagga aagatttta 
      attttgatg tttagggat cgtggcggc ggtcgcgat 
      ggaggacgt tccaggccg tattagaag attttttcc 
      aaatagatt catgagtgt tttatttcc attacagta 
      tcatattca tttcagttt ttataaggt gatctataa 
      gatttaaag ctttgtgtt ttctagtaa ttacacaac 
      acgagaagt tgagtggtc acgagtatg gcatgttta 
      gtgaataat tattttatg tataataca tttttaagt 
      acaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY