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Gene information
General information
TC011969 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 2789058..2797062
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011969
    iBeetle screen iB_01910
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands slightlycolour darker - Number of animals showing the phenotype: 6 (41 dpi)
    Days post injection


    Comment: waited 1 week longer to let the phenotype develop

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011969 RA
      MREIVHLQI GQAGNNIGS KFWERINVY FNEATGGRY 
      VPRAVLVDL EAGTMDTVR SGPFGRLFA PDNFVFGRE 
      GAGNNFAKG HYTEGAELA DTVLDITRK EAEGCDCLQ 
      GFQIVHSLG GGTGSGLGC LLLGKIREE YPDRIISTF 
      SIIPSPKVS DTVVEPYNT VMSIHHLVE NTDETYIID 
      NEALHDICF RTLKLSAPT LADLNHLIS AAMSGITAC 
      IRFPGQLNA DLRKIQVNM VPFPRLHFF MPGFAPLVS 
      RGMRPYQAV TVPELVHQL FDAKNMMCA CDPRRGRYL 
      TAASIFRGR MSTKEVDEQ MYNIQNKNS SYFIEWIPN 
      NLKSAICDI PPRGLKICG TFIGNTTAI QELFKRVGE 
      QFSAMFRRK AFVHWYTGE GMEEMEFTE AESNMNDLI 
      SEYQQYQEA PAEYDEAEI EELDDEDDE EY
    CDS sequence
    • >TC011969 RA
      atgcgtgaa attgtgcat ctccagatt ggtcaagct 
      ggtaataat atcggctca aaattttgg gaacggata 
      aacgtctat ttcaatgaa gccacaggt ggcagatac 
      gtcccccgc gcggtcctc gtggacttg gaagccggg 
      acgatggat acggtccgt tcgggcccc ttcggccgc 
      ctcttcgcc cccgacaat ttcgtgttc gggcgcgaa 
      ggcgccggc aacaacttc gccaaaggg cattacacc 
      gaaggggcc gagttagcg gacactgtc ctggacatc 
      acccgcaag gaggccgaa ggctgtgac tgcctgcaa 
      ggcttccag atcgtgcac tccctcggt ggaggcacc 
      ggatcgggc ctagggtgt ctcctgttg gggaaaata 
      cgcgaggag tatcccgac aggatcatc agcacgttt 
      tcgataatc ccaagccct aaggtttca gacacggtg 
      gttgagccc tacaatact gtaatgtcg attcaccat 
      ttggtcgaa aataccgac gagacgtac attatcgac 
      aatgaggcg ttgcatgac atttgtttc aggacgctg 
      aaactttca gcgccgact ttagccgat ttgaatcat 
      ttaatctca gctgcaatg tctgggata acggcttgt 
      attcgtttt ccggggcaa ttaaatgcc gatttaaga 
      aagatccag gttaatatg gtgcccttc ccgcgtttg 
      cacttcttc atgcccgga tttgcgcct ttggtgtcg 
      cgcggaatg cggccgtat caagccgtg accgttccg 
      gagctggtg catcagctc ttcgatgcg aaaaatatg 
      atgtgtgct tgtgatccc agaagaggt agatatctg 
      acggctgcg tcgattttt cgtggccgc atgtcgacc 
      aaggaagtc gacgaacag atgtacaac atccagaat 
      aagaacagt agttatttt attgagtgg attccgaat 
      aatttgaag agtgcgata tgtgatatt ccgccgagg 
      ggcttgaag atttgtggg acgtttatt ggcaatacg 
      accgcgatt caagagttg tttaaaagg gttggggaa 
      caattttcg gctatgttt cggaggaag gcttttgtg 
      cattggtat acgggtgag gggatggaa gaaatggag 
      tttacggag gctgagtcg aatatgaat gatctgatt 
      tcggagtat caacagtat caagaagcg ccagctgag 
      tatgacgaa gcggaaatt gaggaattg gacgatgaa 
      gatgatgag gaatactaa
    mRNA sequence
    • >TC011969 RA
      aacaaggtg taaatttgg tggaggcgc cgggcaacg 
      cgctgtcaa acgtgtgac tgtatttcg aagacttgt 
      ctaaatttt tgtgttgta aaatgcgtg aaattgtgc 
      atctccaga ttggtcaag ctggtaata atatcggct 
      caaaatttt gggaacgga taaacgtct atttcaatg 
      aagccacag gtggcagat acgtccccc gcgcggtcc 
      tcgtggact tggaagccg ggacgatgg atacggtcc 
      gttcgggcc ccttcggcc gcctcttcg cccccgaca 
      atttcgtgt tcgggcgcg aaggcgccg gcaacaact 
      tcgccaaag ggcattaca ccgaagggg ccgagttag 
      cggacactg tcctggaca tcacccgca aggaggccg 
      aaggctgtg actgcctgc aaggcttcc agatcgtgc 
      actccctcg gtggaggca ccggatcgg gcctagggt 
      gtctcctgt tggggaaaa tacgcgagg agtatcccg 
      acaggatca tcagcacgt tttcgataa tcccaagcc 
      ctaaggttt cagacacgg tggttgagc cctacaata 
      ctgtaatgt cgattcacc atttggtcg aaaataccg 
      acgagacgt acattatcg acaatgagg cgttgcatg 
      acatttgtt tcaggacgc tgaaacttt cagcgccga 
      ctttagccg atttgaatc atttaatct cagctgcaa 
      tgtctggga taacggctt gtattcgtt ttccggggc 
      aattaaatg ccgatttaa gaaagatcc aggttaata 
      tggtgccct tcccgcgtt tgcacttct tcatgcccg 
      gatttgcgc ctttggtgt cgcgcggaa tgcggccgt 
      atcaagccg tgaccgttc cggagctgg tgcatcagc 
      tcttcgatg cgaaaaata tgatgtgtg cttgtgatc 
      ccagaagag gtagatatc tgacggctg cgtcgattt 
      ttcgtggcc gcatgtcga ccaaggaag tcgacgaac 
      agatgtaca acatccaga ataagaaca gtagttatt 
      ttattgagt ggattccga ataatttga agagtgcga 
      tatgtgata ttccgccga ggggcttga agatttgtg 
      ggacgttta ttggcaata cgaccgcga ttcaagagt 
      tgtttaaaa gggttgggg aacaatttt cggctatgt 
      ttcggagga aggcttttg tgcattggt atacgggtg 
      aggggatgg aagaaatgg agtttacgg aggctgagt 
      cgaatatga atgatctga tttcggagt atcaacagt 
      atcaagaag cgccagctg agtatgacg aagcggaaa 
      ttgaggaat tggacgatg aagatgatg aggaatact 
      aataatact gctattttt taaacatac taagcattg 
      aaaaataaa taaaaaatg tagacccta