Status
Please wait ...
Gene information
General information
TC011953 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11006973..11010193
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011953
    iBeetle screen iB_01908

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    Pupal morphology
    elytra blistered - Number of animals showing the phenotype: 6

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011953 RA
      MADLKSLEH PTLKVPYEI LNKKFRTAQ KSLDREASH 
      VQQAARELE STISGGNVR ARDITSLLG GMVEKLQVL 
      KRKAEESIS EELAASNVC KRRLEHLKE RETLTSAGT 
      VSQGAVNQW KRKRLDRMM VEYFLRNGY YNAAITLAE 
      KSDIKDLTN IEIFLTSRE IEKSLANHE TSKCLIWCH 
      DNKSKLRKL KSNMEFNLR IQEFVELIR SERRMDAIK 
      HARKHFPSF EDEHLDTIQ RVMALLAFP IGTEIKPYK 
      ALFDETRWD TLIEQFRQE NYRLFQLAS QSVFTVALQ 
      AGLSALKTP YPLMHFTCV FLY
    • >TC011953 RB
      MADLKSLEH PTLKVPYEI LNKKFRTAQ KSLDREASH 
      VQQAARELE STISGGNVR ARDITSLLG GMVEKLQVL 
      KRKAEESIS EELAASNVC KRRLEHLKE RETLTSAGT 
      VSQGAVNQW KRKRLDRMM VEYFLRNGY YNAAITLAE 
      KSDIKDLTN IEIFLTSRE IEKSLANHE TSKCLIWCH 
      DNKSKLRKL KSNMEFNLR IQEFVELIR SERRMDAIK 
      HARKHFPSF EDEHLDTIQ RVMALLAFP IGTEIKPYK 
      ALFDETRWD TLIEQFRQE NYRLFQLAS QSVFTVALQ 
      AGLSALKTP YPLMHFTCC YSDNGENRN PACPVCQPC 
      LNQLAESLP FAHCSQSRL YCHISGLPL NENNLPMML 
      PNGYIYGEQ ALEQMAKEN NGQIICPKT KEIFPFKKV 
      EKVYVM
    CDS sequence
    • >TC011953 RA
      atggcagac ttgaaatct ctggagcat cccacgttg 
      aaggtgccg tacgagatc ctcaacaag aagttccgg 
      actgcgcaa aaatcgctc gacagggag gcctcccac 
      gtgcagcag gcggcccgc gagctcgag agcaccatc 
      tctgggggt aatgtgagg gcgcgcgac atcacctcg 
      ctgcttgga ggcatggtg gagaagctg caggtgctg 
      aagcgcaaa gccgaggag agcatctcc gaggagctg 
      gccgcctcg aatgtgtgc aagcggcgg ctggagcac 
      ctgaaggag cgcgagacg ctcacgtcg gccgggacg 
      gtctcgcag ggggccgtc aaccagtgg aagaggaag 
      cggctggat aggatgatg gtggagtac ttcctcagg 
      aatgggtat tacaacgct gcgattact ctggcggag 
      aagagcgat atcaaggac ttgaccaat attgagatt 
      tttttgacg tcgcgcgag atcgagaag agtctcgcg 
      aatcatgag acgtccaag tgcttgatt tggtgccat 
      gacaataag tccaaactt cgtaagctt aagtcaaat 
      atggagttt aatttgagg atccaggag tttgttgaa 
      ctgatcagg tctgagcgc agaatggac gctattaag 
      cacgcgaga aagcatttt ccgagcttc gaagacgag 
      catttagat acgattcag agggttatg gcgcttttg 
      gcgtttccg ataggtaca gaaataaaa ccctacaag 
      gccctcttt gacgagacc agatgggac accctcatc 
      gagcaattt agacaagaa aactaccga ctgttccaa 
      ttagcttca caatcagtg tttacagtg gctcttcaa 
      gctggactt tcagcgctt aaaacacca tatcctttg 
      atgcatttt acgtgtgtg tttttgtac tga
    • >TC011953 RB
      atggcagac ttgaaatct ctggagcat cccacgttg 
      aaggtgccg tacgagatc ctcaacaag aagttccgg 
      actgcgcaa aaatcgctc gacagggag gcctcccac 
      gtgcagcag gcggcccgc gagctcgag agcaccatc 
      tctgggggt aatgtgagg gcgcgcgac atcacctcg 
      ctgcttgga ggcatggtg gagaagctg caggtgctg 
      aagcgcaaa gccgaggag agcatctcc gaggagctg 
      gccgcctcg aatgtgtgc aagcggcgg ctggagcac 
      ctgaaggag cgcgagacg ctcacgtcg gccgggacg 
      gtctcgcag ggggccgtc aaccagtgg aagaggaag 
      cggctggat aggatgatg gtggagtac ttcctcagg 
      aatgggtat tacaacgct gcgattact ctggcggag 
      aagagcgat atcaaggac ttgaccaat attgagatt 
      tttttgacg tcgcgcgag atcgagaag agtctcgcg 
      aatcatgag acgtccaag tgcttgatt tggtgccat 
      gacaataag tccaaactt cgtaagctt aagtcaaat 
      atggagttt aatttgagg atccaggag tttgttgaa 
      ctgatcagg tctgagcgc agaatggac gctattaag 
      cacgcgaga aagcatttt ccgagcttc gaagacgag 
      catttagat acgattcag agggttatg gcgcttttg 
      gcgtttccg ataggtaca gaaataaaa ccctacaag 
      gccctcttt gacgagacc agatgggac accctcatc 
      gagcaattt agacaagaa aactaccga ctgttccaa 
      ttagcttca caatcagtg tttacagtg gctcttcaa 
      gctggactt tcagcgctt aaaacacca tatcctttg 
      atgcatttt acgtgttgt tattctgat aacggggaa 
      aacaggaat cccgcctgt ccagtttgc caaccttgc 
      ctcaaccaa ttagcagag tctttgcct ttcgcccat 
      tgctcacag tcacgtctt tattgccat attagcgga 
      ttaccacta aacgaaaac aatttaccc atgatgttg 
      cccaatgga tatatttac ggagaacaa gcgctagaa 
      caaatggcg aaggagaat aacgggcaa ataatatgt 
      cccaaaacg aaagagata ttcccgttt aagaaagtc 
      gaaaaagtt tacgttatg taa
    mRNA sequence
    • >TC011953 RA
      actccttct ttaaaaata ccaaacttg ctaattgta 
      tgtgctttt gccgtgtac tcgcaaatt tcgcgtttc 
      ccgaacttc cctgccatt tctattgac aaaatcagc 
      tgtttgcga agccagcag ctgagtgag tgacgtttt 
      atttatgtt tttatttgt gcaccaaaa tggcagact 
      tgaaatctc tggagcatc ccacgttga aggtgccgt 
      acgagatcc tcaacaaga agttccgga ctgcgcaaa 
      aatcgctcg acagggagg cctcccacg tgcagcagg 
      cggcccgcg agctcgaga gcaccatct ctgggggta 
      atgtgaggg cgcgcgaca tcacctcgc tgcttggag 
      gcatggtgg agaagctgc aggtgctga agcgcaaag 
      ccgaggaga gcatctccg aggagctgg ccgcctcga 
      atgtgtgca agcggcggc tggagcacc tgaaggagc 
      gcgagacgc tcacgtcgg ccgggacgg tctcgcagg 
      gggccgtca accagtgga agaggaagc ggctggata 
      ggatgatgg tggagtact tcctcagga atgggtatt 
      acaacgctg cgattactc tggcggaga agagcgata 
      tcaaggact tgaccaata ttgagattt ttttgacgt 
      cgcgcgaga tcgagaaga gtctcgcga atcatgaga 
      cgtccaagt gcttgattt ggtgccatg acaataagt 
      ccaaacttc gtaagctta agtcaaata tggagttta 
      atttgagga tccaggagt ttgttgaac tgatcaggt 
      ctgagcgca gaatggacg ctattaagc acgcgagaa 
      agcattttc cgagcttcg aagacgagc atttagata 
      cgattcaga gggttatgg cgcttttgg cgtttccga 
      taggtacag aaataaaac cctacaagg ccctctttg 
      acgagacca gatgggaca ccctcatcg agcaattta 
      gacaagaaa actaccgac tgttccaat tagcttcac 
      aatcagtgt ttacagtgg ctcttcaag ctggacttt 
      cagcgctta aaacaccat atcctttga tgcatttta 
      cgtgtgtgt ttttgtact gagtgttgt tccttaaca 
      aaacttaat cagtgttat tctgataac ggggaaaac 
      aggaatccc gcctgtcca gtttgccaa ccttgcctc 
      aaccaatta gcagagtct ttgcctttc gcccattgc 
      tcacagtca cgtctttat tgccatatt agcggatta 
      ccactaaac gaaaacaat ttacccatg atgttgccc 
      aatggatat atttacgga gaacaagta agttgattt 
      tgcgattgg tcggttgtt actggacga cttgcaggc 
      gctagaaca aatggcgaa ggagaataa cgggcaaat 
      aatatgtcc caaaacgaa agagatatt cccgtttaa 
      gaaagtcga aaaagttta cgttatgta aatgtgata 
      ttttctttt ttatagtat tgtttatac ctctctaag 
      ttgagttaa gttaagtac tcatagggt aacagtttt 
      ttgtgaaat atactttat gcttatcg
    • >TC011953 RB
      actccttct ttaaaaata ccaaacttg ctaattgta 
      tgtgctttt gccgtgtac tcgcaaatt tcgcgtttc 
      ccgaacttc cctgccatt tctattgac aaaatcagc 
      tgtttgcga agccagcag ctgagtgag tgacgtttt 
      atttatgtt tttatttgt gcaccaaaa tggcagact 
      tgaaatctc tggagcatc ccacgttga aggtgccgt 
      acgagatcc tcaacaaga agttccgga ctgcgcaaa 
      aatcgctcg acagggagg cctcccacg tgcagcagg 
      cggcccgcg agctcgaga gcaccatct ctgggggta 
      atgtgaggg cgcgcgaca tcacctcgc tgcttggag 
      gcatggtgg agaagctgc aggtgctga agcgcaaag 
      ccgaggaga gcatctccg aggagctgg ccgcctcga 
      atgtgtgca agcggcggc tggagcacc tgaaggagc 
      gcgagacgc tcacgtcgg ccgggacgg tctcgcagg 
      gggccgtca accagtgga agaggaagc ggctggata 
      ggatgatgg tggagtact tcctcagga atgggtatt 
      acaacgctg cgattactc tggcggaga agagcgata 
      tcaaggact tgaccaata ttgagattt ttttgacgt 
      cgcgcgaga tcgagaaga gtctcgcga atcatgaga 
      cgtccaagt gcttgattt ggtgccatg acaataagt 
      ccaaacttc gtaagctta agtcaaata tggagttta 
      atttgagga tccaggagt ttgttgaac tgatcaggt 
      ctgagcgca gaatggacg ctattaagc acgcgagaa 
      agcattttc cgagcttcg aagacgagc atttagata 
      cgattcaga gggttatgg cgcttttgg cgtttccga 
      taggtacag aaataaaac cctacaagg ccctctttg 
      acgagacca gatgggaca ccctcatcg agcaattta 
      gacaagaaa actaccgac tgttccaat tagcttcac 
      aatcagtgt ttacagtgg ctcttcaag ctggacttt 
      cagcgctta aaacaccat atcctttga tgcatttta 
      cgtgttgtt attctgata acggggaaa acaggaatc 
      ccgcctgtc cagtttgcc aaccttgcc tcaaccaat 
      tagcagagt ctttgcctt tcgcccatt gctcacagt 
      cacgtcttt attgccata ttagcggat taccactaa 
      acgaaaaca atttaccca tgatgttgc ccaatggat 
      atatttacg gagaacaag cgctagaac aaatggcga 
      aggagaata acgggcaaa taatatgtc ccaaaacga 
      aagagatat tcccgttta agaaagtcg aaaaagttt 
      acgttatgt aaatgtgat attttcttt tttatagta 
      ttgtttata cctctctaa gttgagtta agttaagta 
      ctcataggg taacagttt tttgtgaaa tatacttta 
      tgcttatcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY