General information | |||
TC011953 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 11006973..11010193 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC011953 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01908 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
- >TC011953 RA
MADLKSLEH PTLKVPYEI LNKKFRTAQ KSLDREASH VQQAARELE STISGGNVR ARDITSLLG GMVEKLQVL KRKAEESIS EELAASNVC KRRLEHLKE RETLTSAGT VSQGAVNQW KRKRLDRMM VEYFLRNGY YNAAITLAE KSDIKDLTN IEIFLTSRE IEKSLANHE TSKCLIWCH DNKSKLRKL KSNMEFNLR IQEFVELIR SERRMDAIK HARKHFPSF EDEHLDTIQ RVMALLAFP IGTEIKPYK ALFDETRWD TLIEQFRQE NYRLFQLAS QSVFTVALQ AGLSALKTP YPLMHFTCV FLY
- >TC011953 RB
MADLKSLEH PTLKVPYEI LNKKFRTAQ KSLDREASH VQQAARELE STISGGNVR ARDITSLLG GMVEKLQVL KRKAEESIS EELAASNVC KRRLEHLKE RETLTSAGT VSQGAVNQW KRKRLDRMM VEYFLRNGY YNAAITLAE KSDIKDLTN IEIFLTSRE IEKSLANHE TSKCLIWCH DNKSKLRKL KSNMEFNLR IQEFVELIR SERRMDAIK HARKHFPSF EDEHLDTIQ RVMALLAFP IGTEIKPYK ALFDETRWD TLIEQFRQE NYRLFQLAS QSVFTVALQ AGLSALKTP YPLMHFTCC YSDNGENRN PACPVCQPC LNQLAESLP FAHCSQSRL YCHISGLPL NENNLPMML PNGYIYGEQ ALEQMAKEN NGQIICPKT KEIFPFKKV EKVYVM
- >TC011953 RA
atggcagac ttgaaatct ctggagcat cccacgttg aaggtgccg tacgagatc ctcaacaag aagttccgg actgcgcaa aaatcgctc gacagggag gcctcccac gtgcagcag gcggcccgc gagctcgag agcaccatc tctgggggt aatgtgagg gcgcgcgac atcacctcg ctgcttgga ggcatggtg gagaagctg caggtgctg aagcgcaaa gccgaggag agcatctcc gaggagctg gccgcctcg aatgtgtgc aagcggcgg ctggagcac ctgaaggag cgcgagacg ctcacgtcg gccgggacg gtctcgcag ggggccgtc aaccagtgg aagaggaag cggctggat aggatgatg gtggagtac ttcctcagg aatgggtat tacaacgct gcgattact ctggcggag aagagcgat atcaaggac ttgaccaat attgagatt tttttgacg tcgcgcgag atcgagaag agtctcgcg aatcatgag acgtccaag tgcttgatt tggtgccat gacaataag tccaaactt cgtaagctt aagtcaaat atggagttt aatttgagg atccaggag tttgttgaa ctgatcagg tctgagcgc agaatggac gctattaag cacgcgaga aagcatttt ccgagcttc gaagacgag catttagat acgattcag agggttatg gcgcttttg gcgtttccg ataggtaca gaaataaaa ccctacaag gccctcttt gacgagacc agatgggac accctcatc gagcaattt agacaagaa aactaccga ctgttccaa ttagcttca caatcagtg tttacagtg gctcttcaa gctggactt tcagcgctt aaaacacca tatcctttg atgcatttt acgtgtgtg tttttgtac tga
- >TC011953 RB
atggcagac ttgaaatct ctggagcat cccacgttg aaggtgccg tacgagatc ctcaacaag aagttccgg actgcgcaa aaatcgctc gacagggag gcctcccac gtgcagcag gcggcccgc gagctcgag agcaccatc tctgggggt aatgtgagg gcgcgcgac atcacctcg ctgcttgga ggcatggtg gagaagctg caggtgctg aagcgcaaa gccgaggag agcatctcc gaggagctg gccgcctcg aatgtgtgc aagcggcgg ctggagcac ctgaaggag cgcgagacg ctcacgtcg gccgggacg gtctcgcag ggggccgtc aaccagtgg aagaggaag cggctggat aggatgatg gtggagtac ttcctcagg aatgggtat tacaacgct gcgattact ctggcggag aagagcgat atcaaggac ttgaccaat attgagatt tttttgacg tcgcgcgag atcgagaag agtctcgcg aatcatgag acgtccaag tgcttgatt tggtgccat gacaataag tccaaactt cgtaagctt aagtcaaat atggagttt aatttgagg atccaggag tttgttgaa ctgatcagg tctgagcgc agaatggac gctattaag cacgcgaga aagcatttt ccgagcttc gaagacgag catttagat acgattcag agggttatg gcgcttttg gcgtttccg ataggtaca gaaataaaa ccctacaag gccctcttt gacgagacc agatgggac accctcatc gagcaattt agacaagaa aactaccga ctgttccaa ttagcttca caatcagtg tttacagtg gctcttcaa gctggactt tcagcgctt aaaacacca tatcctttg atgcatttt acgtgttgt tattctgat aacggggaa aacaggaat cccgcctgt ccagtttgc caaccttgc ctcaaccaa ttagcagag tctttgcct ttcgcccat tgctcacag tcacgtctt tattgccat attagcgga ttaccacta aacgaaaac aatttaccc atgatgttg cccaatgga tatatttac ggagaacaa gcgctagaa caaatggcg aaggagaat aacgggcaa ataatatgt cccaaaacg aaagagata ttcccgttt aagaaagtc gaaaaagtt tacgttatg taa
- >TC011953 RA
actccttct ttaaaaata ccaaacttg ctaattgta tgtgctttt gccgtgtac tcgcaaatt tcgcgtttc ccgaacttc cctgccatt tctattgac aaaatcagc tgtttgcga agccagcag ctgagtgag tgacgtttt atttatgtt tttatttgt gcaccaaaa tggcagact tgaaatctc tggagcatc ccacgttga aggtgccgt acgagatcc tcaacaaga agttccgga ctgcgcaaa aatcgctcg acagggagg cctcccacg tgcagcagg cggcccgcg agctcgaga gcaccatct ctgggggta atgtgaggg cgcgcgaca tcacctcgc tgcttggag gcatggtgg agaagctgc aggtgctga agcgcaaag ccgaggaga gcatctccg aggagctgg ccgcctcga atgtgtgca agcggcggc tggagcacc tgaaggagc gcgagacgc tcacgtcgg ccgggacgg tctcgcagg gggccgtca accagtgga agaggaagc ggctggata ggatgatgg tggagtact tcctcagga atgggtatt acaacgctg cgattactc tggcggaga agagcgata tcaaggact tgaccaata ttgagattt ttttgacgt cgcgcgaga tcgagaaga gtctcgcga atcatgaga cgtccaagt gcttgattt ggtgccatg acaataagt ccaaacttc gtaagctta agtcaaata tggagttta atttgagga tccaggagt ttgttgaac tgatcaggt ctgagcgca gaatggacg ctattaagc acgcgagaa agcattttc cgagcttcg aagacgagc atttagata cgattcaga gggttatgg cgcttttgg cgtttccga taggtacag aaataaaac cctacaagg ccctctttg acgagacca gatgggaca ccctcatcg agcaattta gacaagaaa actaccgac tgttccaat tagcttcac aatcagtgt ttacagtgg ctcttcaag ctggacttt cagcgctta aaacaccat atcctttga tgcatttta cgtgtgtgt ttttgtact gagtgttgt tccttaaca aaacttaat cagtgttat tctgataac ggggaaaac aggaatccc gcctgtcca gtttgccaa ccttgcctc aaccaatta gcagagtct ttgcctttc gcccattgc tcacagtca cgtctttat tgccatatt agcggatta ccactaaac gaaaacaat ttacccatg atgttgccc aatggatat atttacgga gaacaagta agttgattt tgcgattgg tcggttgtt actggacga cttgcaggc gctagaaca aatggcgaa ggagaataa cgggcaaat aatatgtcc caaaacgaa agagatatt cccgtttaa gaaagtcga aaaagttta cgttatgta aatgtgata ttttctttt ttatagtat tgtttatac ctctctaag ttgagttaa gttaagtac tcatagggt aacagtttt ttgtgaaat atactttat gcttatcg
- >TC011953 RB
actccttct ttaaaaata ccaaacttg ctaattgta tgtgctttt gccgtgtac tcgcaaatt tcgcgtttc ccgaacttc cctgccatt tctattgac aaaatcagc tgtttgcga agccagcag ctgagtgag tgacgtttt atttatgtt tttatttgt gcaccaaaa tggcagact tgaaatctc tggagcatc ccacgttga aggtgccgt acgagatcc tcaacaaga agttccgga ctgcgcaaa aatcgctcg acagggagg cctcccacg tgcagcagg cggcccgcg agctcgaga gcaccatct ctgggggta atgtgaggg cgcgcgaca tcacctcgc tgcttggag gcatggtgg agaagctgc aggtgctga agcgcaaag ccgaggaga gcatctccg aggagctgg ccgcctcga atgtgtgca agcggcggc tggagcacc tgaaggagc gcgagacgc tcacgtcgg ccgggacgg tctcgcagg gggccgtca accagtgga agaggaagc ggctggata ggatgatgg tggagtact tcctcagga atgggtatt acaacgctg cgattactc tggcggaga agagcgata tcaaggact tgaccaata ttgagattt ttttgacgt cgcgcgaga tcgagaaga gtctcgcga atcatgaga cgtccaagt gcttgattt ggtgccatg acaataagt ccaaacttc gtaagctta agtcaaata tggagttta atttgagga tccaggagt ttgttgaac tgatcaggt ctgagcgca gaatggacg ctattaagc acgcgagaa agcattttc cgagcttcg aagacgagc atttagata cgattcaga gggttatgg cgcttttgg cgtttccga taggtacag aaataaaac cctacaagg ccctctttg acgagacca gatgggaca ccctcatcg agcaattta gacaagaaa actaccgac tgttccaat tagcttcac aatcagtgt ttacagtgg ctcttcaag ctggacttt cagcgctta aaacaccat atcctttga tgcatttta cgtgttgtt attctgata acggggaaa acaggaatc ccgcctgtc cagtttgcc aaccttgcc tcaaccaat tagcagagt ctttgcctt tcgcccatt gctcacagt cacgtcttt attgccata ttagcggat taccactaa acgaaaaca atttaccca tgatgttgc ccaatggat atatttacg gagaacaag cgctagaac aaatggcga aggagaata acgggcaaa taatatgtc ccaaaacga aagagatat tcccgttta agaaagtcg aaaaagttt acgttatgt aaatgtgat attttcttt tttatagta ttgtttata cctctctaa gttgagtta agttaagta ctcataggg taacagttt tttgtgaaa tatacttta tgcttatcg
