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Gene information
General information
TC011942 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid67: 3428833..3434709
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011942
    iBeetle screen iB_01907
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011942 RA
      MDGFLKVTN PDLTSSPSN SFIADEVAS ICPLITPSP 
      PTNDLINPL PESNEPNYQ IGEDLATIL QQHVQDEDK 
      CAGDSASSS SSALAEPSC TQLLNHRNS TYHHIAKDE 
      NTANEETAG KVQPLQLTF RNPPENYFF MSWNWPLIR 
      KVSLGMFLS GLVAMVALV IMMISTLPK TCDPYTEWY 
      QGKVFYEIF PASFYASDR NHMYGDFKG IALKSGYLK 
      SLGVRAIRL NSIFQTPNY PDDYKNVTN LLEIAPQLG 
      TIEDFDKMV TEYLKPKNI SLILDLPVF PVLRQLNRR 
      RIEANSTNQ EPAVEFLKQ EPDLMEQVL LHWTRHGVE 
      GFYLKGLEH FIDDPHLGA ALRRWKNIL GPDRPLIIS 
      EGLINAAPK NTINLILNS VDLVDVRLE IEKGLSAVT 
      KQIESVQNG TLFSRRGLP WVHWSLGDE SLPTRLANL 
      LPYGNATLG ATLLQLMLP GTPCIFYGN EIGLQQISD 
      PEGDRTDQK HLHHFTFMA WEERKPSQV LPWMHGERA 
      VSDFGQAKS VADMVELRA KSPAIFYNS IFKEGESKA 
      NSEVIFSRE NLLVIQRWY PRRKSYVVV SNLGNATIT 
      QDLSTLLYS GIVIIGPRT DSKSGSISF KDLSLWPGE 
      AVVIELM
    CDS sequence
    • >TC011942 RA
      atggacggg tttctgaaa gtgacaaac ccagatttg 
      acgagttcg ccctcgaat tcgttcatt gcggatgag 
      gtggcgtcg atttgccct ctgataacg ccttcgccc 
      ccaaccaac gaccttatt aaccctctg ccggagtcg 
      aacgagccc aactaccaa atcggcgag gatttggcc 
      acgattttg caacagcat gtgcaagat gaggataag 
      tgcgcaggg gactcagcc tcctcgagc tcgtcggcg 
      ttagccgaa ccctcgtgc acccaactg ctcaatcac 
      aggaacagc acttatcac catatcgcc aaagacgaa 
      aatacggcc aatgaggaa accgcaggc aaagtccag 
      cccctgcag ctaacgttc cgcaacccc cctgaaaac 
      tacttcttc atgtcgtgg aactggccc ctgatccgc 
      aaggtctcc ctcggcatg ttcctctca ggcctggtg 
      gcgatggtg gccctcgta atcatgatg atctcaact 
      ctgcccaaa acctgcgac ccttacacc gagtggtac 
      caaggaaaa gtcttctac gaaattttc cccgccagt 
      ttctacgcc agcgaccgc aaccacatg tacggcgac 
      ttcaaggga atcgccctc aaaagcggc tacttgaag 
      tcgctgggc gtgcgagcg attcgcctc aactcgatt 
      ttccaaaca cccaactac cccgacgat tacaaaaac 
      gtgacgaat ttgctcgaa atcgccccc cagttgggc 
      acaattgag gactttgac aaaatggtc acggagtat 
      ctgaagccg aagaacatc tcgctcatt cttgattta 
      ccggttttt ccggttttg aggcaactc aaccggagg 
      agaattgag gcgaactcg accaaccag gagccagcg 
      gttgagttt ttgaaacag gaaccggat ttaatggaa 
      caggtttta ctccactgg acgaggcac ggagttgag 
      gggttctac ctcaaaggt ttggagcac tttattgac 
      gacccccac ttgggggct gccctgcga cgttggaaa 
      aacattcta gggcctgac cggccctta atcatcagc 
      gagggcctc attaacgcg gccccaaaa aacacaatc 
      aatttaatt ttgaacagt gtggaccta gttgacgtg 
      cgactggag atagagaag ggcctttcg gccgtcacc 
      aaacaaatc gagtcggtt caaaacggg acgcttttc 
      tcccgccga ggcctcccg tgggtgcac tggagcctg 
      ggggacgag tccctcccg actcgcctc gccaatctc 
      ctcccttac gggaacgcg accttgggg gcaaccctc 
      ctccagttg atgctccca gggaccccg tgcattttc 
      tacgggaat gagatcggc ctccagcag atttcagac 
      cctgaaggc gaccgcacc gaccagaaa cacctccac 
      cacttcacc ttcatggcc tgggaggag cggaagccc 
      agccaggtc ctgccatgg atgcacggg gagagggcg 
      gtttccgac tttggccag gccaagtct gtggcggac 
      atggtggag ttgagggcg aaatcgccg gcgattttt 
      tacaactcg atttttaaa gagggggag agcaaggcc 
      aactcggag gtgattttt tcgcgggag aatttgttg 
      gtgatccag cggtggtat cccaggagg aagagttac 
      gtggttgtg agcaatcta gggaatgcg accattacg 
      caggatttg tccacgctg ctgtacagc gggattgtg 
      atcattggg cccaggacg gactccaag tcggggagc 
      atctccttc aaggatctt tcgttgtgg ccgggggag 
      gcggtcgtt attgaactt atgtaa
    mRNA sequence
    • >TC011942 RA
      ttattttct aatttgtca cgccacaaa gcacaattt 
      ttcattccg acttatcac acaaacaga atttttggc 
      cgctcttcc aaaaacttg accttgcct ccacaaaca 
      atccacttc caacgaatt ttcaaactt ggcgccgga 
      cgcgtcgcg acgcccagc gccgcgacg tgcccataa 
      tagtcgatc agctgagcg ccgatcaca tgaactcaa 
      tcgaacaaa ttagtgctt gtgtgcgac aatggacgg 
      gtttctgaa agtgacaaa cccagattt gacgagttc 
      gccctcgaa ttcgttcat tgcggatga ggtggcgtc 
      gatttgccc tctgataac gccttcgcc cccaaccaa 
      cgaccttat taaccctct gccggagtc gaacgagcc 
      caactacca aatcggcga ggatttggc cacgatttt 
      gcaacagca tgtgcaaga tgaggataa gtgcgcagg 
      ggactcagc ctcctcgag ctcgtcggc gttagccga 
      accctcgtg cacccaact gctcaatca caggaacag 
      cacttatca ccatatcgc caaagacga aaatacggc 
      caatgagga aaccgcagg caaagtcca gcccctgca 
      gctaacgtt ccgcaaccc ccctgaaaa ctacttctt 
      catgtcgtg gaactggcc cctgatccg caaggtctc 
      cctcggcat gttcctctc aggcctggt ggcgatggt 
      ggccctcgt aatcatgat gatctcaac tctgcccaa 
      aacctgcga cccttacac cgagtggta ccaaggaaa 
      agtcttcta cgaaatttt ccccgccag tttctacgc 
      cagcgaccg caaccacat gtacggcga cttcaaggg 
      aatcgccct caaaagcgg ctacttgaa gtcgctggg 
      cgtgcgagc gattcgcct caactcgat tttccaaac 
      acccaacta ccccgacga ttacaaaaa cgtgacgaa 
      tttgctcga aatcgcccc ccagttggg cacaattga 
      ggactttga caaaatggt cacggagta tctgaagcc 
      gaagaacat ctcgctcat tcttgattt accggtttt 
      tccggtttt gaggcaact caaccggag gagaattga 
      ggcgaactc gaccaacca ggagccagc ggttgagtt 
      tttgaaaca ggaaccgga tttaatgga acaggtttt 
      actccactg gacgaggca cggagttga ggggttcta 
      cctcaaagg tttggagca ctttattga cgaccccca 
      cttgggggc tgccctgcg acgttggaa aaacattct 
      agggcctga ccggccctt aatcatcag cgagggcct 
      cattaacgc ggccccaaa aaacacaat caatttaat 
      tttgaacag tgtggacct agttgacgt gcgactgga 
      gatagagaa gggcctttc ggccgtcac caaacaaat 
      cgagtcggt tcaaaacgg gacgctttt ctcccgccg 
      aggcctccc gtgggtgca ctggagcct gggggacga 
      gtccctccc gactcgcct cgccaatct cctccctta 
      cgggaacgc gaccttggg ggcaaccct cctccagtt 
      gatgctccc agggacccc gtgcatttt ctacgggaa 
      tgagatcgg cctccagca gatttcaga ccctgaagg 
      cgaccgcac cgaccagaa acacctcca ccacttcac 
      cttcatggc ctgggagga gcggaagcc cagccaggt 
      cctgccatg gatgcacgg ggagagggc ggtttccga 
      ctttggcca ggccaagtc tgtggcgga catggtgga 
      gttgagggc gaaatcgcc ggcgatttt ttacaactc 
      gatttttaa agaggggga gagcaaggc caactcgga 
      ggtgatttt ttcgcggga gaatttgtt ggtgatcca 
      gcggtggta tcccaggag gaagagtta cgtggttgt 
      gagcaatct agggaatgc gaccattac gcaggattt 
      gtccacgct gctgtacag cgggattgt gatcattgg 
      gcccaggac ggactccaa gtcggggag catctcctt 
      caaggatct ttcgttgtg gccggggga ggcggtcgt 
      tattgaact tatgtaatt tttagagca ttgtgtatt 
      ttttctgtg ataaatggt taaatcatt gattgaagt 
      ataaataaa gctaattgt ctatt