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Gene information
General information
TC011911 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 11738485..11740172
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011911
    iBeetle screen iB_01902

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011911 RA
      MADRKHTRE ILTVLILCV LWYVVSSSN NVIGKTLLN 
      EFPYPMTMT MVQLLSITV FSGPLFNLW GIRKYADIS 
      WRYYFTLIV PLAFGKFIA SVFSHVSIW KVPVSYAHT 
      VKATMPLFT VVLSRILMK EKQTLRVYF SLIPIITGV 
      AIATITEIS FDVIGLISA LVATMGFSL MNIFSKKVL 
      HDTNVHHLR LLHILGRLA LVMFLPVWV LVDMFRLLK 
      DDTVKYHDY RVIGLLIMD GVLNWLQNI IAFSVLSLV 
      TPLTYAVAN ASKRIFVIA VSLFILGNP VTGTNVFGM 
      LLAIFGVLL YNKAKYDAK QAEKKQTIL PYSQNSWQD 
      RTGYTVLQN SEPVKPANG FTVVNGSNN NSFVYNSKS 
      SSSNLLFV
    CDS sequence
    • >TC011911 RA
      atggctgac cgcaaacac actcgagaa atactcacc 
      gtattaatc ctgtgtgtt ttatggtac gttgtcagc 
      tcgagcaac aatgtcatc ggcaagacc cttctcaac 
      gaatttccg tacccgatg acgatgaca atggtccag 
      ctgctgtca atcaccgtt tttagtggc ccccttttc 
      aacttgtgg ggcattcgc aagtacgcg gacatctcc 
      tggaggtac tacttcact ctaattgtt cctctagcc 
      ttcgggaag ttcatagcg tccgtattc tcgcatgtc 
      agtatttgg aaagtgcca gtttcttac gcacacaca 
      gttaaggcc actatgcct cttttcacc gtcgttctc 
      tcgaggatc ctcatgaag gagaagcag acgttgagg 
      gtttatttt agtctgatc ccgattatt acgggggtt 
      gcgattgcg accatcact gaaatcagt ttcgatgta 
      attgggtta attagtgct ttggttgca accatgggt 
      ttctccctt atgaatatt ttctcgaaa aaagtcttg 
      cacgacacc aacgtgcac cacttgcgg ctgttgcac 
      attttgggg cgactggcc ctcgtcatg ttcctccca 
      gtgtgggtc cttgttgac atgttccga cttttgaaa 
      gacgacacc gtcaaatac cacgactat cgagtcatt 
      ggactcttg atcatggac ggagtgctc aactggctc 
      cagaacatc atcgctttc agtgttctg tctctagtg 
      actcccttg acttacgct gtggctaac gccagtaaa 
      cgaattttc gtaatcgcg gtttcgtta tttattcta 
      ggcaatccc gtgactggg accaacgtt ttcgggatg 
      cttttagca attttcggg gttttattg tacaataag 
      gcgaaatac gacgccaag caagctgag aagaagcaa 
      actattctt ccctacagc caaaacagt tggcaagac 
      cggacgggt tataccgtc ctacaaaac agcgagccg 
      gttaaaccc gctaacggt ttcaccgtc gtcaacggc 
      tcaaataat aattcgttt gtctacaac tcaaagtct 
      agctctagt aatttattg tttgtgtga
    mRNA sequence
    • >TC011911 RA
      gcagatgtc atgttgacg tcccattga cagttgtga 
      ctgccagca tggctgacc gcaaacaca ctcgagaaa 
      tactcaccg tattaatcc tgtgtgttt tatggtacg 
      ttgtcagct cgagcaaca atgtcatcg gcaagaccc 
      ttctcaacg aatttccgt acccgatga cgatgacaa 
      tggtccagc tgctgtcaa tcaccgttt ttagtggcc 
      cccttttca acttgtggg gcattcgca agtacgcgg 
      acatctcct ggaggtact acttcactc taattgttc 
      ctctagcct tcgggaagt tcatagcgt ccgtattct 
      cgcatgtca gtatttgga aagtgccag tttcttacg 
      cacacacag ttaaggcca ctatgcctc ttttcaccg 
      tcgttctct cgaggatcc tcatgaagg agaagcaga 
      cgttgaggg tttatttta gtctgatcc cgattatta 
      cgggggttg cgattgcga ccatcactg aaatcagtt 
      tcgatgtaa ttgggttaa ttagtgctt tggttgcaa 
      ccatgggtt tctccctta tgaatattt tctcgaaaa 
      aagtcttgc acgacacca acgtgcacc acttgcggc 
      tgttgcaca ttttggggc gactggccc tcgtcatgt 
      tcctcccag tgtgggtcc ttgttgaca tgttccgac 
      ttttgaaag acgacaccg tcaaatacc acgactatc 
      gagtcattg gactcttga tcatggacg gagtgctca 
      actggctcc agaacatca tcgctttca gtgttctgt 
      ctctagtga ctcccttga cttacgctg tggctaacg 
      ccagtaaac gaattttcg taatcgcgg tttcgttat 
      ttattctag gcaatcccg tgactggga ccaacgttt 
      tcgggatgc ttttagcaa ttttcgggg ttttattgt 
      acaataagg cgaaatacg acgccaagc aagctgaga 
      agaagcaaa ctattcttc cctacagcc aaaacagtt 
      ggcaagacc ggacgggtt ataccgtcc tacaaaaca 
      gcgagccgg ttaaacccg ctaacggtt tcaccgtcg 
      tcaacggct caaataata attcgtttg tctacaact 
      caaagtcta gctctagta atttattgt ttgtgtgag 
      acacgttaa gcttagcca taattttta atgtttatg 
      acgggattt cagtcctgt ggcaataac aaatgttat 
      ctcttgatt gtttgaggt ttaagatgt aaacgaaag 
      ttctcgcta gtcacgaaa gtgcttttt acgaatgtt 
      accaatctc gaaatttac ttgttttgt tggtgctca 
      actacttat tacgtctct aaagattcc tttaattat 
      tgttaagta ggtaatgaa cgtgtacct aactgtaca 
      tactagctg tattttatt tatttgtat actcaagta 
      ttgtggact gtatgcagt cgcggttga ttttttatt 
      ataccaacg tttggtcat acagtattt atcggaact 
      gtacataaa aaggaaata aatattttt atacaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY