Status
Please wait ...
Gene information
General information
TC011895 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12141682..12144202
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011895
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01901

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: GFP-positive tube-like structure present 30-50% (potentially gut?)
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): more than 80%

    cuticle remnants without visible segmentation but polarity - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011895 RA
      MDPSVEIRK LPLDVRSHL ISLLELQDS WKKLMAIIP 
      QTLSLNEYK CDINFNNPA KYRSEHFHI IEDNSVKKR 
      IFCSEILLR EWGTSGRVR PAVGHLKYL LQKAELFRA 
      ADYVAVDIL KEEPPPRPA KGPAAPVVF NPDELLDPE 
      TKRIEKILD EINYPSSAI ENINKLNSF NTNLNYSGK 
      ETTKVIPKI IISESPDVE ETPPPFRLP VVVQKVKSE 
      SDLIKFSQE SVSNSDNNA NVPHLSSLL TDEASNNNI 
      PAVISDLVS QSSSETTEN ADSAVNRPE LSSLLNQSS 
      DDSEPNFNV PAISELSLA SSSDSSSNF PALSQLIDS 
      EKNSRSCSS PLPNLSLNT PLPHFSYCD LATATNNFD 
      ETPVAQGGR FLGSGAFGK VYLGVGLVS KPVAVKKVI 
      LENVDVVRV DDMVTKQFR NEVEILSKY EHENLVALR 
      GYSCDGDTY CLLYEYVPG GALKDRLQK HELIWTERL 
      YVAMGTARA IAYLHTAYA SPLIHRDVK SANILLDSN 
      NKPKLCDFG LIKRALNQN TNTATTIFG TSAYMAPEA 
      FRGDVSVKL DTFSFGVVL LELLTSMPP MDESREGVD 
      LVTHVQETC QDGIKPLLD TTVGSWMAN SINFGEKLY 
      EIATSCLKE KKERPTMTS ICGILGDLV QKFL
    CDS sequence
    • >TC011895 RA
      atggatccg tccgttgaa atcagaaaa ttgcctttg 
      gacgtgcgc tcccacctc ataagcctt ttggaattg 
      caggactct tggaaaaaa ttgatggcc ataataccg 
      caaacactg tctctaaac gagtataaa tgcgatata 
      aacttcaat aacccggcc aaataccgg tcggagcac 
      ttccacata attgaggac aactccgtc aagaagcga 
      attttctgc tcggagatt ttgttgcgc gagtggggc 
      acctcaggt cgggtcagg cccgccgtg ggccacctg 
      aaatatctc ctgcagaag gcagagctg tttcgtgcg 
      gctgactac gtcgctgtt gatatttta aaggaggag 
      ccgcctcct aggcccgcc aaagggcca gcggcccca 
      gttgtgttc aatccggac gagttacta gacccggag 
      acgaaacga atcgagaaa attctggac gagatcaac 
      taccccagt tccgcaatc gagaatatc aacaaactg 
      aattcgttt aacacaaac ttgaattac agcggcaaa 
      gaaacgacg aaagtgatc cccaagatt ataatcagc 
      gaatcaccg gatgtggaa gagacgccg ccgccgttt 
      cggctaccg gtcgttgtc cagaaagtc aagtctgag 
      tcggatttg attaagttt tcgcaagaa tcggtttca 
      aacagcgat aataacgca aatgtgccg catttgtcg 
      tcgcttttg acggatgag gcgtccaat aataacatt 
      cctgccgta attagcgat ttagtgtca caatcgagc 
      tcagaaacc actgaaaat gccgattct gcggtcaac 
      agaccggag ttatcgtca cttttgaac cagtcaagt 
      gacgactct gagcccaat ttcaacgtt cctgcaatc 
      agcgaattg agtctggcg tcaagttcc gactcttcg 
      tcaaacttc cccgctttg agtcaatta atcgattct 
      gagaaaaat tcgcggtcg tgttcgtcg ccgctcccg 
      aacttgtcg ctgaacact cctttgcca cactttagt 
      tactgtgat ttagcaacg gcgactaat aacttcgac 
      gagacaccg gtggcccaa gggggcagg tttttgggg 
      tcgggggct ttcggtaaa gtttatttg ggtgtgggg 
      ttggttagt aaaccagtg gcggtgaag aaagtcatt 
      ttggaaaat gttgacgtc gttcgagtt gatgatatg 
      gttactaaa cagttccgc aacgaagtg gagattttg 
      agtaaatat gagcatgag aatttggtg gctttgagg 
      ggctattcg tgcgatggc gacacgtat tgcttgttg 
      tacgagtac gtccctgga ggggcactc aaggaccgg 
      ctacagaaa catgagttg atttggacg gaaaggctg 
      tatgtagca atggggaca gcgagggcg attgcgtat 
      ctccacact gcatacgca agccccttg attcaccgt 
      gacgttaaa tctgcaaat attttactc gacagtaac 
      aataaacca aagttgtgc gactttggg ctaattaaa 
      cgcgctctc aaccaaaat acaaacacc gcaactaca 
      attttcggg acttcggct tacatggca cctgaggct 
      ttcagggga gacgtttca gtaaagttg gacactttc 
      agttttgga gtcgttttg cttgaacta ctcacttca 
      atgcctcca atggacgaa agccgggag ggagtcgat 
      ttggttact catgtgcaa gaaacctgc caggacggt 
      attaaacca ttgttagac acaacagtt ggctcatgg 
      atggccaat agtattaat ttcggcgag aaattatac 
      gagattgcg acgagttgt ttaaaggag aagaaagag 
      agacccact atgacatca atttgcggt attttaggc 
      gatctagtc cagaaattt ttataa
    mRNA sequence
    • >TC011895 RA
      acaaaaaaa tattcaaat ttcaaacgt aacgccaat 
      ccgtaatat tatagtaat gcccaccag aggcgctgt 
      gttcgtgtc aattattag ggaattctc ccttctaaa 
      gttgtaatg tcagtgtca gtgtcagtt tctgtgttt 
      agaggttag atttttgtt tttgtaaat aaaaagtgc 
      cttaattac ccgtccaac caacggaat tgcaatgga 
      tccgtccgt tgaaatcag aaaattgcc tttggacgt 
      gcgctccca cctcataag ccttttgga attgcagga 
      ctcttggaa aaaattgat ggccataat accgcaaac 
      actgtctct aaacgagta taaatgcga tataaactt 
      caataaccc ggccaaata ccggtcgga gcacttcca 
      cataattga ggacaactc cgtcaagaa gcgaatttt 
      ctgctcgga gattttgtt gcgcgagtg gggcacctc 
      aggtcgggt caggcccgc cgtgggcca cctgaaata 
      tctcctgca gaaggcaga gctgtttcg tgcggctga 
      ctacgtcgc tgttgatat tttaaagga ggagccgcc 
      tcctaggcc cgccaaagg gccagcggc cccagttgt 
      gttcaatcc ggacgagtt actagaccc ggagacgaa 
      acgaatcga gaaaattct ggacgagat caactaccc 
      cagttccgc aatcgagaa tatcaacaa actgaattc 
      gtttaacac aaacttgaa ttacagcgg caaagaaac 
      gacgaaagt gatccccaa gattataat cagcgaatc 
      accggatgt ggaagagac gccgccgcc gtttcggct 
      accggtcgt tgtccagaa agtcaagtc tgagtcgga 
      tttgattaa gttttcgca agaatcggt ttcaaacag 
      cgataataa cgcaaatgt gccgcattt gtcgtcgct 
      tttgacgga tgaggcgtc caataataa cattcctgc 
      cgtaattag cgatttagt gtcacaatc gagctcaga 
      aaccactga aaatgccga ttctgcggt caacagacc 
      ggagttatc gtcactttt gaaccagtc aagtgacga 
      ctctgagcc caatttcaa cgttcctgc aatcagcga 
      attgagtct ggcgtcaag ttccgactc ttcgtcaaa 
      cttccccgc tttgagtca attaatcga ttctgagaa 
      aaattcgcg gtcgtgttc gtcgccgct cccgaactt 
      gtcgctgaa cactccttt gccacactt tagttactg 
      tgatttagc aacggcgac taataactt cgacgagac 
      accggtggc ccaaggggg caggttttt ggggtcggg 
      ggctttcgg taaagttta tttgggtgt ggggttggt 
      tagtaaacc agtggcggt gaagaaagt cattttgga 
      aaatgttga cgtcgttcg agttgatga tatggttac 
      taaacagtt ccgcaacga agtggagat tttgagtaa 
      atatgagca tgagaattt ggtggcttt gaggggcta 
      ttcgtgcga tggcgacac gtattgctt gttgtacga 
      gtacgtccc tggaggggc actcaagga ccggctaca 
      gaaacatga gttgatttg gacggaaag gctgtatgt 
      agcaatggg gacagcgag ggcgattgc gtatctcca 
      cactgcata cgcaagccc cttgattca ccgtgacgt 
      taaatctgc aaatatttt actcgacag taacaataa 
      accaaagtt gtgcgactt tgggctaat taaacgcgc 
      tctcaacca aaatacaaa caccgcaac tacaatttt 
      cgggacttc ggcttacat ggcacctga ggctttcag 
      gggagacgt ttcagtaaa gttggacac tttcagttt 
      tggagtcgt tttgcttga actactcac ttcaatgcc 
      tccaatgga cgaaagccg ggagggagt cgatttggt 
      tactcatgt gcaagaaac ctgccagga cggtattaa 
      accattgtt agacacaac agttggctc atggatggc 
      caatagtat taatttcgg cgagaaatt atacgagat 
      tgcgacgag ttgtttaaa ggagaagaa agagagacc 
      cactatgac atcaatttg cggtatttt aggcgatct 
      agtccagaa atttttata aaagaatat attccatca 
      aatgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY